LOCUS       sequence-211395-        1689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  (+)-Limonene Synthase from Citrus sinensis in pET-28a(+). N-terminal
            was truncated to remove amino acids 1-52 and then His-tagged for 
            purification..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Morehouse BR, Kumar RP, Matos JO, Olsen SN, Entova S, Oprian DD
  TITLE     Functional and Structural Characterization of a (+)-Limonene 
            Synthase from Citrus sinensis.
  JOURNAL   Biochemistry. 2017 Mar 28;56(12):1706-1715. doi: 
            10.1021/acs.biochem.7b00143. Epub 2017 Mar 15.
  PUBMED    28272875
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.7b00143"; journalName: "Biochemistry"; date: 
            "2017-03-28- 28"; volume: "56"; issue: "12"; pages: "1706-1715"
COMMENT     Sequence Label: (+)-Limonene Synthase
FEATURES             Location/Qualifiers
     source          1..1689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcatcatc atcatcatca taggagatca gcaaactacc aaccttcaat ttgggaccat
       61 gattttttgc agtcactgaa tagcaactat acggatgaaa catacaaaag acgagcagaa
      121 gagctgaagg gaaaagtgaa gacagcgatt aaggatgtaa ccgagcctct ggatcagttg
      181 gagctgattg ataatttgca aagacttgga ttggcttatc attttgagcc tgagattcgg
      241 aacatattgc gtaatatcca caaccataat aaagattata attggagaaa agaaaatctg
      301 tatgcaacct cccttgaatt cagactactt agacaacatg gctatcctgt ttctcaagag
      361 gttttcagtg gttttaaaga cgacaaggta ggcttcattt gtgatgattt caagggaata
      421 ctgagcttgc atgaagcctc gtattacagc ttagaaggag aaagcatcat ggaggaggcc
      481 tggcaattca ccagtaagca tcttaaagaa atgatgatca ccagcaacag caaggaagag
      541 gatgtatttg tagcagaaca agcgaagcgg gcgctggagc tccctctgca ttggaaagcg
      601 cctatgttag aggcaaggtg gttcatacac gtttatgaga aaagagagga caagaaccac
      661 cttttacttg agctcgctaa gttggagttt aacactttgc aggcaattta ccaggaagaa
      721 cttaaagaca tttcagggtg gtggaaggat acaggtcttg gagagaaatt gagctttgcg
      781 aggaacaggt tggtagcgtc cttcttatgg agcatgggga tcgcgtttga gcctcaattc
      841 gcctactgca ggagagtgct cacaatctcg atagccctaa ttacagtgat tgatgacatt
      901 tatgatgtct atggaacatt ggatgaactt gagatattca ctgatgctgt tgcgaggtgg
      961 gacatcaatt atgccttgaa gcaccttccg ggctatatga aaatgtgttt tcttgccctt
     1021 tacaactttg ttaatgaatt tgcttattac gttctcaaac aacaggattt tgatatgctt
     1081 ctgagcataa aacatgcatg gcttggctta atacaagcct acttggtgga ggcgaaatgg
     1141 taccatagca agtacacacc gaaactggaa gaatacttgg aaaatggatt ggtatcaata
     1201 acgggccctt taattataac gatttcatat ctttctggta caaatccaat cattaagaag
     1261 gaactggaat ttctagaaag taatccagat atagttcact ggtcatccaa gattttccgt
     1321 ctgcaagatg atttgggaac ttcatcggac gagatacaga gaggggatgt tccgaaatca
     1381 atccagtgtt acatgcatga aactggtgcc tcggaggaag ttgctcgtga acacatcaag
     1441 gatatgatga gacagatgtg gaagaaggtg aatgcataca cagccgataa agactctccc
     1501 ttgactcgaa caactgctga gttcctcttg aatcttgtgc gaatgtccca ttttatgtat
     1561 ctacatggag atgggcatgg tgttcaaaac caagagacta tcgatgtcgg ctttacattg
     1621 ctttttcagc ccattccctt ggaggacaaa gacatggctt tcacagcatc tcctggcacc
     1681 aaaggctga
//
