LOCUS       sequence-211427-       11203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of Cas9-HF1RA in 
            mammalian cells (codon optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11203)
  AUTHORS   Zafra MP, Schatoff EM, Katti A, Foronda M, Breinig M, Schweitzer AY,
            Simon A, Han T, Goswami S, Montgomery E, Thibado J, Kastenhuber ER, 
            Sanchez-Rivera FJ, Shi J, Vakoc CR, Lowe SW, Tschaharganeh DF, Dow 
            LE
  TITLE     Optimized base editors enable efficient editing in cells, organoids 
            and mice.
  JOURNAL   Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
  PUBMED    29969439
REFERENCE   2  (bases 1 to 11203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194."
COMMENT     Sequence Label: pLenti-HF1RA-P2A-Puro
FEATURES             Location/Qualifiers
     source          1..11203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..247
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             248..428
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    475..600
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1093..1326
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1511..1555
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1739..1856
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1925..2136
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2156..2165
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2165..2188
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2195..2215
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2240..6340
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGALSRKLINGIRDKQSGKTILDFLKSDGFANRNFMA
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRAITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6341..6388
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6404..6460
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6461..7060
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6461..6480)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6957..6977
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7076..7664
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7129..7149)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7547..7558)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7736..7969
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8047..8168
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8084..8103)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8138..8157
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8208..8343
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8363..8382)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8364..8382)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8392..8409)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8392..8408)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8401..8423)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8550..9005
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8637..8656)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8847..8868
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9031..9135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9136..9204,9205..9996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9354..9373)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10167..10755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10656..10675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10909..10926
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11043..11064
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11079..11084,11085..11102,11103..11109)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11117..11133
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11122..11144
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11141..11157
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11141..11157
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11176..11196
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11178..11196
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
       61 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
      121 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
      181 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
      241 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
      301 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
      361 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
      421 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
      481 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
      541 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
      601 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
      661 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
      721 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
      781 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
      841 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
      901 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
      961 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1021 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1081 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1141 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     1201 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1261 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1321 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1381 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     1441 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     1501 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     1561 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     1621 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     1681 atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccca gaccacaatt
     1741 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     1801 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     1861 tttattacag ggacagcaga gatccacttt ggcgccggct cgagtggctc cggtgcccgt
     1921 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     1981 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     2041 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     2101 gttctttttc gcaacgggtt tgccgccaga acacaggtgt cgtgacgcgg gatccgccac
     2161 catggattac aaagacgatg acgataagat ggccccaaag aagaagcgga aggtcggtat
     2221 ccacggagtc ccagcagccg acaagaagta cagcatcggc ctggacatcg gcaccaactc
     2281 tgtgggctgg gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct
     2341 gggcaacacc gaccggcaca gcatcaagaa gaacctgatc ggagccctgc tgttcgacag
     2401 cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg
     2461 gaagaaccgg atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga
     2521 cagcttcttc cacagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg
     2581 gcaccccatc ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat
     2641 ctaccacctg agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta
     2701 tctggccctg gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa
     2761 ccccgacaac agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct
     2821 gttcgaggaa aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgtctgccag
     2881 actgagcaag agcagacggc tggaaaatct gatcgcccag ctgcccggcg agaagaagaa
     2941 tggcctgttc ggaaacctga ttgccctgag cctgggcctg acccccaact tcaagagcaa
     3001 cttcgacctg gccgaggatg ccaaactgca gctgagcaag gacacctacg acgacgacct
     3061 ggacaacctg ctggcccaga tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa
     3121 cctgtccgac gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc
     3181 ccccctgagc gcctctatga tcaagagata cgacgagcac caccaggacc tgaccctgct
     3241 gaaagctctc gtgcggcagc agctgcctga gaagtacaaa gagattttct tcgaccagag
     3301 caagaacggc tacgccggct acattgacgg cggagccagc caggaagagt tctacaagtt
     3361 catcaagccc atcctggaaa agatggacgg caccgaggaa ctgctcgtga agctgaacag
     3421 agaggacctg ctgcggaagc agcggacctt cgacaacggc agcatccccc accagatcca
     3481 cctgggagag ctgcacgcca ttctgcggcg gcaggaagat ttttacccat tcctgaagga
     3541 caaccgggaa aagatcgaga agatcctgac cttccgcatc ccctactacg tgggccctct
     3601 ggccagggga aacagcagat tcgcctggat gaccagaaag agcgaggaaa ccatcacccc
     3661 ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc cagagcttca tcgagcggat
     3721 gaccaacttc gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta
     3781 cgagtacttc accgtgtata acgagctgac caaagtgaaa tacgtgaccg agggaatgag
     3841 aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc tgttcaagac
     3901 caaccggaaa gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt
     3961 cgactccgtg gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca
     4021 cgatctgctg aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat
     4081 tctggaagat atcgtgctga ccctgacact gtttgaggac agagagatga tcgaggaacg
     4141 gctgaaaacc tatgcccacc tgttcgacga caaagtgatg aagcagctga agcggcggag
     4201 atacaccggc tggggcgccc tgagccggaa gctgatcaac ggcatccggg acaagcagtc
     4261 cggcaagaca atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatggc
     4321 cctgatccac gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg
     4381 ccagggcgat agcctgcacg agcacattgc caatctggcc ggcagccccg ccattaagaa
     4441 gggcatcctg cagacagtga aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa
     4501 gcccgagaac atcgtgatcg aaatggccag agagaaccag accacccaga agggacagaa
     4561 gaacagccgc gagagaatga agcggatcga agagggcatc aaagagctgg gcagccagat
     4621 cctgaaagaa caccccgtgg aaaacaccca gctgcagaac gagaagctgt acctgtacta
     4681 cctgcagaat gggcgggata tgtacgtgga ccaggaactg gacatcaacc ggctgtccga
     4741 ctacgatgtg gaccatatcg tgcctcagag ctttctgaag gacgactcca tcgacaacaa
     4801 ggtgctgacc agaagcgaca agaaccgggg caagagcgac aacgtgccct ccgaagaggt
     4861 cgtgaagaag atgaagaact actggcggca gctgctgaac gccaagctga ttacccagag
     4921 aaagttcgac aatctgacca aggccgagag aggcggcctg agcgaactgg ataaggccgg
     4981 cttcatcaag agacagctgg tggaaacccg ggccatcaca aagcacgtgg cacagatcct
     5041 ggactcccgg atgaacacta agtacgacga gaatgacaag ctgatccggg aagtgaaagt
     5101 gatcaccctg aagtccaagc tggtgtccga tttccggaag gatttccagt tttacaaagt
     5161 gcgcgagatc aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtgggaac
     5221 cgccctgatc aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg actacaaggt
     5281 gtacgacgtg cggaagatga tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa
     5341 gtacttcttc tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg
     5401 cgagatccgg aagcggcctc tgatcgagac aaacggcgaa accggggaga tcgtgtggga
     5461 taagggccgg gattttgcca ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt
     5521 gaaaaagacc gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa
     5581 cagcgataag ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcga
     5641 cagccccacc gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa
     5701 gaaactgaag agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga
     5761 gaagaatccc atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat
     5821 catcaagctg cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc
     5881 ctctgccggc gaactgcaga agggaaacga actggccctg ccctccaaat atgtgaactt
     5941 cctgtacctg gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa
     6001 acagctgttt gtggaacagc acaagcacta cctggacgag atcatcgagc agatcagcga
     6061 gttctccaag agagtgatcc tggccgacgc taatctggac aaagtgctgt ccgcctacaa
     6121 caagcaccgg gataagccca tcagagagca ggccgagaat atcatccacc tgtttaccct
     6181 gaccaatctg ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagag
     6241 gtacaccagc accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct
     6301 gtacgagaca cggatcgacc tgtctcagct gggaggcgac aagcgtcctg ctgctactaa
     6361 gaaagctggt caagctaaga aaaagaaagc tagcggcagc ggcgccacca acttcagcct
     6421 gctgaagcag gccggcgacg tggaggagaa ccccggcccc atgaccgagt acaagcccac
     6481 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     6541 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     6601 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     6661 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     6721 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     6781 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     6841 cgtcggagtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     6901 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     6961 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     7021 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga acgcgttaag tcgacaatca
     7081 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     7141 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     7201 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     7261 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     7321 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     7381 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     7441 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     7501 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     7561 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     7621 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca
     7681 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     7741 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     7801 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     7861 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     7921 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt
     7981 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     8041 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8101 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8161 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     8221 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     8281 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     8341 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     8401 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     8461 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     8521 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     8581 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     8641 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     8701 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     8761 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     8821 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     8881 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     8941 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     9001 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     9061 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     9121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     9181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     9241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     9301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     9361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     9421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     9481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     9541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     9601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     9661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     9721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     9781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     9841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     9901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     9961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
    10021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
    10081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
    10141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
    10201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
    10261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
    10321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
    10381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
    10441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
    10501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
    10561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    10621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    10681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    10741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
    10801 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
    10861 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
    10921 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
    10981 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
    11041 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
    11101 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
    11161 gattacgcca agcgcgcaat taaccctcac taaagggaac aaa
//
