LOCUS       sequence-211256-       13797 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_04g07535 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13797)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 13797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_04g07535-bio-His
FEATURES             Location/Qualifiers
     source          1..13797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8961..8978
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    9030..9085
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9084..9103)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(11321..11339)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11407..11511
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11512..11580,11581..12372)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11730..11749)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12543..13131
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13032..13051
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13285..13302
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13419..13440
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13455..13460,13461..13478,13479..13485)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13493..13509
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13498..13520
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13517..13533
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13517..13533
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 ctaataccag aggcgccacc ggcaccggcg acaacagaga aggcgtgatc agcagcatca
     1261 ccaccagcgc cgtgaccacc acctccggca ccaccaccac aaccctgagc agcaacaatg
     1321 gcgcccctac caccatcagc cccagcctgg acaccaccca gaacgccgct gccaccaaga
     1381 tcgtgaacct gaacatcaac gagatcggct tcgtggacca ggtgcccgag gacctgtcca
     1441 gcatctacgt gttcagcacc gacggcatct tcaccaaagt gacccccgcc accggcttca
     1501 gcatcggctg cgtgatcttc ggcaaccagc tgatccccca cagcatggac gtgatcaccc
     1561 ggaccgtgtc ctacaccaac aagtaccccc tgatcgtcgt gcggatccag gacaagacca
     1621 gcaactacgc cagcaccgtg tactacgagc agagcggcct gcagagcaac agattcgtgc
     1681 tgcgggacga ccccggcttc atcatccccc agaaccgggc cagcacctac accctgaacg
     1741 acatcaccta caagaccttc gacatcagca ccgccaacga cgacgagttc ctgaagctga
     1801 gcctgagcga cggcagcgtg ctgtacacaa acaaccccga cagcaagttc tacatcggcg
     1861 aagtgaagat cggcgagatc atcatcccta tcaatatcgc cagccagtac atcatcgtga
     1921 agctgtccag caacggcgag ctgatcgagc tgtacaccac cggctgcttc ggcgagcaca
     1981 tcatcaagaa attcaagaaa gtgggcagcg cctacaacga catctccaag gccttcgaca
     2041 ccgtgccctg gatccccacc cacttcgtga tgttccagaa ggtggacttc agcatcccca
     2101 gcgacctgtt cgagctgaac caccacaata tcctgctgcc caccggcgtg aagcacagca
     2161 tccacatcaa cagcgagaca ggcaacgtgg acagcgtggt gttcttcctg agccccctgg
     2221 ccaagcggat gatgaccttc ggcaacatcc ggttccacaa gatcaacctg ccccccttca
     2281 gcctgggcgt gatccactcc atcaccgtgg aaaaagtgat caactccgag gacttcgacg
     2341 agatccagac ccccctccag gtgtccatca ttgccagcta cggccccagc ggcgactaca
     2401 acagcttcgt gttcaccccc gtggtcaccg ccgacgccca cgtgtcctat aagctggaaa
     2461 ccgacttcaa gctggacgtg gacgtcatca ccaacaccgc cctgaagctg cccacctccg
     2521 tgcccggcct gcactacacc gagacaatct accagggcac cgagctgagc aagttcaaca
     2581 agccccagtg caagctgaac gacctgctga tctccaaggg cagcgagctg cagatcatcc
     2641 acgacggcct gaacaacgcc gccatcatca caaacaaaga agtgaacgcc gacgggaccg
     2701 agctgttctt cttcgacctg ctgcccccca gcgacggcac ccctaccctg cagtccaagt
     2761 tctttagcat cctgaagttc atccccatca tctccaccgg cctgaacgag ctgatgctgg
     2821 aactgctgga aaaccccgcc agcagcagcg ccagctccaa gtacagcggc ctgaccggcc
     2881 agctgaaccg gctgatcacc gtgctggacg gcatcgtgga tagcgccagc agcgtgaaga
     2941 acaccgagac tgcccccgac aacgaggaaa ccagcatcag cagcctgaag tccctgatca
     3001 ccaccatcca gggcaacatt gccatcaccc ggaacaaagt gaccaaggac gacgtgtacc
     3061 tgctgaagaa gtccctgtcc ggcctgacca cccggctgat ctaccacagc aaggtggacg
     3121 gaatcagctt cgatatcacc gagagccgga ccaacgagta caacagcctg gatcggacca
     3181 ccaccagcat ggatgacatt atcgccatgt tcagcaaccc caacatgtac ctggtcaagg
     3241 tggcctacct gcaggccatc gagcacatct ttctggtgtc caccaaatac gaggacatct
     3301 tcgactacac cgtggactgc agcaagtgca aggccaccga cagcggcagc ttcagcgatc
     3361 tgctgctggg caacaaagtg aaagagagcc tgagcttcat cgagggcctg atcagcgaca
     3421 tcaagagcca cagcctgaag gccggcgtga ccggcggcat cagctccagc agcctgttcg
     3481 acgacacctt cgatgagctg aagctggacc agaccaccat ccggtccctg gtggcccccc
     3541 tggaagagat caagaacgag ctggaagcca tcaacagcca ggaaacctcc gacgccaccg
     3601 tgacccccag cacccccaac accaacgtga acatcaagac catcatcagc aagatcaaaa
     3661 agttcctgat gatcagcgag acaatcagca gcaccgccct ggccagactg agcgccgtgc
     3721 tgtctatcct gggcagaggc aacagcatca acgccctgcc cgagcggctg accagcatca
     3781 tcgtggacct gaagtctacc agcgtgcccc aggaagtggt cctgaagaac ggcgtgtaca
     3841 agctgaagga ccagttcaag ctgacccaca agatgatccc cgccttcggc aatgtgcagc
     3901 tgcatatccc cgagaagtcc gccgtggtgc agatcagcat cgtggaacac gagaacgacg
     3961 ccaagatggc cgtgatcaca ctggacgacc actttaagct gacactcgag aaagtgatcc
     4021 tgagcgagac acccaccgtc gtgggcctga cccacaccac acaggacccc ctggacatca
     4081 tctcctccat cttcgtgaag atgaacacag ccgccgacgg cattatcgag ggctacctgg
     4141 acctggatct gaacagcaag atcggcaact tcatcagcgc catcgagctg accgacctga
     4201 ccaacaccgt gaagtccgcc accgtgcggc ctccccagct gaaagtgctg gccctgaagt
     4261 ttggcaatca gctggtggaa gtgaaagaaa ccgcccggac cttcgccacc tttgacgaga
     4321 acctgaatag catcgagatc attaccttca acaaggacgg cacaatgatc agcaagtttt
     4381 tcagccgcga gttcatcggc agcatgaccc ccttcgagcc caacgccccc acctacatcg
     4441 agcacgcctc caccgacacc ctgcccagcc cctggatcac cacacacatg cacaacaagc
     4501 ggctggtgga cttcgaggtg cccttcaccg tgatctttga cgacgagctg atcaactact
     4561 acatcgagaa gctgcctaac ggcgtggcct acagcctgta cggcgaggaa ctgtgcgtgc
     4621 ccaagaactc cggcctgctg ctgaaagaga tcaaccacat cagaaccacc aagctgcgcg
     4681 aggtgttcaa gaagatctac aagagcagca agacctccac cgagatcacc agctgcgccc
     4741 agaaaacaaa caccaacgac ggcaccatca agagcaacct gcaggtcatc atgaaggaaa
     4801 acgaccaccc attccccaag cccatcaacg atggcatgca ccggcgggtg gaatccatcc
     4861 acttctacat cgacaacgtg ctgagacacg acaccgcctt tggccggatc accatcggca
     4921 acctggccct gcccgccttc tccatcggct tcatccacag catcttcatc gagcgcgtga
     4981 ccatgggcga caagagcctg gccagcgtgg gcatcatcac caattacggc ccctccggcg
     5041 attacgagct gctgcggtac atgcagctgg aaaacgacaa gcgggacttc gccctggtgc
     5101 agggccccga gatcacagcc gattacatcg gctctggcct gaccaagcac aagatgctga
     5161 ccatgaaggg cgccagcacc agctctatcg gcttcgagac aaactacaaa gagctgatcc
     5221 tgtccaacgg cttcatgcgg cccaccaaca agattgtgac cctgttctac accgactccg
     5281 agaacgtggc cctgggcaag ctgtacagca tcgagaacgg caagcacggc gtgacctact
     5341 ctatctacgg cgccttcccc atcgaggaaa gcagccccga gagcagcagc cccaacgcca
     5401 gaggcttctc cttcgtgttc tccgtgctga acagcatcta tcacatcaac aagctgttca
     5461 cccgggccta caacgccctg gacgccctga aagtgaaaac cagctgcgtg gacggcgaga
     5521 acaagggctc cgccctgagc aaccctgccg ccgagaagta cctgagcatc ctgtcctcca
     5581 acgtgttcat gcccaccctg agccggctgg gaaagctgtt caacgacgtg atgctgaggg
     5641 acgccagctt caccgagaca gccgtgcgga tgctgccccg gttcaacgcc tttatcgcct
     5701 tcctgaacga ggaaaagagc aaggccggca agaacgccct gaacgaagag gaactgatgc
     5761 ggttcatcag catgaccagc ggctttatcg aggacctgga attcgtgctg gatgagctgt
     5821 tcaagcacag cctgcggatc aacaacgagg ccgccaagac catgctgagc agcctgatcc
     5881 tgaacttccg gtacatcaat cacatccgga acctgatcaa cggcatctac tttgagctga
     5941 acagccccac cagcagcacc acaagcggca ccacccagga aacaagcggc aaggccacac
     6001 gggaagagtc caaagagggc aagagcacca agcccagcag cccccctacc ccctccaccc
     6061 agaccatcct gaagcccaag gccccttacc tgcggggcca catcatccag atcgactgca
     6121 acaacgcctt caagaactac gtgaacgccc tcatcaaaga gttccgggcc cacatcaagc
     6181 agaccaccac aagcacaacc accaccatca caatcggcac caacgccaag gatgtggacg
     6241 aactggtgga aaagttcgcc acaatcgcca gcttcgatga gaagttcaag aacgtgttct
     6301 tcgacaactc cttcatcgac gagaacttca agaccctcga gaacatcatg gacgagagcg
     6361 ataccgtgct gcccagctgc tccgagacac acaccaccaa gaacagcgcc accgacccct
     6421 acaccggcct gtccaagctg atccagaaga tcaacgacgc cattatccgg cccatgaccc
     6481 tgcggctgtt caagaatagc ttccccgagc tgtgcaagct gtttgtgaag atgcccgacg
     6541 tggactccaa catcttcatg gatctggatg tggacatcac caataccatc ccatctcgga
     6601 gagtgatgta cagcgacggc cgcttcacca tcatcaatag cctgcccacc ttccggtata
     6661 ccctgaagcc tatcgcccca ggccacgacc tgagcctgtt cagccagctg cccatctcca
     6721 tgatcacagt gaccagcagc caggaacagg ccctgacctc ctgcgtgaac cacggcaacg
     6781 agttcaccac cgtgtccaca gccggcaaga ccacctacac caccagaagc gagctgctgt
     6841 ccctgttcaa gctgtccgcc gagacactgc gggacctgaa cggcgccaag ttcgccctgg
     6901 gcgacatcag cgacagcgcc aacaaggcca aggccctgga agcctacaat agcgccatca
     6961 ccacaatgaa gtccatcagc gccgagctgg aaaagatctt cgatatcctg aagtccacac
     7021 ccaagatcac cttcgagagc atcgtgtcta agctgaccag cgtgaacacc gtggataccg
     7081 ccaacgccga tgtgatcaac gccatgttcg acgatctgag caaggccatc agcagctacc
     7141 tgtacagctt catctccatc aagttccccg aggacagcaa ggaccagcag accaacgagg
     7201 gccacgacac cagcgccctg ctgctggaat atcgggccca gctggccagc ctgtcccgga
     7261 tcaagttcct gctggtgctg gaagatctga tcagctctaa caagtacatc agcatcatct
     7321 ctaagagcga gagcagcgat aacgccgccg acagcagcac ccagagcacc agcgatagca
     7381 gcacccccca gaatctggcc ctgatgggca tggtcaacaa cggcaagctg ggcgacagca
     7441 tcagcctgtg caaagagctg gtcaacatcc tggatatcaa caagaagggc agcacctcta
     7501 tcgccaacgg caagtgcctg tacaagggca cagtgctgga ccccagcagc atgagcgaag
     7561 agatccggcg gagcgtgatc aacttcctgg gcatcattct gagcgacatc gacatcggaa
     7621 tcatgaccga gttcagccgg accatgtaca agacaatcat tgccaatatt gccatgatca
     7681 tcctgcagga aatcgtcgag gtcgtcaacc aggcctaccg ggacgacatc ggcagagcca
     7741 acctgaaccc caatatccac aactaccccg cctttctgga acccatcgat atcgacatcc
     7801 tgtatccaca ggtgccagag cacgtgaagc tggtcaatgg cgtgttcaaa ctgaccggca
     7861 acagaaagac cgagctgaaa ctgcggccca aagtgggctc taagtacctg gaagtgtccc
     7921 cccacgtggc cgtggtccag gtgtcagtgc ccgactccaa cggcatcatc aacgtgtacg
     7981 aggacgacta tcacaagatc acagtgcagc agttcgacat ggacggcaac atcatcatgc
     8041 agggcaaggg cgccatctcc gcccaccctt tcgctcagct ggccttcgcc atcgtgtcca
     8101 gcgtgcacga cgaggcccag accaagatcc tgcagtcctc ctccaacggc gacttcttca
     8161 aagagaacat cctggcctac tacgaggacc agatccagca gaacgagaag atggccttca
     8221 agcagcagcg gaccaccaac gatgcctacg ccatctctgc cgtgttcgcc cactacacag
     8281 actggttcca gggcgagaac tacatccatg gcgcgccgtc gacctccatc acggcctata
     8341 agagtgaggg ggagtcagcg gagttctcct tcccactcaa ccttggagag gaaagcctgc
     8401 agggagagtt gagatggaag gcagagaagg ctccttcttc ccagtcctgg atcaccttct
     8461 ccctaaagaa ccaaaaggtg tctgtgcaga agtctactag caaccccaag ttccagctgt
     8521 ccgaaacgct cccactcacc cttcagatac cccaggtctc ccttcagttt gctggttctg
     8581 gcaacctgac cctgactctg gacagaggga tactgtatca ggaagtgaac ctggtggtga
     8641 tgaaagtgac tcagcccgac agcaacactt tgacctgtga ggtgatggga cccacctcac
     8701 ccaagatgag actgatcttg aagcaggaga atcaggaggc cagggtctcc aggcaggaga
     8761 aagtgattca agtgcaggcc cctgaagcag gggtgtggca atgtctactg agtgaaggtg
     8821 aagaggtcaa gatggactcc aagatccagg ttttatccaa agggttgaat tccggcagcc
     8881 tgcaccacat cctggacgcc cagaagatgc tgtggaacca cagggacagg aacctgcctc
     8941 ctctggcccc tctgggcccc caccaccatc accaccattg agtctagaca cgtgattaat
     9001 taaggatccc ccgacctcga cctctggcta ataaaggaaa tttattttca ttgcaatagt
     9061 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttggt
     9121 cgagatccct cggagatctc tagctagagg atcgatcccc gccccggacg aactaaacct
     9181 gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt cagttggttg
     9241 gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc aaacacaaag
     9301 gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc caacactgag
     9361 tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca ttgcctttat
     9421 gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct cccaggggcc
     9481 caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta gctaccgata
     9541 agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt taaccctaaa
     9601 cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc taattcaata
     9661 gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa agggtcctaa
     9721 ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg gggtcaggat
     9781 tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc aaggagcggg
     9841 cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag ctaatagaat
     9901 aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag gtttcaggtg
     9961 acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct atgacaccaa
    10021 tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa cattctgaat
    10081 atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga tgtccatctc
    10141 acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac tggggttatt
    10201 aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact ctatttgtaa
    10261 caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct ctaacacccc
    10321 cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag tggccactct
    10381 tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc ctgcggtttt
    10441 ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac cactgcggtc
    10501 aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat aagtaggtgg
    10561 gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac acacttgcgc
    10621 ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga gagcacggtg
    10681 ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat atgctatcct
    10741 aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
    10801 aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct
    10861 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
    10921 aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat atgctatcct
    10981 aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc
    11041 tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc
    11101 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt
    11161 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
    11221 tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctcatg
    11281 ataagctgtc aaacatgaga attttcttga agacgaaagg gcctcgtgat acgcctattt
    11341 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
    11401 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
    11461 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
    11521 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
    11581 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
    11641 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
    11701 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
    11761 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
    11821 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
    11881 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
    11941 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
    12001 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
    12061 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
    12121 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
    12181 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
    12241 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
    12301 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
    12361 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
    12421 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    12481 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    12541 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    12601 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    12661 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
    12721 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    12781 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    12841 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    12901 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    12961 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    13021 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    13081 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    13141 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
    13201 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
    13261 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
    13321 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
    13381 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
    13441 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
    13501 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct ctagctagag
    13561 gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat
    13621 tgctgcaggc gcagaactgg taggtatgga agatctatac attgaatcaa tattggcaat
    13681 tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata
    13741 cgttgtatct atatcataat atgtacattt atattggctc atgtccaata tgaccgc
//
