LOCUS       sequence-211267-        3117 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phaeodactylum U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Graff van Creveld S, Ben-Dor S, Mizrachi A, Alcolombri U, Hopes A, 
            Mock T, Rosenwasser S, Vardi A
  TITLE     Biochemical Characterization of a Novel Redox-Regulated Metacaspase 
            in a Marine Diatom.
  JOURNAL   Front Microbiol. 2021 Sep 8;12:688199. doi: 
            10.3389/fmicb.2021.688199. eCollection 2021.
  PUBMED    34566902
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2021-09-8"; volume: "12:688199. doi"; pages: " 
            10.3389/fmicb.2021.688199. eCollection 2021"
COMMENT     Sequence Label: pCR8/GW:PtU6
FEATURES             Location/Qualifiers
     source          1..3117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     210..226
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     210..226
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     231..250
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        231..249
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    267..366
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(694..793)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(809..826)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(809..825)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(818..840)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      889..975
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1067..1094
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1253..1270)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1424..2012)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1504..1523)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2105..2896)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gctacttgca ttacagctta cgaaccgaac aggcttatgt ccactgggtt cgtgccttca
       61 tccgtttcca cggtgtgcgt cacccggcaa ccttgggtag cagcgaagtc gaggcatttc
      121 tgtcctggct ggtctagagg aggcatgatg atatattttt atcttgtgca atgtaacatc
      181 agagattttg agacacgggc cagagctgcc aggaaacagc tatgaccatg taatacgact
      241 cactataggg gatatcagct ggatggcaaa taatgatttt attttgactg atagtgacct
      301 gttcgttgca acaaattgat gagcaattat tttttataat gccaactttg tacaagaaag
      361 ctgggtcgaa ttcgcccttt ggtctcagga ggttggctcg gaagttggtg ttgacggtga
      421 gctggaaatt ggttgtcggt cactgctagc gagaagaaaa cggaggacag aaggaagtga
      481 aactcggttc gttctcgaca gcctcactgt caatatgctc attttcaatc cttagcgctt
      541 ttaatgtcga attgacggta aattgaatag gatctataat atctacaagg tactttgaca
      601 cgccaagtat tcattgttag tcaacaatat tttagagctt tataaggtca aaaaacacct
      661 tcaaagtcga gtgagaccta agggcgaatt cggagcctgc ttttttgtac aaagttggca
      721 ttataaaaaa gcattgctca tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
      781 aaatcattat ttggggcccg agcttaagac tggccgtcgt tttacaacgt cgtgactggg
      841 aaaacatcca tgctagcgtt aacgcgagag tagggaactg ccaggcatca aataaaacga
      901 aaggctcagt cggaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
      961 ctgagtagga caaatccgcc gggagcggat ttgaacgttg tgaagcaacg gcccggaggg
     1021 tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ctaagcagaa ggccatcctg
     1081 acggatggcc tttttgcgtt tctacaaact cttcctggct agcggtacgc gtattaattg
     1141 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     1201 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     1261 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1321 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1381 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1441 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1501 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1561 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1621 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1681 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1741 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1801 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1861 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     1921 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     1981 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2041 ctgacgctca gtggaacgac gcgtaactca cgttaaggga ttttggtcat gggtggctcg
     2101 agggttattt gccgactacc ttggtgatct cgcctttcac gtagtggaca aattcttcca
     2161 actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc tgtctagctt
     2221 caagtatgac gggctgatac tgggccggca ggcgctccat tgcccagtcg gcagcgacat
     2281 ccttcggcgc gattttgccg gttactgcgc tgtaccaaat gcgggacaac gtaagcacta
     2341 catttcgctc atcgccagcc cagtcgggcg gcgagttcca tagcgttaag gtttcattta
     2401 gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc gctggaccta
     2461 ccaaggcaac gctatgttct cttgcttttg tcagcaagat agccagatca atgtcgatcg
     2521 tggctggctc gaagatacct gcaagaatgt cattgcgctg ccattctcca aattgcagtt
     2581 cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta
     2641 cagcgcggag aatctcgctc tctccagggg aagccgaagt ttccaaaagg tcgttgatca
     2701 aagctcgccg cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac
     2761 tgtgtggctt caggccgcca tccactgcgg agccgtacaa atgtacggcc agcaacgtcg
     2821 gttcgagatg gcgctcgatg acgccaacta cctctgatag ttgagtcgat acttcggcga
     2881 tcaccgcttc cctcataatg tttaactttg ttttagggcg actgccctgc tgcgtaacat
     2941 cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg
     3001 cccgaggcat agactgtacc ccaaaaaaac agtcataaca agccatgaaa accgccactg
     3061 cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatac
//
