LOCUS       sequence-211255-        8946 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_01g00945 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8946)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 8946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_01g00945-bio-His
FEATURES             Location/Qualifiers
     source          1..8946
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4110..4127
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4179..4234
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4233..4252)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6470..6488)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6556..6660
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6661..6729,6730..7521)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6879..6898)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7692..8280
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8181..8200
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8434..8451
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8568..8589
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8604..8609,8610..8627,8628..8634)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8642..8658
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8647..8669
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8666..8682
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8666..8682
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcct
     1201 ctgagtctga tctgggcggc ctgctgagcg tgcaccaggt cacaaacgcc gagaacctgt
     1261 acgagctggg catcaagcag ctggacagca gcatcaagct gctcgaggac gccaagttca
     1321 ccggcaaccg gatcctgcgg gactacgagc agaccgtgca gtacgtggcc gcccacatcg
     1381 gccccagcgg cagcttcatc aagttcagcc ccgtgatcaa gagactgatc agccagatca
     1441 acaccctgac ccacggcctg aacgacgccc tgagccacgt ggacccattt ctgagcaagc
     1501 tgatgaacga gaagtcccag ctggtggcca acaagaacgt gatctcccag ggcgccgaag
     1561 agaatatcgc caagctgagc acccagagcg cccggaacct gctgatctcc atcaagaagt
     1621 ccctgcagac cctggaactg ggcgagctga acagccggaa gctgaacacc attgccgaga
     1681 agatcggcac cgacgtgcgg cacgccatca agagcaccaa cagcgccctg aacatcctgc
     1741 tgggctacag agtgaccttc accctggtca tcgacagcga gaacatcctg agcgactccg
     1801 agaagttcaa gatcaacatc ttcagccaca gcaagctgtg ccacggcacc accaacgagg
     1861 tggtcaacct gcagcacaag gccaagatgc tgttccagaa gtctctgagc gagggcctgc
     1921 tgcacgagga catcaacatg ggcaagaact acatcatccg gatcgacagc tacgcccacg
     1981 agagcaacaa gctggccagc gagatgaacg ccaagaacta cgccatggcc agcagcgtgc
     2041 ggttcatccg gaaggacatc gacaagctga agcggatcct ggacgactac aagaacagcc
     2101 cccacagcgc ctacaagcgg atggacgtga tccacgtgga aatcgacaag atctacgaga
     2161 gcatcggcac cgccctgaag tgggccaacg ccgccgtgaa gtccaacttc gccatcctga
     2221 ccgccaccag agagacatac caggccctgg aaaacctgct gaagatcagc aagaagtctc
     2281 acagcatcag aagcacctac accacccagc tggacgccga cagcaacagc ggccccttct
     2341 acgccagcac caccgccatc ggcgacaagc acgaccccga cgacctgtac ggcctgaagc
     2401 acagcggcta caacaccgcc aacaacgacg ccagcagccg gtacagctac tacgtgaaga
     2461 agttcggcgg ccacggcagc agaagcaacc acgccacaag cgagagcggc gaggacgact
     2521 gcctgaacga taagtgctgc aagaactgcg agaagtccaa gccctggtac aagagattcg
     2581 gcctgggcag cgtgatcagc ggcatccaga acatgttcgg cgccagctac cagcaggacc
     2641 acagcgacga cgaccactac cccaagttcc cccacagccc ttacatcatc cccaccgccc
     2701 ctcccatcga cctggtgccc aacgacatcc acaccaacac cagaagcaac atcatcaaga
     2761 acggcgtgac cgagaaagtg atccccatga gcagccccaa ctccgcccag agcgaccacg
     2821 agaagaaaga gcccaccagc gacgacaaca agaatgaccc cgtggacacc aatagcgcca
     2881 agatcgagga cgtggccgag acaaagagca gcatcgagac agagacagtg tccaacgacc
     2941 tggaaaacaa ggccgccgaa accgaggaat ccatcgacac cgagccctac gagagcgaga
     3001 gcgtgggcac caagagcagc accggcatcg acaacgatgc caccgtggac cagaacagcg
     3061 gcaacggcca caacgccatt aagaccacaa acaaccccca catccagagc gtgaaccccg
     3121 acttccagcc cgagaacccc aacaaccctc acgtgaacca ctacggcagc ttcgagatcc
     3181 aggccaacat cttcggaaac cagggcaatc tggaaaacat ccagcggctg cagcaggacc
     3241 ccaagatcaa cgagctgatc atcaagggca aagagttctt cgagctgttc aacgccgtga
     3301 agatgaagct gatgcggggc agcagctacc tgaacagcaa gatggtgtcc atcatgctgg
     3361 ccaagatctt ccagggcagc ctgagcaacc tgtgctacaa cttcaaccag aaccggtact
     3421 actacgacac ccaggcctac aatagcgccc tgagcaatgg cgcgccgtcg acctccatca
     3481 cggcctataa gagtgagggg gagtcagcgg agttctcctt cccactcaac cttggagagg
     3541 aaagcctgca gggagagttg agatggaagg cagagaaggc tccttcttcc cagtcctgga
     3601 tcaccttctc cctaaagaac caaaaggtgt ctgtgcagaa gtctactagc aaccccaagt
     3661 tccagctgtc cgaaacgctc ccactcaccc ttcagatacc ccaggtctcc cttcagtttg
     3721 ctggttctgg caacctgacc ctgactctgg acagagggat actgtatcag gaagtgaacc
     3781 tggtggtgat gaaagtgact cagcccgaca gcaacacttt gacctgtgag gtgatgggac
     3841 ccacctcacc caagatgaga ctgatcttga agcaggagaa tcaggaggcc agggtctcca
     3901 ggcaggagaa agtgattcaa gtgcaggccc ctgaagcagg ggtgtggcaa tgtctactga
     3961 gtgaaggtga agaggtcaag atggactcca agatccaggt tttatccaaa gggttgaatt
     4021 ccggcagcct gcaccacatc ctggacgccc agaagatgct gtggaaccac agggacagga
     4081 acctgcctcc tctggcccct ctgggccccc accaccatca ccaccattga gtctagacac
     4141 gtgattaatt aaggatcccc cgacctcgac ctctggctaa taaaggaaat ttattttcat
     4201 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     4261 tcatttggtc gagatccctc ggagatctct agctagagga tcgatccccg ccccggacga
     4321 actaaacctg actacgacat ctctgcccct tcttcgcggg gcagtgcatg taatcccttc
     4381 agttggttgg tacaacttgc caactgggcc ctgttccaca tgtgacacgg ggggggacca
     4441 aacacaaagg ggttctctga ctgtagttga catccttata aatggatgtg cacatttgcc
     4501 aacactgagt ggctttcatc ctggagcaga ctttgcagtc tgtggactgc aacacaacat
     4561 tgcctttatg tgtaactctt ggctgaagct cttacaccaa tgctggggga catgtacctc
     4621 ccaggggccc aggaagacta cgggaggcta caccaacgtc aatcagaggg gcctgtgtag
     4681 ctaccgataa gcggaccctc aagagggcat tagcaatagt gtttataagg cccccttgtt
     4741 aaccctaaac gggtagcata tgcttcccgg gtagtagtat atactatcca gactaaccct
     4801 aattcaatag catatgttac ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa
     4861 gggtcctaag gaacagcgat atctcccacc ccatgagctg tcacggtttt atttacatgg
     4921 ggtcaggatt ccacgagggt agtgaaccat tttagtcaca agggcagtgg ctgaagatca
     4981 aggagcgggc agtgaactct cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc
     5041 taatagaata actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg
     5101 tttcaggtga cgcccccaga ataaaatttg gacggggggt tcagtggtgg cattgtgcta
     5161 tgacaccaat ataaccctca caaacccctt gggcaataaa tactagtgta ggaatgaaac
     5221 attctgaata tctttaacaa tagaaatcca tggggtgggg acaagccgta aagactggat
     5281 gtccatctca cacgaattta tggctatggg caacacataa tcctagtgca atatgatact
     5341 ggggttatta agatgtgtcc caggcaggga ccaagacagg tgaaccatgt tgttacactc
     5401 tatttgtaac aaggggaaag agagtggacg ccgacagcag cggactccac tggttgtctc
     5461 taacaccccc gaaaattaaa cggggctcca cgccaatggg gcccataaac aaagacaagt
     5521 ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca cacgccgccc
     5581 tgcggttttg gactgtaaaa taagggtgta ataacttggc tgattgtaac cccgctaacc
     5641 actgcggtca aaccacttgc ccacaaaacc actaatggca ccccggggaa tacctgcata
     5701 agtaggtggg cgggccaaga taggggcgcg attgctgcga tctggaggac aaattacaca
     5761 cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc atattcacga ggtcgctgag
     5821 agcacggtgg gctaatgttg ccatgggtag catatactac ccaaatatct ggatagcata
     5881 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
     5941 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
     6001 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     6061 tgctatccta atctgtatcc gggtagcata tgctatccta atagagatta gggtagtata
     6121 tgctatccta atttatatct gggtagcata tactacccaa atatctggat agcatatgct
     6181 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agcataggct
     6241 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     6301 atcctaattt atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     6361 atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct
     6421 atcctcatga taagctgtca aacatgagaa ttttcttgaa gacgaaaggg cctcgtgata
     6481 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     6541 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     6601 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     6661 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     6721 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     6781 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     6841 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     6901 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     6961 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     7021 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     7081 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     7141 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     7201 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     7261 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     7321 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     7381 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     7441 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     7501 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     7561 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     7621 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     7681 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     7741 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     7801 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     7861 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     7921 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     7981 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     8041 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     8101 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     8161 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     8221 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     8281 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     8341 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     8401 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     8461 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     8521 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     8581 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     8641 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
     8701 tagctagagg tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt
     8761 tgttgccatt gctgcaggcg cagaactggt aggtatggaa gatctataca ttgaatcaat
     8821 attggcaatt agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc
     8881 cattgcatac gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat
     8941 gaccgc
//
