LOCUS       sequence-211443-       14371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14371)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 14371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU74.1-2.2
FEATURES             Location/Qualifiers
     source          1..14371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..1095
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(1342..1358)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1342..1358)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             1869..1892
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(10160..10180)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10160..10178)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(10517..11105)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10597..10616)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11276..12067,12068..12136))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11899..11918
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12137..12241)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      12268..13610
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(13231..13278)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     13651..13669
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        13964..14371
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
ORIGIN
        1 atgtctgccc ctaagaagat cgtcgttttg ccaggtgacc acgttggtca agaaatcaca
       61 gccgaagcca ttaaggttct taaagctatt tctgatgttc gttccaatgt caagttcgat
      121 ttcgaaaatc atttaattgg tggtgctgct atcgatgcta caggtgttcc acttccagat
      181 gaggcgctgg aagcctccaa gaaggctgat gccgttttgt taggtgctgt gggtggtcct
      241 aaatggggta ccggtagtgt tagacctgaa caaggtttac taaaaatccg taaagaactt
      301 caattgtacg ccaacttaag accatgtaac tttgcatccg actctctttt agacttatct
      361 ccaatcaagc cacaatttgc taaaggtact gacttcgttg ttgtcagaga attagtggga
      421 ggtatttact ttggtaagag aaaggaagac gatggtgatg gtgtcgcttg ggatagtgaa
      481 caatacaccg ttccagaagt gcaaagaatc acaagaatgg ccgctttcat ggccctacaa
      541 catgagccac cattgcctat ttggtccttg gataaagcta atgttttggc ctcttcaaga
      601 ttatggagaa aaactgtgga ggaaaccatc aagaacgaat tccctacatt gaaggttcaa
      661 catcaattga ttgattctgc cgccatgatc ctagttaaga acccaaccca cctaaatggt
      721 attataatca ccagcaacat gtttggtgat atcatctccg atgaagcctc cgttatccca
      781 ggttccttgg gtttgttgcc atctgcgtcc ttggcctctt tgccagacaa gaacaccgca
      841 tttggtttgt acgaaccatg ccacggttct gctccagatt tgccaaagaa taaggtcaac
      901 cctatcgcca ctatcttgtc tgctgcaatg atgttgaaat tgtcattgaa cttgcctgaa
      961 gaaggtaagg ccattgaaga tgcagttaaa aaggttttgg atgcaggtat cagaactggt
     1021 gatttaggtg gttccaacag taccaccgaa gtcggtgatg ctgtcgccga agaagttaag
     1081 aaaatccttg cttaaaaaga ttctcttttt ttatgatatt tgtacataaa ctttataaat
     1141 gaaattcata atagaaacga cacgaaatta caaaatggaa tatgttcata gggtagacga
     1201 aactatatac gcaatctaca tacatttatc aagaaggaga aaaaggagga tgtaaaggaa
     1261 tacaggtaag caaattgata ctaatggctc aacgaaatga cggtatcgat aagcttgata
     1321 tcgaattcct gcagcccggg ggatccacta gttctagatt aattaatatc taaaaattgc
     1381 cttatgatcc gtctctccgg ttacagcctg tgtaactgat taatcctgcc tttctaatca
     1441 ccattctaat gttttaatta agggattttg tcttcattaa cggctttcgc tcataaaaat
     1501 gttatgacgt tttgcccgca ggcgggaaac catccacttc acgagactga tctcctctgc
     1561 cggaacaccg ggcatctcca acttataagt tggagaaata agagaatttc agattgagag
     1621 aatgaaaaaa aaaaaaaaaa aaaggcagag gagagcatag aaatggggtt cactttttgg
     1681 taaagctata gcatgcctat cacatataaa tagagtgcca gtagcgactt ttttcacact
     1741 cgaaatactc ttactactgc tctcttgttg tttttatcac ttcttgtttc ttcttggtaa
     1801 atagaatatc aagctacaaa aagcatacaa tcaactatca actattaact atatcgtaat
     1861 acacaatgga ttataaggat gatgatgata agactagtat ggcatcaggt gttgctattg
     1921 ttggcatctc cgtagagctg cccagtggga actattccga ggagaattta gaccataatt
     1981 ctttcttcga attcctcctg aattacggcg aatcctacga acggcttcct cgagatcggt
     2041 tcaatgtcga agcttggaga ggtaacggta tcgggcaaat ccacgtagac gggggcacgt
     2101 tcctcaagga aatcgactcg tttgacaaca tcgagttcgg agtttcgtcg cgagatgcac
     2161 gcgcaatggc gccggtcacc aagaaactgt tggagaattg cttcctcgcg cttctcgact
     2221 caggaatcga ctatcgtgca cggaacgtgg gctgctacac cgccggaaat tcgttcgagc
     2281 tgtcaaatat cactgaacca gatgagtacg aaccgcaagg atcgttcgcg ggatacgcgg
     2341 caatggtggc aaaccgcgtc tcgaaccatc tcgacttgct tggaccgtcc ctacctacgg
     2401 acactgcatg tagctccacg ctcgtagcgc tgcatctagc tgtacaggct gtgctgaatg
     2461 gcgattgtga ggctgccgtt gtaggtggct gccagctcaa ccatcgcctc atggattgga
     2521 tcacctatag tcaaggctcc ctgctcgcga aggatggcaa gtgcaagccc ttcgacgcgt
     2581 ccgcggatgg gttcgcaaga gccgaaggtt gtgtcgctgt cgtgctcaaa acactcgaag
     2641 atgctctgcg ggacaatgat tatatttatg ctacaattct tgggacagcg atcaataatg
     2701 gcggcgctgg agcaccacct ggggcaccag ttgcggacgc ccaacgtgat gctatgttgc
     2761 aagcgtttcg tcgcgccgga agatgtccta aggacgtcga ttatatcgag ctacatgcaa
     2821 ccgggaccgc gaaaggtgat cccacagagg ccaattgggt tggcaaagag ttccagaggg
     2881 aagacgaggt gttgatcggc agtgtcaagg ggaatatagg gcacacggag ataacagcct
     2941 tcctggcgtc cttatccaag gtgctctcaa ttttgcaacg tggcaccatc cctccaaacg
     3001 tgaacctcac cgaccctaat cccgagatca agtgggcaga gtacaggctg aaggtaccga
     3061 cgagcccaat gctgcttccc cgccattcgc ccaacacgca cctcgtctcg atggctggct
     3121 cgggcatcgg aggctcgaac ggccacgtcg tcctagaggg cccgcctctg cgagcacccg
     3181 tgggtgcgca gcaacaccta aaggcgcgcc gtccggtgct gctccttgct ggcgggctca
     3241 ccccgcgaag tgcagctgca gtagcggagt cgatcattgc gtgcatcgaa ggcggcgagg
     3301 tcgacgtgtc tgcgatgtcg acgatcctcg ggcgccgtgc cagacagatg acgtggaggt
     3361 cgtacgtagt ctccggcggg gacacatcca attcgatcag ctttggcgag ccccagcatt
     3421 gcccacgcaa tgcaaatccg ctcgtgttcg tcttctcggg tcaaggtccc cagcacatca
     3481 acatggggcg cgaactcttc aagacatttc ctgtgttccg gaacagcgtg ctgaacatgg
     3541 acaacaccta caagaccgcc actggaaagt ctatcatcga cgactggggc ttgttcggcg
     3601 ggaactgctc aacagcactg ccagatgtgt ggccgatctc cctcaccctg ccagccatag
     3661 caatcttcca gatcgcatta ttcgacctcc ttgtccatct cggcatcaac cccgacattg
     3721 tcattgggca ctcagccggc gagacggccg tgctctacgc gtcgggcgct gcgccgaagg
     3781 caatgacggt cgaattggcc gttctgcggg gcgttatctt cacccaattg gaacaccagg
     3841 gaggtaccat ggccgcgcta tcctgcactg cgcacgaagc gatgcagata ctggaccagg
     3901 cctgtgcgca ggagccgggg tgcgcggttg agattgcctg ctataatgcg ccggcggcgg
     3961 tcgccatcgc cgggcacatg cgcgcggttg atcgcgttgt agagcttgcg caggcccgtg
     4021 ggatatacat acgcaagctt cctactcggg tgccgatcca ttcgtcactg atggatgcgt
     4081 gcagggaaga gtattgcggt ggcttgtatg agctgttcgc acgttaccct ggggcgcatg
     4141 tcccaaagat tctgacctac tcgacgctta cgggagggcc gtttgggggc ccattcgatg
     4201 ccgactactt ttggcagaac acgcgcggtg aagtgcgctt caccgaaaca gtggaagcga
     4261 ttgcttcgta cggaagtcat actttcgtag agatctctcc acgacctgct ttggcatcct
     4321 acgtccggtc catggcgcaa gagccgaaca ttgtcctcac caccgtgcgc cggcagaggt
     4381 cgggacagga cccatcggaa tatcgcgacg tcctcgaact ctgcgggcaa ctcactatta
     4441 ctgggcataa ttgcgttgac tttacggtac tcaacggtcg agcgtgctat gaagtacagg
     4501 tccctttacc ttcttatcca ttctcgaaga agcgattccc gttgtatcct gacactgccg
     4561 gctacagaaa acagcttgag cctcgtcgag gacctttgaa ccatagacat ctccgcgtca
     4621 acaaggagtc acatccctca ctgggtgaac atgtcattag aggcgagccg ataatgcccg
     4681 ctgccgggtt cattgagatg gccctagaat ttggagcacg cacactcatc aatgtcaacc
     4741 tgcaatctat cctgtcgctt tctgcggaaa ggccagtatc gttacaagtt cgactccaag
     4801 gctcattttg gactgtatca tccacggttc caacgtccaa gatctcctca aatatagact
     4861 cagatcatat cgaacgacta catgccgatg gattcctttc ctttgaagca gtaccaacat
     4921 atcccgatct cgatgtatct ggactccgga aacatcttca gaacaatgtc ggctcagatt
     4981 tctatccctc actttcctac tattcttcat atggtccgag gatgcgacgc gtgacgaacg
     5041 tatactttgg ccgaaacgag gcactggcat caatctgcgg aatggatgac atactctccg
     5101 aggatgccgc ctatttctta caccctgcaa ttctggatgc ttgcatacaa gtctccgcat
     5161 acaagccttt ccacggcaat ttcgacccca acgtttacta tctaccagct catgttgacg
     5221 cagtcttcgt ccatgagcca cttaagacca gctattttcc tgcccatcta tacgctcatg
     5281 ttgaattgag ggcgtggcat cccgaccata tgcgatacga cgtctccctc gtgaacgatt
     5341 ttgggaagag gctatgtacg gtcaaaggct tgagaatgac aaaacaccac atcaatccgt
     5401 tggcaagtgt ctccggccct ctggagcttg ttcttcagcc tatttccatc ctatcacttc
     5461 cgacgacatc tggaaggatc tccactgcac atcaaaacac ccagctcttc gagatatctg
     5521 acgaaataga gctgcaccat caataccgaa ccaatggggt acacgactgc tccgatgtcc
     5581 agtattggtg ccatagccgt gacattcaag ctcttaatct agagagtcaa agtggactct
     5641 cctcacacga ggacacgacc ggctccgtaa ttgaaggggt gttgcacacc attttgagcc
     5701 acatatccga tcaatcacat atacgtgtga tccgggtctt gatctctcgc ttgcatccca
     5761 taatgctcga atctttcgct caggtcttga aggggttccc ggctgtcttc ttcgagatat
     5821 acatcagctc ggacgacaca agtgcgacgc ttctagtaga agacttctca gggattgttc
     5881 gcactgctgc gactgacatc cagcgctcat ttttcgaaat gatcttcaca tcgccaaatg
     5941 acttcccaga ggcagtgata cagtcatttg gtagcatgtt agtacctgga ggcgtgattg
     6001 tatttgccga aggccttcct aagcctctgg gccacagata cctcagcctc gacaccgtcc
     6061 gacacgtgtc atcaacaccc acactcccac tcgaaacgct ggagaagttg ggtttttctg
     6121 tggtacagcc ccatggcccc gactgtgcta ttgcgcagaa ttgtaccgcc gatgctctcc
     6181 aagccaccga acccttatat gatccactgg aagcattcgt attccaatac gaactcagaa
     6241 acgaggtcga attgcagtgg gaatttagcg ggctcaacat taggcaagag ctggatgtct
     6301 ggatcctcgc ttcggaagga aaagatggtg gatctgcggt gggaatggta cgggccctga
     6361 gaagggagta ttcatcgtgg acaatccgcc ttgtcgtctt cccactctcg tacccggaag
     6421 agatgcggct ccaatgtctt cgccgacttc ccacctacat gaaagaggaa ctagacattc
     6481 tcgtatcccg tacaggagac ttcacagtct ttcgattggc ccctgtcggc aggccgcccc
     6541 atgatcacaa tcctgcccgc tggaacgaca cgaccagtga tatcccccac ggacatgttc
     6601 tcgtacatgt tctgtattcg tccagccact acgatatttc agcattggtg gcgtccgtag
     6661 acaatgccaa caataccggt atccgagagg gatccatact attggccatg attacaggta
     6721 cccccggaac gtcgctcgtc ttggatgtta ctatcacttg ctcagtaccg ccatctctcc
     6781 ttgagcaagt atcgatggtt gtgcaaatga tggccccgct cgtgacggct gtccttgctc
     6841 ccggtctagc cgccttcagg tcgccagctc gtcttcgaac attacgaatc ttattgactc
     6901 atgctgatat cccatttggg cgaatcataa agagtatcta tgcggaaaag gcgacactca
     6961 ctgaagtagt cgagaatatc actctctttg atctagcgtc actcgagagt cagccatttg
     7021 acttgatcat atccggatac gacgatcacg cccacagtca agtcctccgt tccatactca
     7081 gacccggccg cggaagactg tttttgtggg gcaatcgtca aggtctccgc gaactcatag
     7141 atagagagct atacacagtt gtggatgcca tgaactgcgt tcttcagtct cttgacgagc
     7201 aaccccgcat catatgcgaa aacacatcaa ttaccccttc attggcgcca tccgatgtca
     7261 tcgtgacaca agtcagccca caggccatat ccactggagt caccttcagt gccgacaaaa
     7321 catatctgct tgtaggaggc attgggagca ttggagcgcg actggcactc tacttgtacg
     7381 agagaggggc tcgacatatt gttgtctcat cgcgtaccgg ggcaaggggc cttcgcacaa
     7441 gtaacaatct tatggtgtat cgaatattcg aatatctcaa ggggatcagc gacctggaca
     7501 tccgcctcgt tgccgtagac gcatcttcac gacacgaaat gaagacgttg cgtgacaaca
     7561 tcaatccgaa aatcgccgga tgtatcatcc tcactgctgt actctccgac cgtgtgttcc
     7621 aatacctcga ggaagagcag tttactgccg ttttcgctgc taagattgga gttctcgatg
     7681 ctcttattga cacgatagac gtatcgagtc tcgagtttct cgttgccttt tcttccgtct
     7741 caggcttgtt cggtgtcggt ggtcagtcga attacggcgc agcgaattca actctagagg
     7801 agacgatgtc agtaatctct aacgggttct gcttcgtgtg ccctgggatt ctcgactcgt
     7861 ccttgatgct ggctaatggg atggataccg agaagcatcg ccttgctcac ttcatagagt
     7921 ggtctatcac aacagatgaa atggttctgt ggttcgatga cgcgatgacg agataccagg
     7981 gtggccaaaa gttcagtcgt tacatcccca atctgaattg ggaggtgatg gaccgcacgc
     8041 acggaatgaa caaactggga gcacatctag ttccgacgcg cacggctaag gcacctacta
     8101 cggaggacga cacaggtcga gtggcagaga ttgtcagaag caccttgaat atatcctcag
     8161 acgacttctc cgcagaagta cctttcaccg catacggtct agactcgctc tccgcttcaa
     8221 gattgtcctt catgctgcgt cctatgatga gcgttaccca gatacaactc ctagcggata
     8281 tgaccctcag agatctccag actagactgt ccgccgaacc agtgtcggag gattttcggg
     8341 ccctagagcc gaccacgggc aagaatgaaa tacaagcgat gcgcgacatg ctcagcaaat
     8401 atacgcagga tttacgggaa caaagagcta caaccgacat ccgagcgacg cgcaacgatg
     8461 ttgtcctcct cacaggaagc actggcgctc tcggtgccaa cattcttgct cagctcctcg
     8521 acaacagcga tgtcacgaag gttttcgccc tgaataggaa aggcactgat cgccattgcc
     8581 tgattgagcg acaagtggct gccctcgtga cacagggcct gccgcctacg ttggccgaat
     8641 cgcagaaact ggtgctgatc gaaggagatt tactgctgga tggacttggt attccactag
     8701 aggtgtttag tgagatgcta tcctcagtga cacacgtaat ccacaatgcc tggaccatcg
     8761 acttcttgtc ctctctggag gcaatggaag atcttgtaag gggcacccgt cggctattgg
     8821 acattgctgc tctctcgact cgtgcgactc ctccatcatt ttcctttatc agttctattg
     8881 gcatttacca gcattattcg ggttcggctc cagcaccaga ggagcccgtg ctcgatgcga
     8941 agatggccgt acagaccggt tacatggagt ccaaatgggt ggccgaacga attgttcaga
     9001 tagctggcgc tagactcggg ctcaaagcgc acgtgattcg agtcggactc ctgacaggag
     9061 gcgtcaatgg ctcatgggat cctacacact ggctcccagc gctcgtgcag tctgcaacgt
     9121 acgtagggtg cctcccagaa agcgatagcc tcgtctcatg gatcccggtg gacgtcgctg
     9181 ctgctgcggt agttgatcta cgccacgcct ctgatgatac actccatgtg attcaccctc
     9241 gcccgatgct ttggtcagac atcatggttc ccatagcatc gctgctcgat gttccacttg
     9301 ttccctacat cgaatggttc tgtcgactcg agaacatggc caacgcaatc tcccatgcac
     9361 ctagtcagcg agacagccgg cggctgaggc acgcgctcag acttattgat ttttatcggc
     9421 tgggaatgcg aggcagtaag ggagtttctg ggtccacgga gagcatgggt ctattgccgc
     9481 aagtgaaatg tcagaaggca atgcgaagtt ctgcgacact gcagaatccc gaactatgtc
     9541 aattggggat cagcgacatc gaacgatggg tgtcttattg gcgacaagcc ggtcatctgc
     9601 caaataatga ctcgccaaac tgaggagatt gataagactt ttctagttgc atatctttta
     9661 tatttaaatc ttatctatta gttaattttt tgtaatttat ccttatatat agtctggtta
     9721 ttctaaaata tcatttcagt atctaaaaat tcccctcttt tttcagttat atcttaacag
     9781 gcgacagtcc aaatgttgat ttatcccagt ccgattcatc agggttgtga agcattttgt
     9841 caatggtcga aatcacatca gtaatagtgc ctcttacttg cctcatagaa tttctttctc
     9901 ttaacgtcac cgtttggtct tttatagttt cgaaatctat ggtgatacca aatggtgttc
     9961 ccaattcatc gttacgggcg tattttttac caattgaagt attggaatcg tcaattttaa
    10021 agtatatctc tcttttacgt aaagcctgcg agatcctctt aagtatagcg gggaagccat
    10081 cgttattcga tattgtcgta acaaatactt tgatcggcgc tatgcggccg ccaccgcggt
    10141 ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg agactcaaac
    10201 aagatatatt ttgcccgcct tacagacgaa actagttgga ggttatggag catactatca
    10261 cgtgggcggc cactggtgag ttactacacc ccaggggcaa cgttgatgct cctaaaaaac
    10321 tctggctgga cgcaagccgt aacacccgtg tcacttcata atcgtttgca attcagggct
    10381 tgatctacac attaccctgt tatccctatt aattaatacg gttatccaca gaatcagggg
    10441 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    10501 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    10561 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    10621 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    10681 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    10741 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    10801 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    10861 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    10921 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
    10981 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    11041 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    11101 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    11161 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    11221 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    11281 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    11341 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    11401 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    11461 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    11521 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    11581 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    11641 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    11701 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    11761 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    11821 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    11881 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    11941 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    12001 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    12061 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    12121 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    12181 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    12241 gcacatttcc ccgaaaagtg ccacctgaac gaagcatctg tgcttcattt tgtagaacaa
    12301 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
    12361 cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc atttttgtaa
    12421 aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta
    12481 cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat acttcttttt
    12541 tgttctacaa aaatgcatcc cgagagcgct atttttctaa caaagcatct tagattactt
    12601 tttttctcct ttgtgcgctc tataatgcag tctcttgata actttttgca ctgtaggtcc
    12661 gttaaggtta gaagaaggct actttggtgt ctattttctc ttccataaaa aaagcctgac
    12721 tccacttccc gcgtttactg attactagcg aagctgcggg tgcatttttt caagataaag
    12781 gcatccccga ttatattcta taccgatgtg gattgcgcat actttgtgaa cagaaagtga
    12841 tagcgttgat gattcttcat tggtcagaaa attatgaacg gtttcttcta ttttgtctct
    12901 atatactacg tataggaaat gtttacattt tcgtattgtt ttcgattcac tctatgaata
    12961 gttcttacta caattttttt gtctaaagag taatactaga gataaacata aaaaatgtag
    13021 aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt atatagggat
    13081 atagcacaga gatatatagc aaagagatac ttttgagcaa tgtttgtgga agcggtattc
    13141 gcaatatttt agtagctcgt tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt
    13201 cagagcgctt ttggttttca aaagcgctct gaagttccta tactttctag agaataggaa
    13261 cttcggaata ggaacttcaa agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga
    13321 gctgcgcaca tacagctcac tgttcacgtc gcacctatat ctgcgtgttg cctgtatata
    13381 tatatacatg agaagaacgg catagtgcgt gtttatgctt aaatgcgtac ttatatgcgt
    13441 ctatttatgt aggatgaaag gtagtctagt acctcctgtg atattatccc attccatgcg
    13501 gggtatcgta tgcttccttc agcactaccc tttagctgtt ctatatgctg ccactcctca
    13561 attggattag tctcatcctt caatgctatc atttcctttg atattggatc atactaagaa
    13621 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
    13681 gcgcgtttcg gtgatgacgg tgagcgcgtt tcggtgatga cggtgaaaac ctcggcgcgc
    13741 cattaccctg ttatccctaa tatacaacgc aaagaagctg gaaaattggc gagggaatcc
    13801 tgtttctgtc tatccaagaa tgggcatgag gtggcaaccg tcgtgctagc gtacagggtg
    13861 cactttgtaa ccatttggga caccggacac tcgctgtttt cgaaattacc ctttaagcgc
    13921 gggtattgaa ccaggcttat gcccaagatc gtagcaagca tttaactgtg ggaatactca
    13981 ggtatcgtaa gatgcaagag ttcgaatctc ttagcaacca ttattttttt cctcaacata
    14041 acgagaacac acaggggcgc tatcgcacag aatcaaattc gatgactgga aattttttgt
    14101 taatttcaga ggtcgcctga cgcatatacc tttttcaact gaaaaattgg gagaaaaagg
    14161 aaaggtgaga gcgccggaac cggcttttca tatagaatag agaagcgttc atgactaaat
    14221 gcttgcatca caatacttga agttgacaat attatttaag gacctattgt tttttccaat
    14281 aggtggttag caatcgtctt actttctaac ttttcttacc ttttacattt cagcaatata
    14341 tatatatata tttcaaggat ataccattct a
//
