LOCUS       sequence-211262-       12884 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lenti-viral expression of HA-tagged Depdc5 mutant B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12884)
  AUTHORS   Shen K, Huang RK, Brignole EJ, Condon KJ, Valenstein ML, 
            Chantranupong L, Bomaliyamu A, Choe A, Hong C, Yu Z, Sabatini DM
  TITLE     Architecture of the human GATOR1 and GATOR1-Rag GTPases complexes.
  JOURNAL   Nature. 2018 Apr 5;556(7699):64-69. doi: 10.1038/nature26158. Epub 
            2018 Mar 28.
  PUBMED    29590090
REFERENCE   2  (bases 1 to 12884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature26158"; journalName: "Nature"; date: "2018-04-5- 5"; 
            volume: "556"; issue: "7699"; pages: "64-69"
COMMENT     Sequence Label: [pSK476] pLJC5 HA-Depdc5(B)
FEATURES             Location/Qualifiers
     source          1..12884
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    288..309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(324..329,330..347,348..354)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    362..378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     367..389
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     386..402
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     386..402
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     421..441
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        423..441
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        469..695
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             696..876
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    923..1048
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1541..1774
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1959..2003
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2292..3503
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3340..3359
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3524..3533
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3536..3562
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    8446..8563
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8612..9122
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9041..9059
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9144..9743
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9144..9163)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9640..9660
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9871..10104
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10176..10297
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10213..10232)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10267..10286
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10337..10472
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10492..10511)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10493..10511)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10521..10538)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10521..10537)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10530..10552)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10679..11134
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10766..10785)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10976..10997
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11160..11264
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11265..11333,11334..12125)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11483..11502)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12296..12884
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12785..12804
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      241 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      301 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      361 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
      421 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
      481 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
      541 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
      601 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
      661 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
      721 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
      781 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
      841 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
      901 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
      961 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     1021 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     1081 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     1141 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     1201 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1261 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1321 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1381 gcaagcagca gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     1441 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     1501 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     1561 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     1621 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     1681 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     1741 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     1801 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     1861 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     1921 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     1981 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     2041 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     2101 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     2161 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     2221 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgatcacga gactagcctc
     2281 gagcgggatc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac
     2341 ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac
     2401 gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa
     2461 ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg
     2521 aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg
     2581 tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc
     2641 gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg
     2701 gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt
     2761 ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt
     2821 tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag
     2881 gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag aacccaaggt
     2941 cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct ggggcaccat
     3001 ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc gtctgttgcg
     3061 ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg agcgcgcgcc
     3121 ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg ggccacctgc
     3181 cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc ctagggtagg
     3241 ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg aggcgtcagt
     3301 ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg ttttgaacta
     3361 tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt gaaatgtaat
     3421 catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc
     3481 cgtttttggc ttttttgtta gacgaagcgc tagcgctacc ggtgccacca tgggctaccc
     3541 ctatgatgtg cctgactacg cagatctcaa tggtggtggt ggtgggtcga cgatgagaac
     3601 aacaaaggtc tacaaactcg tcatccacaa gaagggcttt gggggcagtg atgatgagct
     3661 agttgtgaac cccaaagtgt tccctcacat caagcttgga gacattgtag agattgcaca
     3721 ccccaacgat gaatacagcc ctctgctttt gcaggtcaag tctcttaagg aagatttaca
     3781 gaaggaaact atcagtgtgg accagactgt gactcaagtg ttccggctga gaccttatca
     3841 ggatgtctat gttaatgtcg tagaccctaa ggatgtgacc cttgacctag tggaattaac
     3901 ttttaaggat cagtatattg gccgtgggga tatgtggcga ctaaagaaaa gtttggtcag
     3961 cacatgtgcc tatatcaccc agaaggtgga gtttgctggc atcagagcac aggctggtga
     4021 actgtgggtt aagaatgaga aggtcatgtg tggctacatc agtgaagata ccagggtggt
     4081 gtttcgttct acgtcggcta tggtttacat atttattcag atgagctgtg aaatgtggga
     4141 ttttgatatt tatggggatt tgtattttga gaaagctgtg aatggtttcc ttgctgatct
     4201 atttaccaag tggaaggaga agaactgtag tcatgaagtg acagtggtcc tgttttctag
     4261 aactttctat gatgcaaaat ctgttgatga atttcctgaa ataaaccgag cctcaattcg
     4321 acaggatcac aaggggagat tctatgaaga cttttacaaa gtggtggtgc agaatgagag
     4381 aagagaagaa tggacttcac ttctcgtaac cattaaaaaa ctcttcatcc agtatccagt
     4441 gttggtgcga ctggaacagg cagagggctt tcctcaagga gataattcta cctcagcaca
     4501 aggaaactac ctggaggcca tcaatctgtc attcaatgtg tttgataagc actacatcaa
     4561 ccgcaacttt gaccgaactg ggcagatgtc agtggtgatc acgcccgggg tgggtgtctt
     4621 tgaagtggac cgcctactca tgatcctgac caagcagcgg atgatagata atggaattgg
     4681 tgtggatttg gtgtgcatgg gaggcagcgg aagcggcgct gtcccattgt tcaagctcca
     4741 taatcggagt gctccccgtg attctcgtct gggcgatgac tataatatcc ctcactggat
     4801 aaaccacagt ttctacacat ccaaaagcca gctcttttgt aatagtttca ccccacgaat
     4861 aaaactggca ggaaagaagc ccgcctctga gaaagcaaaa aatggccgtg atacatctct
     4921 cgggagtcca aaagaatctg agaacgccct tcccatccaa gtagattatg acgcctatga
     4981 cgctcaagtg ttcaggctgc ccggcccatc ccgggcccag tgcctcacca cctgcagatc
     5041 tgtgcgagag cgagagagtc acagtcgaaa gagtgccagc tcctgtgatg tttcatccag
     5101 cccttcccta ccaagccgca cactgcccac tgaggaagtg aggagccagg cttctgacga
     5161 cagctcccta ggcaagagtg ccaacatcct gatgatccca cacccccacc tgcaccagta
     5221 tgaagtcagc agctccttgg gatacaccag cactcgagat gtcctggaga acatgatgga
     5281 gccaccacag cgagactcca gtgcaccagg gaggtttcac gttggcagtg cagaatccat
     5341 gctgcatgtt cgacctggtg gatacacgcc ccagagagca ctgattaacc ccttcgctcc
     5401 ctctcggatg cccatgaagc ttacgtccaa cagaaggcgc tggatgcaca cttttcctgt
     5461 ggggccatcc ggagaagcca tccagatcca ccaccagacc cgacagaata tggcggagct
     5521 acaaggcagc gggcagaggg atccaactca ctcctctgca gagctgctgg agttagcata
     5581 tcatgaagct gctggaaggc acagcaattc ccgccagcct ggtgacggca tgtccttctt
     5641 gaacttcagt ggaacagagg agctttctgt cggcctgctt agcaacagtg gtgcaggtat
     5701 gaatcctagg acccagaata aggattctct agaggacagt gtttctacct ctccagaccc
     5761 aattctgaca ctgtctgctc cccctgtagt gccaggcttc tgttgcacag ttggagtgga
     5821 ctggaagtct ctcactactc cggcgtgcct cccccttacc accgactact tccctgaccg
     5881 ccagggcctg cagaatgact acacagaggg ctgttatgat ctccttccag aagcagacat
     5941 cgacaggagg gacgaagatg gtgtgcagat gacagcccag caggtatttg aagagtttat
     6001 ttgccaacgt ctcatgcagg gctaccaaat catagtgcag cccaagacac agaaacccaa
     6061 tcctgctgtc ccgcccccgc tgagcagtag cccactctat agccgaggcc ttgtgtcccg
     6121 aaaccgccct gaggaggagg accagtattg gctgagtatg ggcagaacgt tccacaaagt
     6181 gacgctgaag gataagatga tcacagtgac gcgatacctt cccaagtatc cttatgaatc
     6241 tgcccagatc cactacacct acagcctctg tccttcccac tcagactcag agttcgtctc
     6301 ctgctgggtg gagttttccc acgaacggct ggaggagtac aagtggaatt acttagatca
     6361 gtatatctgt tctgccggct ctgaagactt cagcttaatt gagtccctga agttctggag
     6421 gacccgcttc ctgctgctgc cagcctgtgt caccgccacc aagcgcatca cggaggggga
     6481 ggcccactgc gacatctatg gggacaggcc ccgtgcagac gaggacgagt ggcaactcct
     6541 ggatggtttt gtccgctttg tggagggctt gaatcgcatt cgcaggcggc atcgctcgga
     6601 tcgcatgatg cggaaaggga ccgccatgaa aggcttgcag atgactgggc ccatttccac
     6661 gcattctctg gagtcaactg cacccccagt ggggaagaag ggaacctcag ctctctctgc
     6721 cctgttggag atggaggcca gtcagaagtg cctgggagaa cagcaggcag ctgtgcatgg
     6781 tgggaagagc tccgcccagt cagccgagag cagcagcgtt gccatgactc ccacctacat
     6841 ggacagccca cgaaaggacg gggccttctt tatggagttt gtccgcagcc cacgcacagc
     6901 atcgtccgcc ttctaccctc aggtatctgt ggaccaaaca gccactccta tgttggacgg
     6961 caccagtttg ggcatatgca caggccaatc catggacaga ggcaacagcc agacctttgg
     7021 gaactcccag aacataggag aacagggcta ctcctccaca aactccagtg acagcagctc
     7081 tcagcagctg gtggcaagct ccttgacctc atcctctacc ctgacagaga tcctggaagc
     7141 catgaagcac ccctcgacag gagtccagct gctctctgaa cagaagggcc tctcaccgta
     7201 ctgcttcatc agcgcggagg tggtacactg gttggtgaac cacgtggagg ggatccagac
     7261 acaggcgatg gccattgaca tcatgcagaa aatgctggaa gagcagctca tcacacatgc
     7321 atctggcgaa gcctggcgga ccttcatcta cggcttctat ttctacaaga tagtaacgga
     7381 caaagagccc gaccgagtgg ccatgcagca gcccgccacc acctggcaca cagcaggagt
     7441 ggacgacttc gccagcttcc agcgcaagtg gtttgaggtg gcctttgtgg cagaagagct
     7501 cgtgcactct gagattcctg cctttctcct gccctggctg cctagccggc cagcctccta
     7561 tgcaagtagg cacagctcct ttagccgaag ttttggagga cggagccagg cggcagcact
     7621 tttagctgcc actgtcccag agcagaggac tgtgaccctg gatgttgacg tgaacaaccg
     7681 cacagaccgg ctggagtggt gcagctgtta ttaccatggc aacttttctc tgaatgcagc
     7741 ctttgagatc aagctgcact ggatggcggt gaccgcagca gtactcttcg agatggtcca
     7801 aggttggcat cggaaagcca cctcctgtgg cttcttgtta gtcccagttt tggaggggcc
     7861 ttttgcactg cccagttacc tgtatggcga cccccttcgt gcccagctct tcatcccact
     7921 caacatcagc tgcttgctca aggagggcag cgagcacctg tttgatagct ttgaacccga
     7981 aacgtactgg gatcgaatgc acctcttcca ggaagccatt gcacacaggt ttgggtttgt
     8041 acaagataaa tattctgcct ctgcttttaa cttccctgct gagaacaagc ctcagtatat
     8101 ccacgttaca ggaacagtgt ttctgcagct gccctactcc aagcgcaagt tctcagggca
     8161 gcagcggcgg cggcggaact ccaccagctc caccaaccag aacatgttct gcgaggagcg
     8221 ggtcggctac aactgggcct acaacaccat gctcaccaaa acatggcgct ccagcgccac
     8281 aggggatgaa aagtttgctg atcggctgct gaaggacttc acggacttct gcatcaaccg
     8341 tgacaaccgg ctggtcacgt tctggacaag ttgcctggag aagatgcatg ccagtgcccc
     8401 gtgagaattc tcgacctcga gacaaatggc agtattcatc cacaatttta aaagaaaagg
     8461 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     8521 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     8581 cagcagagat ccactttggc cgcggctcga gggggttggg gttgcgcctt ttccaaggca
     8641 gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg
     8701 ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg
     8761 ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct
     8821 tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg
     8881 cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata
     8941 gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg
     9001 ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc
     9061 ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc
     9121 agggggatcc accggagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     9181 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     9241 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     9301 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     9361 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     9421 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     9481 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     9541 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     9601 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     9661 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     9721 tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga
     9781 gcgcacgacc ccatgcatcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     9841 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     9901 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     9961 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
    10021 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
    10081 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
    10141 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
    10201 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    10261 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
    10321 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
    10381 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
    10441 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
    10501 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
    10561 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
    10621 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
    10681 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
    10741 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
    10801 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
    10861 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
    10921 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
    10981 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
    11041 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
    11101 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
    11161 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    11221 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
    11281 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
    11341 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
    11401 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
    11461 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
    11521 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    11581 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    11641 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    11701 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    11761 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    11821 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    11881 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    11941 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    12001 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    12061 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    12121 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    12181 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    12241 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    12301 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    12361 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    12421 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    12481 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    12541 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    12601 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    12661 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    12721 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    12781 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    12841 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaa
//
