LOCUS       sequence-211359-       11454 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of flag tagged GREB1a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11454)
  AUTHORS   Haines CN, Braunreiter KM, Mo XM, Burd CJ
  TITLE     GREB1 isoforms regulate proliferation independent of ERalpha 
            co-regulator activities in breast cancer.
  JOURNAL   Endocr Relat Cancer. 2018 Jul;25(7):735-746. doi: 
            10.1530/ERC-17-0496. Epub 2018 Apr 25.
  PUBMED    29695586
REFERENCE   2  (bases 1 to 11454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1530/ERC-17-0496"; journalName: "Endocr Relat Cancer"; date: 
            "2018-07"; volume: "25"; issue: "7"; pages: "735-746"
COMMENT     Sequence Label: pcDNA-3xflagGREB1a
FEATURES             Location/Qualifiers
     source          1..11454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      896..905
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             905..970
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(6879..6896)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6885..7109
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7155..7583
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7242..7261)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7452..7473
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7592..7612)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7597..7926
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7777..7912
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7839..7858
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7975..9000
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7975..9000
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    9130..9251
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9167..9186)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9221..9240
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9300..9316)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9300..9316)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9313..9335)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9324..9340
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9348..9354,9355..9372,9373..9378))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9393..9414
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9531..9548)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9702..10287)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9782..9801)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10458..11249,11250..11318))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11081..11100
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11319..11423)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctgggccac
      901 catggactac aaagaccatg acggtgatta taaagatcat gatatcgatt acaaggatga
      961 cgatgacaag ctagcgttta aacttaagct tggtaccatg ggaaattctt acgctggaca
     1021 gctgaagacg acacgctttg aagaggtctt gcacaattcc atcgaggcat ccctgcggtc
     1081 caacaacctg gtgcccaggc ccatcttttc ccagctgtac ctggaagctg agcagcagct
     1141 tgccgctcta gaaggtggta gccgagtgga caatgaggaa gaggaagaag agggagaagg
     1201 agggctggaa acaaatggcc ccccaaaccc tttccagctg caccctctgc ctgaaggatg
     1261 ctgtaccaca gacgggtttt gccaggccgg gaaggacctg cgccttgtct ccatttccaa
     1321 cgagcccatg gatgtccctg cgggctttct cctcgtgggg gtcaagtccc ccagcctgcc
     1381 ggaccatctc ctggtgtgcg ccgttgacaa gaggttcttg ccagatgaca atggccacaa
     1441 tgctcttctt ggtttctctg ggaattgtgt tggctgtgga aagaaaggct tctgttactt
     1501 cacggaattc tccaatcata taaatctgaa actgaccact caacccaaga agcagaaaca
     1561 cttgaagtat tacctggtcc gtaatgcaca agggactcta accaaaggac ctttaatctg
     1621 ttggaaaggc tcagagttta gaagccggca gatccccgcc agtacttgtt ccagttccct
     1681 cttcccagcc ctggagagca cggctgcctt ccccagcgag cccgttcctg ggacgaaccc
     1741 cagcatcctg atgggagctc agcaggcagg accagcttct gatcacccct cactaaacgc
     1801 agcaatgggt ccggctgttt tcaacggcaa agattccccg aagtgccaac aactggcaaa
     1861 gaataacctg ttggccctgc cgcgaccatc ggctttaggt atcttgtcaa actccgggcc
     1921 ccccaaaaaa cgccacaaag ggtggtctcc agaatctcca tcagctccag atggtggctg
     1981 cccccaaggt ggtgggaaca gagctaagta tgagagcgca ggcatgtcct gcgtgccgca
     2041 ggttggcttg gtgggaccag cttcagtcac ctttccagtg gtggcctctg gagaaccagt
     2101 gtctgttcct gacaacttgc tgaaaatatg caaggccaag ccagtgatat ttaaaggcca
     2161 tgggaacttc ccttacctct gtgggaacct gaatgacgtc gtggtcagcc ccctcttgta
     2221 cacgtgctac cagaattccc agtctgtctc acgggcatac gagcagtacg gcgcctctgc
     2281 catccagccc atctccgagg agatgcagct cctgcttacc gtctactacc tggtccagct
     2341 ggccgcggac caggtgccct tgatggagga cctggagcag atcttcctgc gctcttggcg
     2401 cgagtcgcac ctgaccgaga tccggcagta ccagcaggcg ccgccgcagc ccttcccgcc
     2461 cgcgcccagc gccgcggcac ccgtgacctc cgcgcagctg ccctggctgg ccagcctggc
     2521 cgccagctcc tgcaacgaca gcgtgcacgt catcgagtgt gcttactccc tggccgaggg
     2581 cctctccgag atgttccggc tgttggtcga gggcaagctt gccaagacca actacgtggt
     2641 catcatctgc gcctgccgca gcgcggccat cgactcctgc atcgccgtca ccggaaaata
     2701 ccaagcccgg attctttccg agagccttct cactcctgcg gagtaccaga aggaagtcaa
     2761 ttacgagctg gttacgggga aggtagactc gctgggggcc ttctttagca ccctctgtcc
     2821 agagggtgac attgacattt tgctggacaa atttcaccag gaaaatcaag gccatatttc
     2881 ttcctcactc gctgcctctt ctgtcactaa agcagcatcc ctggatgtca gtgggacacc
     2941 ggtgtgcaca agttacaatc tggagccaca cagcatccgg cccttccagc tggcagtagc
     3001 gcagaagctc ctctcccatg tgtgttccat tgcggattcc agcacccaaa atctggacct
     3061 gggatccttt gagaaggtgg actttctcat ttgcattccc ccctcagaag tgacctacca
     3121 gcagactctg ctccacgtgt ggcattcagg ggttttgctg gagcttggtc tgaagaaaga
     3181 gcacatgacg aagcagaggg tggaacagta tgttctgaag ctagacacgg aggcacagac
     3241 aaaatttaag gcttttctgc aaaactcctt ccagaacccg catacacttt ttgtcctaat
     3301 ccatgaccat gcgcactggg atcttgtgag tagcactgtt cataacctct attctcaaag
     3361 tgacccgtcg gtgggattgg tggaccgatt gctcaactgc agggaggtga aggaggcccc
     3421 caacattgtg acacttcacg tgacctcctt cccgtatgca ctgcagacac agcacaccct
     3481 catcagcccc tacaacgaga tccactggcc tgcctcctgc agtaatggag tggacttata
     3541 tcatgaaaat aagaagtact tcgggctgtc ggagtttatt gaatccaccc tttcaggaca
     3601 cagcctcccc ttgctcagat acgatagctc ctttgaggcc atggtcactg cattaggaaa
     3661 aaggttcccc cgcctgcaca gcgcggtgat caggaccttt gttctcgtgc agcactacgc
     3721 ggccgccctg atggccgtaa gcggcctccc gcagatgaag aactacacgt cggtggagac
     3781 gctggagatc acgcagaacc tcctcaactc cccgaagcag tgcccctgcg gccacgggct
     3841 catggtcctg ctgcgggtgc cctgttcgcc cctggcggtg gtggcctatg agcggctggc
     3901 ccacgtgcgg gcccggctgg cgctggagga gcactttgag atcatcctgg gcagtcccag
     3961 ctcaggcgtc accgtgggga agcacttcgt aaagcagctc aggatgtggc agaaaatcga
     4021 ggatgtggag tggagacccc agacttactt ggagctggag ggtctgcctt gcatcctgat
     4081 cttcagtggg atggacccgc atggggagtc cttgccgagg tctttgaggt actgtgacct
     4141 gcgattgata aactcctcct gcttggtgag aacagccttg gagcaggagc tgggcctggc
     4201 tgcctacttt gtgagcaacg aggttccctt ggagaagggg gctaggaacg aggccttgga
     4261 gagtgatgct gagaagctga gcagcacaga caacgaggat gaggagctgg ggacagaagg
     4321 ctctacctcg gagaagagaa gccccatgaa aagggagagg tcccgctccc acgactcagc
     4381 atcctcatcc ctctcctcca aggcttccgg ttcagcgctc ggtggcgagt cctcggctca
     4441 gcccacagca ctcccccagg gagagcatgc caggtcgccc cagccccgtg gccccgcaga
     4501 ggagggcaga gcccctggtg agaaacagag gccccgggca agtcaggggc caccctcggc
     4561 catcagcagg cacagtcccg ggccgacgcc ccagcccgac tgtagcctca ggaccggcca
     4621 gaggagcgtc caggtgtcgg tcacctcgtc gtgctcccag ctgtcctcct cctcgggctc
     4681 atcctcctca tccgtggcgc ccgctgccgg cacgtgggtc ctgcaggcct cccagtgctc
     4741 cttgaccaag gcctgccgcc agccacccat tgtcttcttg cccaagctcg tgtacgacat
     4801 ggttgtgtcc actgacagca gtggcctgcc caaggccgcc tccctcctgc cctccccctc
     4861 ggtcatgtgg gccagctctt tccgccccct gctcagcaag accatgacat ccaccgagca
     4921 gtccctctac taccggcagt ggacggtgcc ccggcccagc cacatggact acggcaaccg
     4981 ggccgagggc cgcgtggacg gcttccaccc ccgcaggctg ctgctcagcg gcccccctca
     5041 gatcgggaag acaggtgcct acctgcagtt cctcagtgtc ctgtccagga tgcttgttcg
     5101 gctcacagaa gtggatgtct atgacgagga ggagatcaat atcaacctca gagaagaatc
     5161 tgactggcat tatctccagc ttagcgaccc ctggccagac ctggagctgt tcaagaagtt
     5221 gccctttgac tacatcattc acgacccgaa gtatgaagat gccagcctga tttgttcgca
     5281 ctatcagggt ataaagagtg aagacagagg gatgtcccgg aagccggagg acctttatgt
     5341 gcggcgtcag acggcacgga tgagactgtc caagtacgca gcgtacaaca cttaccacca
     5401 ctgtgagcag tgccaccagt acatgggctt ccacccccgc taccagctgt atgagtccac
     5461 cctgcacgcc tttgccttct cttactccat gctaggagag gagatccagc tgcacttcat
     5521 catccccaag tccaaggagc accactttgt cttcagccaa cctggaggcc agctggagag
     5581 catgcgacta cccctcgtga cagacaagag ccatgaatat ataaaaagtc cgacattcac
     5641 tccaaccacc ggccgtcacg aacatgggct ctttaatctg taccacgcaa tggacggtgc
     5701 cagccatttg cacgtgctgg ttgtcaagga atacgagatg gcaatttata agaaatattg
     5761 gcccaaccac atcatgctgg tgctccccag tatcttcaac agtgctggag ttggtgctgc
     5821 tcatttcctc atcaaggagc tgtcctacca taacctggag ctcgagcgga accggcagga
     5881 ggagctggga atcaagccgc aggacatctg gcctttcatt gtgatctctg atgactcctg
     5941 cgtgatgtgg aacgtggtgg atgtcaactc tgctggggag agaagcaggg agttctcctg
     6001 gtcggaaagg aacgtgtctt tgaagcacat catgcagcac atcgaggcgg cccccgacat
     6061 catgcactac gccctgctgg gcctgcggaa gtggtccagc aagacccggg ccagcgaggt
     6121 gcaagagccc ttctcccgct gccacgtgca caacttcatc atcctgaacg tggacctgac
     6181 ccagaacgtg cagtacaacc agaaccggtt cctgtgtgac gatgtagact tcaacctgcg
     6241 ggtgcacagc gccggcctcc tgctctgccg gttcaaccgc ttcagcgtga tgaagaagca
     6301 gatcgtggtg ggcggccaca ggtccttcca catcacatcc aaggtgtctg ataactctgc
     6361 cgcggtcgtg ccggcccagt acatctgtgc cccggacagc aagcacacgt tcctcgcagc
     6421 gcccgcccag ctcctgctgg agaagttcct gcagcaccac agccacctct tcttcccgct
     6481 gtccctgaag aaccatgacc acccagtgct gtctgtcgac tgttacctga acctgggatc
     6541 tcagatttct gtttgctatg tgagctccag gccccactct ttaaacatca gctgctcgga
     6601 cttgctgttc agtgggctgc tgctgtacct ctgtgactct tttgtgggag ctagcttttt
     6661 gaaaaagttt cattttctga aaggtgcgac gttgtgtgtc atctgtcagg accggagctc
     6721 actgcgccag acggtcgtcc gcctggagct cgaggacgag tggcagttcc ggctgcgcga
     6781 tgagttccag accgccaatg ccagggaaga ccggccgctc ttttttctga cgggacgaca
     6841 catctgaact agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     6901 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     6961 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     7021 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     7081 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     7141 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     7201 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     7261 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     7321 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     7381 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     7441 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     7501 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     7561 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     7621 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     7681 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     7741 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7801 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7861 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     7921 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgatgaaa
     7981 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc
     8041 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga
     8101 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat
     8161 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa
     8221 ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac
     8281 ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc
     8341 gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt
     8401 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg
     8461 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg
     8521 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac
     8581 aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc
     8641 ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg
     8701 gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc
     8761 cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat
     8821 ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg
     8881 actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta
     8941 gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag
     9001 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     9061 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     9121 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     9181 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     9241 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     9301 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     9361 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     9421 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     9481 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     9541 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     9601 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     9661 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     9721 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     9781 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     9841 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     9901 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     9961 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    10021 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    10081 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    10141 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    10201 agctcttgat ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag
    10261 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    10321 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    10381 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    10441 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    10501 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    10561 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    10621 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10681 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10741 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10801 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10861 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10921 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10981 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    11041 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    11101 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    11161 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    11221 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    11281 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    11341 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    11401 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
