LOCUS       sequence-211356-         678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes
            Cas9 and DsRed2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Luo J, Lu L, Gu Y, Huang R, Gui L, Li S, Qi X, Zheng W, Chao T, 
            Zheng Q, Liang Y, Zhang L
  TITLE     Speed genome editing by transient CRISPR/Cas9 targeting and large 
            DNA fragment deletion.
  JOURNAL   J Biotechnol. 2018 Jun 7;281:11-20. doi: 
            10.1016/j.jbiotec.2018.06.308.
  PUBMED    29886029
REFERENCE   2  (bases 1 to 678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Biotechnol."; date: "2018-06-7"; volume: "281:11-20. doi"; pages: " 
            10.1016/j.jbiotec.2018.06.308"
COMMENT     Sequence Label: pX458-DsRed2
FEATURES             Location/Qualifiers
     source          1..678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..678
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(181..201)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     593..616
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 atggcctcct ccgagaacgt catcaccgag ttcatgcgct tcaaggtgcg catggagggc
       61 accgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      121 cacaacaccg tgaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
      181 ctgtcccccc agttccagta cggctccaag gtgtacgtga agcaccccgc cgacatcccc
      241 gactacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      301 gacggcggcg tggcgaccgt gacccaggac tcctccctgc aggacggctg cttcatctac
      361 aaggtgaagt tcatcggcgt gaacttcccc tccgacggcc ccgtgatgca gaagaaaacc
      421 atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag
      481 acccacaagg ccctgaagct gaaggacggc ggccactacc tggtggagtt caagtccatc
      541 tacatggcca agaagcccgt gcagctgccc ggctactact acgtggacgc caagctggac
      601 atcacctccc acaacgagga ctacaccatc gtggagcagt acgagcgcac cgagggccgc
      661 caccacctgt tcctgtag
//
