LOCUS       sequence-211250-       12875 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lenti-viral expression of Flag-tagged Depdc5 mutant AB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12875)
  AUTHORS   Shen K, Huang RK, Brignole EJ, Condon KJ, Valenstein ML, 
            Chantranupong L, Bomaliyamu A, Choe A, Hong C, Yu Z, Sabatini DM
  TITLE     Architecture of the human GATOR1 and GATOR1-Rag GTPases complexes.
  JOURNAL   Nature. 2018 Apr 5;556(7699):64-69. doi: 10.1038/nature26158. Epub 
            2018 Mar 28.
  PUBMED    29590090
REFERENCE   2  (bases 1 to 12875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature26158"; journalName: "Nature"; date: "2018-04-5- 5"; 
            volume: "556"; issue: "7699"; pages: "64-69"
COMMENT     Sequence Label: [pSK497] pLJC5 Flag-Depdc5(AB)
FEATURES             Location/Qualifiers
     source          1..12875
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    288..309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(324..329,330..347,348..354)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    362..378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     367..389
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     386..402
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     386..402
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     421..441
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        423..441
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        469..695
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             696..876
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    923..1048
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1541..1774
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1959..2003
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2292..3503
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3340..3359
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3524..3533
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3536..3559
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    8437..8554
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8603..9113
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9032..9050
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9135..9734
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9135..9154)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9631..9651
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9862..10095
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10167..10288
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10204..10223)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10258..10277
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10328..10463
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10483..10502)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10484..10502)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10512..10529)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10512..10528)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10521..10543)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10670..11125
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10757..10776)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10967..10988
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11151..11255
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11256..11324,11325..12116)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11474..11493)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12287..12875
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12776..12795
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      241 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      301 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      361 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
      421 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
      481 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
      541 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
      601 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
      661 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
      721 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
      781 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
      841 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
      901 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
      961 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     1021 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     1081 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     1141 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     1201 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1261 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1321 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1381 gcaagcagca gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     1441 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     1501 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     1561 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     1621 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     1681 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     1741 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     1801 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     1861 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     1921 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     1981 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     2041 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     2101 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     2161 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     2221 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgatcacga gactagcctc
     2281 gagcgggatc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac
     2341 ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac
     2401 gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa
     2461 ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg
     2521 aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg
     2581 tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc
     2641 gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg
     2701 gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt
     2761 ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt
     2821 tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag
     2881 gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag aacccaaggt
     2941 cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct ggggcaccat
     3001 ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc gtctgttgcg
     3061 ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg agcgcgcgcc
     3121 ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg ggccacctgc
     3181 cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc ctagggtagg
     3241 ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg aggcgtcagt
     3301 ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg ttttgaacta
     3361 tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt gaaatgtaat
     3421 catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc
     3481 cgtttttggc ttttttgtta gacgaagcgc tagcgctacc ggtgccacca tggaggacta
     3541 caaggatgac gatgacaagg gtggtggtgg tggtgcgtcg acgatgagaa caacaaaggt
     3601 ctacaaactc gtcatccaca agaagggctt tgggggcagt gatgatgagc tagttgtgaa
     3661 ccccaaagtg ttccctcaca tcaagcttgg agacattgta gagattgcac accccaacga
     3721 tgaatacagc cctctgcttt tgcaggtcaa gtctcttaag gaagatttac agaaggaaac
     3781 tatcagtgtg gaccagactg tgactcaagt gttccggctg agaccttatc aggatgtcta
     3841 tgttaatgtc gtagacccta aggatgtgac ccttgaccta gtggaattaa cttttaagga
     3901 tcagtatatt ggccgtgggg atatgtggcg actaaagaaa agtttggtca gcacatgtgc
     3961 ctatatcacc cagaaggtgg agtttgctgg catcagagca caggctggtg aactgtgggt
     4021 taagaatgag aaggtcatgt gtggctacat cagtgaagat accagggtgg tgtttcgttc
     4081 tacgtcggct atggtttaca tatttattca gatgagctgt gaaatgtggg attttggcag
     4141 cggaagcggc ttgtattttg agaaagctgt gaatggtttc cttgctgatc tatttaccaa
     4201 gtggaaggag aagaactgta gtcatgaagt gacagtggtc ctgttttcta gaactttcta
     4261 tgatgcaaaa tctgttgatg aatttcctga aataaaccga gcctcaattc gacaggatca
     4321 caaggggaga ttctatgaag acttttacaa agtggtggtg cagaatgaga gaagagaaga
     4381 atggacttca cttctcgtaa ccattaaaaa actcttcatc cagtatccag tgttggtgcg
     4441 actggaacag gcagagggct ttcctcaagg agataattct acctcagcac aaggaaacta
     4501 cctggaggcc atcaatctgt cattcaatgt gtttgataag cactacatca accgcaactt
     4561 tgaccgaact gggcagatgt cagtggtgat cacgcccggg gtgggtgtct ttgaagtgga
     4621 ccgcctactc atgatcctga ccaagcagcg gatgatagat aatggaattg gtgtggattt
     4681 ggtgtgcatg ggaggcagcg gaagcggcgc tgtcccattg ttcaagctcc ataatcggag
     4741 tgctccccgt gattctcgtc tgggcgatga ctataatatc cctcactgga taaaccacag
     4801 tttctacaca tccaaaagcc agctcttttg taatagtttc accccacgaa taaaactggc
     4861 aggaaagaag cccgcctctg agaaagcaaa aaatggccgt gatacatctc tcgggagtcc
     4921 aaaagaatct gagaacgccc ttcccatcca agtagattat gacgcctatg acgctcaagt
     4981 gttcaggctg cccggcccat cccgggccca gtgcctcacc acctgcagat ctgtgcgaga
     5041 gcgagagagt cacagtcgaa agagtgccag ctcctgtgat gtttcatcca gcccttccct
     5101 accaagccgc acactgccca ctgaggaagt gaggagccag gcttctgacg acagctccct
     5161 aggcaagagt gccaacatcc tgatgatccc acacccccac ctgcaccagt atgaagtcag
     5221 cagctccttg ggatacacca gcactcgaga tgtcctggag aacatgatgg agccaccaca
     5281 gcgagactcc agtgcaccag ggaggtttca cgttggcagt gcagaatcca tgctgcatgt
     5341 tcgacctggt ggatacacgc cccagagagc actgattaac cccttcgctc cctctcggat
     5401 gcccatgaag cttacgtcca acagaaggcg ctggatgcac acttttcctg tggggccatc
     5461 cggagaagcc atccagatcc accaccagac ccgacagaat atggcggagc tacaaggcag
     5521 cgggcagagg gatccaactc actcctctgc agagctgctg gagttagcat atcatgaagc
     5581 tgctggaagg cacagcaatt cccgccagcc tggtgacggc atgtccttct tgaacttcag
     5641 tggaacagag gagctttctg tcggcctgct tagcaacagt ggtgcaggta tgaatcctag
     5701 gacccagaat aaggattctc tagaggacag tgtttctacc tctccagacc caattctgac
     5761 actgtctgct ccccctgtag tgccaggctt ctgttgcaca gttggagtgg actggaagtc
     5821 tctcactact ccggcgtgcc tcccccttac caccgactac ttccctgacc gccagggcct
     5881 gcagaatgac tacacagagg gctgttatga tctccttcca gaagcagaca tcgacaggag
     5941 ggacgaagat ggtgtgcaga tgacagccca gcaggtattt gaagagttta tttgccaacg
     6001 tctcatgcag ggctaccaaa tcatagtgca gcccaagaca cagaaaccca atcctgctgt
     6061 cccgcccccg ctgagcagta gcccactcta tagccgaggc cttgtgtccc gaaaccgccc
     6121 tgaggaggag gaccagtatt ggctgagtat gggcagaacg ttccacaaag tgacgctgaa
     6181 ggataagatg atcacagtga cgcgatacct tcccaagtat ccttatgaat ctgcccagat
     6241 ccactacacc tacagcctct gtccttccca ctcagactca gagttcgtct cctgctgggt
     6301 ggagttttcc cacgaacggc tggaggagta caagtggaat tacttagatc agtatatctg
     6361 ttctgccggc tctgaagact tcagcttaat tgagtccctg aagttctgga ggacccgctt
     6421 cctgctgctg ccagcctgtg tcaccgccac caagcgcatc acggaggggg aggcccactg
     6481 cgacatctat ggggacaggc cccgtgcaga cgaggacgag tggcaactcc tggatggttt
     6541 tgtccgcttt gtggagggct tgaatcgcat tcgcaggcgg catcgctcgg atcgcatgat
     6601 gcggaaaggg accgccatga aaggcttgca gatgactggg cccatttcca cgcattctct
     6661 ggagtcaact gcacccccag tggggaagaa gggaacctca gctctctctg ccctgttgga
     6721 gatggaggcc agtcagaagt gcctgggaga acagcaggca gctgtgcatg gtgggaagag
     6781 ctccgcccag tcagccgaga gcagcagcgt tgccatgact cccacctaca tggacagccc
     6841 acgaaaggac ggggccttct ttatggagtt tgtccgcagc ccacgcacag catcgtccgc
     6901 cttctaccct caggtatctg tggaccaaac agccactcct atgttggacg gcaccagttt
     6961 gggcatatgc acaggccaat ccatggacag aggcaacagc cagacctttg ggaactccca
     7021 gaacatagga gaacagggct actcctccac aaactccagt gacagcagct ctcagcagct
     7081 ggtggcaagc tccttgacct catcctctac cctgacagag atcctggaag ccatgaagca
     7141 cccctcgaca ggagtccagc tgctctctga acagaagggc ctctcaccgt actgcttcat
     7201 cagcgcggag gtggtacact ggttggtgaa ccacgtggag gggatccaga cacaggcgat
     7261 ggccattgac atcatgcaga aaatgctgga agagcagctc atcacacatg catctggcga
     7321 agcctggcgg accttcatct acggcttcta tttctacaag atagtaacgg acaaagagcc
     7381 cgaccgagtg gccatgcagc agcccgccac cacctggcac acagcaggag tggacgactt
     7441 cgccagcttc cagcgcaagt ggtttgaggt ggcctttgtg gcagaagagc tcgtgcactc
     7501 tgagattcct gcctttctcc tgccctggct gcctagccgg ccagcctcct atgcaagtag
     7561 gcacagctcc tttagccgaa gttttggagg acggagccag gcggcagcac ttttagctgc
     7621 cactgtccca gagcagagga ctgtgaccct ggatgttgac gtgaacaacc gcacagaccg
     7681 gctggagtgg tgcagctgtt attaccatgg caacttttct ctgaatgcag cctttgagat
     7741 caagctgcac tggatggcgg tgaccgcagc agtactcttc gagatggtcc aaggttggca
     7801 tcggaaagcc acctcctgtg gcttcttgtt agtcccagtt ttggaggggc cttttgcact
     7861 gcccagttac ctgtatggcg acccccttcg tgcccagctc ttcatcccac tcaacatcag
     7921 ctgcttgctc aaggagggca gcgagcacct gtttgatagc tttgaacccg aaacgtactg
     7981 ggatcgaatg cacctcttcc aggaagccat tgcacacagg tttgggtttg tacaagataa
     8041 atattctgcc tctgctttta acttccctgc tgagaacaag cctcagtata tccacgttac
     8101 aggaacagtg tttctgcagc tgccctactc caagcgcaag ttctcagggc agcagcggcg
     8161 gcggcggaac tccaccagct ccaccaacca gaacatgttc tgcgaggagc gggtcggcta
     8221 caactgggcc tacaacacca tgctcaccaa aacatggcgc tccagcgcca caggggatga
     8281 aaagtttgct gatcggctgc tgaaggactt cacggacttc tgcatcaacc gtgacaaccg
     8341 gctggtcacg ttctggacaa gttgcctgga gaagatgcat gccagtgccc cgtgagaatt
     8401 ctcgacctcg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     8461 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     8521 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     8581 tccactttgg ccgcggctcg agggggttgg ggttgcgcct tttccaaggc agccctgggt
     8641 ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg
     8701 ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg
     8761 tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg
     8821 cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac
     8881 agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct
     8941 cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg
     9001 cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc
     9061 gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggatc
     9121 caccggagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     9181 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     9241 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     9301 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     9361 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     9421 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     9481 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     9541 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     9601 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     9661 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     9721 agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac
     9781 cccatgcatc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     9841 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     9901 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     9961 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
    10021 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
    10081 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
    10141 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
    10201 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    10261 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
    10321 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
    10381 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
    10441 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
    10501 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
    10561 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
    10621 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
    10681 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
    10741 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
    10801 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
    10861 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
    10921 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
    10981 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
    11041 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
    11101 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
    11161 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
    11221 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
    11281 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
    11341 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
    11401 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
    11461 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
    11521 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
    11581 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
    11641 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
    11701 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
    11761 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
    11821 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
    11881 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
    11941 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
    12001 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
    12061 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    12121 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    12181 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    12241 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    12301 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    12361 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    12421 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    12481 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    12541 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    12601 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    12661 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    12721 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    12781 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    12841 tgtgatgctc gtcagggggg cggagcctat ggaaa
//
