LOCUS       sequence-211252-        2875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Anion channelrhodopsin GtACR1 fused to ER export signal and targeted
            to the neuronal soma and proximal dendrites under the control of 
            internal ribosome entry sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Mardinly AR, Oldenburg IA, Pegard NC, Sridharan S, Lyall EH, Chesnov
            K, Brohawn SG, Waller L, Adesnik H
  TITLE     Precise multimodal optical control of neural ensemble activity.
  JOURNAL   Nat Neurosci. 2018 Apr 30. pii: 10.1038/s41593-018-0139-8. doi: 
            10.1038/s41593-018-0139-8.
  PUBMED    29713079
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2018 Apr 30. pii: 10.1038/s41593-018-0139-8. doi: 
            10.1038/s41593-018-0139-8."
COMMENT     Sequence Label: pCAG-H2B-mRuby3-IRES-eGtACR1-ST
FEATURES             Location/Qualifiers
     source          1..2875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..378
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             388..1098
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     misc_feature    1133..1705
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1156..1707
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1300..1317)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1527..1546
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2633..2656
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgccagagc cagcgaagtc tgctcccgcc ccgaaaaagg gctccaagaa ggcggtgact
       61 aaggcgcaga agaaaggcgg caagaagcgc aagcgcagcc gcaaggagag ctattccatc
      121 tatgtgtaca aggttctgaa gcaggtccac cctgacaccg gcatttcgtc caaggccatg
      181 ggcatcatga attcgtttgt gaacgacatt ttcgagcgca tcgcaggtga ggcttcccgc
      241 ctggcgcatt acaacaagcg ctcgaccatc acctccaggg agatccagac ggccgtgcgc
      301 ctgctgctgc ctggggagtt ggccaagcac gccgtgtccg agggtactaa ggccatcacc
      361 aagtacacca gcgctaaggc ggccgctatg gtgtctaagg gcgaagagct gatcaaggaa
      421 aatatgcgta tgaaggtggt catggaaggt tcggtcaacg gccaccaatt caaatgcaca
      481 ggtgaaggag aaggcagacc gtacgaggga gtgcaaacca tgaggatcaa agtcatcgag
      541 ggaggacccc tgccatttgc ctttgacatt cttgccacgt cgttcatgta tggcagccgt
      601 acctttatca agtacccggc cgacatccct gatttcttta aacagtcctt tcctgagggt
      661 tttacttggg aaagagttac gagatacgaa gatggtggag tcgtcaccgt cacgcaggac
      721 accagccttg aggatggcga gctcgtctac aacgtcaagg tcagaggggt aaactttccc
      781 tccaatggtc ccgtgatgca gaagaagacc aagggttggg agcctaatac agagatgatg
      841 tatccagcag atggtggtct gagaggatac actgacatcg cactgaaagt tgatggtggt
      901 ggccatctgc actgcaactt cgtgacaact tacaggtcaa aaaagaccgt cgggaacatc
      961 aagatgcccg gtgtccatgc cgttgatcac cgcctggaaa ggatcgagga gagtgacaat
     1021 gaaacctacg tagtgcaaag agaagtggca gttgccaaat acagcaacct tggtggtggc
     1081 atggacgagc tgtacaagtg aattcctcga ctagaattgg gcccgggatc cgcccctctc
     1141 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     1201 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     1261 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     1321 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     1381 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     1441 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     1501 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     1561 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tacacatgct
     1621 ttacatgtgt ttagtcgagg ttaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     1681 tttcctttga aaaacacgat gataatatgg ccacagcttg tccaccatgc tcgagtccat
     1741 gagcagcatc acctgtgatc ccgccatcta cggcgaatgg agcagggaga accagttctg
     1801 cgtggagaag agcctgatca ccctggacgg catcaagtac gtccagctgg tgatggccgt
     1861 cgtgagcgcc tgtcaggtgt tcttcatggt gaccagagcc cccaaggtgc cctgggaagc
     1921 catctacctg cccaccaccg agatgatcac ctattccctg gccttcaccg gaaacggcta
     1981 catcagagtg gccaatggca agtacctgcc ctgggccaga atggccagct ggctgtgcac
     2041 ctgccctatc atgctgggcc tggtgagcaa tatggccctc gtgaagtaca agagcatccc
     2101 cctcaaccct atgatgatcg ccgcctccag catctgcacc gtgttcggca tcaccgcctc
     2161 cgtggtgcta gacccgctgc acgtgtggct gtactgtttc atcagcagca tcttcttcat
     2221 cttcgagatg gtggtggcct tcgccatttt cgccattacc atccacgatt tccagaccat
     2281 cggctccccc atgtccctga aggtggtgga gaggctgaag ctgatgagga tcgtgttcta
     2341 cgtgagctgg atggcctacc ctatcctgtg gagcttctcc agcaccggcg cctgcatcat
     2401 gagcgagaac accagcagcg tgctgtacct gctgggcgac gctctgtgca agaacaccta
     2461 cggcatcctg ctgtgggcta caacctgggg cctgctgaac ggcaagtggg acagggatta
     2521 cgtgaagggc aggaacgtgg acggcaccct gatgcctgag tacgagcagg acctggagaa
     2581 gggcaacacc gagaggtacg aggacgccag agccggcgag accgcaccgg tagattacaa
     2641 agacgatgac gataagcagt cccagcccat cctcaacacc aaggagatgg ccccgcagag
     2701 caagcctcca gaggagctgg agatgagcag catgcccagc cccgtggccc ctctgcccgc
     2761 acgcacggag ggcgtcatcg acatgcggag catgtccagc attgacagct tcatcagctg
     2821 tgccacggac ttccctgaag ccaccagatt cttctgctac gagaacgagg tgtga
//
