LOCUS       sequence-211318-        8610 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A single vector mammalian expression system containing a CAG 
            promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 
            LMD-9) and its U6-driven sgRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8610)
  AUTHORS   Agudelo D, Carter S, Velimirovic M, Duringer A, Rivest JF, Levesque 
            S, Loehr J, Mouchiroud M, Cyr D, Waters PJ, Laplante M, Moineau S, 
            Goulet A, Doyon Y
  TITLE     Versatile and robust genome editing with Streptococcus thermophilus 
            CRISPR1-Cas9.
  JOURNAL   Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub
            2020 Jan 3.
  PUBMED    31900288
REFERENCE   2  (bases 1 to 8610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.255414.119"; journalName: "Genome Res"; date: "2020-01";
            volume: "30"; issue: "1"; pages: "107-117"
COMMENT     Sequence Label: U6_sgRNA_CAG_hSt1Cas9_LMD9
FEATURES             Location/Qualifiers
     source          1..8610
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          48..1064
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     988..1010
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1072..1091
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1157..1166
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1172..1192
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1217..4576
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus 
                     thermophilus Type II CRISPR/Cas system (Esvelt et al., 
                     2013)"
                     /label=St1Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="SDLVLGLDIGIGSVGVGILNKVTGEIIHKNSRIFPAAQAENNLVR
                     RTNRQGRRLARRKKHRRVRLNRLFEESGLITDFTKISINLNPYQLRVKGLTDELSNEEL
                     FIALKNMVKHRGISYLDDASDDGNSSVGDYAQIVKENSKQLETKTPGQIQLERYQTYGQ
                     LRGDFTVEKDGKKHRLINVFPTSAYRSEALRILQTQQEFNPQITDEFINRYLEILTGKR
                     KYYHGPGNEKSRTDYGRYRTSGETLDNIFGILIGKCTFYPDEFRAAKASYTAQEFNLLN
                     DLNNLTVPTETKKLSKEQKNQIINYVKNEKAMGPAKLFKYIAKLLSCDVADIKGYRIDK
                     SGKAEIHTFEAYRKMKTLETLDIEQMDRETLDKLAYVLTLNTEREGIQEALEHEFADGS
                     FSQKQVDELVQFRKANSSIFGKGWHNFSVKLMMELIPELYETSEEQMTILTRLGKQKTT
                     SSSNKTKYIDEKLLTEEIYNPVVAKSVRQAIKIVNAAIKEYGDFDNIVIEMARETNEDD
                     EKKAIQKIQKANKDEKDAAMLKAANQYNGKAELPHSVFHGHKQLATKIRLWHQQGERCL
                     YTGKTISIHDLINNSNQFEVDHILPLSITFDDSLANKVLVYATANQEKGQRTPYQALDS
                     MDDAWSFRELKAFVRESKTLSNKKKEYLLTEEDISKFDVRKKFIERNLVDTRYASRVVL
                     NALQEHFRAHKIDTKVSVVRGQFTSQLRRHWGIEKTRDTYHHHAVDALIIAASSQLNLW
                     KKQKNTLVSYSEDQLLDIETGELISDDEYKESVFKAPYQHFVDTLKSKEFEDSILFSYQ
                     VDSKFNRKISDATIYATRQAKVGKDKADETYVLGKIKDIYTQDGYDAFMKIYKKDKSKF
                     LMYRHDPQTFEKVIEPILENYPNKQINEKGKEVPCNPFLKYKEEHGYIRKYSKKGNGPE
                     IKSLKYYDSKLGNHIDITPKDSNNKVVLQSVSPWRADVYFNKTTGKYEILGLKYADLQF
                     EKGTGTYKISQEKYNDIKKKEGVDSDSEFKFTLYKNDLLLVKDTETKEQQLFRFLSRTM
                     PKQKHYVELKPYDKQKFEGGEALIKVLGNVANSGQCKKGLGKSNISIYKVRTDVLGNQH
                     IIKNEGDKPKLDF"
     CDS             4589..4609
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4698..4717)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4763..4818
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4817..4836)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5179..5195)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5179..5195)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5192..5214)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5203..5219
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5227..5233,5234..5251,5252..5257))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5272..5293
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5352..5547
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5398..5533
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5553..5687
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5590..5609)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5644..5663
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5755..5772)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5926..6514)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6006..6025)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6685..7476,7477..7545))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7308..7327
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7546..7650)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7770..8018
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7770..7790
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7941..7960
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        8190..8493
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc
       61 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
      121 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
      181 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
      241 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
      301 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
      361 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
      421 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
      481 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
      541 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
      601 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
      661 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
      721 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
      781 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
      841 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
      901 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
      961 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     1021 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     1081 tggttattgt gctgtctcat cattttggca aagaattctg cagtcgacgg taccgcgggc
     1141 ccgggatcca ccggtcgcca ccatgggcgc cccaaagaag aagcggaagg tcggtatcca
     1201 cggagtccca gcagccagcg acctggtgct gggcctggac atcggcatcg gcagcgtggg
     1261 cgtgggcatc ctgaacaagg tgaccggcga gatcatccac aagaacagtc gcatcttccc
     1321 tgctgctcag gctgagaaca acctggtgcg ccgcaccaac cgccagggtc gccggcttgc
     1381 tcgccgcaag aagcaccggc gcgtgcgcct gaaccgcctg ttcgaggaga gcggcctgat
     1441 caccgacttc accaagatca gcatcaacct gaacccctac cagctgcgcg tgaagggcct
     1501 gaccgacgag ctgagcaacg aggagctgtt catcgccctg aagaacatgg tgaagcaccg
     1561 cggcatcagc tacctggacg acgccagcga cgacggcaac agcagcgtgg gcgactacgc
     1621 ccagatcgtg aaggagaaca gcaagcagct ggagaccaag acccccggcc agatccagct
     1681 ggagcgctac cagacctacg gccagctgcg cggcgacttc accgtggaga aggacggcaa
     1741 gaagcaccgc ctgatcaacg tgttccccac cagcgcctac cgcagcgagg ccctgcgcat
     1801 cctgcagacc cagcaggagt tcaaccccca gatcaccgac gagttcatca accgctacct
     1861 ggagatcctg accggcaagc gcaagtacta ccacggcccc ggcaacgaga agagccgcac
     1921 cgactacggc cgctaccgca ccagcggcga gaccctggac aacatcttcg gcatcctgat
     1981 cggcaagtgc accttctacc ccgacgagtt ccgcgccgcc aaggccagct acaccgccca
     2041 ggagttcaat ctgctgaacg acctgaacaa cctgaccgtg cccaccgaga ccaagaagct
     2101 gagcaaggag cagaagaacc agatcatcaa ctacgtgaag aacgagaagg ctatgggccc
     2161 cgccaagctg ttcaagtaca tcgccaagct gctgagctgc gacgtggccg acatcaaggg
     2221 ctaccgcatc gacaagagcg gcaaggccga gatccacacc ttcgaggcct accgcaagat
     2281 gaagaccctg gagaccctgg acatcgagca gatggaccga gagaccctgg acaagctggc
     2341 ctacgtgctg accctgaaca ccgagcgcga gggcatccag gaggccctgg agcacgagtt
     2401 cgccgacggc agcttcagcc agaaacaggt ggacgagctg gtgcagttcc gcaaggccaa
     2461 cagcagcatc ttcggcaagg gctggcacaa cttcagcgtg aagctgatga tggagctgat
     2521 ccccgagctg tacgagacca gcgaggagca gatgaccatc ctgacccgcc tgggcaagca
     2581 gaagaccacc agcagcagca acaagaccaa gtacatcgac gagaagctgc tgaccgagga
     2641 gatctacaac cccgtggtgg ccaagagcgt gcgccaggcc atcaagatcg tgaacgccgc
     2701 catcaaggag tacggcgact tcgacaacat cgtgatcgag atggcccgcg agaccaacga
     2761 ggacgacgag aagaaggcca tccagaagat ccagaaggcc aacaaggacg agaaggacgc
     2821 cgccatgctg aaggccgcca accagtacaa cggcaaggcc gagctgcccc acagcgtgtt
     2881 ccacggccac aagcagctgg ccaccaagat ccgcctgtgg caccagcagg gcgagcgctg
     2941 cctgtacacc ggcaagacca tcagcatcca cgacctgatc aacaacagca accagttcga
     3001 ggtggaccac atcctgcccc tgagcatcac cttcgacgac agcctggcca acaaggtgct
     3061 ggtgtacgcc accgccaacc aggagaaggg ccagcgcacc ccctaccagg ccctggacag
     3121 catggacgac gcctggagct tccgcgagct gaaggccttc gtgcgcgaga gcaagaccct
     3181 gagcaacaag aagaaggagt atctgctgac cgaggaggac atcagcaagt tcgacgtgcg
     3241 caagaagttc atcgagcgca acctggtgga cacccgctac gccagccgcg tggtgctgaa
     3301 cgccctgcag gagcacttcc gcgcccacaa gatcgacacc aaggtgagcg tggtgcgcgg
     3361 ccagttcacc agccagctgc gccgccactg gggcatcgag aagacccgcg acacctacca
     3421 ccaccacgcc gtggacgccc tgatcattgc ggcttctagc cagctgaacc tgtggaagaa
     3481 gcagaagaac accctggtga gctacagcga ggaccagctg ctggacatcg agaccggcga
     3541 gctgatcagc gacgacgagt acaaggagag cgtgttcaag gccccctacc agcacttcgt
     3601 ggacaccctg aagagcaagg agttcgagga cagcatcctg ttcagctacc aggtggacag
     3661 caagttcaac cgcaagatca gcgacgccac catctacgcc acccgccagg ccaaggtggg
     3721 caaggacaag gccgacgaga cctacgtgct gggcaagatc aaggacatct acacccagga
     3781 cggctacgac gccttcatga agatctacaa gaaggacaag agcaagttcc tgatgtaccg
     3841 ccacgacccc cagaccttcg agaaggtgat cgagcccatc ctggagaact accccaacaa
     3901 gcagatcaac gagaaaggca aggaggtgcc ctgcaacccc ttcctgaagt acaaggagga
     3961 gcacggctac atccgcaagt acagcaagaa gggcaacggc cccgagatca agagcctgaa
     4021 gtactacgac agcaagctgg gcaaccacat cgacatcacc cccaaggaca gcaacaacaa
     4081 ggtggtgctg cagagcgtga gcccctggcg cgccgacgtg tacttcaaca agaccaccgg
     4141 caagtacgag atcctggggc tgaagtacgc cgatctgcag tttgagaaag gcacaggcac
     4201 ctacaagatc agccaggaga agtacaacga catcaagaag aaggagggcg tggacagcga
     4261 cagcgagttc aagttcaccc tgtacaagaa cgaccttctg ctggtgaagg acaccgagac
     4321 caaggagcaa cagctgttcc gcttcctgag ccgcaccatg cccaagcaga agcactacgt
     4381 ggagctgaag ccctacgaca agcagaagtt cgagggcggc gaggccctga tcaaggtgct
     4441 gggcaacgtg gccaacagcg gccagtgcaa gaagggcctg ggcaagagca acatcagcat
     4501 ctacaaggtg cgcaccgacg tgctgggcaa ccagcacatc atcaagaacg agggcgacaa
     4561 gcccaagttg gacttcagca gggctgaccc caagaagaag aggaaggtgt gagcggccgc
     4621 actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct
     4681 cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc
     4741 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
     4801 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag
     4861 aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa
     4921 ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc
     4981 catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt
     5041 tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc
     5101 tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcagccc
     5161 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     5221 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     5281 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     5341 ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     5401 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     5461 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     5521 ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt
     5581 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5641 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg
     5701 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     5761 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     5821 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     5881 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     5941 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6001 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6061 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6121 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6181 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6241 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6301 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6361 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6421 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     6481 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     6541 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     6601 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     6661 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     6721 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     6781 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     6841 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     6901 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     6961 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7021 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7081 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7141 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7201 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7261 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     7321 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     7381 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     7441 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     7501 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     7561 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     7621 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggtc
     7681 gacattgatt attgactagc tgtacaaaaa agcaggcttt aaaggaacca attcagtcga
     7741 ctggatccgg taccaaggtc gggcaggaag agggcctatt tcccatgatt ccttcatatt
     7801 tgcatatacg atacaaggct gttagagaga taattagaat taatttgact gtaaacacaa
     7861 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
     7921 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
     7981 tcttggcttt atatatcttg tggaaaggac gaaacacccg agacgattaa tgcgtctcgg
     8041 tctttgtact ctggtaccag aagctacaaa gataaggctt catgccgaaa tcaacaccct
     8101 gtcattttat ggcagggtgt tttttttact agttattaat agtaatcaat tacggggtca
     8161 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     8221 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     8281 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     8341 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     8401 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     8461 tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc
     8521 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
     8581 ttttgtgcag cgatgggggc gggggggggg
//
