LOCUS       sequence-211299-        3720 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli replicative vector which contains the mycophage integrase 
            gene required for the attP/attB integration. Is co-transformed in 
            trans together with pFLAG_attP, but cannot replicate in 
            mycobacteria, and is thus lost shortly after electroporation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Arnold FM, Hohl M, Remm S, Koliwer-Brandl H, Adenau S, Chusri S, 
            Sander P, Hilbi H, Seeger MA
  TITLE     A uniform cloning platform for mycobacterial genetics and protein 
            production.
  JOURNAL   Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.1038/s41598-018-27687-5.
  PUBMED    29934571
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-06-22"; volume: "8(1):9539. doi"; pages: " 
            10.1038/s41598-018-27687-5"
COMMENT     Sequence Label: pMA_Int
FEATURES             Location/Qualifiers
     source          1..3720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     298..320
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     312..329
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     313..329
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1883..1900)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1993..2581)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2073..2092)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2752..3543,3544..3612))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3375..3394
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3613..3717)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta
       61 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
      121 aaatcaaaag aatagaccga gatagggttg agtggccgct acagggcgct cccattcgcc
      181 attcaggctg cgcaactgtt gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc
      241 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
      301 agtcacgacg ttgtaaaacg acggccagtg agcgcgacgt aatattctag tttgttgcta
      361 gctggcggga acggcaatgc tcctggcggc ggcggggctg gtgcccaggt ctccgcacag
      421 agcggcggtg ctggcgctcg cggccaggcg tggttcttcg cgtactgaca agaaaccccc
      481 ctctttagga ctcagtgtcc ttgggagggg ggctttttgc gtttcaggag gtcttggcca
      541 gcttggacat cgcctcagcg atagcctcgt cgcgggcctc agacgccatc tggtacttca
      601 tcgccatcct aggagtcgtg tgaccgagac gggccatcag ctccttggtc gtcgcacctg
      661 cctgagcggc gaacgtagcg ccgacagcgc ggaggtcgtg gatgcggagt tccggccgac
      721 cgatcttggc gtagccacgc ttcagcgact tggtgaacgc ggacttcgac agccggttgc
      781 cctgcgtcgt ggtcaccagg aatgcctcgg ggcccttgtt catcttcgta cggtccttca
      841 tgtgcgctcg gatcatctcc gcgacgtgag gcggaaccgt cacaggacgc ttcgaccgga
      901 cggtcttggc gttgccaacg acgatcttgt tccccacgcg ggaagcgcca cggcgcaccc
      961 ggagcttcat cgtcatgccg tcgtccacga tgtccttgcg gcgaagctcg atcagctctc
     1021 cgaaccggag gctcgtccac gccaggatgt atgccgcgat ccggtagtgc acgaagatct
     1081 cagcggcgac gatgtccagc tcctcaggcg tcagcgcctc tacgtcgcgc tcatcggctg
     1141 ccttctgctc gatccggcac gggttctctg cgatcagctt gtcctcgacc gctgtgttca
     1201 tcaccgcccg gaggacgttg taggcatgcc ggcgggcagt cgggtgcttc ctacccatcc
     1261 cggcccacca cgcacgcacc agagctggcg tcatctctgt gaccgccact tcacctagca
     1321 ccgggtagat gcggcgctcc gcgtgcccgc tgtacagatc cctggtgccg tctgcgaggt
     1381 cgcgctccac gagccacttc cgggtgtact cctccagcgt gatggcgctg gcggctgcct
     1441 tcttcgcccg gtcctgtgga ggggtccagg tctccatctc gatgagccgc ttctcgcccg
     1501 cgagccaggc ttcggcgtcc atcttgttgt cgtaggtctg cagcgcgtag tacctcacac
     1561 cgtcctgcgg gttgacgtat gaggcttgga tcctcccgct gcgctgagtc ttcagcgatc
     1621 cccatccgcg acgtgccaac taggtctcct ctcgtcgtga acaaggctac cgggttgcaa
     1681 ctcctgtgca actctcaggc ttcaacgcgc ttctacgacc tgcaatttct ttccacttag
     1741 aggatgcagc cgagaggggg taaaaaccta tcttgaccgg cccatatgca tcactgcccg
     1801 ctttccagtc gggaaacctg tcgtgccagc tgcattaaca tggtcatagc tgtttccttg
     1861 cgtattgggc gctctccgct tcctcgctca ctgactcgct gcgctcggtc gttcgggtaa
     1921 agcctggggt gcctaatgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1981 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2041 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2101 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2161 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2221 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2281 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2341 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2401 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2461 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2521 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2581 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2641 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2701 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     2761 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     2821 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     2881 aatgataccg cgagaaccac gctcaccggc tccagattta tcagcaataa accagccagc
     2941 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     3001 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     3061 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3121 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3181 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3241 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3301 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     3361 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     3421 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     3481 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     3541 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     3601 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     3661 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
//
