LOCUS       sequence-211305-        8218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8218)
  AUTHORS   Graff, Mock
  TITLE     Mock lab P. tricornutum plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAGM4723:Ble:Cas9
FEATURES             Location/Qualifiers
     source          1..8218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             269..643
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFIIAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     CDS             1160..5263
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1160..5263
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1160..5263
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5276..5296
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5303..5305,5306..5308,5309..6022)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5348..5369)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5609..5628)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5956..5977
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        6333..6437
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6438..7232
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7403..7991
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7892..7911
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    8127..8151
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gacatacctt cagcgtcgtc tacactgtca cagtcaactg
       61 acagtaatcg ttgatccgga gagattcaaa attcaatctg tttggacctg gataagacac
      121 aagagcgaca tcctgacatg aacgccgtaa acagcaaatc ctggttgaac acgtatcctt
      181 ttgggggcct cccgctacga cgctcgctcc agctggggct tccttactat acacagcgcg
      241 catatttcac ggttgccaga tgtcaagaat ggccaagttg accagtgccg ttccggtgct
      301 caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg
      361 ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcat
      421 cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct
      481 ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg
      541 gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc
      601 ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgagcttcct tccttaaaaa
      661 tttaattttc attagttgca gtcactccgc tttggtttca cagtcaggaa taacactagc
      721 tcgacttcac catggatgcc aatctcgcct attcatggtg tataaaagtt caacatccaa
      781 agctagaact tttggaaaga gaaagaatat ccgaataggg cacggcgtgc cgtattgttg
      841 gagtggacta gcagaaagtg aggaaggcac aggatgagtt ttctcgagcg ctgcaagaat
      901 tcaagcttgg agacatacct tcagcgtcgt ctacactgtc acagtcaact gacagtaatc
      961 gttgatccgg agagattcaa aattcaatct gtttggacct ggataagaca caagagcgac
     1021 atcctgacat gaacgccgta aacagcaaat cctggttgaa cacgtatcct tttgggggcc
     1081 tcccgctacg acgctcgctc cagctggggc ttccttacta tacacagcgc gcatatttca
     1141 cggttgccag atgtcaagaa tggacaagaa gtactccatt gggctcgata tcggcacaaa
     1201 cagcgtcggc tgggccgtca ttacggacga gtacaaggtg ccgagcaaaa aattcaaagt
     1261 tctgggcaat accgatcgcc acagcataaa gaagaacctc attggcgccc tcctgttcga
     1321 ctccggggag acggccgaag ccacgcggct caaaagaaca gcacggcgca gatatacccg
     1381 cagaaagaat cggatctgct acctgcagga gatctttagt aatgagatgg ctaaggtgga
     1441 tgactctttc ttccataggc tggaggagtc ctttttggtg gaggaggata aaaagcacga
     1501 gcgccaccca atctttggca atatcgtgga cgaggtggcg taccatgaaa agtacccaac
     1561 catatatcat ctgaggaaga agcttgtaga cagtactgat aaggctgact tgcggttgat
     1621 ctatctcgcg ctggcgcata tgatcaaatt tcggggacac ttcctcatcg agggggacct
     1681 gaacccagac aacagcgatg tcgacaaact ctttatccaa ctggttcaga cttacaatca
     1741 gcttttcgaa gagaacccga tcaacgcatc cggagttgac gccaaagcaa tcctgagcgc
     1801 taggctgtcc aaatcccggc ggctcgaaaa cctcatcgca cagctccctg gggagaagaa
     1861 gaacggcctg tttggtaatc ttatcgccct gtcactcggg ctgaccccca actttaaatc
     1921 taacttcgac ctggccgaag atgccaagct tcaactgagc aaagacacct acgatgatga
     1981 tctcgacaat ctgctggccc agatcggcga ccagtacgca gacctttttt tggcggcaaa
     2041 gaacctgtca gacgccattc tgctgagtga tattctgcga gtgaacacgg agatcaccaa
     2101 agctccgctg agcgctagta tgatcaagcg ctatgatgag caccaccaag acttgacttt
     2161 gctgaaggcc cttgtcagac agcaactgcc tgagaagtac aaggaaattt tcttcgatca
     2221 gtctaaaaat ggctacgccg gatacattga cggcggagca agccaggagg aattttacaa
     2281 atttattaag cccatcttgg aaaaaatgga cggcaccgag gagctgctgg taaagcttaa
     2341 cagagaagat ctgttgcgca aacagcgcac tttcgacaat ggaagcatcc cccaccagat
     2401 tcacctgggc gaactgcacg ctatcctcag gcggcaagag gatttctacc cctttttgaa
     2461 agataacagg gaaaagattg agaaaatcct cacatttcgg ataccctact atgtaggccc
     2521 cctcgcccgg ggaaattcca gattcgcgtg gatgactcgc aaatcagaag agactatcac
     2581 tccctggaac ttcgaggaag tcgtggataa gggggcctct gcccagtcct tcatcgaaag
     2641 gatgactaac tttgataaaa atctgcctaa cgaaaaggtg cttcctaaac actctctgct
     2701 gtacgagtac ttcacagttt ataacgagct caccaaggtc aaatacgtca cagaagggat
     2761 gagaaagcca gcattcctgt ctggagagca gaagaaagct atcgtggacc tcctcttcaa
     2821 gacgaaccgg aaagttaccg tgaaacagct caaagaagat tatttcaaaa agattgaatg
     2881 tttcgactct gttgaaatca gcggagtgga ggatcgcttc aacgcatccc tgggaacgta
     2941 tcacgatctc ctgaaaatca ttaaagacaa ggacttcctg gacaatgagg agaacgagga
     3001 cattcttgag gacattgtcc tcacccttac gttgtttgaa gatagggaga tgattgaaga
     3061 acgcttgaaa acttacgctc atctcttcga cgacaaagtc atgaaacagc tcaagaggcg
     3121 ccgatataca ggatgggggc ggctgtcaag aaaactgatc aatgggatcc gagacaagca
     3181 gagtggaaag acaatcctgg attttcttaa gtccgatgga tttgccaacc ggaacttcat
     3241 gcagttgatc catgatgact ctctcacctt taaggaggac atccagaaag cacaagtttc
     3301 tggccagggg gacagtctcc acgagcacat cgctaatctt gcaggtagcc cagctatcaa
     3361 aaagggaata ctgcagaccg ttaaggtcgt ggatgaactc gtcaaagtaa tgggaaggca
     3421 taagcccgag aatatcgtta tcgagatggc ccgagagaac caaactaccc agaagggaca
     3481 gaagaacagt agggaaagga tgaagaggat tgaagagggt ataaaagaac tggggtccca
     3541 aatccttaag gaacacccag ttgaaaacac ccagcttcag aatgagaagc tctacctgta
     3601 ctacctgcag aacggcaggg acatgtacgt ggatcaggaa ctggacatca atcggctctc
     3661 cgactacgac gtggatcata tcgtgcccca gtcttttctc aaagatgatt ctattgataa
     3721 taaagtgttg acaagatccg ataaaaatag agggaagagt gataacgtcc cctcagaaga
     3781 agttgtcaag aaaatgaaaa attattggcg gcagctgctg aacgccaaac tgatcacaca
     3841 acggaagttc gataatctga ctaaggctga acgaggtggc ctgtctgagt tggataaagc
     3901 cggcttcatc aaaaggcagc ttgttgagac acgccagatc accaagcacg tggcccaaat
     3961 tctcgattca cgcatgaaca ccaagtacga tgaaaatgac aaactgattc gagaggtgaa
     4021 agttattact ctgaagtcta agctggtttc agatttcaga aaggactttc agttttataa
     4081 ggtgagagag atcaacaatt accaccatgc gcatgatgcc tacctgaatg cagtggtagg
     4141 cactgcactt atcaaaaaat atcccaagct tgaatctgaa tttgtttacg gagactataa
     4201 agtgtacgat gttaggaaaa tgatcgcaaa gtctgagcag gaaataggca aggccaccgc
     4261 taagtacttc ttttacagca atattatgaa ttttttcaag accgagatta cactggccaa
     4321 tggagagatt cggaagcgac cacttatcga aacaaacgga gaaacaggag aaatcgtgtg
     4381 ggacaagggt agggatttcg cgacagtccg gaaggtcctg tccatgccgc aggtgaacat
     4441 cgttaaaaag accgaagtac agaccggagg cttctccaag gaaagtatcc tcccgaaaag
     4501 gaacagcgac aagctgatcg cacgcaaaaa agattgggac cccaagaaat acggcggatt
     4561 cgattctcct acagtcgctt acagtgtact ggttgtggcc aaagtggaga aagggaagtc
     4621 taaaaaactc aaaagcgtca aggaactgct gggcatcaca atcatggagc gatcaagctt
     4681 cgaaaaaaac cccatcgact ttctcgaggc gaaaggatat aaagaggtca aaaaagacct
     4741 catcattaag cttcccaagt actctctctt tgagcttgaa aacggccgga aacgaatgct
     4801 cgctagtgcg ggcgagctgc agaaaggtaa cgagctggca ctgccctcta aatacgttaa
     4861 tttcttgtat ctggccagcc actatgaaaa gctcaaagga tctcccgaag ataatgagca
     4921 gaagcagctg ttcgtggaac aacacaaaca ctaccttgat gagatcatcg agcaaataag
     4981 cgaattctcc aaaagagtga tcctcgccga cgctaacctc gataaggtgc tttctgctta
     5041 caataagcac agggataagc ccatcaggga gcaggcagaa aacattatcc acttgtttac
     5101 tctgaccaac ttgggcgcgc ctgcagcctt caagtacttc gacaccacca tagacagaaa
     5161 gcggtacacc tctacaaagg aggtcctgga cgccacactg attcatcagt caattacggg
     5221 gctctatgaa acaagaatcg acctctctca gctcggtgga gacagcaggg ctgaccccaa
     5281 gaagaagagg aaggtgggtt cgatggtgag caagggcgag gagctgttca ccggggtggt
     5341 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     5401 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     5461 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc
     5521 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     5581 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     5641 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     5701 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     5761 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     5821 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     5881 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa
     5941 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     6001 catggacgag ctgtacaagt gagcttcctt ccttaaaaat ttaattttca ttagttgcag
     6061 tcactccgct ttggtttcac agtcaggaat aacactagct cgacttcacc atggatgcca
     6121 atctcgccta ttcatggtgt ataaaagttc aacatccaaa gctagaactt ttggaaagag
     6181 aaagaatatc cgaatagggc acggcgtgcc gtattgttgg agtggactag cagaaagtga
     6241 ggaaggcaca ggatgagttt tctcgagcgc tactagagga tgcacatgtg accgagggac
     6301 acgaagtgat ccccacatca aggctccgag tgcgcggaac ccctatttgt ttatttttct
     6361 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     6421 attgaaaaag gaagagtatg gctaaaatga gaatatcacc ggaattgaaa aaactgatcg
     6481 aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta tataagctgg
     6541 tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa gggaccacct
     6601 atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg cctgttccaa
     6661 aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagt gaggccgatg
     6721 gcgtcctttg ctcggaagag tatgaagatg aacaaagccc tgaaaagatt atcgagctgt
     6781 atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc tatacgaata
     6841 gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg gccgatgtgg
     6901 attgcgaaaa ctgggaagag gacactccat ttaaagatcc gcgcgagctg tatgattttt
     6961 taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg ggagacagca
     7021 acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga agcggcaggg
     7081 cggacaagtg gtatgacatt gccttctgcg tccggtcgct cagggaggat atcggggaag
     7141 aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg gagaaaataa
     7201 aatattatat tttactggat gaattgtttt agctgtcaga ccaagtttac tcatatatac
     7261 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     7321 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7381 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7441 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7501 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     7561 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7621 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7681 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7741 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7801 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7861 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7921 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7981 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctgctcgga tctgttggac
     8041 cggacagtag tcatggttga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc
     8101 cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg
     8161 cttagacaac ttaataacac attgcggacg tttttaatgt actggggttg aacactct
//
