LOCUS       sequence-211327-        3819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Omura M, Mombaerts P
  TITLE     Trpc2-expressing sensory neurons in the mouse main olfactory 
            epithelium of type B express the soluble guanylate cyclase Gucy1b2.
  JOURNAL   Mol Cell Neurosci. 2015 Mar;65:114-24. doi: 
            10.1016/j.mcn.2015.02.012. Epub 2015 Feb 19.
  PUBMED    25701815
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2015-03"; volume: "65:114-24. doi"; pages: " 
            10.1016/j.mcn.2015.02.012. Epub 2015 Feb 19"
COMMENT     Sequence Label: pISH-Gucy1b2-probe2
FEATURES             Location/Qualifiers
     source          1..3819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..23
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7..23
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     30..49
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        30..48
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      79..88
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      83..92
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(990..1008)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(991..1008)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1026..1042)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1026..1042)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1039..1061)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1050..1066
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1074..1080,1081..1098,1099..1104))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1119..1140
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1257..1274)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1428..2016)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1508..1527)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2187..2978,2979..3047))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2810..2829
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3048..3152)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3230..3685)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3367..3388)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3579..3598
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(3811..3819,1..14)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gacgttgtaa aacgacggcc agtgaattgt aatacgactc actatagggc gaattgggcc
       61 cgacgtcgca tgctcccggc cgccatggcg gccgcgggaa ttcgattcag cgatgttgtg
      121 acattcacca acatctgtgc ggcctgtgaa cctatccaaa tagtgaacat gctcaactca
      181 atgtactcca agtttgacag gttaaccaac atccatgatg tctataaagt agaaacaata
      241 ggtgatgctt acatggtggt aggtggagta ccagtacccg ttgaaagcca tgctcaaaga
      301 gttgctaatt ttgctctggg gatgagaatt tctgcaaaag aagtgatgaa tcctgtcact
      361 ggggaaccca tacagatcag agtgggaatc catactggac cagtcttagc aggtgttgtg
      421 ggagacaaga tgccccggta ctgcctgttt ggtgacaccg taaacacggc ctctaggatg
      481 gaaagtcacg ggcttcccaa caaagtgcat ctgagcccca cagcccacag ggctctggaa
      541 aacaaagggt ttgaaattgt cacgagaggg gagatagagg tgaaggggaa aggaaaaatg
      601 accacatact tcctgatccg gaacctgaat gccaccgagg ttgagatcat ggggcgacct
      661 tcagctctcg ctgatgggaa ggaagcaagt actcctagaa accaagtcaa gaagccccgt
      721 gctgtcctga gcaacatgga ccaccaccag cagcaagtct acaacagtga tccagcagac
      781 gcttctgatg aagtcacact tgctgggagt ccagtggcag ggcgagacga cacagatgca
      841 gtcaatagcc agtcatcacc agatgagacc aagacaagtg cctttgccag tgaccctgtg
      901 ttgtctgcca atcactagtg aattcgcggc cgcctgcagg tcgaccatat gggagagctc
      961 ccaacgcgtt ggatgcatag cttgagtatt ctatagtgtc acctaaatag cttggcgtaa
     1021 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     1081 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     1141 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     1201 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     1261 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1321 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1381 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1441 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1501 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1561 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1621 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1681 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1741 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1801 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1861 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1921 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1981 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     2041 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     2101 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     2161 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2221 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     2281 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     2341 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2401 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     2461 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     2521 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     2581 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     2641 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     2701 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     2761 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     2821 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     2881 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     2941 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3001 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3061 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3121 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgat
     3181 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga aattgtaagc
     3241 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     3301 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     3361 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     3421 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     3481 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     3541 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     3601 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     3661 cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac tgttgggaag
     3721 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     3781 ggcgattaag ttgggtaacg ccagggtttt cccagtcac
//
