LOCUS       sequence-211370-         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RAMA domain from murine MPND.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Kweon SM, Chen Y, Moon E, Kvederaviciute K, Klimasauskas S, Feldman 
            DE
  TITLE     An Adversarial DNA N(6)-Methyladenine-Sensor Network Preserves 
            Polycomb Silencing.
  JOURNAL   Mol Cell. 2019 Apr 5. pii: S1097-2765(19)30222-9. doi: 
            10.1016/j.molcel.2019.03.018.
  PUBMED    30982744
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Apr 5. pii: S1097-2765(19)30222-9. doi: 
            10.1016/j.molcel.2019.03.018."
COMMENT     Sequence Label: pET30a-RAMA
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..61
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             68..112
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             599..622
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(702..720)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      716..763
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aggagatata catatgcacc atcatcatca tcantnttct ggtctggtgc cacgcggttc
       61 tggtatgaaa gaaaccgctg ctgctaaatt cgaacgccag cacatggaca gcccagatct
      121 ggcagctccc gagtctctgt ctcccggggc cacggccgag gaggcgccgg aggaggatga
      181 ggacgacgcg gaggccgagg accccgagcg cgggacgggt agcggagggc gcagcggcag
      241 cctcggtggc agcggaggcg gcacggcagg gcctgggatg gccctagggg gcgcgctcac
      301 gaggcgcgcg gtcacgcttc gggtgctgct caaagacgag ctgctggagc ccggtgaggg
      361 ggtgctatcc atctactacc tgggcaggaa gtttactggg gacctgcagc tggatggcag
      421 gatcgtgtgg caggaaactg gacaagtctt caactcaccc agcgcctggg ccacacactg
      481 caagaagctc gtcaacccag ccaagaaatc tggctgtggc tgggcctctg tcaagtacaa
      541 gggccagaag ctggacaaat acaaggccgc ctggcttcgc cggcaccagc tacatatgga
      601 ttacaaggat gacgatgaca agtgactcga gcaccaccac cacnaccact gagatccggc
      661 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
      721 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
      781 atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
      841 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
      901 ttnccttcct ttctcgccac gttcgccggc tttccccgtc aagctctnaa tcgggggctc
      961 cctttag
//
