LOCUS       sequence-211345-        9946 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral gene expression vector for phospho-MARK3_T221A 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9946)
  AUTHORS   Brown FC, Still E, Koche RP, Yim CY, Takao S, Cifani P, Reed C, 
            Gunasekera S, Ficarro SB, Romanienko P, Mark W, McCarthy C, de 
            Stanchina E, Gonen M, Seshan V, Bhola P, O'Donnell C, Spitzer B, 
            Stutzke C, Lavallee VP, Hebert J, Krivtsov AV, Melnick A, Paietta 
            EM, Tallman MS, Letai A, Sauvageau G, Pouliot G, Levine R, Marto JA,
            Armstrong SA, Kentsis A
  TITLE     MEF2C Phosphorylation Is Required for Chemotherapy Resistance in 
            Acute Myeloid Leukemia.
  JOURNAL   Cancer Discov. 2018 Apr;8(4):478-497. doi: 
            10.1158/2159-8290.CD-17-1271. Epub 2018 Feb 5.
  PUBMED    29431698
REFERENCE   2  (bases 1 to 9946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-17-1271"; journalName: "Cancer Discov"; date: 
            "2018-04"; volume: "8"; issue: "4"; pages: "478-497"
COMMENT     Sequence Label: pLX304-MARK3_T211A
FEATURES             Location/Qualifiers
     source          1..9946
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    105..126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(141..146,147..164,165..171)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    179..195
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     184..206
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     203..219
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     203..219
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     238..258
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        240..258
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        286..512
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             513..693
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    740..865
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1358..1591
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1776..1820
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1995..2495
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2414..2432
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2507..2902
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    2962..3079
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3196..3499
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3500..3703
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3654..3674
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    3726..3750
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5975..5999)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6001..6042
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6084..6672
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6137..6157)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6555..6566)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6744..6977
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7055..7176
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7092..7111)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7146..7165
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7216..7351
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7371..7390)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7372..7390)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7400..7417)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7400..7416)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7409..7431)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7558..8013
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7645..7664)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7855..7876
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8039..8143
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8144..8212,8213..9004)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8362..8381)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9175..9763
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9664..9683
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9917..9934
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
       61 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
      121 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
      181 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
      241 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa
      301 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
      361 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
      421 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
      481 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
      541 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      601 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      661 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      721 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      781 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      841 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      901 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      961 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     1021 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1081 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1141 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1201 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     1261 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     1321 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     1381 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     1441 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     1501 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     1561 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     1621 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     1681 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     1741 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     1801 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     1861 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     1921 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     1981 ccccgagggg acccttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct
     2041 gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca
     2101 cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc
     2161 ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac
     2221 aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg
     2281 cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga
     2341 gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt
     2401 tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct
     2461 ccctcgttga ccgaatcacc gacctctctc cccagggggt accaccatgg ccaagccttt
     2521 gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat
     2581 ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg
     2641 tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc
     2701 tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg
     2761 catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa
     2821 agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc
     2881 ctctggttat gtgtgggagg gcctgcagct gcagtagtaa gaattctaga tcttgagaca
     2941 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     3001 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     3061 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac tttggcgccg
     3121 gctcgagggg gcccggggtt attaatagta atcaattacg gggtcattag ttcatagccc
     3181 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     3241 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     3301 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     3361 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     3421 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     3481 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     3541 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     3601 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     3661 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc taactgtcgg
     3721 gatcaacaag tttgtacaaa aaagcaggct ccaccatgga gtgcagtaaa atgtccacta
     3781 ggaccccatt gccaacggtg aatgaacgag acactgaaaa ccacacgtca catggagatg
     3841 ggcgtcaaga agttacctct cgtaccagcc gctcaggagc tcggtgtaga aactctatag
     3901 cctcctgtgc agatgaacaa cctcacatcg gaaactacag actgttgaaa acaatcggca
     3961 aggggaattt tgcaaaagta aaattggcaa gacatatcct tacaggcaga gaggttgcaa
     4021 taaaaataat tgacaaaact cagttgaatc caacaagtct acaaaagctc ttcagagaag
     4081 taagaataat gaagatttta aatcatccca atatagtgaa gttattcgaa gtcattgaaa
     4141 ctgaaaaaac actctaccta atcatggaat atgcaagtgg aggtgaagta tttgactatt
     4201 tggttgcaca tggcaggatg aaggaaaaag aagcaagatc taaatttaga cagattgtgt
     4261 ctgcagttca atactgccat cagaaacgga tcgtacatcg agacctcaag gctgaaaatc
     4321 tattgttaga tgccgatatg aacattaaaa tagcagattt cggttttagc aatgaattta
     4381 ctgttggcgg taaactcgac gcgttttgtg gcagtcctcc atacgcagca cctgagctct
     4441 tccagggcaa gaaatatgac gggccagaag tggatgtgtg gagtctgggg gtcattttat
     4501 acacactagt cagtggctca cttccctttg atgggcaaaa cctaaaggaa ctgagagaga
     4561 gagtattaag agggaaatac agaattccct tctacatgtc tacagactgt gaaaaccttc
     4621 tcaaacgttt cctggtgcta aatccaatta aacgcggcac tctagagcaa atcatgaagg
     4681 acaggtggat caatgcaggg catgaagaag atgaactcaa accatttgtt gaaccagagc
     4741 tagacatctc agaccaaaaa agaatagata ttatggtggg aatgggatat tcacaagaag
     4801 aaattcaaga atctcttagt aagatgaaat acgatgaaat cacagctaca tatttgttat
     4861 tggggagaaa atcttcagag ctggatgcta gtgattccag ttctagcagc aatctttcac
     4921 ttgctaaggt taggccgagc agtgatctca acaacagtac tggccagtct cctcaccaca
     4981 aagtgcagag aagtgtttct tcaagccaaa agcaaagacg ctacagtgac catgctggac
     5041 cagctattcc ttctgttgtg gcgtatccga aaaggagtca gaccagcact gcagatagtg
     5101 acctcaaaga agatggaatt tcctcccgga aatcaagtgg cagtgctgtt ggaggaaagg
     5161 gaattgctcc agccagtccc atgcttggga atgcaagtaa tcctaataag gcggatattc
     5221 ctgaacgcaa gaaaagctcc actgtcccta gtagtaacac agcatctggt ggaatgacac
     5281 gacgaaatac ttatgtttgc agtgagagaa ctacagctga tagacactca gtgattcaga
     5341 atggcaaaga aaacagcact attcctgatc agagaactcc agttgcttca acacacagta
     5401 tcagtagtgc agccacccca gatcgaatcc gcttcccaag aggcactgcc agtcgtagca
     5461 ctttccacgg ccagccccgg gaacggcgaa ccgcaacata taatggccct cctgcctctc
     5521 ccagcctgtc ccatgaagcc acaccattgt cccagactcg aagccgaggc tccactaatc
     5581 tctttagtaa attaacttca aaactcacaa ggagtcgcaa tgtatctgct gagcaaaaag
     5641 atgaaaacaa agaagcaaag cctcgatccc tacgcttcac ctggagcatg aaaaccacta
     5701 gttcaatgga tcccggggac atgatgcggg aaatccgcaa agtgttggac gccaataact
     5761 gcgactatga gcagagggag cgcttcttgc tcttctgcgt ccacggagat gggcacgcgg
     5821 agaacctcgt gcagtgggaa atggaagtgt gcaagctgcc aagactgtct ctgaacgggg
     5881 tccggtttaa gcggatatcg gggacatcca tagccttcaa aaatattgct tccaaaattg
     5941 ccaatgagct aaagctgtaa cccagtgatt atgaacccag ctttcttgta caaagtggtt
     6001 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct
     6061 aaccggtggc gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     6121 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     6181 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     6241 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     6301 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     6361 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     6421 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     6481 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     6541 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     6601 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     6661 ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca
     6721 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     6781 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6841 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     6901 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6961 gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata
     7021 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     7081 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7141 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     7201 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7261 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7321 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     7381 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     7441 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     7501 aagaggcccg caccgatcgc ccttcccaac agttgcgcag ctcaaatggc gaatgggacg
     7561 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     7621 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     7681 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     7741 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     7801 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     7861 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     7921 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     7981 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     8041 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     8101 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     8161 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     8221 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     8281 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     8341 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     8401 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     8461 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     8521 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     8581 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     8641 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     8701 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     8761 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     8821 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     8881 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     8941 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     9001 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     9061 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     9121 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     9181 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     9241 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     9301 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     9361 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     9421 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     9481 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     9541 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     9601 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     9661 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     9721 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     9781 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     9841 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     9901 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagag
//
