LOCUS       sequence-211408-       12418 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of BE3 (not codon 
            optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12418)
  AUTHORS   Zafra MP, Schatoff EM, Katti A, Foronda M, Breinig M, Schweitzer AY,
            Simon A, Han T, Goswami S, Montgomery E, Thibado J, Kastenhuber ER, 
            Sanchez-Rivera FJ, Shi J, Vakoc CR, Lowe SW, Tschaharganeh DF, Dow 
            LE
  TITLE     Optimized base editors enable efficient editing in cells, organoids 
            and mice.
  JOURNAL   Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
  PUBMED    29969439
REFERENCE   2  (bases 1 to 12418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194."
COMMENT     Sequence Label: pLenti-BE3-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..12418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(38..57)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        229..608
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        610..808
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     763..783
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             826..1006
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1053..1178
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1675..1908
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2093..2137
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2435..2552
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2670..2881
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2932..3618
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             join(3667..7065,7066..7068,7069..7665,7666,7667..7669,
                     7670..7671,7672..7674,7675..7767)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(3667..3690,3691..3693,3694..7767)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7780..8028
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             8041..8061
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8070..8087
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        8126..8625
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8161..8184)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8558..8577
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8646..9245
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8646..8665)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9142..9162
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9298..9886
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9351..9371)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9769..9780)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9889..9905)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9890..9906)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10415..10595
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(10657..11245)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10737..10756)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11416..12207,12208..12276))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12039..12058
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12277..12381)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
       61 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
      121 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
      181 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
      241 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
      301 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      361 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      421 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      481 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      541 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      601 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      661 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      721 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      781 gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta
      841 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
      901 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
      961 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     1021 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     1081 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     1141 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     1201 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     1261 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     1321 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     1381 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     1441 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     1501 ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1861 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1981 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2281 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
     2401 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     2461 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     2521 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     2581 cagtttggtt agtaccgggc ccgggtcgac tagtgatcag tgtgagggag tgtaaagctg
     2641 gttttcgagt ggctccagtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     2701 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa
     2761 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     2821 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     2881 ggtgtcgtga cgcggctaac tcgaacgcta gctgtgcgat cgtttgccac catgagctca
     2941 gagactggcc cagtggctgt ggaccccaca ttgagacggc ggatcgagcc ccatgagttt
     3001 gaggtattct tcgatccgag agagctccgc aaggagacct gcctgcttta cgaaattaat
     3061 tgggggggcc ggcactccat ttggcgacat acatcacaga acactaacaa gcacgtcgaa
     3121 gtcaacttca tcgagaagtt cacgacagaa agatatttct gtccgaacac aaggtgcagc
     3181 attacctggt ttctcagctg gagcccatgc ggcgaatgta gtagggccat cactgaattc
     3241 ctgtcaaggt atccccacgt cactctgttt atttacatcg caaggctgta ccaccacgct
     3301 gacccccgca atcgacaagg cctgcgggat ttgatctctt caggtgtgac tatccaaatt
     3361 atgactgagc aggagtcagg atactgctgg agaaactttg tgaattatag cccgagtaat
     3421 gaagcccact ggcctaggta tccccatctg tgggtacgac tgtacgttct tgaactgtac
     3481 tgcatcatac tgggcctgcc tccttgtctc aacattctga gaaggaagca gccacagctg
     3541 acattcttta ccatcgctct tcagtcttgt cattaccagc gactgccccc acacattctc
     3601 tgggccaccg ggttgaaaag cggcagcgag actcccggga cctcagagtc cgccacaccc
     3661 gaaagtgata aaaagtattc tattggttta gccatcggca ctaattccgt tggatgggct
     3721 gtcataaccg atgaatacaa agtaccttca aagaaattta aggtgttggg gaacacagac
     3781 cgtcattcga ttaaaaagaa tcttatcggt gccctcctat tcgatagtgg cgaaacggca
     3841 gaggcgactc gcctgaaacg aaccgctcgg agaaggtata cacgtcgcaa gaaccgaata
     3901 tgttacttac aagaaatttt tagcaatgag atggccaaag ttgacgattc tttctttcac
     3961 cgtttggaag agtccttcct tgtcgaagag gacaagaaac atgaacggca ccccatcttt
     4021 ggaaacatag tagatgaggt ggcatatcat gaaaagtacc caacgattta tcacctcaga
     4081 aaaaagctag ttgactcaac tgataaagcg gacctgaggt taatctactt ggctcttgcc
     4141 catatgataa agttccgtgg gcactttctc attgagggtg atctaaatcc ggacaactcg
     4201 gatgtcgaca aactgttcat ccagttagta caaacctata atcagttgtt tgaagagaac
     4261 cctataaatg caagtggcgt ggatgcgaag gctattctta gcgcccgcct ctctaaatcc
     4321 cgacggctag aaaacctgat cgcacaatta cccggagaga agaaaaatgg gttgttcggt
     4381 aaccttatag cgctctcact aggcctgaca ccaaatttta agtcgaactt cgacttagct
     4441 gaagatgcca aattgcagct tagtaaggac acgtacgatg acgatctcga caatctactg
     4501 gcacaaattg gagatcagta tgcggactta tttttggctg ccaaaaacct tagcgatgca
     4561 atcctcctat ctgacatact gagagttaat actgagatta ccaaggcgcc gttatccgct
     4621 tcaatgatca aaaggtacga tgaacatcac caagacttga cacttctcaa ggccctagtc
     4681 cgtcagcaac tgcctgagaa atataaggaa atattctttg atcagtcgaa aaacgggtac
     4741 gcaggttata ttgacggcgg agcgagtcaa gaggaattct acaagtttat caaacccata
     4801 ttagagaaga tggatgggac ggaagagttg cttgtaaaac tcaatcgcga agatctactg
     4861 cgaaagcagc ggactttcga caacggtagc attccacatc aaatccactt aggcgaattg
     4921 catgctatac ttagaaggca ggaggatttt tatccgttcc tcaaagacaa tcgtgaaaag
     4981 attgagaaaa tcctaacctt tcgcatacct tactatgtgg gacccctggc ccgagggaac
     5041 tctcggttcg catggatgac aagaaagtcc gaagaaacga ttactccatg gaattttgag
     5101 gaagttgtcg ataaaggtgc gtcagctcaa tcgttcatcg agaggatgac caactttgac
     5161 aagaatttac cgaacgaaaa agtattgcct aagcacagtt tactttacga gtatttcaca
     5221 gtgtacaatg aactcacgaa agttaagtat gtcactgagg gcatgcgtaa acccgccttt
     5281 ctaagcggag aacagaagaa agcaatagta gatctgttat tcaagaccaa ccgcaaagtg
     5341 acagttaagc aattgaaaga ggactacttt aagaaaattg aatgcttcga ttctgtcgag
     5401 atctccgggg tagaagatcg atttaatgcg tcacttggta cgtatcatga cctcctaaag
     5461 ataattaaag ataaggactt cctggataac gaagagaatg aagatatctt agaagatata
     5521 gtgttgactc ttaccctctt tgaagatcgg gaaatgattg aggaaagact aaaaacatac
     5581 gctcacctgt tcgacgataa ggttatgaaa cagttaaaga ggcgtcgcta tacgggctgg
     5641 ggacgattgt cgcggaaact tatcaacggg ataagagaca agcaaagtgg taaaactatt
     5701 ctcgattttc taaagagcga cggcttcgcc aataggaact ttatgcagct gatccatgat
     5761 gactctttaa ccttcaaaga ggatatacaa aaggcacagg tttccggaca aggggactca
     5821 ttgcacgaac atattgcgaa tcttgctggt tcgccagcca tcaaaaaggg catactccag
     5881 acagtcaaag tagtggatga gctagttaag gtcatgggac gtcacaaacc ggaaaacatt
     5941 gtaatcgaga tggcacgcga aaatcaaacg actcagaagg ggcaaaaaaa cagtcgagag
     6001 cggatgaaga gaatagaaga gggtattaaa gaactgggca gccagatctt aaaggagcat
     6061 cctgtggaaa atacccaatt gcagaacgag aaactttacc tctattacct acaaaatgga
     6121 agggacatgt atgttgatca ggaactggac ataaaccgtt tatctgatta cgacgtcgat
     6181 cacattgtac cccaatcctt tttgaaggac gattcaatcg acaataaagt gcttacacgc
     6241 tcggataaga accgagggaa aagtgacaat gttccaagcg aggaagtcgt aaagaaaatg
     6301 aagaactatt ggcggcagct cctaaatgcg aaactgataa cgcaaagaaa gttcgataac
     6361 ttaactaaag ctgagagggg tggcttgtct gaacttgaca aggccggatt tattaaacgt
     6421 cagctcgtgg aaacccgcca aatcacaaag catgttgcac agatactaga ttcccgaatg
     6481 aatacgaaat acgacgagaa cgataagctg attcgggaag tcaaagtaat cactttaaag
     6541 tcaaaattgg tgtcggactt cagaaaggat tttcaattct ataaagttag ggagataaat
     6601 aactaccacc atgcgcacga cgcttatctt aatgccgtcg tagggaccgc actcattaag
     6661 aaatacccga agctagaaag tgagtttgtg tatggtgatt acaaagttta tgacgtccgt
     6721 aagatgatcg cgaaaagcga acaggagata ggcaaggcta cagccaaata cttcttttat
     6781 tctaacatta tgaatttctt taagacggaa atcactctgg caaacggaga gatacgcaaa
     6841 cgacctttaa ttgaaaccaa tggggagaca ggtgaaatcg tatgggataa gggccgggac
     6901 ttcgcgacgg tgagaaaagt tttgtccatg ccccaagtca acatagtaaa gaaaactgag
     6961 gtgcagaccg gagggttttc aaaggaatcg attcttccaa aaaggaatag tgataagctc
     7021 atcgctcgta aaaaggactg ggacccgaaa aagtacggtg gcttcgatag ccctacagtt
     7081 gcctattctg tcctagtagt ggcaaaagtt gagaagggaa aatccaagaa actgaagtca
     7141 gtcaaagaat tattggggat aacgattatg gagcgctcgt cttttgaaaa gaaccccatc
     7201 gacttccttg aggcgaaagg ttacaaggaa gtaaaaaagg atctcataat taaactacca
     7261 aagtatagtc tgtttgagtt agaaaatggc cgaaaacgga tgttggctag cgccggagag
     7321 cttcaaaagg ggaacgaact cgcactaccg tctaaatacg tgaatttcct gtatttagcg
     7381 tcccattacg agaagttgaa aggttcacct gaagataacg aacagaagca actttttgtt
     7441 gagcagcaca aacattatct cgacgaaatc atagagcaaa tttcggaatt cagtaagaga
     7501 gtcatcctag ctgatgccaa tctggacaaa gtattaagcg catacaacaa gcacagggat
     7561 aaacccatac gtgagcaggc ggaaaatatt atccatttgt ttactcttac caacctcggc
     7621 gctccagccg cattcaagta ttttgacaca acgatagatc gcaaacgata cacttctacc
     7681 aaggaggtgc tagacgcgac actgattcac caatccatca cgggattata tgaaactcgg
     7741 atagatttgt cacagcttgg gggtgactct ggtggttcta ctaatctgtc agatattatt
     7801 gaaaaggaga ccggtaagca actggttatc caggaatcca tcctcatgct cccagaggag
     7861 gtggaagaag tcattgggaa caagccggaa agcgatatac tcgtgcacac cgcctacgac
     7921 gagagcaccg acgagaatgt catgcttctg actagcgacg cccctgaata caagccttgg
     7981 gctctggtca tacaggatag caacggtgag aacaagatta agatgctctc tggtggttct
     8041 cccaagaaga agaggaaagt ctaaccggtc atcatcacca tcaccattga aaaccgctgt
     8101 tcctaggaat cccgaggcct ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
     8161 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
     8221 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
     8281 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
     8341 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
     8401 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
     8461 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg
     8521 ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc
     8581 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc
     8641 ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     8701 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     8761 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     8821 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     8881 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     8941 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     9001 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     9061 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     9121 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     9181 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     9241 cctgaaaacg ttgttgtttg gggttgaatt actcttctag accgcggcgc gcccgataat
     9301 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     9361 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     9421 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     9481 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     9541 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     9601 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     9661 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     9721 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     9781 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     9841 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
     9901 cctcgatcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     9961 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
    10021 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
    10081 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
    10141 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
    10201 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
    10261 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
    10321 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
    10381 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
    10441 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
    10501 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
    10561 cagacccttt tagtcagtgt ggaaaatctc tagcagcatg tgagcaaaag gccagcaaaa
    10621 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    10681 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    10741 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    10801 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    10861 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    10921 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    10981 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    11041 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    11101 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    11161 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    11221 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    11281 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    11341 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    11401 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    11461 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    11521 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    11581 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    11641 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    11701 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    11761 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    11821 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    11881 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    11941 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    12001 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    12061 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    12121 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    12181 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    12241 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    12301 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    12361 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacgga
//
