LOCUS       sequence-211402-       12106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of BE3RA in mammalian 
            cells (codon optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12106)
  AUTHORS   Zafra MP, Schatoff EM, Katti A, Foronda M, Breinig M, Schweitzer AY,
            Simon A, Han T, Goswami S, Montgomery E, Thibado J, Kastenhuber ER, 
            Sanchez-Rivera FJ, Shi J, Vakoc CR, Lowe SW, Tschaharganeh DF, Dow 
            LE
  TITLE     Optimized base editors enable efficient editing in cells, organoids 
            and mice.
  JOURNAL   Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
  PUBMED    29969439
REFERENCE   2  (bases 1 to 12106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194."
COMMENT     Sequence Label: pLenti-BE3RA-P2A-Puro
FEATURES             Location/Qualifiers
     source          1..12106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..245
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             246..426
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    473..598
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1091..1324
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1509..1553
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1737..1854
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1923..2134
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2160..2846
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             join(2895..2918,2919..2921,2922..6995)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2895..6995
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7008..7256
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7269..7289
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7305..7361
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7362..7961
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7362..7381)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7858..7878
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7977..8565
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8030..8050)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8448..8459)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8637..8870
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8948..9069
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8985..9004)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9039..9058
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9109..9244
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9264..9283)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9265..9283)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9293..9310)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9293..9309)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9302..9324)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9451..9906
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9538..9557)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9748..9769
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9932..10036
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10037..10105,10106..10897)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10255..10274)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11068..11656
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11557..11576
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11810..11827
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11944..11965
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11980..11985,11986..12003,12004..12010)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12018..12034
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12023..12045
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12042..12058
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12042..12058
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12077..12097
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12079..12097
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
       61 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
      121 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
      181 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
      241 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
      301 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
      361 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
      421 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
      481 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
      541 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
      601 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
      661 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
      721 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
      781 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
      841 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
      901 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
      961 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     1021 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     1081 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     1141 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     1201 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     1261 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     1321 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     1381 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     1441 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     1501 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     1561 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     1621 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     1681 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccaga ccacaatttt
     1741 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     1801 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     1861 tattacaggg acagcagaga tccactttgg cgccggctcg agtggctccg gtgcccgtca
     1921 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     1981 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     2041 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     2101 tctttttcgc aacgggtttg ccgccagaac acaggtgtcg tgacgcggga tccgccacca
     2161 tgagctcaga gactggccca gtggctgtgg accccacatt gagacggcgg atcgagcccc
     2221 atgagtttga ggtattcttc gatccgagag agctccgcaa ggagacctgc ctgctttacg
     2281 aaattaattg ggggggccgg cactccattt ggcgacatac atcacagaac actaacaagc
     2341 acgtcgaagt caacttcatc gagaagttca cgacagaaag atatttctgt ccgaacacaa
     2401 ggtgcagcat tacctggttt ctcagctgga gcccatgcgg cgaatgtagt agggccatca
     2461 ctgaattcct gtcaaggtat ccccacgtca ctctgtttat ttacatcgca aggctgtacc
     2521 accacgctga cccccgcaat cgacaaggcc tgcgggattt gatctcttca ggtgtgacta
     2581 tccaaattat gactgagcag gagtcaggat actgctggag aaactttgtg aattatagcc
     2641 cgagtaatga agcccactgg cctaggtatc cccatctgtg ggtacgactg tacgttcttg
     2701 aactgtactg catcatactg ggcctgcctc cttgtctcaa cattctgaga aggaagcagc
     2761 cacagctgac attctttacc atcgctcttc agtcttgtca ttaccagcga ctgcccccac
     2821 acattctctg ggccaccggg ttgaaaagcg gcagcgagac tcccgggacc tcagagtccg
     2881 ccacacccga aagtgacaag aagtacagca tcggcctggc catcggcacc aactctgtgg
     2941 gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca
     3001 acaccgaccg gcacagcatc aagaagaacc tgatcggagc cctgctgttc gacagcggcg
     3061 aaacagccga ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga
     3121 accggatctg ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct
     3181 tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc
     3241 ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc
     3301 acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg
     3361 ccctggccca catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg
     3421 acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg
     3481 aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga
     3541 gcaagagcag acggctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc
     3601 tgttcggcaa cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg
     3661 acctggccga ggatgccaaa ctgcagctga gcaaggacac ctacgacgac gacctggaca
     3721 acctgctggc ccagatcggc gaccagtacg ccgacctgtt tctggccgcc aagaacctgt
     3781 ccgacgccat cctgctgagc gacatcctga gagtgaacac cgagatcacc aaggcccccc
     3841 tgagcgcctc tatgatcaag agatacgacg agcaccacca ggacctgacc ctgctgaaag
     3901 ctctcgtgcg gcagcagctg cctgagaagt acaaagagat tttcttcgac cagagcaaga
     3961 acggctacgc cggctacatt gacggcggag ccagccagga agagttctac aagttcatca
     4021 agcccatcct ggaaaagatg gacggcaccg aggaactgct cgtgaagctg aacagagagg
     4081 acctgctgcg gaagcagcgg accttcgaca acggcagcat cccccaccag atccacctgg
     4141 gagagctgca cgccattctg cggcggcagg aagattttta cccattcctg aaggacaacc
     4201 gggaaaagat cgagaagatc ctgaccttcc gcatccccta ctacgtgggc cctctggcca
     4261 ggggaaacag cagattcgcc tggatgacca gaaagagcga ggaaaccatc accccctgga
     4321 acttcgagga agtggtggac aagggcgctt ccgcccagag cttcatcgag cggatgacca
     4381 acttcgataa gaacctgccc aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt
     4441 acttcaccgt gtataacgag ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc
     4501 ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc
     4561 ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag tgcttcgact
     4621 ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca taccacgatc
     4681 tgctgaaaat tatcaaggac aaggacttcc tggacaatga ggaaaacgag gacattctgg
     4741 aagatatcgt gctgaccctg acactgtttg aggacagaga gatgatcgag gaacggctga
     4801 aaacctatgc ccacctgttc gacgacaaag tgatgaagca gctgaagcgg cggagataca
     4861 ccggctgggg caggctgagc cggaagctga tcaacggcat ccgggacaag cagtccggca
     4921 agacaatcct ggatttcctg aagtccgacg gcttcgccaa cagaaacttc atgcagctga
     4981 tccacgacga cagcctgacc tttaaagagg acatccagaa agcccaggtg tccggccagg
     5041 gcgatagcct gcacgagcac attgccaatc tggccggcag ccccgccatt aagaagggca
     5101 tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg
     5161 agaacatcgt gatcgaaatg gccagagaga accagaccac ccagaaggga cagaagaaca
     5221 gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc cagatcctga
     5281 aagaacaccc agtggaaaac acccagctgc agaacgagaa gctgtacctg tactacctgc
     5341 agaatgggcg ggatatgtac gtggaccagg aactggacat caaccggctg tccgactacg
     5401 atgtggacca tatcgtgcct cagagctttc tgaaggacga ctccatcgac aacaaggtgc
     5461 tgaccagaag cgacaagaac cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga
     5521 agaagatgaa gaactactgg cggcagctgc tgaacgccaa gctgattacc cagagaaagt
     5581 tcgacaatct gaccaaggcc gagagaggcg gcctgagcga actggataag gccggcttca
     5641 tcaagagaca gctggtggaa acccggcaga tcacaaagca cgtggcacag atcctggact
     5701 cccggatgaa cactaagtac gacgagaatg acaagctgat ccgggaagtg aaagtgatca
     5761 ccctgaagtc caagctggtg tccgatttcc ggaaggattt ccagttttac aaagtgcgcg
     5821 agatcaacaa ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc
     5881 tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta cggcgactac aaggtgtacg
     5941 acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg caaggctacc gccaagtact
     6001 tcttctacag caacatcatg aactttttca agaccgagat taccctggcc aacggcgaga
     6061 tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg
     6121 gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa
     6181 agaccgaggt gcagacaggc ggcttcagca aagagtctat cctgcccaag aggaacagcg
     6241 ataagctgat cgccagaaag aaggactggg accctaagaa gtacggcggc ttcgacagcc
     6301 ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac
     6361 tgaagagtgt gaaagagctg ctggggatca ccatcatgga aagaagcagc ttcgagaaga
     6421 atcccatcga ctttctggaa gccaagggct acaaagaagt gaaaaaggac ctgatcatca
     6481 agctgcctaa gtactccctg ttcgagctgg aaaacggccg gaagagaatg ctggcctctg
     6541 ccggcgaact gcagaaggga aacgaactgg ccctgccctc caaatatgtg aacttcctgt
     6601 acctggccag ccactatgag aagctgaagg gctcccccga ggataatgag cagaaacagc
     6661 tgtttgtgga acagcacaag cactacctgg acgagatcat cgagcagatc agcgagttct
     6721 ccaagagagt gatcctggcc gacgctaatc tggacaaagt gctgtccgcc tacaacaagc
     6781 accgggataa gcccatcaga gagcaggccg agaatatcat ccacctgttt accctgacca
     6841 atctgggagc ccctgccgcc ttcaagtact ttgacaccac catcgaccgg aagaggtaca
     6901 ccagcaccaa agaggtgctg gacgccaccc tgatccacca gagcatcacc ggcctgtacg
     6961 agacacggat cgacctgtct cagctgggag gcgattcagg cggatctact aatctgtcag
     7021 atattattga aaaggagacc ggtaagcaac tggttatcca ggaatccatc ctcatgctcc
     7081 cagaggaggt ggaagaagtc attgggaaca agccggaaag cgatatactc gtgcacaccg
     7141 cctacgacga gagcaccgac gagaatgtca tgcttctgac tagcgacgcc cctgaataca
     7201 agccttgggc tctggtcata caggatagca acggtgagaa caagattaag atgctctctg
     7261 gtggttctcc caagaagaag aggaaagtcg ctagcggcag cggcgccacc aacttcagcc
     7321 tgctgaagca ggccggcgac gtggaggaga accccggccc catgaccgag tacaagccca
     7381 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     7441 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     7501 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     7561 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     7621 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     7681 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     7741 ccgtcggagt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     7801 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     7861 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     7921 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aacgcgttaa gtcgacaatc
     7981 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     8041 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     8101 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     8161 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     8221 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     8281 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     8341 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     8401 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     8461 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     8521 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga ctttaagacc
     8581 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     8641 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     8701 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     8761 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8821 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtacgtatag
     8881 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     8941 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     9001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     9061 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     9121 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     9181 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     9241 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     9301 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     9361 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     9421 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     9481 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     9541 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     9601 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     9661 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     9721 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     9781 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     9841 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     9901 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     9961 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
    10021 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
    10081 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
    10141 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
    10201 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
    10261 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
    10321 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
    10381 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
    10441 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
    10501 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
    10561 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
    10621 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
    10681 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
    10741 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
    10801 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
    10861 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
    10921 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
    10981 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    11041 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
    11101 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
    11161 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
    11221 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
    11281 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    11341 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    11401 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    11461 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
    11521 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    11581 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    11641 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
    11701 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
    11761 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
    11821 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
    11881 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
    11941 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
    12001 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
    12061 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaag
//
