LOCUS       sequence-211377-        3817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Omura M, Grosmaitre X, Ma M, Mombaerts P
  TITLE     The beta2-adrenergic receptor as a surrogate odorant receptor in 
            mouse olfactory sensory neurons.
  JOURNAL   Mol Cell Neurosci. 2014 Jan;58:1-10. doi: 10.1016/j.mcn.2013.10.010.
            Epub 2013 Nov 6.
  PUBMED    24211702
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2014-01"; volume: "58:1-10. doi"; pages: " 
            10.1016/j.mcn.2013.10.010. Epub 2013 Nov 6"
COMMENT     Sequence Label: pISH-Drd2
FEATURES             Location/Qualifiers
     source          1..3817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      14..602
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     503..522
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     756..773
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    890..911
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(926..931,932..949,950..956)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    964..980
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     969..991
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     988..1004
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     988..1004
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1022..1040
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1022..1039
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1936..1945
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1940..1949
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1979..1998)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1980..1998)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2005..2022)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2005..2021)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2162..2617
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2249..2268)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2459..2480
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2695..2799
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2800..2868,2869..3660)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3018..3037)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
       61 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      121 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      181 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      241 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      301 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      361 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      421 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      481 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      541 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      601 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      661 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      721 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      781 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      841 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      901 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      961 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     1021 tatttaggtg acactataga atactcaagc tatgcatcca acgcgttggg agctctccca
     1081 tatggtcgac ctgcaggcgg ccgcgaattc actagtgatt acacgctaca gctccaagcg
     1141 ccgagttact gtcatgatcg ccattgtctg ggtcctgtcc ttcaccatct cttgcccact
     1201 gctctttgga ctcaacaaca cagaccagaa tgagtgtatc attgccaacc ctgccttcgt
     1261 ggtctactcc tccatcgtct cgttctacgt gcccttcatc gtcaccctgc tggtctatat
     1321 caaaatctac atcgttctcc gcaagcgtcg gaagcgggtc aacaccaagc gtagcagccg
     1381 agctttcaga gccaacctga agacaccact caagggcaac tgtacccacc ctgaggacat
     1441 gaaactctgc accgttatca tgaagtctaa tgggagtttc ccagtgaaca ggcggagaat
     1501 ggatgctgcc cgccgagctc aggagctgga aatggagatg ctgtcaagca ccagcccccc
     1561 agagaggacc cggtatagcc ccatccctcc cagtcaccac cagctcactc tccccgatcc
     1621 atcccaccac ggtctacata gcaaccctga cagtcctgcc aaaccagaaa agaatgggca
     1681 tgccaagatt gtcaatccca ggattgccaa gttctttgag atccagacca tgcccaatgg
     1741 caaaacccgg acctccctta agacgatgag ccgcaggaag ctctcccagc agaaggagaa
     1801 gaaagccact cagatgcttg ccattgttct tggtgtgttc atcatctgct ggctgccctt
     1861 cttcatcacg cacatcctga atatacactg tgactgcaac atcccaccag tcctctacag
     1921 aatcgaattc ccgcggccgc catggcggcc gggagcatgc gacgtcgggc ccaattcgcc
     1981 ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgag aatgggaaaa
     2041 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     2101 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     2161 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     2221 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     2281 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     2341 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     2401 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     2461 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     2521 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     2581 aacgcgaatt ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta
     2641 cgcatctgtg cggtatttca caccgcatca ggtggcactt ttcggggaaa tgtgcgcgga
     2701 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     2761 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     2821 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     2881 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     2941 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     3001 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     3061 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     3121 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     3181 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     3241 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     3301 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     3361 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     3421 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     3481 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     3541 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     3601 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     3661 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     3721 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     3781 ttttcgttcc actgagcgtc agaccccgta gaaaaga
//
