LOCUS       sequence-211390-        7290 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Arabinose inducible VioB-mCherry expression (stronger RBS). Used to 
            test burden response in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7290)
  AUTHORS   Ceroni F, Boo A, Furini S, Gorochowski TE, Borkowski O, Ladak YN, 
            Awan AR, Gilbert C, Stan GB, Ellis T
  TITLE     Burden-driven feedback control of gene expression.
  JOURNAL   Nat Methods. 2018 Mar 26. pii: nmeth.4635. doi: 10.1038/nmeth.4635.
  PUBMED    29578536
REFERENCE   2  (bases 1 to 7290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2018 Mar 26. pii: nmeth.4635. doi: 10.1038/nmeth.4635."
COMMENT     Sequence Label:  pLys-M1
FEATURES             Location/Qualifiers
     source          1..7290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(17..22,23..39,40..45))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      157..702
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     603..622
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     819..836
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1228..1330
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1331..1990
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1397..1416)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             complement(2307..3185)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        3212..3496
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     3419..3438
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(6741..6759)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7009..7028
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtagataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattacaa
       61 cttatatcgt atggggctga cttcaggtgc tacatttgaa gagataaatt gcactgaaat
      121 ctagaaatat tttatctgat taataagatg atcttcttga gatcgttttg gtctgcgcgt
      181 aatctcttgc tctgaaaacg aaaaaaccgc cttgcagggc ggtttttcga aggttctctg
      241 agctaccaac tctttgaacc gaggtaactg gcttggagga gcgcagtcac caaaacttgt
      301 cctttcagtt tagccttaac cggcgcatga cttcaagact aactcctcta aatcaattac
      361 cagtggctgc tgccagtggt gcttttgcat gtctttccgg gttggactca agacgatagt
      421 taccggataa ggcgcagcgg tcggactgaa cggggggttc gtgcatacag tccagcttgg
      481 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg cggccataac
      541 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccgcca
      601 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt
      661 cagatttcgt gatgcttgtc aggggggcgg agcctatgga aaaacggctt tgccgcggcc
      721 ctctcacttc cctgttaagt atcttcctgg catcttccag gaaatctccg ccccgttcgt
      781 aagccatttc cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag
      841 cggaatatat cctgtatcac atattctgct gacgcaccgg tgcagccttt tttctcctgc
      901 cacatgaagc acttcactga caccctcatc agtgccaaca tagtaagcca gtatacactc
      961 cgctagcgct gatgtccggc ggtgcttttg ccgttacgca ccaccccgtc agtagctgaa
     1021 caggagggac agctgataga aacagaagcc actggagcac ctcaaaaaca ccatcataca
     1081 ctaaatcagt aagttggcag catcacccga cgcactttgc gccgaataaa tacctgtgac
     1141 ggaagatcac ttcgcagaat aaataaatcc tggtgtccct gttgataccg ggaagccctg
     1201 ggccaacttt tggcgaaaat gagacgttga tcggcacgta agaggttcca actttcacca
     1261 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
     1321 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
     1381 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
     1441 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
     1501 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
     1561 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
     1621 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
     1681 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
     1741 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
     1801 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
     1861 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1921 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
     1981 cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctggtt gctacgcctg
     2041 aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga cccggtcgtc
     2101 ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac cggtttattg
     2161 actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt tgctcaggct
     2221 ctccccgtgg aggtaataat tgacgatatg atcatttatt ctgcctccca gagcctgata
     2281 aactctagaa gcggccgctt ctagagttat gacaacttga cggctacatc attcactttt
     2341 tcttcacaac cggcacggaa ctcgctcggg ctggccccgg tgcatttttt aaatacccgc
     2401 gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg
     2461 gtggtgctca aaagcagctt cgcctggctg atacgttggt cctcgcgcca gcttaagacg
     2521 ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc
     2581 tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat gtactgacaa
     2641 gcctcgcgta cccgattatc catcggtgga tggagcgact cgttaatcgc ttccatgcgc
     2701 cgcagtaaca attgctcaag cagatttatc gccagcagct ccgaatagcg cccttcccct
     2761 tgcccggcgt taatgatttg cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc
     2821 gggcgaaaga accccgtatt ggcaaatatt gacggccagt taagccattc atgccagtag
     2881 gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg atgacgaccg
     2941 tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca aacaaattct
     3001 cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat ataacctttc
     3061 attcccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa
     3121 cccgccacca gatgggcatt aaacgagtat cccggcagca ggggatcatt ttgcgcttca
     3181 gccatacttt tcatactccc gccattcaga gaagaaacca attgtccata ttgcatcaga
     3241 cattgccgtc actgcgtctt ttactggctc ttctcgctaa ccaaaccggt aaccccgctt
     3301 attaaaagca ttctgtaaca aagcgggacc aaagccatga caaaaacgcg taacaaaagt
     3361 gtctataatc acggcagaaa agtccacatt gattatttgc acggcgtcac actttgctat
     3421 gccatagcat ttttatccat aagattagcg gatcctacct gacacttttt atcgcaactc
     3481 tctataattt ctccataccc gtttttttgg gctagcaggc atctttcaac taaagtaaga
     3541 ggtaataatt atgagcattc tggatttccc gcgtatccac ttccgtggct gggcccgtgt
     3601 caatgcgccg accgcgaacc gcgatccgca cggccacatc gatatggcca gcaataccgt
     3661 ggcgatggcg ggtgagccgt tcgacctggc acgccatcct acggagttcc accgtcacct
     3721 gcgctccctg ggtccgcgct tcggcttgga tggtcgtgct gacccggaag gcccgttcag
     3781 cctggccgag ggctacaacg ctgccggtaa caaccacttt tcgtgggaga gcgcaaccgt
     3841 tagccacgtg caatgggatg gcggtgaggc ggatcgtggt gacggtctgg tcggtgctcg
     3901 tttggcactg tggggtcact acaatgatta tctgcgtacc accttcaatc gtgctcgttg
     3961 ggtcgacagc gacccgacgc gccgtgacgc tgcacaaatc tatgcgggcc aattcaccat
     4021 tagcccggct ggtgccggtc cgggtacgcc gtggctgttt acggcagaca ttgatgatag
     4081 ccatggtgca cgttggacgc gtggcggcca cattgcagag cgtggcggcc acttcttgga
     4141 tgaagagttt ggtctggcac gcctgtttca gttctctgtg ccgaaagatc acccacattt
     4201 tctgtttcac ccgggtccgt ttgattccga ggcctggcgt cgtctgcaat tggctctgga
     4261 ggatgacgac gttctgggtc tgaccgtgca atatgcgttg ttcaatatga gcaccccgcc
     4321 tcagccgaac agcccggttt ttcacgatat ggtcggtgtt gtcggtctgt ggcgtcgtgg
     4381 tgaactggcg agctacccgg ctggtcgtct gctgcgtccg cgtcaaccgg gtctgggtga
     4441 cctgaccctg cgcgtcaacg gtggtcgcgt tgcgctgaat ttggcgtgtg ccattccgtt
     4501 cagcactcgt gccgcgcagc caagcgcacc ggaccgcctg accccggacc tgggtgccaa
     4561 actgccgctg ggcgatctgc tgctgcgtga tgaggacggc gcactgttgg cacgtgtgcc
     4621 gcaggctctg taccaagact attggacgaa tcacggtatt gtggacctgc cgctgctgcg
     4681 cgaaccgcgt ggtagcttga ccctgagcag cgaactggcg gagtggcgtg agcaagactg
     4741 ggtcacccaa agcgacgcgt ctaacctgta cctggaggca ccggatcgcc gtcacggtcg
     4801 ctttttccct gagagcatcg cgctgcgcag ctactttcgc ggtgaagcgc gtgcgcgtcc
     4861 ggatatcccg catcgtatcg agggcatggg cctggtcggc gtcgaatctc gtcaggatgg
     4921 cgacgctgcg gaatggcgtc tgacgggtct gcgtccgggt ccggcacgca ttgttctgga
     4981 cgatggtgcc gaggcgatcc ctctgcgtgt tctgcctgac gattgggcgc tggatgacgc
     5041 gaccgtcgaa gaagtggatt acgccttttt gtaccgccac gttatggcgt attacgagct
     5101 ggtgtatcca ttcatgagcg acaaggtgtt ttccctggct gatcgttgca aatgtgaaac
     5161 gtacgcacgt ctgatgtggc agatgtgtga tccgcagaac cgcaacaagt cctattacat
     5221 gccgagcacc cgcgaactgt cggcaccgaa agctcgtttg ttcttgaagt atctggccca
     5281 cgtggaaggc caggcacgcc tgcaagcacc tccgccagcg ggtccggcac gcattgaatc
     5341 taaagcccag ttggcggcag agctgcgtaa agccgtcgac ctggagctgt ctgtgatgct
     5401 gcaatacctg tacgcggcgt atagcattcc gaactatgca cagggccaac aacgtgttcg
     5461 tgacggtgcg tggaccgccg agcagctgca actggcgtgc ggtagcggtg accgtcgccg
     5521 tgatggcggt attcgtgcag cactgctgga aattgctcat gaagaaatga ttcattacct
     5581 ggtcgttaac aacctgctga tggccctggg cgagccgttc tacgcgggtg tcccgctgat
     5641 gggcgaagcg gcacgtcagg cgtttggcct ggacaccgag ttcgctctgg aaccgtttag
     5701 cgaaagcacg ctggcacgtt ttgttcgtct ggaatggccg cactttatcc cagcaccggg
     5761 caaatccatc gcggactgct atgccgccat tcgtcaggcg tttttggatc tgccggactt
     5821 gtttggtggc gaggcaggta agcgtggcgg tgaacaccac ctgttcctga atgagctgac
     5881 caaccgtgcg catccgggtt atcaactgga agttttcgat cgcgactcgg cgctgtttgg
     5941 tattgcattt gtgaccgatc agggcgaagg tggcgctctg gacagcccgc actacgaaca
     6001 tagccatttt caacgtctgc gtgaaatgag cgcgcgtatc atggctcaaa gcgcaccgtt
     6061 cgaaccggcg ctgccggcgt tgcgtaatcc ggttctggat gagagcccgg gttgccaacg
     6121 tgtcgcagac ggtcgtgcgc gtgcgctgat ggcattgtac caaggcgttt atgagctgat
     6181 gtttgcgatg atggcgcagc acttcgccgt gaaaccgctg ggtagcttgc gtcgcagccg
     6241 cctgatgaac gcagcaatcg atctgatgac cggtctgttg cgtccgctga gctgcgcgct
     6301 gatgaacctg ccaagcggca tcgccggtcg cacggccggt ccgccgctgc cgggtccggt
     6361 tgacacccgt agctatgacg actacgcgct gggctgtcgc atgctggcac gccgttgcga
     6421 gcgtctgctg gagcaggcga gcatgctgga accgggttgg ctgccggatg cgcagatgga
     6481 gctgctggat ttctatcgtc gccaaatgct ggacttggcg tgcggcaaac tgagccgcga
     6541 ggccgccgaa gctgcagcaa aggaggccgc agctaaagag gctgcagcga aagcgatggt
     6601 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     6661 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     6721 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     6781 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     6841 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     6901 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccttgc aggacggcga
     6961 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     7021 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     7081 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     7141 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     7201 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     7261 cgagggccgc cactccacct aataatacta
//
