LOCUS       sequence-211473-        6802 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Foamy virus helper plasmid expressing Env, driven by a CMV promoter.
            Ampicillin resistant..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Trobridge G, Josephson N, Vassilopoulos G, Mac J, Russell DW
  TITLE     Improved foamy virus vectors with minimal viral sequences.
  JOURNAL   Mol Ther. 2002 Sep;6(3):321-8.
  PUBMED    12231167
REFERENCE   2  (bases 1 to 6802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther.";
            date: "2002-09"; volume: "6(3)"; pages: "321-8"
COMMENT     Sequence Label: pCiES
FEATURES             Location/Qualifiers
     source          1..6802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..330
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        331..534
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     485..505
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     531..555
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          672..768
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    3941..4075
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3965..3984)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4019..4038
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4186..4202)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4186..4202)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4199..4221)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4210..4226
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4234..4240,4241..4258,4259..4264))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4279..4300
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4417..4434)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4588..5176)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4668..4687)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5347..6138,6139..6207))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5970..5989
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6208..6312)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6380..6398
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6436..6458)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6558..6577
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6771..6793
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6785..6802
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6786..6802
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tcgaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg gccgcaatcg agtctagacc
      841 cttaaggtgt tgaatccaag gactgttgtt attttggacc atcttggcaa caacagaact
      901 gtaagtatag ataatttaaa acctacttct catcagaatg gcaccaccaa tgacactgca
      961 acaatggatc atttggaaaa aaatgaataa agcgcatgag gcacttcaaa atacaacaac
     1021 tgtgactgaa cagcagaagg aacaaattat actggacatt caaaatgaag aagtacaacc
     1081 aactaggaga gataaattta gatatctgct ttatacttgt tgtgctacta gctcaagagt
     1141 attggcctgg atgtttttag tttgtatatt gttaatcatt gttttggttt catgctttgt
     1201 gactatatcc agaatacaat ggaataagga tattcaggta ttaggacctg taatagactg
     1261 gaatgttact caaagagctg tttatcaacc cttacagact agaaggattg cacgttccct
     1321 tagaatgcag catcctgttc caaaatatgt ggaggtaaat atgactagta ttccacaagg
     1381 tgtatactat gaaccccatc cggaacccat agtggtgaag gagagggtcc tgggtctttc
     1441 tcaaattctg atgattaatt cagaaaacat tgctaataat gctaatttga cacaagaagt
     1501 aaagaagttg ttaactgaaa tggttaatga agaaatgcaa agtttgtcag atgtaatgat
     1561 tgactttgaa attcctttag gagaccctcg tgatcaagaa caatatatac atagaaaatg
     1621 ctatcaagaa tttgcaaatt gttatttagt aaaatataaa gaacccaaac cgtggcctaa
     1681 ggagggcctt atagctgatc aatgcccatt accaggttac catgctggat taacctataa
     1741 tagacagtct atttgggatt actatattaa agtggagagt attagacctg caaattggac
     1801 aacaaagagt aaatatggac aagctagact aggaagtttt tatattccta gcagtctgag
     1861 acaaatcaat gttagtcatg tactattctg tagtgatcaa ttatattcta aatggtataa
     1921 tatagaaaat accatagaac aaaacgagcg gtttctgctt aataaactaa ataaccttac
     1981 atctggaacc tcagtattga agaaaagagc tcttccgaag gattggagtt ctcaaggtaa
     2041 aaatgctctg tttagagaaa tcaatgtgtt agatatctgc agtaaacctg aatctgtaat
     2101 actattgaat acttcatact attccttctc tttatgggaa ggagattgta attttactaa
     2161 agatatgatt tctcagttgg ttccagaatg tgatggattt tataacaatt ctaagtggat
     2221 gcatatgcat ccatatgctt gtagattctg gagaagtaag aatgaaaaag aagaaactaa
     2281 atgtagagat ggggaaacta agagatgtct gtattatcct ttatgggaca gtcccgaatc
     2341 tacatatgat tttggttatt tagcatacca aaagaatttt ccttccccta tctgtataga
     2401 acaacagaaa attagagatc aagattatga agtttattct ttgtatcaag aatgcaaaat
     2461 agcttctaaa gcatatggaa ttgatacagt tttattctct ctaaagaatt ttcttaatta
     2521 tacaggaact cctgtaaatg aaatgcctaa tgcaagagct tttgtaggcc taatagatcc
     2581 caagtttcct ccttcctatc ccaatgttac tagggaacat tatacttcct gtaataatag
     2641 gaaaagaaga agtgttgata ataactatgc taagttaagg tctatggggt atgcacttac
     2701 aggagcagtg caaaccttat ctcaaatatc agatattaat gatgaaaact tacagcaagg
     2761 aatatattta ttaagggatc atgtaataac cttaatggaa gctacattgc atgatatatc
     2821 tgttatggaa ggaatgtttg ctgtacaaca tttgcataca catttgaatc atttgaagac
     2881 aatgcttcta gaaagaagaa tagactggac ctatatgtct agtacttggc tacaacaaca
     2941 attacagaaa tctgatgatg agatgaaagt aataaagaga attgctagaa gtttggtata
     3001 ttatgttaaa caaacccata gttctcccac agctacagcc tgggagattg gattatatta
     3061 tgaattggtt atacctaaac atatttactt gaataattgg aatgttgtca atataggtca
     3121 cttagttaaa tcagctggac aattgactca tgtaactata gctcatcctt atgaaataat
     3181 caataaggaa tgtgtagaga ctatatatct gcatcttgaa gactgcacaa gacaagatta
     3241 tgtcatatgt gatgtggtaa agatagtgca gccttgtggc aatagctcag acacgagtga
     3301 ttgtcctgtc tgggctgaag ctgtaaaaga accatttgtg caagtcaatc ctctgaaaaa
     3361 cggaagttat ctggttttgg caagttccac agactgtcag atcccaccat atgttcctag
     3421 catcgtgact gttaatgaaa caacgtcatg ctttggactg gactttaaaa ggccactggt
     3481 tgcggaagaa agattgagct ttgagccacg actgccaaat ctacaactaa gattaccaca
     3541 tttggttgga attattgcaa aaatcaaagg gataaaaata gaagtcacat cctctggaga
     3601 aagtataaaa gagcagattg aaagagcaaa agctgagctc cttcgactgg acattcacga
     3661 gggagatact cctgcctgga tacaacagct agctgcagca acaaaggacg tctggccagc
     3721 agcagcttct gctctacaag gaattggtaa ctttttatct gggactgccc aaggaatatt
     3781 tggaactgcc tttagtctct tgggatactt aaagcctatc ctaataggag taggggtcat
     3841 tctcttggtt attcttatat ttaagattgt atcatggatt cctacgaaaa agaagaatca
     3901 gtagcctcca cctctggaat tcggatcttg cggccgcggg gatccagaca tgataagata
     3961 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     4021 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     4081 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttcgga
     4141 tcctctagag tcgacctgca ggcatgcaag cttggcgtaa tcatggtcat agctgtttcc
     4201 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     4261 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     4321 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     4381 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     4441 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     4501 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     4561 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4621 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4681 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4741 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     4801 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4861 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     4921 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     4981 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     5041 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     5101 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     5161 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     5221 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     5281 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     5341 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     5401 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     5461 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     5521 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     5581 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     5641 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     5701 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     5761 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     5821 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     5881 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     5941 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     6001 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     6061 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     6121 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     6181 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     6241 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     6301 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     6361 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     6421 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     6481 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     6541 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     6601 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
     6661 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     6721 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     6781 acgttgtaaa acgacggcca gt
//
