LOCUS       sequence-211322-       18857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  targeting vector to generate a replacement of CDS of M71 with CDS of
            mouse beta2-adrenergic receptor with expression of tauGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18857)
  AUTHORS   Omura M, Grosmaitre X, Ma M, Mombaerts P
  TITLE     The beta2-adrenergic receptor as a surrogate odorant receptor in 
            mouse olfactory sensory neurons.
  JOURNAL   Mol Cell Neurosci. 2014 Jan;58:1-10. doi: 10.1016/j.mcn.2013.10.010.
            Epub 2013 Nov 6.
  PUBMED    24211702
REFERENCE   2  (bases 1 to 18857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2014-01"; volume: "58:1-10. doi"; pages: " 
            10.1016/j.mcn.2013.10.010. Epub 2013 Nov 6"
COMMENT     Sequence Label: b2AR->M71-IRES-tauGFP-ACNF TV
FEATURES             Location/Qualifiers
     source          1..18857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..574,575..577,578..586)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(168..185)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     regulatory      1751..1760
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1757..1759,1760..1762,1763..2476)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1757..1759,1760..1762,1763..2473)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2063..2082)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2410..2431
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    2523..2556
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    2583..2631
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(2638..3441)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2749..2768)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3359..3378
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     polyA_signal    4179..4227
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     intron          4577..4642
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(5468..5488)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(5512..5523)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    6198..6231
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(10160..10176)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10161..10177)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(10201..10220)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10202..10220)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10227..10244)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10227..10243)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10236..10258)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10385..10840
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10472..10491)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10682..10703
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10866..10970
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10971..11039,11040..11831)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11189..11208)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12002..12590
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12491..12510
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12744..12761
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12878..12899
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12914..12919,12920..12937,12938..12944)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12952..12968
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12957..12979
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12976..12992
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12976..12992
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        13013..13031
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        13074..13092
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(15099..15116)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(18545..18556)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
       61 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
      121 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
      181 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
      241 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
      301 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
      361 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctaagcgt attcaacaag
      421 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
      481 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
      541 gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatgg ctgagccccg
      601 ccaggagttc gacgtgatgg aagatcatgc tcagggggac tacaccctgc aagaccagga
      661 gggtgacatg gaccccggcc tgaaagagtc tcccctgcag accccggccg atgatggatc
      721 tgaggaacca ggctctgaaa cctctgatgc taagagcact ccgacggcgg aagatgcgac
      781 agcaccctta gtggatgagg gagcccccgg tgagcaggcg gccgctcagg cccccgcgga
      841 gatcccagaa ggaaccgcag ctgaagaagc aggcattggc gacacgtcca acctggaaga
      901 ccaagctgcc ggacacgtga cccaagctcg catggtcagt aaaggcaaag atgggactgg
      961 acccgatgac aaaaaaacca agggggcgga tggtaagcct ggaacgaaga ttgccacacc
     1021 ccggggagca gcccctccag gccagaaagg ccaggccaac gccacccgga ttccagcaaa
     1081 aaccactccc accccgaaga cctcgccagg tgaatctggg aaatccgggg accgcagcgg
     1141 ctacagcagc cccggctccc caggcactcc gggcagccgc tcccgcacac cctccctgcc
     1201 gaccccgccc acccgggagc ccaagaaggt ggcggtggtc cgcactcccc ccaagtcgcc
     1261 gtctgcagcc aagagccgcc tgcaggccgc tcccgggccc atgccagacc tgaagaacgt
     1321 caagtccaaa atcggctcca cggaaaacct gaagcaccag ccaggaggtg gcaaggtgca
     1381 gataattaat aagaagctgg atcttagcaa cgtccagtcc aagtgtggct caaaggataa
     1441 tatcaaacac gtgccaggag gcggcagtgt gcaaatagtc tacaaaccag tggatctgag
     1501 caaggtgacc tccaagtgtg gctcattagg caacatccat cataagccag gaggtggcca
     1561 agtggaagtg aaatctgaga agctggactt caaggataga gtccagtcga agattgggtc
     1621 cctggacaac atcacacacg tccctggcgg agggaataaa aagatcgaaa cccacaagct
     1681 gaccttccgc gagaacgcca aagccaagac cgaccacggg gcggagatcg tgtccgggga
     1741 tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     1801 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     1861 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     1921 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     1981 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     2041 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     2101 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     2161 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     2221 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     2281 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     2341 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     2401 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     2461 cgagctgtac aagtaaagcg gccgcgactc tagagattcg agatctagat atcgatgaat
     2521 tcataacttc gtataatgta tgctatacga agttatggat ctgtcgatcg acggatcgat
     2581 ccgaacaaac gacccaacac ccgtgcgttt tattctgtct ttttattgcc gatcccctca
     2641 gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc
     2701 gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt
     2761 agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc
     2821 agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac
     2881 gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag
     2941 cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg
     3001 tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt
     3061 atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga
     3121 tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt
     3181 gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc
     3241 tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg
     3301 gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc
     3361 ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc
     3421 ttgttcaatg gccgatccca tattggctgc acggatcctg aacggcagag gttacggcag
     3481 tttgtctctc ccccttccgg gagccacctt cttctccaac cgtcccggtc gcgctctcgg
     3541 cgcttctgag gagagaactg gctgagtgac gccctttata gattcgccct tgtgtcccgc
     3601 cccttccttt cccgccctcc cttgcgctac ggggccgccc gcaccggcct acacggagcg
     3661 cgcgcggcgg agttgttgac gctagggctc cggctccctg gttgggtgtt ctttctgacg
     3721 cgacaggagg aggagaatgt tcctggtcct gtcgtcctcc tttcgggttt cccgtgcact
     3781 caaaccgagg acttacagaa cggaggataa agttaggcca tttttactca gcttcggagt
     3841 tcaggctcat tttcagctaa agtctctcat tagtatcccc ccacacacat cgggaaaatg
     3901 gtttgtccta cgcatcggta atgaaggcgg ggcccttcgg gtcctccgga gcgggttccg
     3961 ggggtggggg gaaggaggga gggacgggac gggcctcgtt catgaatatt cagttcaccg
     4021 ctgaatatgc ataaggcagg caagatggcg cgtccaatca attggaagta gccgttatta
     4081 gtggagaggc cccaggacgt tggggcaccg cctgtgctct agtagcttta cggagccctg
     4141 gcgctcgatg ttcagcccaa gctttcgcga gctcgaccga acaaacgacc caacacccgt
     4201 gcgttttatt ctgtcttttt attgccgctc agctttacag tgacaatgac ggctggcgac
     4261 tgaatattag tgcttacaga cagcactaca tattttccgt cgatgttgaa atcctttctc
     4321 atatgtcacc ataaatatca aataattata gcaatcattt acgcgttaat ggctaatcgc
     4381 catcttccag caggcgcacc attgcccctg tttcactatc caggttacgg atatagttca
     4441 tgacaatatt tacattggtc cagccaccag cttgcatgat ctccggtatt gaaactccag
     4501 cgcgggccat atctcgcgcg gctccgacac gggcactgtg tccagaccag gccaggtatc
     4561 tctgaccaga gtcatcctaa aatacacaaa caattagaat cagtagttta acacattata
     4621 cacttaaaaa ttttatattt accttagcgc cgtaaatcaa tcgatgagtt gcttcaaaaa
     4681 tcccttccag ggcgcgagtt gatagctggc tggtggcaga tggcgcggca acaccatttt
     4741 ttctgacccg gcaaaacagg tagttattcg gatcatcagc tacaccagag acggaaatcc
     4801 atcgctcgac cagtttagtt acccccaggc taagtgcctt ctctacacct gcggtgctaa
     4861 ccagcgtttt cgttctgcca atatggatta acattctccc accgtcagta cgtgagatat
     4921 ctttaaccct gatcctggca atttcggcta tacgtaacag ggtgttataa gcaatcccca
     4981 gaaatgccag attacgtata tcctggcagc gatcgctatt ttccatgagt gaacgaacct
     5041 ggtcgaaatc agtgcgttcg aacgctagag cctgttttgc acgttcaccg gcatcaacgt
     5101 tttcttttcg gatccgccgc ataaccagtg aaacagcatt gctgtcactt ggtcgtggca
     5161 gcccggaccg acgatgaagc atgtttagct ggcccaaatg ttgctggata gtttttactg
     5221 ccagaccgcg cgcctgaaga tatagaagat aatcgcgaac atcttcaggt tctgcgggaa
     5281 accatttccg gttattcaac ttgcaccatg ccgcccacga ccggcaaacg gacagaagca
     5341 ttttccaggt atgctcagaa aacgcctggc gatccctgaa catgtccatc aggttcttgc
     5401 gaacctcatc actcgttgca tcgaccggta atgcaggcaa attttggtgt acggtcagta
     5461 aattggacac cttcctcttc ttcttgggca tggccgcagg aaagcagagc cctgaagctc
     5521 ccatcaccgg ccaataagag ccaagcctgc agtgtgacct catagagcaa tgtgccagcc
     5581 agcctgaccc caagggccct caggcttggg cacactgtct ctaggaccct gagagaaaga
     5641 catacccatt tctgcttagg gccctgagga tgagcccagg ggtggcttgg cactgaagca
     5701 aaggacactg gggctcagct ggcagcaaag tgaccaggat gctgaggctt tgacccagaa
     5761 gccagaggcc agaggccagg acttctcttg gtcccagtcc accctcactc agagctttac
     5821 caatgccctc tggatagttg tcgggtaacg gtggacgcca ctgattctct ggccagccta
     5881 ggacttcgcc attccgctga ttctgctctt ccagccactg gctgaccggt tggaagtact
     5941 ccagcagtgc cttggcatcc agggcatctg agcctaccag gtccttcagt acctcctgcc
     6001 agggcctgga gcagccagcc tgcaacacct gcctgccaag cagagtgacc actgtgggca
     6061 caggggacac agggtggggc ccacaacagc accattgtcc acttgtccct cactagtaaa
     6121 agaactctag ggttgcgggg ggtgggggag gtctctgtga ggctggtaag ggatatttgc
     6181 ctggcccatg gagatccata acttcgtata atgtatgcta tacgaagtta taagctttcg
     6241 cgagctcgaa tcggatccga attcttaatt aaaaatgctg atctttcttc atgtaaaagt
     6301 aagtggttat gcacacctgt atgataacct ctgcaagctc acccaactct gaacaggaaa
     6361 caggaatcat tgctctccat tgttgggctc aaggccaatc tccttgtcct cattctcatc
     6421 ttctctacca ttggttctat ttataattac tcgattttaa gataattttt atattattgg
     6481 gtctctgtcc tctatatgag gccaatggta agcaatttta taatttttac tattaatatt
     6541 tcatctatta tataatgtat ttgatcatat ccaccccacc ctcttcttct ctttcattat
     6601 caccccctat atatctacct cccattttca tgtcctcttt tttttttaat aaccaactgc
     6661 atccaattaa tactgtttat attcacatta gtgtctggcc atgcatggga gcatgggtaa
     6721 cctactagca aatctcacac aaattcattt aattagtgga atctaaatta cttctagggt
     6781 actagttgca agtgattctg agaaatgcag tttccagctt gttgacacta aatagaatgt
     6841 gtattaaagg gttagtagga cagtctcact taaatagctt tttactcatt ttcttctgac
     6901 ctttccccaa caaaagaaga aattgctacc actgttgata tgctctaatt agaactaaag
     6961 gtaggaaact atctcagagg gagacctctc gatgcaacaa caacaacaac aacaacaaaa
     7021 aggcaaaagt gtaattctga gttagaatac atgcctgaag agggtgaggc actgaatttg
     7081 acgtgatgta atcagtttca caatttactt atattggttt taaatattgt aatctgcaaa
     7141 gtgggcaaaa ccagggaaag ataattctgt gttccagaca agacagaaca atcagatcca
     7201 aatctgtaga gcaggttatt tccacagaaa catagtgctt ctcagttcaa gctgctcgcc
     7261 caaataaagc tctggagagg cgacttgtga agcatggaac acctcactag actgcccatg
     7321 gattttagcc caagaaaagt ctttgtcacc agagctagga catttagttt tatttcctgg
     7381 ttagtcatga attttccctc ttaaacttta ccttttcata gagttgactc cactatactt
     7441 agaaccacaa actctcactg tcaacaacag catcctcatt gtgaatacct tccatggaaa
     7501 gaccagaagt cttttctctt tagccttgac tttacttcag tttattacaa gcacaaatgt
     7561 atttttttca ttatttggaa ttgatcaatt tgatggcatt gtgcaaattg ctttaccaat
     7621 tggatgaatt ttctcaaaag tgtagttttt aaagagaagt ttattttgaa gatctttatg
     7681 aaactgccta taatagagag gagctgtggc agactaaaca ccaaaaatct ttcctctgcc
     7741 tttgtgctaa aactgttgtt tctaacacac gcacacaaaa atctccaaca gtaggcgtac
     7801 ataatctggc atacatcact gctcttaaaa actttttaaa ctgccgggca gtggtggtgc
     7861 atgtctttaa tcccagcatt tgggaagcag aggcaggcaa atttctgagt tcaaagccag
     7921 cctggtctac agagagagtt ccagaacagc ccaggttata caacgaaacc ctgtctcaaa
     7981 taaataaata aataaataaa taaataaata accttttaaa ctaatgttag tatcttcatg
     8041 atctcaaact gatttaggaa aataacgtgt ccttctttag tgtttggtat agagagtata
     8101 ggctcttcac taaatattta caggaatcct ttgtgaataa cttcaaagta tacaaggcca
     8161 ttcctaaatt tataagcttg cactatagat ttttgttgaa ctgtagtcac acatcttgtg
     8221 gttttcaaaa atagtatatc taacttatga aatgtattat tagccctttt cttcttttcc
     8281 ccttcttttc tcatgactga aggtatgaag ccagttcaat gatgtgttca tctgtgtact
     8341 aaatactgat agtgtttgtg tgcacaaagg ctaaaaaggc aaaaattttt ggaagaagag
     8401 tttgctctac ctgaaacctg taaaatacag tctttaaatg actatgtaac agtgaaaggt
     8461 tcttttgtac taagcactta gaaggtgagt gacaacaatg taggagaaaa ctaatttaag
     8521 gtcatttcca gtccttcctc ttagccactc aagagtacaa ctgtccatca ctcctaaaat
     8581 gtccaccctc ttgaacatct gtgaaattcc ctttttccct taattacctt ctaaatagtc
     8641 tcaattcctc acatatacct tctattttat tttttgcttc tggtcttcac taggacactg
     8701 tgtctgacag tttggccttg cccaccacat tcacatagct gtcgagtaat ggctccacat
     8761 cacccaagcc ttccccagca tctccactgt caacaagctg atgcctttta tggacatgtt
     8821 aagggaaact ttcagcagtg atttttttgt aggttttcat ctaagggtta ggtcttatga
     8881 agtttcctca atctacattt atcagctgat gtcattacac aggtcttgtt gaggttgtca
     8941 caattttata ggtgcagctt cttcgttgcc atatccaaag atgctatctc atagcaggta
     9001 gccggttcct ctagctctta taatctttcc accactcttc catgatgatc cctgagactt
     9061 aaatatagtg tctacatgta gttgcgaata tctgccatag tgtcagatat gaatcccctc
     9121 ctagttagca agccttaaga ctttgaggtt gttgccattc cctagctact gtgagtaaag
     9181 tggaaatgaa cacagtcaag caagtatctg ttgaggagaa cacccagtcc tttggcaata
     9241 taccaaagaa gaatacagct agatcctgtg ataatccagt tataactttc tgtgaatcct
     9301 ccacagtgat taccatagag gcttccctat ttgcaaccct agcaacaatc aataagtctt
     9361 tttgcctttc tctgaatcct agacatttgt ttcagttgtt ttcttaatcc taaccatctg
     9421 actggagtaa ggcaaaatct aaaagcagtt ttaatttgca ttttcatagt tgcaaaggaa
     9481 gttaaacaca tttttacata tttgtcagcc atttttattt cttcctctga gatctttctc
     9541 ttcaaatcca gggcctatta ggggtgggga gtggtcagtt aatgacctta atgactgagc
     9601 aaatggagtc tttctcagaa agtcttttac tacatctcta tcttagaggg gactgcctgt
     9661 gtactcttca agtggtttta gcatttaacg ttgcccatgg aagtttttga tccatttgga
     9721 atttttgtac caaataatag atgtgggact atttttattc ttctacatgt ggacatccag
     9781 ttttaccagc acaatttgtt gaagagtttg tctttatacc agtgtatatg tttaacattt
     9841 ttgtcaaata tcagatggct aaactcatgt ttcagttttc tgttctgtta ctctgattta
     9901 aatgcctgtt ggtttggcca tttattttgc tttgttttgt tgccagtacc atgcaatttt
     9961 tattactata gctctataac actggttgaa actgtgttct atctctctag cattgttctt
    10021 tctgctcagg atttctttgg ttatccttag tctcttatgg ttccatatgg attttaggac
    10081 agttttatta tttctgtgaa gatgttgtgg agatttttag tgggaattaa tgtttaaacg
    10141 aattcgatat caagcttatc gataccgtcg acctcgaggg ggggcccggt acccaattcg
    10201 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa
    10261 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
    10321 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
    10381 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
    10441 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
    10501 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
    10561 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
    10621 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
    10681 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
    10741 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
    10801 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
    10861 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
    10921 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
    10981 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
    11041 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
    11101 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
    11161 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
    11221 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
    11281 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
    11341 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
    11401 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
    11461 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
    11521 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
    11581 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
    11641 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
    11701 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
    11761 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
    11821 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    11881 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    11941 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    12001 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    12061 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    12121 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
    12181 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    12241 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    12301 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    12361 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    12421 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    12481 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    12541 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    12601 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    12661 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    12721 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    12781 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
    12841 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
    12901 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
    12961 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc gaaattaacc
    13021 ctcactaaag ggaacaaaag ctggagctcc accgcggtgg cggccgcgag ctcaattaac
    13081 cctcactaaa gggagtcgac tcgatcaaca tgcggatact tcattcctcc ttagaatagg
    13141 gaacaaaata ccaatggaag gagttacaga gacaaagttt ggagctaaga caaaaggata
    13201 gatcatccag agactactcc acccaggggt ccatcccata atcagccacc aaatgcagac
    13261 actattgcac atgccaccaa gattttgctg aaaggaccct gatatagctg tctcttgtga
    13321 ggctacgcca gtgcctggca aatacagaag tggatgctca cagtcatcta ttggatggaa
    13381 cacagggccc ccaatgaagg agctagagaa agaacccaag gaactgaagg ggtctgcaac
    13441 cctataggtt gaacaacaat ataaactaac cagtaccccc agagctcgtg actctagctg
    13501 catatgtagc agaggatggc ctagtcagcc atcattggga agagaggccc cttgatcttg
    13561 caaactttat atgccccagt acagggcaac tccagggcca agaagtggga gtgggtgggt
    13621 aggggagcag ggcggggggg ggggtgtata gggaactttc aggatagcat ttgaaatgta
    13681 aataaagaaa atatctaata aaatttaaaa aaaaaaagaa aagaaaactg tcccagaaag
    13741 aacaataaaa atattctatt aaacttgcca gacttctctt ttaagaaggc tataaggggg
    13801 aaagtgtaga aaacagacac agagaaacaa gattaactga caatgtataa taacactcat
    13861 gtgtaatggc actgaaaatg atgttgcagg tagtcactgg gtacctatag gaatgaaaat
    13921 aatgataatg atgatacatt ggtagtaatt tgtagtactg atttcctgtc agaaaagatc
    13981 tggaaacaat gatcttccag aagcaatgag cttctctggc atcagattca cattcctggg
    14041 gtgtcatttc cctactgaaa gaaccaagat tcttggtgaa atgaatgact cagctctgaa
    14101 gaatgaaaag aatgtggtaa agtcacaaca tcttgtcata ccagatggta aggaagatat
    14161 tagagactag tatattaagt tcagagacaa attgagaagt ccagagactg tcaatacctg
    14221 gggcagtagc ttactggtta agtgtgcgca ctgctgttgc tgagcacctg agtgtggttc
    14281 tcagcacata gctcaactta taactcaagg tccaaggggg aatttattgc tgtcccctcc
    14341 aaaggtattt gtgttcatgt gaacatacca acacagacac atgtacaatt taaaataatg
    14401 aaaataaaat gtaaaacata aaattaaatt ctgataaaag catagtaatt gtattgggct
    14461 gaagcacatt aaatatgttc acactagtga atttataaag ggggggatat aagaaaccaa
    14521 ctattgtttt aaaattataa aacatatctt tttattataa ctgtaatagc aaatacagga
    14581 actattctgt tgaatagata gcacaaaaat aagcaccaaa atgagtgtga ttaaaattgg
    14641 agagctctga ataaaattaa aactagtgtc aatattctgg ccatgatgag tacagctgtt
    14701 ttcaaagaca ttactctctc cccgggaagg aattttagca acaggcttcc actttgtgct
    14761 tcaagttaaa attgcaaaac aaagaagcca ggacctgaat ttgagaaata atgtgtgtca
    14821 tttgtctttc tgtccctgag ttgcctcact tggcaaaact ttgtttcaac aaaactcttt
    14881 aaaacaagca tgtcaaggct agctcatgat gcatcatggt gactagctaa caatatgtaa
    14941 taaccatcat ggcactgaga atgatgttgc tggtagttac tgtggtgcct attgagatga
    15001 aaatgacaat aatgataata catcagtagt aaaggtgata gccatggtca taatggtaat
    15061 gatgataagg atgggtgggt agtggtgatg gatggttggt ggtggtggtg gtgatggtag
    15121 tggtaggggg gtaatggtgg tggtaacggc tctgttgatg ctaaattgtt cattgcccca
    15181 ttatattcta agtttctgaa actgaaagat gacttttaca gataaaagaa gaattaacac
    15241 acttgggaaa taaaacatga ttcacagaac aagagaaaac atgaactaat tgttacttta
    15301 gagacaacaa tggtctctag agtgactata tcccaggaga tgatccacac acacacacac
    15361 atatatataa acagaacccc caatttttag tcatattata tcacctttcc aaatgacaat
    15421 ctccttttcc ggactagact gggctcttta agtaaatttt gtggtgtata cacatctgca
    15481 tacttctaga atttactgaa tattgaagcc acagacattg cctgaagttt catttgagac
    15541 gctgtttcat ttccttcagc acaggtattt tgtctaaaaa ttagatgaga aaatatcttc
    15601 tcttgtgagc tcttctttat agtttagact atttatgacc atatgttgac agttaacttt
    15661 taatgctctg ttctatgtga tgtactctct tcagaaaaaa tcctgagctt ctgaaagctt
    15721 gataataata gctaatacac ataggaactc agaaggtatg tgtggtccta gttggtcttt
    15781 tatatcatta gaaaatttaa gctgtacctg atagctaaca ctgctacctg tattttcagc
    15841 acttggtcag ctaaggcagg agaatcatta caagctgaag gttaacctgg gctttggagt
    15901 gagatcttgt ctcaaaaaac agtagtggga gacctggaga ggtggctcag cagttaagag
    15961 cactgactgc tcatccagag gtcatgagtt caaatcccaa aaaccatctg taatgagatc
    16021 tgatgccctc ttctggtgta tctgaagaca gcaacagcaa cagtgtattc ataagcataa
    16081 aataaataaa tacatcttta aaaagaatca gtagtgggga aaaagacaag aataaaaagc
    16141 tgcagaagaa gattatataa gcaaaaaaaa aaaattgaga ctagctgtct gagctcattc
    16201 tacaagaatg tattctcatc tacattgcac tgggtatata acatttaccc tgacctgatt
    16261 tacaaagtat tttaatctct gctaatggtt actgaatatg tttcttataa tattaagaag
    16321 cagagattcc ataaagaggg ataggtaata tttttaatta ttcatcatct agataaaaat
    16381 cattttgtag ccgggcttgt ggcacacgcc tttaatccca gcacttggga ggcagaggca
    16441 ggcagatttc tgagttcaag gccagcctgg tctacaaggt gagttccagg acagccaggg
    16501 ctatacagag aaactctgtc tcaaaaatta aaaaaagatc attttgtagt aaataaaaat
    16561 aatattcaag atcataccga tctctgatat gtcaatatgt aactttagtt aagaaacgtt
    16621 ctatcactaa ttcaatatgt aaaccctaca tataacacat atgttctaat attcaaaata
    16681 ttccaaagat gttaaagatt ctcttatact taagaaaaag gaagtcactt gactcagtag
    16741 atgaagccat tttccatcaa atcttaaaac ttgagtttta tccctagaag ctttaagaaa
    16801 caaggagaaa gccaatttct gcaagttgta tatacacaca caacacagca cagcacactc
    16861 actatacaca aaatctcttt tcacaaaact ggtactataa gtgacttttg aaagaatgaa
    16921 ctgaagatgt gtcaataatt aataattgta tcaaaaacaa ttaccatagt gagattcctg
    16981 ctatgcctta agaaatgtat cccatactgt acaggggcag cacatgtctg caatcccagc
    17041 cagcaggaag aagaagaagg ggtaagttca aggaaaacca atgctacata gaaagactta
    17101 actttcaaaa aaagaaaaag aaaaatagaa agaaatagag aataatacct cagatatgag
    17161 aatttaattt aatatgtatc acatctacta ataacaacac atgcttctta ggaaaatata
    17221 ctatatatgt atatataata tgtatgattt gacatagtga taaatggtgc aatggtttag
    17281 gatctaaaaa taatagctaa aattaacaac aaagtgcttg ctgatcccac aataagcaac
    17341 tactcatcaa atatgacagg acagcagtaa gaaatacaga acttggccct ttactaagct
    17401 ttaaggcctc tgaaacccta agggctggtc cttctgtcag caagaagcaa cagtatagag
    17461 aagatatgtg tgccacagcc agaatagctc agcaagtgtc aaatgcagaa aaccagctct
    17521 ccaagcaggg caagaacagc cattcacaaa ctccttctct cccaccacac aggcttccca
    17581 cccagacaca atggggccac acgggaacga cagcgacttc ttgctggcac ccaacggaag
    17641 ccgagcgcca gaccacgacg tcactcagga acgggacgaa gcgtgggttg tgggcatggc
    17701 catcctcatg tcggttatcg tcctggccat cgtgtttggc aacgtgctgg tcatcacggc
    17761 cattgccaag ttcgagcgac tacaaaccgt caccaactac ttcataatct ccttggcgtg
    17821 tgctgatcta gtcatgggcc tagcggtggt gccgtttggg gccagtcaca tccttatgaa
    17881 aatgtggaat tttggcaact tctggtgcga gttctggact tccattgatg tgttgtgcgt
    17941 cacagccagc atcgagaccc tgtgcgtgat tgcagtggat cgctatgttg ctatcacatc
    18001 gcccttcaag taccagagcc tgctgaccaa gaataaggcc cgagtggtca tcctgatggt
    18061 atggattgta tctggcctta cctccttttt gcctatccag atgcactggt accgtgccac
    18121 ccacaagaaa gctatcgatt gttacaccga ggagacttgc tgtgacttct tcacgaacca
    18181 ggcctacgcc atcgcgtcct cgattgtgtc tttctacgtg cccctggtgg tgatggtctt
    18241 tgtctattcc cgggtcttcc aggtggccaa aaggcagctg cagaagatag acaaatctga
    18301 aggaagattc cacgcccaaa acctcagcca ggtggagcag gatgggcgga gcggccacgg
    18361 actccgaagg tcctccaagt tctgcttgaa agagcacaaa gccctcaaga ctttaggcat
    18421 catcatgggc acattcaccc tctgctggct gcccttcttc attgtcaata tcgtgcacgt
    18481 tatcagggac aacctcatcc ctaaggaagt ttacattctc cttaactggt tgggctacgt
    18541 caactctgcc ttcaatcctc ttatctactg tcggagtcca gatttcagga ttgcctttca
    18601 agagcttctg tgccttcgca ggtcttcttc gaaaacctat gggaacggct actctagcaa
    18661 tagcaacggc agaacggact acacagggga gccaaacact tgtcagctgg ggcaggagag
    18721 agaacaggaa ctgctgtgtg aggatccccc aggcatggaa ggctttgtga actgtcaagg
    18781 tactgtgcct agccttagcg ttgactccca aggaaggaac tgtagtacaa atgactcgcc
    18841 actgtaatgt ttaatta
//
