LOCUS       sequence-211414-       11188 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of Cas9-VRER (not 
            codon optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11188)
  AUTHORS   Zafra MP, Schatoff EM, Katti A, Foronda M, Breinig M, Schweitzer AY,
            Simon A, Han T, Goswami S, Montgomery E, Thibado J, Kastenhuber ER, 
            Sanchez-Rivera FJ, Shi J, Vakoc CR, Lowe SW, Tschaharganeh DF, Dow 
            LE
  TITLE     Optimized base editors enable efficient editing in cells, organoids 
            and mice.
  JOURNAL   Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
  PUBMED    29969439
REFERENCE   2  (bases 1 to 11188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194."
COMMENT     Sequence Label: pLenti-VRER-P2A-Puro
FEATURES             Location/Qualifiers
     source          1..11188
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..235
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             236..416
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    463..588
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1081..1314
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1499..1543
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1727..1844
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1913..2124
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2144..2153
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2150..5551,5552..5554,5555..5800,5801..5803,5804..6151,
                     6152..6154,6155..6157,6158..6160,6161..6253)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/G1218R/R1335E/T1337R mutations"
                     /label=Cas9 VRER
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKEYRSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6260..6280
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6287..6352
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6377..6433
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6434..7033
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6434..6453)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6930..6950
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7049..7637
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7102..7122)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7520..7531)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7709..7942
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8020..8141
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8057..8076)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8111..8130
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8181..8316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8336..8355)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8337..8355)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8365..8382)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8365..8381)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8374..8396)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8523..8978
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8610..8629)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8820..8841
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9004..9108
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9109..9177,9178..9969)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9327..9346)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10140..10728
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10629..10648
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10882..10899
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11016..11037
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11052..11057,11058..11075,11076..11082)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11090..11106
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11095..11117
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11114..11130
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11114..11130
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11149..11169
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11151..11169
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     1681 atcgtttcag acccacctcc caaccccgag gggacccaga ccacaatttt aaaagaaaag
     1741 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     1801 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     1861 acagcagaga tccactttgg cgccggctcg agtggctccg gtgcccgtca gtgggcagag
     1921 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
     1981 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     2041 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     2101 aacgggtttg ccgccagaac acaggtgtcg tgacgcggga tccgccacca tggataaaaa
     2161 gtattctatt ggtttagaca tcggcactaa ttccgttgga tgggctgtca taaccgatga
     2221 atacaaagta ccttcaaaga aatttaaggt gttggggaac acagaccgtc attcgattaa
     2281 aaagaatctt atcggtgccc tcctattcga tagtggcgaa acggcagagg cgactcgcct
     2341 gaaacgaacc gctcggagaa ggtatacacg tcgcaagaac cgaatatgtt acttacaaga
     2401 aatttttagc aatgagatgg ccaaagttga cgattctttc tttcaccgtt tggaagagtc
     2461 cttccttgtc gaagaggaca agaaacatga acggcacccc atctttggaa acatagtaga
     2521 tgaggtggca tatcatgaaa agtacccaac gatttatcac ctcagaaaaa agctagttga
     2581 ctcaactgat aaagcggacc tgaggttaat ctacttggct cttgcccata tgataaagtt
     2641 ccgtgggcac tttctcattg agggtgatct aaatccggac aactcggatg tcgacaaact
     2701 gttcatccag ttagtacaaa cctataatca gttgtttgaa gagaacccta taaatgcaag
     2761 tggcgtggat gcgaaggcta ttcttagcgc ccgcctctct aaatcccgac ggctagaaaa
     2821 cctgatcgca caattacccg gagagaagaa aaatgggttg ttcggtaacc ttatagcgct
     2881 ctcactaggc ctgacaccaa attttaagtc gaacttcgac ttagctgaag atgccaaatt
     2941 gcagcttagt aaggacacgt acgatgacga tctcgacaat ctactggcac aaattggaga
     3001 tcagtatgcg gacttatttt tggctgccaa aaaccttagc gatgcaatcc tcctatctga
     3061 catactgaga gttaatactg agattaccaa ggcgccgtta tccgcttcaa tgatcaaaag
     3121 gtacgatgaa catcaccaag acttgacact tctcaaggcc ctagtccgtc agcaactgcc
     3181 tgagaaatat aaggaaatat tctttgatca gtcgaaaaac gggtacgcag gttatattga
     3241 cggcggagcg agtcaagagg aattctacaa gtttatcaaa cccatattag agaagatgga
     3301 tgggacggaa gagttgcttg taaaactcaa tcgcgaagat ctactgcgaa agcagcggac
     3361 tttcgacaac ggtagcattc cacatcaaat ccacttaggc gaattgcatg ctatacttag
     3421 aaggcaggag gatttttatc cgttcctcaa agacaatcgt gaaaagattg agaaaatcct
     3481 aacctttcgc ataccttact atgtgggacc cctggcccga gggaactctc ggttcgcatg
     3541 gatgacaaga aagtccgaag aaacgattac tccatggaat tttgaggaag ttgtcgataa
     3601 aggtgcgtca gctcaatcgt tcatcgagag gatgaccaac tttgacaaga atttaccgaa
     3661 cgaaaaagta ttgcctaagc acagtttact ttacgagtat ttcacagtgt acaatgaact
     3721 cacgaaagtt aagtatgtca ctgagggcat gcgtaaaccc gcctttctaa gcggagaaca
     3781 gaagaaagca atagtagatc tgttattcaa gaccaaccgc aaagtgacag ttaagcaatt
     3841 gaaagaggac tactttaaga aaattgaatg cttcgattct gtcgagatct ccggggtaga
     3901 agatcgattt aatgcgtcac ttggtacgta tcatgacctc ctaaagataa ttaaagataa
     3961 ggacttcctg gataacgaag agaatgaaga tatcttagaa gatatagtgt tgactcttac
     4021 cctctttgaa gatcgggaaa tgattgagga aagactaaaa acatacgctc acctgttcga
     4081 cgataaggtt atgaaacagt taaagaggcg tcgctatacg ggctggggac gattgtcgcg
     4141 gaaacttatc aacgggataa gagacaagca aagtggtaaa actattctcg attttctaaa
     4201 gagcgacggc ttcgccaata ggaactttat gcagctgatc catgatgact ctttaacctt
     4261 caaagaggat atacaaaagg cacaggtttc cggacaaggg gactcattgc acgaacatat
     4321 tgcgaatctt gctggttcgc cagccatcaa aaagggcata ctccagacag tcaaagtagt
     4381 ggatgagcta gttaaggtca tgggacgtca caaaccggaa aacattgtaa tcgagatggc
     4441 acgcgaaaat caaacgactc agaaggggca aaaaaacagt cgagagcgga tgaagagaat
     4501 agaagagggt attaaagaac tgggcagcca gatcttaaag gagcatcctg tggaaaatac
     4561 ccaattgcag aacgagaaac tttacctcta ttacctacaa aatggaaggg acatgtatgt
     4621 tgatcaggaa ctggacataa accgtttatc tgattacgac gtcgatcaca ttgtacccca
     4681 atcctttttg aaggacgatt caatcgacaa taaagtgctt acacgctcgg ataagaaccg
     4741 agggaaaagt gacaatgttc caagcgagga agtcgtaaag aaaatgaaga actattggcg
     4801 gcagctccta aatgcgaaac tgataacgca aagaaagttc gataacttaa ctaaagctga
     4861 gaggggtggc ttgtctgaac ttgacaaggc cggatttatt aaacgtcagc tcgtggaaac
     4921 ccgccaaatc acaaagcatg ttgcacagat actagattcc cgaatgaata cgaaatacga
     4981 cgagaacgat aagctgattc gggaagtcaa agtaatcact ttaaagtcaa aattggtgtc
     5041 ggacttcaga aaggattttc aattctataa agttagggag ataaataact accaccatgc
     5101 gcacgacgct tatcttaatg ccgtcgtagg gaccgcactc attaagaaat acccgaagct
     5161 agaaagtgag tttgtgtatg gtgattacaa agtttatgac gtccgtaaga tgatcgcgaa
     5221 aagcgaacag gagataggca aggctacagc caaatacttc ttttattcta acattatgaa
     5281 tttctttaag acggaaatca ctctggcaaa cggagagata cgcaaacgac ctttaattga
     5341 aaccaatggg gagacaggtg aaatcgtatg ggataagggc cgggacttcg cgacggtgag
     5401 aaaagttttg tccatgcccc aagtcaacat agtaaagaaa actgaggtgc agaccggagg
     5461 gttttcaaag gaatcgattc ttccaaaaag gaatagtgat aagctcatcg ctcgtaaaaa
     5521 ggactgggac ccgaaaaagt acggtggctt cgtgagccct acagttgcct attctgtcct
     5581 agtagtggca aaagttgaga agggaaaatc caagaaactg aagtcagtca aagaattatt
     5641 ggggataacg attatggagc gctcgtcttt tgaaaagaac cccatcgact tccttgaggc
     5701 gaaaggttac aaggaagtaa aaaaggatct cataattaaa ctaccaaagt atagtctgtt
     5761 tgagttagaa aatggccgaa aacggatgtt ggctagcgcc agagagcttc aaaaggggaa
     5821 cgaactcgca ctaccgtcta aatacgtgaa tttcctgtat ttagcgtccc attacgagaa
     5881 gttgaaaggt tcacctgaag ataacgaaca gaagcaactt tttgttgagc agcacaaaca
     5941 ttatctcgac gaaatcatag agcaaatttc ggaattcagt aagagagtca tcctagctga
     6001 tgccaatctg gacaaagtat taagcgcata caacaagcac agggataaac ccatacgtga
     6061 gcaggcggaa aatattatcc atttgtttac tcttaccaac ctcggcgctc cagccgcatt
     6121 caagtatttt gacacaacga tagatcgcaa agagtacaga tctaccaagg aggtgctaga
     6181 cgcgacactg attcaccaat ccatcacggg attatatgaa actcggatag atttgtcaca
     6241 gcttgggggt gacggatccc ccaagaagaa gaggaaagtc tcgagcgact acaaagacca
     6301 tgacggtgat tataaagatc atgacatcga ttacaaggat gacgatgaca aggctgcagg
     6361 agctagcggc agcggcgcca ccaacttcag cctgctgaag caggccggcg acgtggagga
     6421 gaaccccggc cccatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     6481 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     6541 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     6601 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     6661 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     6721 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     6781 ccggcccaag gagcccgcgt ggttcctggc caccgtcgga gtctcgcccg accaccaggg
     6841 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     6901 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     6961 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     7021 gcccggtgcc tgaacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     7081 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     7141 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     7201 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     7261 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     7321 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     7381 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     7441 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     7501 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     7561 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     7621 ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt
     7681 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     7741 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7801 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7861 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7921 tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat
     7981 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     8041 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     8101 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     8161 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     8221 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     8281 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     8341 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     8401 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     8461 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     8521 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     8581 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     8641 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     8701 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     8761 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     8821 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     8881 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     8941 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     9001 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     9061 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     9121 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     9181 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     9241 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     9301 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     9361 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     9421 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     9481 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     9541 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     9601 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     9661 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     9721 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     9781 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     9841 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     9901 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     9961 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
    10021 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    10081 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    10141 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    10201 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    10261 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
    10321 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    10381 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    10441 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    10501 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    10561 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    10621 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    10681 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    10741 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    10801 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    10861 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
    10921 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
    10981 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
    11041 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
    11101 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
    11161 cactaaaggg aacaaaagct ggagctgc
//
