LOCUS       sequence-211401-       12106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of BE3 (not codon 
            optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12106)
  AUTHORS   Zafra MP, Schatoff EM, Katti A, Foronda M, Breinig M, Schweitzer AY,
            Simon A, Han T, Goswami S, Montgomery E, Thibado J, Kastenhuber ER, 
            Sanchez-Rivera FJ, Shi J, Vakoc CR, Lowe SW, Tschaharganeh DF, Dow 
            LE
  TITLE     Optimized base editors enable efficient editing in cells, organoids 
            and mice.
  JOURNAL   Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
  PUBMED    29969439
REFERENCE   2  (bases 1 to 12106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194."
COMMENT     Sequence Label: pLenti-BE3-P2A-Puro
FEATURES             Location/Qualifiers
     source          1..12106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        11..237
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             238..418
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    465..590
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1083..1316
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1501..1545
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1729..1846
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1915..2126
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2152..2838
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             join(2887..6285,6286..6288,6289..6885,6886,6887..6889,
                     6890..6891,6892..6894,6895..6987)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(2887..2910,2911..2913,2914..6987)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7000..7248
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7261..7281
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7297..7353
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7354..7953
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7354..7373)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7850..7870
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7969..8557
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8022..8042)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8440..8451)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8629..8862
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8940..9061
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8977..8996)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9031..9050
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9101..9236
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9256..9275)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9257..9275)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9285..9302)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9285..9301)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9294..9316)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9443..9898
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9530..9549)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9740..9761
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9924..10028
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10029..10097,10098..10889)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10247..10266)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11060..11648
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11549..11568
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11802..11819
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11936..11957
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11972..11977,11978..11995,11996..12002)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12010..12026
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12015..12037
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12034..12050
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12034..12050
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12069..12089
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12071..12089
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
       61 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
      121 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
      181 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
      241 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      301 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      361 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      421 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      481 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      541 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      601 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
      661 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
      721 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
      781 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
      841 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
      901 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
      961 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1021 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1081 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1141 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1201 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1261 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1321 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1381 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1441 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1501 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1561 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1621 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     1681 ttatcgtttc agacccacct cccaaccccg aggggaccca gaccacaatt ttaaaagaaa
     1741 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     1801 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     1861 ggacagcaga gatccacttt ggcgccggct cgagtggctc cggtgcccgt cagtgggcag
     1921 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
     1981 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     2041 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     2101 gcaacgggtt tgccgccaga acacaggtgt cgtgacgcgg gatccgccac catgagctca
     2161 gagactggcc cagtggctgt ggaccccaca ttgagacggc ggatcgagcc ccatgagttt
     2221 gaggtattct tcgatccgag agagctccgc aaggagacct gcctgcttta cgaaattaat
     2281 tgggggggcc ggcactccat ttggcgacat acatcacaga acactaacaa gcacgtcgaa
     2341 gtcaacttca tcgagaagtt cacgacagaa agatatttct gtccgaacac aaggtgcagc
     2401 attacctggt ttctcagctg gagcccatgc ggcgaatgta gtagggccat cactgaattc
     2461 ctgtcaaggt atccccacgt cactctgttt atttacatcg caaggctgta ccaccacgct
     2521 gacccccgca atcgacaagg cctgcgggat ttgatctctt caggtgtgac tatccaaatt
     2581 atgactgagc aggagtcagg atactgctgg agaaactttg tgaattatag cccgagtaat
     2641 gaagcccact ggcctaggta tccccatctg tgggtacgac tgtacgttct tgaactgtac
     2701 tgcatcatac tgggcctgcc tccttgtctc aacattctga gaaggaagca gccacagctg
     2761 acattcttta ccatcgctct tcagtcttgt cattaccagc gactgccccc acacattctc
     2821 tgggccaccg ggttgaaaag cggcagcgag actcccggga cctcagagtc cgccacaccc
     2881 gaaagtgata aaaagtattc tattggttta gccatcggca ctaattccgt tggatgggct
     2941 gtcataaccg atgaatacaa agtaccttca aagaaattta aggtgttggg gaacacagac
     3001 cgtcattcga ttaaaaagaa tcttatcggt gccctcctat tcgatagtgg cgaaacggca
     3061 gaggcgactc gcctgaaacg aaccgctcgg agaaggtata cacgtcgcaa gaaccgaata
     3121 tgttacttac aagaaatttt tagcaatgag atggccaaag ttgacgattc tttctttcac
     3181 cgtttggaag agtccttcct tgtcgaagag gacaagaaac atgaacggca ccccatcttt
     3241 ggaaacatag tagatgaggt ggcatatcat gaaaagtacc caacgattta tcacctcaga
     3301 aaaaagctag ttgactcaac tgataaagcg gacctgaggt taatctactt ggctcttgcc
     3361 catatgataa agttccgtgg gcactttctc attgagggtg atctaaatcc ggacaactcg
     3421 gatgtcgaca aactgttcat ccagttagta caaacctata atcagttgtt tgaagagaac
     3481 cctataaatg caagtggcgt ggatgcgaag gctattctta gcgcccgcct ctctaaatcc
     3541 cgacggctag aaaacctgat cgcacaatta cccggagaga agaaaaatgg gttgttcggt
     3601 aaccttatag cgctctcact aggcctgaca ccaaatttta agtcgaactt cgacttagct
     3661 gaagatgcca aattgcagct tagtaaggac acgtacgatg acgatctcga caatctactg
     3721 gcacaaattg gagatcagta tgcggactta tttttggctg ccaaaaacct tagcgatgca
     3781 atcctcctat ctgacatact gagagttaat actgagatta ccaaggcgcc gttatccgct
     3841 tcaatgatca aaaggtacga tgaacatcac caagacttga cacttctcaa ggccctagtc
     3901 cgtcagcaac tgcctgagaa atataaggaa atattctttg atcagtcgaa aaacgggtac
     3961 gcaggttata ttgacggcgg agcgagtcaa gaggaattct acaagtttat caaacccata
     4021 ttagagaaga tggatgggac ggaagagttg cttgtaaaac tcaatcgcga agatctactg
     4081 cgaaagcagc ggactttcga caacggtagc attccacatc aaatccactt aggcgaattg
     4141 catgctatac ttagaaggca ggaggatttt tatccgttcc tcaaagacaa tcgtgaaaag
     4201 attgagaaaa tcctaacctt tcgcatacct tactatgtgg gacccctggc ccgagggaac
     4261 tctcggttcg catggatgac aagaaagtcc gaagaaacga ttactccatg gaattttgag
     4321 gaagttgtcg ataaaggtgc gtcagctcaa tcgttcatcg agaggatgac caactttgac
     4381 aagaatttac cgaacgaaaa agtattgcct aagcacagtt tactttacga gtatttcaca
     4441 gtgtacaatg aactcacgaa agttaagtat gtcactgagg gcatgcgtaa acccgccttt
     4501 ctaagcggag aacagaagaa agcaatagta gatctgttat tcaagaccaa ccgcaaagtg
     4561 acagttaagc aattgaaaga ggactacttt aagaaaattg aatgcttcga ttctgtcgag
     4621 atctccgggg tagaagatcg atttaatgcg tcacttggta cgtatcatga cctcctaaag
     4681 ataattaaag ataaggactt cctggataac gaagagaatg aagatatctt agaagatata
     4741 gtgttgactc ttaccctctt tgaagatcgg gaaatgattg aggaaagact aaaaacatac
     4801 gctcacctgt tcgacgataa ggttatgaaa cagttaaaga ggcgtcgcta tacgggctgg
     4861 ggacgattgt cgcggaaact tatcaacggg ataagagaca agcaaagtgg taaaactatt
     4921 ctcgattttc taaagagcga cggcttcgcc aataggaact ttatgcagct gatccatgat
     4981 gactctttaa ccttcaaaga ggatatacaa aaggcacagg tttccggaca aggggactca
     5041 ttgcacgaac atattgcgaa tcttgctggt tcgccagcca tcaaaaaggg catactccag
     5101 acagtcaaag tagtggatga gctagttaag gtcatgggac gtcacaaacc ggaaaacatt
     5161 gtaatcgaga tggcacgcga aaatcaaacg actcagaagg ggcaaaaaaa cagtcgagag
     5221 cggatgaaga gaatagaaga gggtattaaa gaactgggca gccagatctt aaaggagcat
     5281 cctgtggaaa atacccaatt gcagaacgag aaactttacc tctattacct acaaaatgga
     5341 agggacatgt atgttgatca ggaactggac ataaaccgtt tatctgatta cgacgtcgat
     5401 cacattgtac cccaatcctt tttgaaggac gattcaatcg acaataaagt gcttacacgc
     5461 tcggataaga accgagggaa aagtgacaat gttccaagcg aggaagtcgt aaagaaaatg
     5521 aagaactatt ggcggcagct cctaaatgcg aaactgataa cgcaaagaaa gttcgataac
     5581 ttaactaaag ctgagagggg tggcttgtct gaacttgaca aggccggatt tattaaacgt
     5641 cagctcgtgg aaacccgcca aatcacaaag catgttgcac agatactaga ttcccgaatg
     5701 aatacgaaat acgacgagaa cgataagctg attcgggaag tcaaagtaat cactttaaag
     5761 tcaaaattgg tgtcggactt cagaaaggat tttcaattct ataaagttag ggagataaat
     5821 aactaccacc atgcgcacga cgcttatctt aatgccgtcg tagggaccgc actcattaag
     5881 aaatacccga agctagaaag tgagtttgtg tatggtgatt acaaagttta tgacgtccgt
     5941 aagatgatcg cgaaaagcga acaggagata ggcaaggcta cagccaaata cttcttttat
     6001 tctaacatta tgaatttctt taagacggaa atcactctgg caaacggaga gatacgcaaa
     6061 cgacctttaa ttgaaaccaa tggggagaca ggtgaaatcg tatgggataa gggccgggac
     6121 ttcgcgacgg tgagaaaagt tttgtccatg ccccaagtca acatagtaaa gaaaactgag
     6181 gtgcagaccg gagggttttc aaaggaatcg attcttccaa aaaggaatag tgataagctc
     6241 atcgctcgta aaaaggactg ggacccgaaa aagtacggtg gcttcgatag ccctacagtt
     6301 gcctattctg tcctagtagt ggcaaaagtt gagaagggaa aatccaagaa actgaagtca
     6361 gtcaaagaat tattggggat aacgattatg gagcgctcgt cttttgaaaa gaaccccatc
     6421 gacttccttg aggcgaaagg ttacaaggaa gtaaaaaagg atctcataat taaactacca
     6481 aagtatagtc tgtttgagtt agaaaatggc cgaaaacgga tgttggctag cgccggagag
     6541 cttcaaaagg ggaacgaact cgcactaccg tctaaatacg tgaatttcct gtatttagcg
     6601 tcccattacg agaagttgaa aggttcacct gaagataacg aacagaagca actttttgtt
     6661 gagcagcaca aacattatct cgacgaaatc atagagcaaa tttcggaatt cagtaagaga
     6721 gtcatcctag ctgatgccaa tctggacaaa gtattaagcg catacaacaa gcacagggat
     6781 aaacccatac gtgagcaggc ggaaaatatt atccatttgt ttactcttac caacctcggc
     6841 gctccagccg cattcaagta ttttgacaca acgatagatc gcaaacgata cacttctacc
     6901 aaggaggtgc tagacgcgac actgattcac caatccatca cgggattata tgaaactcgg
     6961 atagatttgt cacagcttgg gggtgactct ggtggttcta ctaatctgtc agatattatt
     7021 gaaaaggaga ccggtaagca actggttatc caggaatcca tcctcatgct cccagaggag
     7081 gtggaagaag tcattgggaa caagccggaa agcgatatac tcgtgcacac cgcctacgac
     7141 gagagcaccg acgagaatgt catgcttctg actagcgacg cccctgaata caagccttgg
     7201 gctctggtca tacaggatag caacggtgag aacaagatta agatgctctc tggtggttct
     7261 cccaagaaga agaggaaagt cgctagcggc agcggcgcca ccaacttcag cctgctgaag
     7321 caggccggcg acgtggagga gaaccccggc cccatgaccg agtacaagcc cacggtgcgc
     7381 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     7441 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     7501 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     7561 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     7621 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     7681 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcgga
     7741 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     7801 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     7861 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     7921 acctggtgca tgacccgcaa gcccggtgcc tgaacgcgtt aagtcgacaa tcaacctctg
     7981 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     8041 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     8101 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     8161 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     8221 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     8281 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     8341 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     8401 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     8461 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     8521 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
     8581 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     8641 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     8701 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8761 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8821 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat
     8881 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     8941 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     9001 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     9061 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     9121 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     9181 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     9241 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     9301 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     9361 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     9421 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     9481 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     9541 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     9601 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     9661 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     9721 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     9781 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     9841 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     9901 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     9961 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    10021 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    10081 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    10141 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    10201 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    10261 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    10321 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    10381 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    10441 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    10501 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    10561 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    10621 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    10681 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    10741 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    10801 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    10861 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    10921 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    10981 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    11041 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    11101 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    11161 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    11221 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    11281 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    11341 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    11401 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    11461 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    11521 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    11581 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    11641 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    11701 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    11761 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    11821 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    11881 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    11941 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    12001 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
    12061 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagct
//
