LOCUS       sequence-211253-        2518 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Anion channelrhodopsin GtACR1 fused to ER export signal and targeted
            to the neuronal soma and proximal dendrites under the control of 
            internal ribosome entry sequence in a viral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Mardinly AR, Oldenburg IA, Pegard NC, Sridharan S, Lyall EH, Chesnov
            K, Brohawn SG, Waller L, Adesnik H
  TITLE     Precise multimodal optical control of neural ensemble activity.
  JOURNAL   Nat Neurosci. 2018 Apr 30. pii: 10.1038/s41593-018-0139-8. doi: 
            10.1038/s41593-018-0139-8.
  PUBMED    29713079
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2018 Apr 30. pii: 10.1038/s41593-018-0139-8. doi: 
            10.1038/s41593-018-0139-8."
COMMENT     Sequence Label: pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST
FEATURES             Location/Qualifiers
     source          1..2518
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..21
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             31..741
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     misc_feature    776..1348
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    799..1350
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(943..960)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1170..1189
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2276..2299
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cccaagaaga agaggaaggt ggcggccgct atggtgtcta agggcgaaga gctgatcaag
       61 gaaaatatgc gtatgaaggt ggtcatggaa ggttcggtca acggccacca attcaaatgc
      121 acaggtgaag gagaaggcag accgtacgag ggagtgcaaa ccatgaggat caaagtcatc
      181 gagggaggac ccctgccatt tgcctttgac attcttgcca cgtcgttcat gtatggcagc
      241 cgtaccttta tcaagtaccc ggccgacatc cctgatttct ttaaacagtc ctttcctgag
      301 ggttttactt gggaaagagt tacgagatac gaagatggtg gagtcgtcac cgtcacgcag
      361 gacaccagcc ttgaggatgg cgagctcgtc tacaacgtca aggtcagagg ggtaaacttt
      421 ccctccaatg gtcccgtgat gcagaagaag accaagggtt gggagcctaa tacagagatg
      481 atgtatccag cagatggtgg tctgagagga tacactgaca tcgcactgaa agttgatggt
      541 ggtggccatc tgcactgcaa cttcgtgaca acttacaggt caaaaaagac cgtcgggaac
      601 atcaagatgc ccggtgtcca tgccgttgat caccgcctgg aaaggatcga ggagagtgac
      661 aatgaaacct acgtagtgca aagagaagtg gcagttgcca aatacagcaa ccttggtggt
      721 ggcatggacg agctgtacaa gtgaattcct cgactagaat tgggcccggg atccgcccct
      781 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
      841 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
      901 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
      961 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     1021 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     1081 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     1141 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     1201 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtacacat
     1261 gctttacatg tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc acggggacgt
     1321 ggttttcctt tgaaaaacac gatgataata tggccacagc ttgtccacca tgctcgagtc
     1381 catgagcagc atcacctgtg atcccgccat ctacggcgaa tggagcaggg agaaccagtt
     1441 ctgcgtggag aagagcctga tcaccctgga cggcatcaag tacgtccagc tggtgatggc
     1501 cgtcgtgagc gcctgtcagg tgttcttcat ggtgaccaga gcccccaagg tgccctggga
     1561 agccatctac ctgcccacca ccgagatgat cacctattcc ctggccttca ccggaaacgg
     1621 ctacatcaga gtggccaatg gcaagtacct gccctgggcc agaatggcca gctggctgtg
     1681 cacctgccct atcatgctgg gcctggtgag caatatggcc ctcgtgaagt acaagagcat
     1741 ccccctcaac cctatgatga tcgccgcctc cagcatctgc accgtgttcg gcatcaccgc
     1801 ctccgtggtg ctagacccgc tgcacgtgtg gctgtactgt ttcatcagca gcatcttctt
     1861 catcttcgag atggtggtgg ccttcgccat tttcgccatt accatccacg atttccagac
     1921 catcggctcc cccatgtccc tgaaggtggt ggagaggctg aagctgatga ggatcgtgtt
     1981 ctacgtgagc tggatggcct accctatcct gtggagcttc tccagcaccg gcgcctgcat
     2041 catgagcgag aacaccagca gcgtgctgta cctgctgggc gacgctctgt gcaagaacac
     2101 ctacggcatc ctgctgtggg ctacaacctg gggcctgctg aacggcaagt gggacaggga
     2161 ttacgtgaag ggcaggaacg tggacggcac cctgatgcct gagtacgagc aggacctgga
     2221 gaagggcaac accgagaggt acgaggacgc cagagccggc gagaccgcac cggtagatta
     2281 caaagacgat gacgataagc agtcccagcc catcctcaac accaaggaga tggccccgca
     2341 gagcaagcct ccagaggagc tggagatgag cagcatgccc agccccgtgg cccctctgcc
     2401 cgcacgcacg gagggcgtca tcgacatgcg gagcatgtcc agcattgaca gcttcatcag
     2461 ctgtgccacg gacttccctg aagccaccag attcttctgc tacgagaacg aggtgtga
//
