LOCUS       sequence-211387-        9695 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses miR-205.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9695)
  TITLE     van Oers mIR plasmids
REFERENCE   2  (bases 1 to 9695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGC-U6-miR-205
FEATURES             Location/Qualifiers
     source          1..9695
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     ncRNA           63..156
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           854..966
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        1036..2247
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2084..2103
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     primer_bind     complement(2295..2311)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2296..2312)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             join(2314..2316,2317..2319,2320..3033)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2359..2380)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2620..2639)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2967..2988
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             3302..3771
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4471..4488)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4471..4487)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4480..4502)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4696..4715)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4815..4837
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4875..4893)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4961..5065
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5066..5134,5135..5926)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5284..5303)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6097..6685
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6586..6605
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6839..6856
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6973..6994
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7009..7014,7015..7032,7033..7039)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7047..7063
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7052..7074
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7071..7087
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7071..7087
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             7613..8082
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    8147..8504
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             8577..8993
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     8923..8945
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     8961..8977
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     9307..9323
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     promoter        9353..9371
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        join(9455..9695,1..8)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     9455..9475
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     9626..9645
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gaaacaccgt gctcgcttcg gcagcacata tactagtcga ctagggataa cagggtaatt
       61 gtttgaatga ggcttcagta ctttacagaa tcgttgcctg cacatcttgg aaacacttgc
      121 tgggattact tcttcaggtt aacccaacag aaggctcgat aattgaattc gcccttcccg
      181 ggctaggtat acagaatgga gactccactt ggatttatct gggatggccc ttcatgggca
      241 agaaactacc tagagtccag ccccacacac aacattggct ataccaccca gagtactctg
      301 aggtgggaac atgaatgctt gagggtttga gtattcaggt cctcaccagg tcctgaatga
      361 atgaatacag gtattcaggt cctgtaagag tggggttgca ggatcctgaa ggtctccttc
      421 aggtcacaca gcactacacg aggaggctta gtagacaaac agccccagac aatccatggg
      481 tcctcttgtc cttcattcca ccggagtctg tcttatgcca accagatttc agtggagtga
      541 agctcaggag gcatggagct gccagcggtg gactgaagca cagcatttga acagaggcat
      601 tctgcaaaaa tacagtcact aaaagcacct gcagcgtgtg cttttgcaga ggggtggcag
      661 tggcaggcaa gagcacactg ccagtgattg ctgtgtggcc tttcttccta aaaccaatgc
      721 ttcggaaatg tgtatttttc tgtaacttgc aaaattaaaa gttgcctatc tgctgacacg
      781 ccctcctgcc tctctgccct atgttatcag tatcttaaac ctgcaccctg caggcttaaa
      841 tcccagataa gaattcaagg ggctacttta ggagcaatta tcttgtttac taaaactgaa
      901 taccttgcta tctctttgat acatttttac aaagctgaat taaaatggta taaattaaat
      961 cacttttttc aattggaaga ctaatgcggc ctcgacattg attattgact agtaacccgt
     1021 gtcggctcca gatctggcct ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc
     1081 tcacggcgag cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc
     1141 ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc cagtatcagc
     1201 agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc tttccagaga
     1261 gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga tctccgtggg
     1321 gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta gttccgtcgc
     1381 agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta
     1441 gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg ggacggaagc
     1501 gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc cctgaactgg
     1561 gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg aagacgcttg
     1621 tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg caagaaccca
     1681 aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg ggctggggca
     1741 ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt tgtcgtctgt
     1801 tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg
     1861 cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg gtggggccac
     1921 ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc gggcctaggg
     1981 taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata agtgaggcgt
     2041 cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct ccggttttga
     2101 actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg
     2161 taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc
     2221 tggccgtttt tggctttttt gttagacgaa gcttgggctg caggtcgagc tcaagcttcg
     2281 gatcctgcag tcgacgatac cgtcgacctc gacatggtga gcaagggcga ggagctgttc
     2341 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     2401 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     2461 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcac ctacggcgtg
     2521 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     2581 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     2641 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     2701 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     2761 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     2821 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     2881 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     2941 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     3001 atcactctcg gcatggacga gctgtacaag taaggatccg gattagtcca atttgttaaa
     3061 gacaggatat cagtggtcca ggctctagtt ttgactcaac aatatcacca gctgaagcct
     3121 atagagtacg agccatagat aaaataaaag attttattta gtctccagaa aaagggggga
     3181 atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca
     3241 tggaaaaata cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca
     3301 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     3361 agaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
     3421 cccggctcag ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta
     3481 gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt
     3541 gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa
     3601 taaaagagcc cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg
     3661 gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc
     3721 ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc acatgcagca
     3781 tgtatcaaaa ttaatttggt tttttttctt aagtatttac attaaatggc catagtactt
     3841 aaagttacat tggcttcctt gaaataaaca tggagtattc agaatgtgtc ataaatattt
     3901 ctaattttaa gatagtatct ccattggctt tctacttttt cttttatttt tttttgtcct
     3961 ctgtcttcca tttgttgttg ttgttgtttg tttgtttgtt tgttggttgg ttggttaatt
     4021 tttttttaaa gatcctacac tatagttcaa gctagactat tagctactct gtaacccagg
     4081 gtgaccttga agtcatgggt agcctgctgt tttagccttc ccacatctaa gattacaggt
     4141 atgagctatc atttttggta tattgattga ttgattgatt gatgtgtgtg tgtgtgattg
     4201 tgtttgtgtg tgtgattgtg tatatgtgtg tatggttgtg tgtgattgtg tgtatgtatg
     4261 tttgtgtgtg attgtgtgtg tgtgattgtg catgtgtgtg tgtgtgattg tgtttatgtg
     4321 tatgattgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgttgtgtat
     4381 atatatttat ggtagtgaga ggcaacgctc cggctcaggt gtcaggttgg tttttgagac
     4441 agagtctttc acttagcttg gaattaattc actggccgtc gttttacaac gtcgtgactg
     4501 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     4561 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     4621 cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     4681 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     4741 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     4801 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     4861 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     4921 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     4981 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     5041 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     5101 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     5161 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     5221 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     5281 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     5341 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     5401 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     5461 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     5521 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     5581 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     5641 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     5701 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     5761 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     5821 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     5881 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     5941 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     6001 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6061 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6121 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     6181 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6241 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6301 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6361 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6421 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6481 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6541 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6601 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6661 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6721 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     6781 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     6841 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     6901 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     6961 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     7021 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     7081 ctatgaccat gattacgcca agctttgctc ttaggagttt cctaatacat cccaaactca
     7141 aatatataaa gcatttgact tgttctatgc cctagggggc ggggggaagc taagccagct
     7201 ttttttaaca tttaaaatgt taattccatt ttaaatgcac agatgttttt atttcataag
     7261 ggtttcaatg tgcatgaatg ctgcaatatt cctgttacca aagctagtat aaataaaaat
     7321 agataaacgt ggaaattact tagagtttct gtcattaacg tttccttcct cagttgacaa
     7381 cataaatgcg ctgctgagaa gccagtttgc atctgtcagg atcaatttcc cattatgcca
     7441 gtcatattaa ttactagtca attagttgat ttttattttt gacatataca tgtgaaagac
     7501 cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat
     7561 acataactga gaatagaaaa gttcagatca aggtcaggaa cagatggaac agctgaatat
     7621 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
     7681 ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
     7741 gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat
     7801 cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc
     7861 aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc
     7921 ccacaacccc tcactcggcg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg
     7981 tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg
     8041 tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg
     8101 atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa
     8161 cttatctgtg tctgtccgat tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt
     8221 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttcggaa
     8281 cacccggccg caaccctggg agacgtccca gggacttcgg gggccgtttt tgtggcccga
     8341 cctgagtcca aaaatcccga tcgttttgga ctctttggtg cacccccctt agaggaggga
     8401 tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg
     8461 ctttcggttt gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt
     8521 tgtctctgtc tgactgtgtt tctgtatttg tctgaaaata tgggcccgcc gcggcgggcc
     8581 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     8641 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     8701 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     8761 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     8821 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     8881 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     8941 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat
     9001 gagatcttat atggggcacc cccgcccctt gtaaacttcc ctgaccctga catgacaaga
     9061 gttactaaca gcccctctct ccaagctcac ttacaggctc tctacttagt ccagcacgaa
     9121 gtctggagac ctctggcggc agcctaccaa gaacaactgg accgaccggt ggtacctcac
     9181 ccttaccgag tcggcgacac agtgtgggtc cgccgacacc agactaagaa cctagaacct
     9241 cgctggaaag gaccttacac agtcctgctg accaccccca ccgccctcaa agtagacggc
     9301 atcgcagctt ggatacacgc cgcccacgta aaacaattga agatctagtc gaaattaacc
     9361 ctcactaaag ggaacaaaag ctggagctcc accgcggtgg cggccgtgac ggagcgtgac
     9421 cgcgcgccga gcgcgcgcca gggtcgggca ggaagagggc ctatttccca tgattccttc
     9481 atatttgcat atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa
     9541 cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc
     9601 agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt
     9661 cgatttcttg gctttatata tcttgtggaa aggac
//
