LOCUS       sequence-211251-        2388 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cation channelrhodopsin ChroME targeted to the neuronal soma and 
            proximal dendrites and separated from nuclear mRuby3 by P2A in a 
            viral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Mardinly AR, Oldenburg IA, Pegard NC, Sridharan S, Lyall EH, Chesnov
            K, Brohawn SG, Waller L, Adesnik H
  TITLE     Precise multimodal optical control of neural ensemble activity.
  JOURNAL   Nat Neurosci. 2018 Apr 30. pii: 10.1038/s41593-018-0139-8. doi: 
            10.1038/s41593-018-0139-8.
  PUBMED    29713079
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2018 Apr 30. pii: 10.1038/s41593-018-0139-8. doi: 
            10.1038/s41593-018-0139-8."
COMMENT     Sequence Label: pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3
FEATURES             Location/Qualifiers
     source          1..2388
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             991..1014
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1225..1281
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1288..1665
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             1675..2385
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
ORIGIN
        1 atggaaacag ccgccacaat gacccacgcc tttatctcag ccgtgcctag cgccgaagcc
       61 acaattagag gcctgctgag cgccgcagca gtggtgacac cagcagcaga cgctcacgga
      121 gaaacctcta acgccacaac agccggagcc gatcacggtt gcttccccca catcaaccac
      181 ggaaccgagc tgcagcacaa gatcgcagtg ggactccagt ggttcaccgt gatcgtggct
      241 atcgtgcagc tcatcttcta cggttggcac agcttcaagg ccacaaccgg ctgggaggag
      301 gtctacgtct gcgtgatcga gctcgtcaag tgcttcatcg agctgttcca cgaggtcgac
      361 agcccagcca cagtgtacca gaccaacgga ggagccgtga tttggctgcg gtacagcgag
      421 tggctcctga cttgccccgt gatcctgatc cacctgagca acctgaccgg actgcacgaa
      481 gagtacagca agcggaccat gaccatcctg gtgaccgaca tcggcaacat cgtgtggggg
      541 atcacagccg cctttacaaa gggccccctg aagatcctgt tcttcatgat cggcctgttc
      601 tacggcgtga cttgcttctt ccagatcgcc aaggtgtata tcgagagcta ccacaccctg
      661 cccaaaggcg tctgccggaa gatttgcaag atcatggcct acgtcttctt ctgctcttgg
      721 ctgatgttcc ccgtgatgtt catcgccgga cacgagggac tgggcctgat cacaccttac
      781 accagcggaa tcggccacct gatcctggat ctgatcagca agaacacttg gggcttcctg
      841 ggccaccacc tgagagtgaa gatccacgag cacatcctga tccacggcga catccggaag
      901 acaaccacca tcaacgtggc cggcgagaac atggagatcg agaccttcgt cgacgaggag
      961 gaggagggag gagtggcggc cgcaccggta gattacaaag acgatgacga taagcagtcc
     1021 cagcccatcc tcaacaccaa ggagatggcc ccgcagagca agcctccaga ggagctggag
     1081 atgagcagca tgcccagccc cgtggcccct ctgcccgcac gcacggaggg cgtcatcgac
     1141 atgcggagca tgtccagcat tgacagcttc atcagctgtg ccacggactt ccctgaagcc
     1201 accagattcg cggcaggaag cggagctact aacttcagcc tgctgaagca ggctggagac
     1261 gtggaggaga accctggacc tactagtatg ccagagccag cgaagtctgc tcccgccccg
     1321 aaaaagggct ccaagaaggc ggtgactaag gcgcagaaga aaggcggcaa gaagcgcaag
     1381 cgcagccgca aggagagcta ttccatctat gtgtacaagg ttctgaagca ggtccaccct
     1441 gacaccggca tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa cgacattttc
     1501 gagcgcatcg caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc
     1561 tccagggaga tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc
     1621 gtgtccgagg gtactaaggc catcaccaag tacaccagcg ctaaggcact cgagatggtg
     1681 tctaagggcg aagagctgat caaggaaaat atgcgtatga aggtggtcat ggaaggttcg
     1741 gtcaacggcc accaattcaa atgcacaggt gaaggagaag gcagaccgta cgagggagtg
     1801 caaaccatga ggatcaaagt catcgaggga ggacccctgc catttgcctt tgacattctt
     1861 gccacgtcgt tcatgtatgg cagccgtacc tttatcaagt acccggccga catccctgat
     1921 ttctttaaac agtcctttcc tgagggtttt acttgggaaa gagttacgag atacgaagat
     1981 ggtggagtcg tcaccgtcac gcaggacacc agccttgagg atggcgagct cgtctacaac
     2041 gtcaaggtca gaggggtaaa ctttccctcc aatggtcccg tgatgcagaa gaagaccaag
     2101 ggttgggagc ctaatacaga gatgatgtat ccagcagatg gtggtctgag aggatacact
     2161 gacatcgcac tgaaagttga tggtggtggc catctgcact gcaacttcgt gacaacttac
     2221 aggtcaaaaa agaccgtcgg gaacatcaag atgcccggtg tccatgccgt tgatcaccgc
     2281 ctggaaagga tcgaggagag tgacaatgaa acctacgtag tgcaaagaga agtggcagtt
     2341 gccaaataca gcaaccttgg tggtggcatg gacgagctgt acaagtga
//
