LOCUS       sequence-211347-        6792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dynamin-like protein 1 fused to mTurquoise2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Pablo Rivera-Fuentes
  TITLE     mTurquoise2-labeled markers of mitophagy
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pmTurquoise2-Drp1
FEATURES             Location/Qualifiers
     source          1..6792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        162..465
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        466..669
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     620..640
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      708..717
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(714..716,717..719,720..1430)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(759..780)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1020..1039)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1367..1388
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3681..3802
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3718..3737)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3772..3791
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3809..4264)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3946..3967)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4158..4177
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4291..4395
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4397..4754
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4397..4588
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4420..4440)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4605..4740
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4789..5583
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4789..5583
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4843..4862)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5453..5472
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5771..5790)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5815..5862
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6191..6779
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6680..6699
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
       61 tatcccctga ttctgtggat aaccgtatta ccgccatgca ttagttatta atagtaatca
      121 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      181 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      241 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      301 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      361 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      421 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      481 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      541 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      601 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      661 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc accatggtga
      721 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      781 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
      841 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
      901 ccaccctgtc ctggggcgtg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
      961 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     1021 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     1081 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     1141 agtacaacta ctttagcgac aacgtctata tcaccgccga caagcagaag aacggcatca
     1201 aggccaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact
     1261 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     1321 gcacccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     1381 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca
     1441 gatctcgagt gatggaggcg ctaattcctg tcataaacaa gctccaggac gtcttcaaca
     1501 cggtgggcgc cgacatcatc cagctgcctc aaatcgtcgt agtgggaacg cagagcagcg
     1561 gaaagagctc cgtgctagaa agcctggtgg ggagggacct gcttcccaga ggtactggaa
     1621 ttgtcacccg gagacctctc attctgcaac tggtccatgt ttcacaagaa gataaacgga
     1681 aaacaacagg agaagaaaat ggagtggaag cagaagaatg gggtaaattt cttcacacca
     1741 aaaataagct ttacacggat tttgatgaaa ttcgacaaga aattgaaaat gaaacagaaa
     1801 gaatttcagg aaataataag ggagtaagcc ctgaaccaat tcatcttaag attttttcac
     1861 ccaacgttgt caatttgaca cttgtggatt tgccaggaat gaccaaggtg cctgtaggtg
     1921 atcaacctaa ggatattgag cttcaaatca gagagctcat tcttcggttc atcagtaatc
     1981 ctaattccat tatcctcgct gtcactgctg ctaatacaga tatggcaaca tcagaggcac
     2041 ttaaaatttc aagagaggta gatccagatg gtcgcagaac cctagctgta atcactaaac
     2101 ttgatctcat ggatgcgggt actgatgcca tggatgtatt gatgggaagg gttattccag
     2161 tcaaacttgg aataattgga gtagttaaca ggagccagct agatattaac aacaagaaga
     2221 gtgtaactga ttcaatccgt gatgagtatg cttttcttca aaagaaatat ccatctctgg
     2281 ccaatagaaa tggaacaaag tatcttgcta ggactctaaa caggttactg atgcatcaca
     2341 tcagagattg tttaccagag ttgaaaacga gaataaatgt tctagctgct cagtatcagt
     2401 ctcttctaaa tagctacggt gaacccgtgg atgataaaag tgctacttta ctccaactta
     2461 ttaccaaatt tgccacagaa tattgtaaca ctattgaagg aactgcaaaa tatattgaaa
     2521 cttcggagct atgcggtggt gctagaattt gttatatttt ccatgagact tttgggcgaa
     2581 ccttagaatc tgttgatcca cttggtggcc ttaacactat tgacattttg actgccatta
     2641 gaaatgctac tggtcctcgt cctgctttat ttgtgcctga ggtttcattt gagttactgg
     2701 tgaagcggca aatcaaacgt ctagaagagc ccagcctccg ctgtgtggaa ctggttcatg
     2761 aggaaatgca aaggatcatt cagcactgta gcaattacag tacacaggaa ttgttacgat
     2821 ttcctaaact tcatgatgcc atagttgaag tggtgacttg tcttcttcgt aaaaggttgc
     2881 ctgttacaaa tgaaatggtc cataacttag tggcaattga actggcttat atcaacacaa
     2941 aacatccaga ctttgctgat gcttgtgggc taatgaacaa taatatagag gaacaaagga
     3001 gaaacaggct agccagagaa ttaccttcag ctgtatcacg agacaaggtt gcatctggag
     3061 gtggtggggt tggagatggt gttcaagaac caaccacagg caactggaga ggaatgctga
     3121 aaacttcaaa agctgaagag ttattagcag aagaaaaatc aaaacccatt ccaattatgc
     3181 cagccagtcc acaaaaaggt catgccgtga acctgctaga tgtgccagtt cctgttgcac
     3241 gaaaactatc tgctcgggaa cagcgagatt gtgaggttat tgaacgactc attaaatcat
     3301 attttctcat tgtcagaaag aatattcaag acagtgtgcc aaaggcagta atgcattttt
     3361 tggttaatca tgtgaaagac actcttcaga gtgagctagt aggccagctg tataaatcat
     3421 ccttattgga tgatcttctg acagaatctg aggacatggc acagcgcagg aaagaagcag
     3481 ctgatatgct aaaggcatta caaggagcca gtcaaattat tgctgaaatc cgggagactc
     3541 atctttggtg aggatccacc ggatctagat aactgatcat aatcagccat accacatttg
     3601 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     3661 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     3721 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     3781 ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc
     3841 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     3901 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     3961 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     4021 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     4081 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     4141 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     4201 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     4261 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4321 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4381 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
     4441 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4501 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4561 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     4621 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     4681 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     4741 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     4801 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     4861 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     4921 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
     4981 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     5041 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     5101 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     5161 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     5221 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     5281 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc
     5341 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     5401 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     5461 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     5521 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     5581 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     5641 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     5701 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg
     5761 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
     5821 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt
     5881 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
     5941 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
     6001 acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt
     6061 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     6121 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     6181 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     6241 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6301 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     6361 gccaccactt caagaactct gtatcaccgc ctacatacct cgctctgcta atcctgttac
     6421 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6481 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6541 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6601 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6661 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6721 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6781 acgccagcaa cg
//
