LOCUS       sequence-211392-        2303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty plasmid of the pLys-M1 construct. This is medium copy (p15A), 
            CmR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ceroni F, Boo A, Furini S, Gorochowski TE, Borkowski O, Ladak YN, 
            Awan AR, Gilbert C, Stan GB, Ellis T
  TITLE     Burden-driven feedback control of gene expression.
  JOURNAL   Nat Methods. 2018 Mar 26. pii: nmeth.4635. doi: 10.1038/nmeth.4635.
  PUBMED    29578536
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2018 Mar 26. pii: nmeth.4635. doi: 10.1038/nmeth.4635."
COMMENT     Sequence Label: pLys-M1 backbone
FEATURES             Location/Qualifiers
     source          1..2303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(22..27,28..44,45..50))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      162..707
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     608..627
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     824..841
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1233..1335
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1336..1995
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1402..1421)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tactagtaga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat
       61 tacaacttat atcgtatggg gctgacttca ggtgctacat ttgaagagat aaattgcact
      121 gaaatctaga aatattttat ctgattaata agatgatctt cttgagatcg ttttggtctg
      181 cgcgtaatct cttgctctga aaacgaaaaa accgccttgc agggcggttt ttcgaaggtt
      241 ctctgagcta ccaactcttt gaaccgaggt aactggcttg gaggagcgca gtcaccaaaa
      301 cttgtccttt cagtttagcc ttaaccggcg catgacttca agactaactc ctctaaatca
      361 attaccagtg gctgctgcca gtggtgcttt tgcatgtctt tccgggttgg actcaagacg
      421 atagttaccg gataaggcgc agcggtcgga ctgaacgggg ggttcgtgca tacagtccag
      481 cttggagcga actgcctacc cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc
      541 ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc
      601 cgccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac cactgatttg
      661 agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggctttgccg
      721 cggccctctc acttccctgt taagtatctt cctggcatct tccaggaaat ctccgccccg
      781 ttcgtaagcc atttccgctc gccgcagtcg aacgaccgag cgtagcgagt cagtgagcga
      841 ggaagcggaa tatatcctgt atcacatatt ctgctgacgc accggtgcag ccttttttct
      901 cctgccacat gaagcacttc actgacaccc tcatcagtgc caacatagta agccagtata
      961 cactccgcta gcgctgatgt ccggcggtgc ttttgccgtt acgcaccacc ccgtcagtag
     1021 ctgaacagga gggacagctg atagaaacag aagccactgg agcacctcaa aaacaccatc
     1081 atacactaaa tcagtaagtt ggcagcatca cccgacgcac tttgcgccga ataaatacct
     1141 gtgacggaag atcacttcgc agaataaata aatcctggtg tccctgttga taccgggaag
     1201 ccctgggcca acttttggcg aaaatgagac gttgatcggc acgtaagagg ttccaacttt
     1261 caccataatg aaataagatc actaccgggc gtattttttg agttatcgag attttcagga
     1321 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
     1381 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
     1441 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
     1501 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
     1561 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
     1621 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
     1681 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
     1741 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
     1801 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
     1861 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
     1921 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
     1981 cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggttgctac
     2041 gcctgaataa gtgataataa gcggatgaat ggcagaaatt cgaaagcaaa ttcgacccgg
     2101 tcgtcggttc agggcagggt cgttaaatag ccgcttatgt ctattgctgg tttaccggtt
     2161 tattgactac cggaagcagt gtgaccgtgt gcttctcaaa tgcctgaggc cagtttgctc
     2221 aggctctccc cgtggaggta ataattgacg atatgatcat ttattctgcc tcccagagcc
     2281 tgataaactc tagaagcggc cgc
//
