LOCUS       sequence-211430-       11642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of Cas9-VQR (not codon
            optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11642)
  AUTHORS   Zafra MP, Schatoff EM, Katti A, Foronda M, Breinig M, Schweitzer AY,
            Simon A, Han T, Goswami S, Montgomery E, Thibado J, Kastenhuber ER, 
            Sanchez-Rivera FJ, Shi J, Vakoc CR, Lowe SW, Tschaharganeh DF, Dow 
            LE
  TITLE     Optimized base editors enable efficient editing in cells, organoids 
            and mice.
  JOURNAL   Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
  PUBMED    29969439
REFERENCE   2  (bases 1 to 11642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194."
COMMENT     Sequence Label: pLenti-VQR-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..11642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1726..1843
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1912..2123
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2143..2152
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2149..5550,5551..5553,5554..6150,6151..6153,6154..6156,
                     6157..6159,6160..6252)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKQYRSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6259..6279
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6286..6351
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        6367..6866
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6402..6425)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6799..6818
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6887..7486
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6887..6906)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7383..7403
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7502..8090
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7555..7575)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7973..7984)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8162..8395
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8473..8594
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8510..8529)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8564..8583
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8634..8769
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8789..8808)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8790..8808)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8818..8835)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8818..8834)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8827..8849)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8976..9431
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9063..9082)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9273..9294
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9457..9561
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9562..9630,9631..10422)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9780..9799)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10593..11181
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11082..11101
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11335..11352
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11469..11490
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11505..11510,11511..11528,11529..11535)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11543..11559
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11548..11570
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11567..11583
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11567..11583
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11602..11622
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11604..11622
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccagac cacaatttta aaagaaaagg
     1741 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1801 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1861 cagcagagat ccactttggc gccggctcga gtggctccgg tgcccgtcag tgggcagagc
     1921 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct
     1981 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
     2041 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
     2101 acgggtttgc cgccagaaca caggtgtcgt gacgcgggat ccgccaccat ggataaaaag
     2161 tattctattg gtttagacat cggcactaat tccgttggat gggctgtcat aaccgatgaa
     2221 tacaaagtac cttcaaagaa atttaaggtg ttggggaaca cagaccgtca ttcgattaaa
     2281 aagaatctta tcggtgccct cctattcgat agtggcgaaa cggcagaggc gactcgcctg
     2341 aaacgaaccg ctcggagaag gtatacacgt cgcaagaacc gaatatgtta cttacaagaa
     2401 atttttagca atgagatggc caaagttgac gattctttct ttcaccgttt ggaagagtcc
     2461 ttccttgtcg aagaggacaa gaaacatgaa cggcacccca tctttggaaa catagtagat
     2521 gaggtggcat atcatgaaaa gtacccaacg atttatcacc tcagaaaaaa gctagttgac
     2581 tcaactgata aagcggacct gaggttaatc tacttggctc ttgcccatat gataaagttc
     2641 cgtgggcact ttctcattga gggtgatcta aatccggaca actcggatgt cgacaaactg
     2701 ttcatccagt tagtacaaac ctataatcag ttgtttgaag agaaccctat aaatgcaagt
     2761 ggcgtggatg cgaaggctat tcttagcgcc cgcctctcta aatcccgacg gctagaaaac
     2821 ctgatcgcac aattacccgg agagaagaaa aatgggttgt tcggtaacct tatagcgctc
     2881 tcactaggcc tgacaccaaa ttttaagtcg aacttcgact tagctgaaga tgccaaattg
     2941 cagcttagta aggacacgta cgatgacgat ctcgacaatc tactggcaca aattggagat
     3001 cagtatgcgg acttattttt ggctgccaaa aaccttagcg atgcaatcct cctatctgac
     3061 atactgagag ttaatactga gattaccaag gcgccgttat ccgcttcaat gatcaaaagg
     3121 tacgatgaac atcaccaaga cttgacactt ctcaaggccc tagtccgtca gcaactgcct
     3181 gagaaatata aggaaatatt ctttgatcag tcgaaaaacg ggtacgcagg ttatattgac
     3241 ggcggagcga gtcaagagga attctacaag tttatcaaac ccatattaga gaagatggat
     3301 gggacggaag agttgcttgt aaaactcaat cgcgaagatc tactgcgaaa gcagcggact
     3361 ttcgacaacg gtagcattcc acatcaaatc cacttaggcg aattgcatgc tatacttaga
     3421 aggcaggagg atttttatcc gttcctcaaa gacaatcgtg aaaagattga gaaaatccta
     3481 acctttcgca taccttacta tgtgggaccc ctggcccgag ggaactctcg gttcgcatgg
     3541 atgacaagaa agtccgaaga aacgattact ccatggaatt ttgaggaagt tgtcgataaa
     3601 ggtgcgtcag ctcaatcgtt catcgagagg atgaccaact ttgacaagaa tttaccgaac
     3661 gaaaaagtat tgcctaagca cagtttactt tacgagtatt tcacagtgta caatgaactc
     3721 acgaaagtta agtatgtcac tgagggcatg cgtaaacccg cctttctaag cggagaacag
     3781 aagaaagcaa tagtagatct gttattcaag accaaccgca aagtgacagt taagcaattg
     3841 aaagaggact actttaagaa aattgaatgc ttcgattctg tcgagatctc cggggtagaa
     3901 gatcgattta atgcgtcact tggtacgtat catgacctcc taaagataat taaagataag
     3961 gacttcctgg ataacgaaga gaatgaagat atcttagaag atatagtgtt gactcttacc
     4021 ctctttgaag atcgggaaat gattgaggaa agactaaaaa catacgctca cctgttcgac
     4081 gataaggtta tgaaacagtt aaagaggcgt cgctatacgg gctggggacg attgtcgcgg
     4141 aaacttatca acgggataag agacaagcaa agtggtaaaa ctattctcga ttttctaaag
     4201 agcgacggct tcgccaatag gaactttatg cagctgatcc atgatgactc tttaaccttc
     4261 aaagaggata tacaaaaggc acaggtttcc ggacaagggg actcattgca cgaacatatt
     4321 gcgaatcttg ctggttcgcc agccatcaaa aagggcatac tccagacagt caaagtagtg
     4381 gatgagctag ttaaggtcat gggacgtcac aaaccggaaa acattgtaat cgagatggca
     4441 cgcgaaaatc aaacgactca gaaggggcaa aaaaacagtc gagagcggat gaagagaata
     4501 gaagagggta ttaaagaact gggcagccag atcttaaagg agcatcctgt ggaaaatacc
     4561 caattgcaga acgagaaact ttacctctat tacctacaaa atggaaggga catgtatgtt
     4621 gatcaggaac tggacataaa ccgtttatct gattacgacg tcgatcacat tgtaccccaa
     4681 tcctttttga aggacgattc aatcgacaat aaagtgctta cacgctcgga taagaaccga
     4741 gggaaaagtg acaatgttcc aagcgaggaa gtcgtaaaga aaatgaagaa ctattggcgg
     4801 cagctcctaa atgcgaaact gataacgcaa agaaagttcg ataacttaac taaagctgag
     4861 aggggtggct tgtctgaact tgacaaggcc ggatttatta aacgtcagct cgtggaaacc
     4921 cgccaaatca caaagcatgt tgcacagata ctagattccc gaatgaatac gaaatacgac
     4981 gagaacgata agctgattcg ggaagtcaaa gtaatcactt taaagtcaaa attggtgtcg
     5041 gacttcagaa aggattttca attctataaa gttagggaga taaataacta ccaccatgcg
     5101 cacgacgctt atcttaatgc cgtcgtaggg accgcactca ttaagaaata cccgaagcta
     5161 gaaagtgagt ttgtgtatgg tgattacaaa gtttatgacg tccgtaagat gatcgcgaaa
     5221 agcgaacagg agataggcaa ggctacagcc aaatacttct tttattctaa cattatgaat
     5281 ttctttaaga cggaaatcac tctggcaaac ggagagatac gcaaacgacc tttaattgaa
     5341 accaatgggg agacaggtga aatcgtatgg gataagggcc gggacttcgc gacggtgaga
     5401 aaagttttgt ccatgcccca agtcaacata gtaaagaaaa ctgaggtgca gaccggaggg
     5461 ttttcaaagg aatcgattct tccaaaaagg aatagtgata agctcatcgc tcgtaaaaag
     5521 gactgggacc cgaaaaagta cggtggcttc gtgagcccta cagttgccta ttctgtccta
     5581 gtagtggcaa aagttgagaa gggaaaatcc aagaaactga agtcagtcaa agaattattg
     5641 gggataacga ttatggagcg ctcgtctttt gaaaagaacc ccatcgactt ccttgaggcg
     5701 aaaggttaca aggaagtaaa aaaggatctc ataattaaac taccaaagta tagtctgttt
     5761 gagttagaaa atggccgaaa acggatgttg gctagcgccg gagagcttca aaaggggaac
     5821 gaactcgcac taccgtctaa atacgtgaat ttcctgtatt tagcgtccca ttacgagaag
     5881 ttgaaaggtt cacctgaaga taacgaacag aagcaacttt ttgttgagca gcacaaacat
     5941 tatctcgacg aaatcataga gcaaatttcg gaattcagta agagagtcat cctagctgat
     6001 gccaatctgg acaaagtatt aagcgcatac aacaagcaca gggataaacc catacgtgag
     6061 caggcggaaa atattatcca tttgtttact cttaccaacc tcggcgctcc agccgcattc
     6121 aagtattttg acacaacgat agatcgcaaa cagtacagat ctaccaagga ggtgctagac
     6181 gcgacactga ttcaccaatc catcacggga ttatatgaaa ctcggataga tttgtcacag
     6241 cttgggggtg acggatcccc caagaagaag aggaaagtct cgagcgacta caaagaccat
     6301 gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa gtaagatgca
     6361 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     6421 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     6481 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     6541 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     6601 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     6661 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     6721 gaaggggtgg gtccgggggc gggctcaggg gnnggctcag gggcggggcg ggcgcccgaa
     6781 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     6841 cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatga ccgagtacaa
     6901 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     6961 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     7021 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     7081 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     7141 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     7201 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     7261 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     7321 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     7381 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     7441 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgattct agaaagtcga
     7501 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     7561 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     7621 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     7681 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     7741 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     7801 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     7861 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     7921 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     7981 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     8041 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta
     8101 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     8161 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     8221 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     8281 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     8341 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg
     8401 tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     8461 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8521 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     8581 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     8641 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     8701 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     8761 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     8821 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     8881 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     8941 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     9001 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     9061 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     9121 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     9181 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     9241 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     9301 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     9361 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     9421 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     9481 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     9541 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     9601 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     9661 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     9721 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     9781 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     9841 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     9901 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     9961 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
    10021 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
    10081 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
    10141 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
    10201 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
    10261 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
    10321 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
    10381 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
    10441 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
    10501 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    10561 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    10621 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    10681 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
    10741 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    10801 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    10861 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    10921 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    10981 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    11041 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    11101 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    11161 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    11221 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
    11281 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
    11341 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
    11401 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
    11461 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
    11521 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
    11581 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg
    11641 ca
//
