LOCUS       sequence-211407-       12933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12933)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 12933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU72.2-2.2
FEATURES             Location/Qualifiers
     source          1..12933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1919..2218
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        5046..5266
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             5267..6070
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(8829..8849)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8829..8847)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(9186..9774)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9266..9285)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9945..10736,10737..10805))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10568..10587
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10806..10910)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10937..12279
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(11900..11947)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     12320..12338
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(12680..12696)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12680..12696)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 aaataagaga atttcagatt gagagaatga aaaaaaaaaa aaaaaaaaag gcagaggaga
       61 gcatagaaat ggggttcact ttttggtaaa gctatagcat gcctatcaca tataaataga
      121 gtgccagtag cgactttttt cacactcgaa atactcttac tactgctctc ttgttgtttt
      181 tatcacttct tgtttcttct tggtaaatag aatatcaagc tacaaaaagc atacaatcaa
      241 ctatcaacta ttaactatat cgtaatacac aatgcgtcgt tcttcactag cctaccgtcg
      301 tgtcctcccc ggtgcaccca cctctcgatc tgctgcctcc tacgctatcg gctttgaaaa
      361 cgctcaagag cagcgtgtcc ccgtcgacct taccatccat ggcacgattc cgtcctggct
      421 tagtggtgtc ttatatcgga caggaccagg aacctaccgg attccctcaa ccaataaccc
      481 atcaggatcc gttgacattc aacactggtt tgacggactc agcatgaatc atcgcttcga
      541 gatccatccc ggtggaagtc gagtatctta tcactctagg aaggcctctg aggactacga
      601 gaaacagatc atagaacacg gcaaagtccc cggcattgcc tttggtcaac gaccagacat
      661 ttgccagagc atctttcgca agttcttcac ggtctttcag cagatccgct ctccaccaca
      721 acccagtact tctccgtccg gactcaatgt gcaagtcacc ttgacgccag agatgcctgg
      781 atggaagagc ttgctccaac ttgatcaccg ccacacttct ggtccacagt acattgtcgc
      841 caagaccgat gcagatggcc ttcaggtcat tgatcctgtc tccctcgagc cactcgctac
      901 agggacctat aagacattgg atccacgact cgatggtcag ctcagcgccg cgcattcatg
      961 ccgcgacaag gagactaacg agttcttcaa ttactcctgc aaggttggcg gtcgttttgc
     1021 tacgtacaag gtgtttagaa tcaatggtga tgatatagtg gacgtccttg ctgagatcaa
     1081 agacgcgcct gcttcatacc ttcactcctt tgctatgacc tcgaagtacg tcgtccttgc
     1141 ggtctggcaa gctcatatca ctgggtatgg gctgtcgata ctctacaacc ggaatatcgc
     1201 agagtccatt gacaagaaat ggaatcccaa tttaaagagc gtcttctacg tcattgataa
     1261 gaggaatgga ggcattgtag cgaaatatga gactcccccg ttcttctgtt tccatcaact
     1321 caatgcctac gacgatcctg cgaaggacga tatcgtagtg gatatgtcag tctacgatga
     1381 caacacggta atagaccgcc tctacctcga taagcttcgc aatctcacag ccgagaaccc
     1441 catgctcatg ggacgtgctc gccgttttcg ccttaatgcc gtttcctctt catccaagtc
     1501 cacaagacca gccatcgtcg acttcacctt ttcgcaatct gaatcaatcg aacttcccac
     1561 tattaaccca ttcagctacc acaagcctta ccgttacgcg tatggaatca acaagctgga
     1621 tcctcgggcc catcatacgt tcgcagatag gatcatcaag ctcgacatgc agaacatcaa
     1681 tcacggacat aaggtctggg gtatcccagg ctataccccg agcgaaccga tattcgttcc
     1741 acgtcccggg tcagaagctg aggacgacgg tgtccttctt agcgtggttc tcgatggaga
     1801 gcgcggaagg agcatgttga ttgtactgaa tgcagaggat atgactgaat gtgctagggc
     1861 cgagatggag acaacttttc ccattggatt ccatggcatt tggacttcga cggagtgagc
     1921 ggatctctta tgtctttacg atttatagtt ttcattatca agtatgccta tattagtata
     1981 tagcatcttt agatgacagt gttcgaagtt tcacgaataa aagataatat tctacttttt
     2041 gctcccaccg cgtttgctag cacgagtgaa caccatccct cgcctgtgag ttgtacccat
     2101 tcctctaaac tgtagacatg gtagcttcag cagtgttcgt tatgtacggc atcctccaac
     2161 aaacagtcgg ttatagtttg tcctgctcct ctgaatcgtc tccctcgata tttctcattt
     2221 tccttcgcat gccagcattg aaatgatcga agttcaatga tgaaacggta attcttctgt
     2281 catttactca tctcatctca tcaagttata taattctata cggatgtaat ttttcacttt
     2341 tcgtcttgac gtccacccta taatttcaat tattgaaccc tcacgatcca gttctccagt
     2401 gacacagcct ttatctggtc aaacctttct ttctaatcac ctatgctgat gcttaattaa
     2461 gggatttttg tctccatcaa cggcatgcgc ccaaaaatga cgtttttttt aacccataga
     2521 cacgaaacta cccattttcc accggcctga cctaccaccg gaacaacggc catctccaac
     2581 ttgcaagttg gggaaattaa gagcatcgca ggtttaatgg aagaaaaaaa aaaggtacag
     2641 cacagcgcaa atggagttag ttcccttatg tcacacactc acacacagtc ggtcagatca
     2701 agcatactgg gtgcgtataa atagagtggc cattgccacc ctgtttatct caaaatctgt
     2761 cttgttagtg gtcttctccc tttttcaggt tacaattctc ttgtttctac ttagtatata
     2821 agtatatcaa gctatattaa gcatactatc aactgtcaac tctatcctca aaatacaata
     2881 caaaatggca tccaactgga aaccaaaacg ctcgtttgaa gaagtggaga aactgttatg
     2941 tgcaccagga acactgttgg aaatggaaca gaggttgatt gctggccaag ttcagcgcgt
     3001 ctacaaaaac ttatggccat ctgtccgact attctttctc tccgtcaccg ccctacatgc
     3061 ggacaagatc aatgtggtgt ttgagaacga acgtctgact tttcaggaga tgaatgagcg
     3121 tgccactcga gcggcagcga ttcttcgcga tgtctatggt gtattgaagg gtgaccgtgt
     3181 agccatttgc tctcgcaact ttcctgaata cctggtcgtc ttttgggcta tccatctcct
     3241 tggagctgta ccagtattag tgaacgcttg gttaccaagc gaacctctag agcattgtct
     3301 gacgcgggcg cggcccaaac tcatattctt ggatccagaa agagcaaaga tcgtgcaacc
     3361 tatggtgcca aaattgtcca aaataggcgt gaaagcatat atcgttttgc aaagtcacga
     3421 agggaaagga cagtggccag gcatgcagac ttgggaggcc gttacgaaga gctatagggg
     3481 agacgcttca accgttctga cccaagaccc cggtatcctc cccgaagata actgcattat
     3541 tatcttcacc tcagggtcga cgggacttcc aaagggcgtc cttttgagtc agcgggcgtt
     3601 tgttactgct atcggcaatg ccctcgctgg acgaggtcgt gccttactcc gtaagggaga
     3661 atcattccct ccgcccccgg cagaaggtcc tcagccggga ttgttactac caacaccgtt
     3721 cttccatgtt accggtacaa ccttgacttt atttgctgct gtcggcggag ccaaactcgt
     3781 tctaatgcgc aagtgggatg tgaaagaagc cgtcagacta ttcaaagcgg agaacatcac
     3841 agctgctggc ggtgtaccct caacagtagt tgacttgctc gacaacggag gatctggaca
     3901 tccactagat ggcgtgatgc ttggaggtgc agccgtctcg tcgacgatag ctgcgcgagc
     3961 caaggcagcg ttccctggtt ccacattatc acaggcttat ggtcagacag agtgcaatgc
     4021 gactgctgtt ggattctctg gagaggacta tgagacccgg ctcagttcat gcggacgcgc
     4081 tatgcctgtc aacgatctgt taatcatgaa agacgatgta gagtgcaagc tgggtgaacc
     4141 aggtgaagtc tggattcggg gaccgaacgt tatgaaggaa tattggggcg atccagtcgc
     4201 cacagcaaag gtcctcacga aagatggctg ggtgaaaact ggtgacatcg gatacatgga
     4261 tgaagaaggg ttcctttaca tcaaagaccg gttgaaagat atcatcattc gtggaggtga
     4321 aaatgtcgac tctgtgatgg ttgagaacgc tttatatagg gatcctcgga tctttgaagc
     4381 agctgctgtc ggggtccctg acagacgact aggcgaattg gtcgctgccc tcgtcacgtt
     4441 gaaatctgcg tatagaggca aaggacaggt tacagaagcc gagctgctgg ctttagctcg
     4501 gaaacattta cctaggttcg cagtacccgt gatgattttg attcgggaag gagattttga
     4561 gcacaccccg tctgagaaga tcatcaagat gcaactacga aagattgccg ctgaagagtg
     4621 ggagaaacgc aaggcttctg aaaacggaca ggccaaattg tagacaaatc gctcttaaat
     4681 atatacctaa agaacattaa agctatatta taagcaaaga tacgtaaatt ttgcttatat
     4741 tattatacac atatcatatt tctatatttt taagatttgg ttatataatg tacgtaatgc
     4801 aaaggaaata aattttatac attattgaac agcgtccaag taactacatt atgtgcacta
     4861 atagtttagc gtcgtgaaga ctttattgtg tcgcgaaaag taaaaatttt aaaaattaga
     4921 gcaccttgaa cttgcgaaaa aggttctcat caactgttta aaaggaggat atcaggtcct
     4981 atttctgaca aacaatatac aaatttagtt tcaaagatga atcagtgcgc gaaggacata
     5041 actcattcaa ttcaattcat catttttttt ttattctttt ttttgatttc ggtttccttg
     5101 aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg agcacagact
     5161 tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt
     5221 aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt cgaaagctac
     5281 atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat ttaatatcat
     5341 gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca aggaattact
     5401 ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg tggatatctt
     5461 gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa
     5521 ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta
     5581 ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt
     5641 gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa aggaacctag
     5701 aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg gagaatatac
     5761 taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca
     5821 aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg
     5881 tttagatgac aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc
     5941 tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa
     6001 ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca
     6061 gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaattagagc
     6121 ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac agatgcgtaa
     6181 ggagaaaata ccgcatcagg ataggaaaaa accgagcttc ctttcatccg gcgcggctgt
     6241 gttctacata tcactgaagc tccgggtatt ttaagttata caagggaaag atgccggcta
     6301 gactagcaag ttttaggctg cttaacatta tggataggcg gataaagggc ccaaacagga
     6361 ttgtaaagct tagacgcttc tggttggaca atggtacgtt tgtgtattaa gtaaggcttg
     6421 gctggggata gcaacattgg gcagagtata gaagaccaca aaaaaaaggt atataagggc
     6481 agagaagtct ttgtaatgtg tgtaacttct cttccatgtg taatcagtat ttctacttac
     6541 ttcttaaata tacagaagta agacagataa ccaacagcct ttcccagata tacatatata
     6601 tctttatttc agcttaaaca ataattatat ttgtttaact caaaaataaa aaaaaaaaaa
     6661 ccaaactcac gcaactaatt attccataat aaaataacaa catgtcgacc agggaaaaac
     6721 aaaacagcgc ggacacagtt ctcgagaatg acgaggctga attggcgagg atgggttaca
     6781 agcaggagtt gaagagggac ttggggttat tacagaattt tggaatgtcc ttctcaatca
     6841 tatcggtcat gactggaata ccatcccttt tcgcatatgg actcaatacc ggaggaccgg
     6901 cagtgatggt atggggatgg atcatagttt ccgtgtttac aatgcttgtg ggcctggcca
     6961 tggcagaggt ttgcagtgcc cacccgactt ccggaggacc ctacttttgg gctgctatgt
     7021 tatcgagtcc tgaagacgca ccactgctat catggataac ggggtggttc aacttgttgg
     7081 gacaagtcgc tgtcaccact ggaatcagct ttggttgcgc tagcttcata tcaacaatct
     7141 gcacattcaa cacagatttc gagcctacac caaacactca aattggtata tacgccgccg
     7201 tactcgtcag ccaaggcctc ataaatacct ttggagtcca cctcctacat cacctcaaca
     7261 acatctcagt ctggtggcat gctgctggga cattctcagt agtagtcgcc atactagcca
     7321 aagcacccac ccatcaatcg gcgcatttcg tctttcagac attcctggat ggtaccggtg
     7381 gatgggcaga aagagctagc ccagcttatg tagccgtcat cgggattctt atggcacagt
     7441 atacgcttac cggattcgat gctagcgctc atatgaccga agagacacgc aatgccgcca
     7501 tgtcgggaag tcttggcatt attatggcca ttggcgtttc tgcgctgcta ggatggtatc
     7561 tcatcctggg attgctgttt tcgattcaaa atttggaccg aacactcgat tctccaagtg
     7621 gacaaccggt cgctcagata ttcatcgata ccgttggcgt gaatggcgca attgcactta
     7681 tggtagtggt tattggatgc atgtactttt gcgggacatt ctcaataacg tcaaactctc
     7741 gcatgatgta cgcctttgcg agagatggtg gcattcctgg ctcccaattc tttgcctatg
     7801 ttaacacgaa atggaagtcg ccgatacgaa cggtctggct tgcctgcact ctgagtttca
     7861 tcctcgggtt gcccagctta ggtagcactg ttgcattctc agcggcaaca tcgatcgcca
     7921 ctattggatt gtacacctca tacggcatac ctatcgcatt acgaatcgtc taccaccaga
     7981 aatttgtcaa aggacccttc catctacaca agttctcctt ccccgtcgct atcacagctg
     8041 ttctgtggat cgcgtttatc tcgatcgtct ttattctacc acaagtcaac cctgtcgatt
     8101 cgcagacgct caactattcg attgtcgctg ttggcatcgt catcatatat agcgtagggt
     8161 tttggttgtt gagtgctaga aagtggttta ctggacctgt caagcagatt gctgctgagg
     8221 agatgggtat taatgttatg gagccaggaa cagcagagaa agttgaagag atggagaggg
     8281 agaaggctcg taggagattg ataagacttt tctagttgca tatcttttat atttaaatct
     8341 tatctattag ttaatttttt gtaatttatc cttatatata gtctggttat tctaaaatat
     8401 catttcagta tctaaaaatt cccctctttt ttcagttata tcttaacagg cgacagtcca
     8461 aatgttgatt tatcccagtc cgattcatca gggttgtgaa gcattttgtc aatggtcgaa
     8521 atcacatcag taatagtgcc tcttacttgc ctcatagaat ttctttctct taacgtcacc
     8581 gtttggtctt ttatagtttc gaaatctatg gtgataccaa atggtgttcc caattcatcg
     8641 ttacgggcgt attttttacc aattgaagta ttggaatcgt caattttaaa gtatatctct
     8701 cttttacgta aagcctgcga gatcctctta agtatagcgg ggaagccatc gttattcgat
     8761 attgtcgtaa caaatacttt gatcggcgct atgcggccgc caccgcggtg gagctccagc
     8821 ttttgttccc tttagtgagg gttaattgcg cgcttggcga gactcaaaca agatatattt
     8881 tgcccgcctt acagacgaaa ctagttggag gttatggagc atactatcac gtgggcggcc
     8941 actggtgagt tactacaccc caggggcaac gttgatgctc ctaaaaaact ctggctggac
     9001 gcaagccgta acacccgtgt cacttcataa tcgtttgcaa ttcagggctt gatctacaca
     9061 ttaccctgtt atccctatta attaatacgg ttatccacag aatcagggga taacgcagga
     9121 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     9181 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     9241 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     9301 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     9361 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     9421 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     9481 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     9541 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     9601 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     9661 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9721 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     9781 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     9841 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     9901 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     9961 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    10021 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    10081 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    10141 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    10201 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    10261 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    10321 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    10381 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    10441 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    10501 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    10561 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    10621 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    10681 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    10741 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    10801 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    10861 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    10921 cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc
    10981 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
    11041 cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc
    11101 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
    11161 tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa
    11221 aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt
    11281 tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag
    11341 aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg
    11401 cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat
    11461 tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg
    11521 attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt
    11581 ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac
    11641 aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt
    11701 agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag
    11761 atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta
    11821 gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt
    11881 tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag
    11941 gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat
    12001 acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga
    12061 gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta
    12121 ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat
    12181 gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt
    12241 ctcatccttc aatgctatca tttcctttga tattggatca tactaagaaa ccattattat
    12301 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
    12361 tgatgacggt gagcgcgttt cggtgatgac ggtgaaaacc tcggcgcgcc attaccctgt
    12421 tatccctaat atacaacgca aagaagctgg aaaattggcg agggaatcct gtttctgtct
    12481 atccaagaat gggcatgagg tggcaaccgt cgtgctagcg tacagggtgc actttgtaac
    12541 catttgggac accggacact cgctgttttc gaaattaccc tttaagcgcg ggtattgaac
    12601 caggcttatg cccaagatcg tagcaagcat ttaaatgacg gtatcgataa gcttgatatc
    12661 gaattcctgc agcccggggg atccactagt tctagattaa ttaatatcta aaaattgcct
    12721 tatgatccgt ctctccggtt acagcctgtg taactgatta atcctgcctt tctaatcacc
    12781 attctaatgt tttaattaag ggattttgtc ttcattaacg gctttcgctc ataaaaatgt
    12841 tatgacgttt tgcccgcagg cgggaaacca tccacttcac gagactgatc tcctctgccg
    12901 gaacaccggg catctccaac ttataagttg gag
//
