LOCUS       sequence-211366-        8198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8198)
  AUTHORS   Munger K, Phelps WC, Bubb V, Howley PM, Schlegel R
  TITLE     The E6 and E7 genes of the human papillomavirus type 16 together are
            necessary and sufficient for transformation of primary human 
            keratinocytes.
  JOURNAL   J Virol. 1989 Oct . 63(10):4417-21.
  PUBMED    2476573
REFERENCE   2  (bases 1 to 8198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            1989 Oct . 63(10):4417-21."
COMMENT     Sequence Label: pB-actin 16 E6*
FEATURES             Location/Qualifiers
     source          1..8198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3164..3181)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3335..3923)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3415..3434)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4094..4885,4886..4954))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4717..4736
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4955..5059)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5127..5145
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ccacctgggc gctcagtgcc caagagatgt ccacacctag gatgtcccgc ggtgggtggg
       61 gggcccgaga gacgggcagg ccgggggcag gcctggccat gcggggccga accgggcact
      121 gcccagcgtg gggcgcgggg gccacggcgc gcgcccccag cccccgggcc cagcacccca
      181 aggcggccaa cgccaaaact ctccctcctc ctcttcctca atctcgctct cgctcttttt
      241 ttttttcgca aaaggagggg agagggggta aaaaaatgct gcactgtgcg gcgaagccgg
      301 tgagtgagcg gcgcggggcc aatcagcgtg cgccgttccg aaagttgcct tttatggctc
      361 gagcggccgc ggcggcgccc tataaaaccc agcggcgcga cgcgccacca ccgccgagac
      421 cgcgtccgcc ccgcgagcac agagcctcgc ctttgccgat ccgccgcccg tccacacccg
      481 ccgccaggta agcccggcca gccgaccggg gcatgcggcc gcggcccctt cgcccgtgca
      541 gagccgccgt ctgggccgca gcggggggcg catggggggg gaaccggacc gccgtggggg
      601 gcgcgggaga agcccctggg cctccggaga tgggggacac cccacgccag ttcggaggcg
      661 cgaggccgcg ctcgggaggc gcgctccggg ggtgccgctc tcggggcggg ggcaaccggc
      721 ggggtctttg tctgagccgg gctcttgcca atggggatcg cagggtgggc gcggcgtagc
      781 ccccgccagg cccggtgggg gctggggcgc cattgcgcgt gcgcgctggt cctttgggcg
      841 ctaactgcgt gcgcgctggg aattggcgct aattgcgcgt gcgcgctggg actcaaggcg
      901 ctaattgcgc gtgcgttctg gggcccgggg tgccgcggcc tgggctgggg cgaaggcggg
      961 ctcggccgga aggggtgggg tcgccgcggc tcccgggcgc ttgcgcgcac ttcctgcccg
     1021 agccgctggc cgcccgaggg tgtggccgct gcgtgcgcgc gcgccgaccc ggcgctgttt
     1081 gaaccgggcg gaggcggggc tggcgcccgg ttgggagggg gttggggcct ggcttcctgc
     1141 cgcgcgccgc ggggacgcct ccgaccagtg tttgcctttt atggtaataa cgcggccggc
     1201 ccggcttcct ttgtccccaa tctgggcgcg cgccggcgcc ccctggcggc ctaaggactc
     1261 ggcgcgccgg aagtggccag ggcgggggcg acttcggctc acagcgcgcc cggctattct
     1321 cgcaggatca gtcgactcta gaggatccac catgtttcag gacccacagg agcgacccag
     1381 aaagttacca cagttatgca cagagctgca aacaactata catgatataa tattagaatg
     1441 tgtgtactgc aagcaacagt tactgcgacg tgaggtgtat taaggtacca gatctgaatt
     1501 caagcttgga attctcatgt ttgacagctt atcatcgata agctactcag gatttaaaaa
     1561 ctggaacggt gaaggtgaca gcagtcggtt ggagcgagca tcccccaaag ttcacaatgt
     1621 ggccgaggac tttgattgca cattgttgtt tttttaatag tcattccaaa tatgagatgc
     1681 attgttacag gaagtccctt gccatcctaa aagccacccc acttctctct aaggagaatg
     1741 gcccagtcct ctcccaagtc cacacagggg aggtgatagc attgctttcg tgtaaattat
     1801 gtaatgcaaa atttttttaa tcttcgcctt aatacttttt tattttgttt tattttgaat
     1861 gatgagcctt cgtgcccccc cttccccctt ttttgtcccc caacttgaga tgtatgaagg
     1921 cttttggtct ccctgggagt gggtggaggc agccagggct tacctgtaca ctgacttgag
     1981 accagttgaa taaaagtgca caccttaaaa atgaggccaa gtgtgacttt gtggtgtggc
     2041 tgggttgggg gcagcagagg gtgaaccctg caggagggtg aaccctgcaa aagggtgggg
     2101 cagtgggggc caacttgtcc tcacccagag tgcaggtgtg tggagatccc tcctgccttg
     2161 acattgagca gccttagagg gtgggggagg ctcaggggtc aggtctctgt tcctgcttat
     2221 tggggagttc ctggcctggc ccttctatgt ctccccaggt accccagttt ttctgggttc
     2281 acccagagtg cagatgcttg aggaggtggg aagggactat ttgggggtgt ctggctcagg
     2341 tgccatgcct cactggggct ggttggcacc tgcatttcct gggagtgggg ctgtctcagg
     2401 gtagctgggc acggtgttcc cttgagtggg ggtgtagtga gtgttcctag ctgccacgcc
     2461 tttgccttca cctatgggat cgtggctgtc agccttgagg gtcagcctgg cccaggctcc
     2521 cataggctta ggagaggccg caattcctac ctgttcatcc agacagaggg ggacctggaa
     2581 tcaaagtcaa gttggggtgg ggggtccatg gggccatatc tggcctgcag acagctctgg
     2641 ttagctatgg gctgaggtct ggattctgcc ttgtgactgg agactgggcg ccatcccgtg
     2701 gcctctgagg gcccagtttg ttcgtgaccc atgctgcaga gggagatacc caggctcctg
     2761 gacctcaggg cctgcctggc actgcaggcc aggttctctt tttatttctg agacggggtc
     2821 ttgtgttgcc caggctggag tgtaatggca caaccatggc tcactgcagc ctcaactttg
     2881 ggctcaagca attctcctgc ctcagcctcc caagtagctg aaactacaca ggcatgcccc
     2941 agcacgccca gctagttgca ggtcagattc tcattgttct acagggccct gagttaaata
     3001 ctctcgcaag aagaaaccaa gcctggcctg ccttccctct gctagaagct gtgattgaca
     3061 ccagcagaga atgaaggaag aaaagggggt gaggatctat gcggtgtgaa ataccgcaca
     3121 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     3181 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     3241 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     3301 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     3361 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3421 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3481 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3541 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3601 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3661 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3721 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     3781 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3841 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3901 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3961 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     4021 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     4081 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     4141 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     4201 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     4261 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     4321 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     4381 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
     4441 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     4501 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     4561 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4621 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4681 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     4741 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4801 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     4861 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4921 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     4981 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     5041 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     5101 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cttatcatcg
     5161 aattccgact ttcaaacagc ctttggactc aggccttggg gacattccag gggacccttt
     5221 accagggagg ggcagtgtga ggcaggaggc agggctgccg cctcagggac cctgagccta
     5281 gagtccttcc caccgcattt ggggacttga gcacttcacc agtcctgact cagtttccta
     5341 gtctgtgaat gggggtgtgt ctcctggaat gttgcccccc ctcattcatt cattctcctc
     5401 tcactgagca cctaccgtgt tcagggtccc tgtcctccta gagctaactc aagtcttgga
     5461 gggaagctct gtctcagttc cttcctctac aaaatggagc ttgaggacgg gcacggtggc
     5521 tcatgcctgt aatcccagga ctttgggagg ctgaggtggg aggatcgctt gagccccccg
     5581 gagctggaga ccagcctggg caacatggtg agaagccata cctctacaga taatttaaaa
     5641 attagccagg cttggaagtg tgtgcctata gtcccagcta ctcgtgaagg ctttgagccc
     5701 aggaggtcga ggctgcagtg agctgtgatc gcactccagc ctgggcatca cagcaagacc
     5761 ttgtatcaaa aatagtaata ataataaaaa ggaggttgga ttccctcctg gcaggatagg
     5821 gagggcgctg cagtgcccag ggcaaggtgg ctgggtggtt gttttgcggg agggccaagg
     5881 agtggtccct gggtctgcgc tgtaagagtt ggttgcctgg aggccttgca gggtgggggt
     5941 gtcaccaaag caaaggcttg gaggggaaaa aacaaaagtc ccagccaggc aggtggagtc
     6001 cccttggctg agttcaaccg agggttctcc gggggctgcg tgcgtgcccc agtgacagct
     6061 ccgaaagctc ccttacaggg caaagttccc aagcacagaa gagaacctgt tcacttctcc
     6121 cctgctcggc ccgccccctg gccaggcacc tctacttcct cttttcctgc tccgctgctt
     6181 gctttctctc ttcagctcct ccctgcccct caccccaggc tgctcggcca cctccaacct
     6241 gccacctgag gacacccagg cagtcactca ttcaacagcg aggagccctg gggtgggtgt
     6301 agtgggaagg agtgggggtg acggagaccc tgggagggct cgcagcctgg tggctgaggc
     6361 ccagttctaa atgccagctg caagccttgg tctgaggtag ggaggaaggc gtggctgcag
     6421 aggctaaaac gcttccccaa agaggggctt tctgggatgg gacttgaagg gtgcatagga
     6481 gagcactagg aagtggccgc tgcagacaga gggaaccaca agccaggagg acaggccagg
     6541 aatgctgcag cccggggcgg ggtggggctg gagctcctgt ctcttggcca gctgaatgga
     6601 ggcccagcgg caacacaggt cctgcctggg gatcaggtct gctctgcacc ccaccttgct
     6661 gcctggagcc gcccacctga caacctctca tccctgctct gtagatccgg tcccatcccc
     6721 actgcccacc ccaccccccc agcactccac ccagttcaac gttccacgaa cccccagaac
     6781 cagccctcat caacaggcag caagaagggc cccccgccca tcgccccaca acgccagccg
     6841 ggtgaactgt agcgttggca ggtcctgagg cagctggcaa gacgcctgca gctgaaagat
     6901 acaaggccag ggacaggaca gtcccatccc caggaggcag ggagtataca ggctggggaa
     6961 gtttgccctt gcgtggggtg gtgatggagg aggctcagca agtcttctgg actgtgaacc
     7021 tgtgtctgcc actgtgtgct gggtggtggt catctttccc accaggctgt ggcctctgca
     7081 accttcaagg gaggagcagg tcccattggc tgagcacagc cttgtaccgt gaactggaac
     7141 aagcagcctc cttcctggcc acaggttcca tgtccttata tggactcatc tttgcctatt
     7201 gcgacacaca ctcaatgaac acctactacg cgctgcaaag agccccgcag gcctgaggtg
     7261 cccccacctc accactcttc ctatttttgt gtaaaaatcc agcttcttgt caccacctcc
     7321 aaggaggggg aggaggagga aggcaggttc ctctaggctg agccgaatgc ccctctgtgg
     7381 tcccacgcca ctgatcgctg catgcccacc acctgggtac acacagtctg tgattcccgg
     7441 agcagaacgg accctgccca cccggtcttg tgtgctactc agtggacaga cccaaggcaa
     7501 gaaagggtga caaggacagg gtcttcccag gctggctttg agttcctagc accgccccgc
     7561 ccccaatcct ctgtggcaca tggagtcttg gtccccagag tcccccagcg gcctccagat
     7621 ggtctgggag ggcagttcag ctgtggctgc gcatagcaga catacaacgg acggtgggcc
     7681 cagacccagg ctgtgtagac ccagcccccc cgccccgcag tgcctaggtc acccactaac
     7741 gccccaggcc tggtcttggc tgggcgtgac tgttaccctc aaaagcaggc agctccaggg
     7801 taaaaggtgc cctgccctgt agagcccacc ttccttccca gggctgcggc tgggtaggtt
     7861 tgtagccttc atcacgggcc acctccagcc actggaccgc tggcccctgc cctgtcctgg
     7921 ggagtgtggt cctgcgactt ctaagtggcc gcaagccacc tgactccccc aacaccacac
     7981 tctacctctc aagcccaggt ctctccctag tgacccaccc agcacattta gctagctgag
     8041 ccccacagcc agaggtcctc aggccctgct ttcagggcag ttgctctgaa gtcggcaagg
     8101 gggagtgact gcctggccac tccatgccct ccaagagctc cttctgcagg agcgtacaga
     8161 acccagggcc ctggcacccg tgcagaccct ggcccacc
//
