LOCUS       sequence-211440-        5956 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Foamy virus helper plasmid expressing Gag, driven by a CMV promoter.
            Ampicillin resistant..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Josephson NC, Trobridge G, Russell DW
  TITLE     Transduction of long-term and mobilized peripheral blood-derived 
            NOD/SCID repopulating cells by foamy virus vectors.
  JOURNAL   Hum Gene Ther. 2004 Jan;15(1):87-92.
  PUBMED    14965380
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther."; date: "2004-01"; volume: "15(1)"; pages: "87-92"
COMMENT     Sequence Label: pCiGS-Delta-Psi
FEATURES             Location/Qualifiers
     source          1..5956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..330
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        331..534
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     485..505
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     531..555
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          672..768
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     regulatory      839..848
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3095..3229
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3119..3138)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3173..3192
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3340..3356)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3340..3356)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3353..3375)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3364..3380
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3388..3394,3395..3412,3413..3418))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3433..3454
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3571..3588)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3742..4330)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3822..3841)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4501..5292,5293..5361))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5124..5143
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5362..5466)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5534..5552
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5590..5612)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5712..5731
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5925..5947
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5939..5956
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5940..5956
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tcgaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg gcccgcccaa cgacggccgc
      841 caccatggct tcaggaagta atgttgaaga atatgaactt gatgttgaag ctctggttgt
      901 aattttaaga gatagaaata taccaagaaa tcctttacat ggagaagtta taggtcttcg
      961 ccttactgaa ggatggtggg gacaaattga gagatttcag atggtacgtc taatattaca
     1021 aaatgatgat aatgaacctt tacagagacc tagatatgag gtaatacaac gagctgtaaa
     1081 ccctcataca atgtttatga tatcaggacc attagctgaa cttcaattag cctttcagga
     1141 tttagattta cctgaaggtc cattgaggtt tggtccattg gcaaatggac attatgttca
     1201 aggagatcct tatagtagtt cttacagacc agtaacaatg gccgaaacag cccaaatgac
     1261 tagagatgaa ctggaagatg ttcttaatac tcaaagtgaa atagaaattc aaatgataaa
     1321 tttattggag ttgtatgaag ttgaaactag agctcttaga agacaattag ctgagagatc
     1381 tagtacaggg caaggaggaa tatccccagg agctcctcgt tctcgaccac cagtaagcag
     1441 cttctcaggg ttaccaagtt tgccctctat acctgggatt catcccaggg caccttcacc
     1501 tccaagggca acttctactc cgggaaatat tccttggagt ttaggagatg ataacccacc
     1561 ttcatctagt tttcctggac cctctcaacc tcgtgtttct ttccatccgg gaaatccttt
     1621 tgttgaagaa gaaggtcata gacctagatc ccagtctaga gaaaggagaa gagaaattct
     1681 tcctgctcct gtaccgtcag cacctcctat gattcagtat ataccagtac cacctccacc
     1741 accgattggc acggttatac ctattcagca tatcagatct gtaactggag agcctcctag
     1801 aaacccaaga gaaataccaa tttggctagg acgaaatgct cctgctatag atggagtgtt
     1861 ccctgttaca acaccggatc taagatgcag aataattaat gctatactag gaggaaatat
     1921 tgggctatca ttaacccctg gagactgttt aacatgggac tcagcagtag ccaccttatt
     1981 tattagaacc catggaactt ttccaatgca tcagcttgga aatgtaataa aaggcatagt
     2041 tgatcaagaa ggagtggcaa cagcatatac tttgggaatg atgctttctg gacaaaatta
     2101 tcaattagtt tctggaataa ttagaggata tttgcctgga caagctgtag taactgcatt
     2161 acaacagcgt ttagaccaag aaatagatga tcaaacaaga gcagagactt ttattcaaca
     2221 tctaaatgct gtatatgaaa ttttaggcct taatgccaga ggacaaagta tacgtgcttc
     2281 agtgactcct caaccccgac catccagagg tagaggtcga ggtcaaaata cttctagacc
     2341 ctctcaagga ccagctaata gcgggcgggg acgacagcgc cctgcttctg gtcaaagcaa
     2401 cagaggatct agtactcaga atcaaaatca agataattta aatcaaggag gatataatct
     2461 tcgaccccgt acttaccaac ctcaaaggta cggaggagga cgtggacgaa gatggaacga
     2521 taatactaac aatcaagagt ccagaccatc agatcaaggt tctcaaactc ctaggccaaa
     2581 tcaagcaggc tctggggtgc gtggcaatca gtcacaaact cccagaccag ctgctggtcg
     2641 cggaggaaga ggtaaccaca accgaaacca acgatcatcc ggtgctggtg actcacgcgc
     2701 tgtcaatacc gtgacacaga gtgccacgtc ctccacagat gaatcctctt cagctgttac
     2761 agccgcttcc ggcggagatc aaagggacta aattgttagc ccactgggat tcaggggcaa
     2821 caataacttg tattcctgaa agttttttag aagatgaaca acctattaaa aagactttaa
     2881 taaaaacaat tcatggagaa aaacaacaaa atgtttatta tgtaaccttt aaagttaaag
     2941 gaagaaaagt ggaagcagaa gtgatagctt ctccttatga gtatattttg ctgtcgccaa
     3001 cagatgttcc ttggttaaca cagcaaccac ttcagttaac aattttagtt cctcttcaag
     3061 aatatcaaga gaaaatctta attgcggccg cggggatcca gacatgataa gatacattga
     3121 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     3181 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     3241 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt cggatcctct
     3301 agagtcgacc tgcaggcatg caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     3361 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     3421 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     3481 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     3541 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     3601 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     3661 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     3721 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     3781 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3841 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     3901 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     3961 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4021 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4081 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4141 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     4201 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     4261 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     4321 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     4381 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     4441 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     4501 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     4561 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     4621 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     4681 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     4741 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     4801 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     4861 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     4921 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     4981 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     5041 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     5101 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     5161 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     5221 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     5281 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     5341 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     5401 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     5461 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     5521 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     5581 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     5641 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     5701 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     5761 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
     5821 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     5881 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     5941 taaaacgacg gccagt
//
