LOCUS       sequence-211308-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 2 cloning vector, for golden gate cloning for diatoms. All the
            Agrobacterium T-DNA delivery were deleted..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Graff van Creveld S, Coesel SN, Blaskowski S, Groussman RD, Schatz 
            MJ, Armbrust EV
  TITLE     Divergent functions of two clades of flavodoxin in diatoms mitigate 
            oxidative stress and iron limitation.
  JOURNAL   Elife. 2023 Jun 6;12:e84392. doi: 10.7554/eLife.84392.
  PUBMED    37278403
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-06-6"; volume: "12:e84392. doi"; pages: " 
            10.7554/eLife.84392"
COMMENT     Sequence Label: pAGM4723-Del
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..73
                     /gene="bla"
                     /label=AmpR promoter
     CDS             74..868
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
       61 aaaaaggaag agtatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa
      121 ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg
      181 agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga
      241 tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt
      301 cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt
      361 cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc
      421 ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata cgaatagctt
      481 agacagccgc ttagccgaat tggattactt actgaataac gatctggccg atgtggattg
      541 cgaaaactgg gaagaggaca ctccatttaa agatccgcgc gagctgtatg attttttaaa
      601 gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat
      661 ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga
      721 caagtggtat gacattgcct tctgcgtccg gtcgctcagg gaggatatcg gggaagaaca
      781 gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata
      841 ttatatttta ctggatgaat tgttttagct gtcagaccaa gtttactcat atatacttta
      901 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
      961 tctcatgacc aaaatccctt aacgtgag
//
