LOCUS       sequence-211475-       13357 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of BE3-P2A-GFP (not 
            codon optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13357)
  AUTHORS   Zafra MP, Schatoff EM, Katti A, Foronda M, Breinig M, Schweitzer AY,
            Simon A, Han T, Goswami S, Montgomery E, Thibado J, Kastenhuber ER, 
            Sanchez-Rivera FJ, Shi J, Vakoc CR, Lowe SW, Tschaharganeh DF, Dow 
            LE
  TITLE     Optimized base editors enable efficient editing in cells, organoids 
            and mice.
  JOURNAL   Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
  PUBMED    29969439
REFERENCE   2  (bases 1 to 13357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194."
COMMENT     Sequence Label: pLenti-BE3-P2A-GFP-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..13357
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..252
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             253..433
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    480..605
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1098..1331
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1516..1560
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1744..1861
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1930..2141
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2167..2853
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             join(2902..6300,6301..6303,6304..6900,6901,6902..6904,
                     6905..6906,6907..6909,6910..7002)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(2902..2925,2926..2928,2929..7002)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7015..7263
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7276..7296
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7312..7368
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(7369..7371,7372..7374,7375..8088)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7414..7435)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7675..7694)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8022..8043
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        8100..8599
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8135..8158)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8532..8551
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8620..9219
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8620..8639)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9116..9136
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9235..9823
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9288..9308)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9706..9717)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9895..10128
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10206..10327
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10243..10262)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10297..10316
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10367..10502
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10522..10541)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10523..10541)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10551..10568)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10551..10567)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10560..10582)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10709..11164
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10796..10815)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11006..11027
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11190..11294
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11295..11363,11364..12155)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11513..11532)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12326..12914
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12815..12834
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13068..13085
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13202..13223
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13238..13243,13244..13261,13262..13268)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13276..13292
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13281..13303
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13300..13316
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13300..13316
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13335..13355
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13337..13355
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
       61 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
      121 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
      181 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
      241 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
      301 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
      361 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
      421 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
      481 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
      541 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
      601 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
      661 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
      721 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
      781 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
      841 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
      901 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
      961 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     1021 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     1081 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     1141 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     1201 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     1261 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     1321 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     1381 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     1441 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     1501 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     1561 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     1621 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     1681 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccagacca
     1741 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1801 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1861 tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagt ggctccggtg
     1921 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     1981 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     2041 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     2101 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtgtcgtga cgcgggatcc
     2161 gccaccatga gctcagagac tggcccagtg gctgtggacc ccacattgag acggcggatc
     2221 gagccccatg agtttgaggt attcttcgat ccgagagagc tccgcaagga gacctgcctg
     2281 ctttacgaaa ttaattgggg gggccggcac tccatttggc gacatacatc acagaacact
     2341 aacaagcacg tcgaagtcaa cttcatcgag aagttcacga cagaaagata tttctgtccg
     2401 aacacaaggt gcagcattac ctggtttctc agctggagcc catgcggcga atgtagtagg
     2461 gccatcactg aattcctgtc aaggtatccc cacgtcactc tgtttattta catcgcaagg
     2521 ctgtaccacc acgctgaccc ccgcaatcga caaggcctgc gggatttgat ctcttcaggt
     2581 gtgactatcc aaattatgac tgagcaggag tcaggatact gctggagaaa ctttgtgaat
     2641 tatagcccga gtaatgaagc ccactggcct aggtatcccc atctgtgggt acgactgtac
     2701 gttcttgaac tgtactgcat catactgggc ctgcctcctt gtctcaacat tctgagaagg
     2761 aagcagccac agctgacatt ctttaccatc gctcttcagt cttgtcatta ccagcgactg
     2821 cccccacaca ttctctgggc caccgggttg aaaagcggca gcgagactcc cgggacctca
     2881 gagtccgcca cacccgaaag tgataaaaag tattctattg gtttagccat cggcactaat
     2941 tccgttggat gggctgtcat aaccgatgaa tacaaagtac cttcaaagaa atttaaggtg
     3001 ttggggaaca cagaccgtca ttcgattaaa aagaatctta tcggtgccct cctattcgat
     3061 agtggcgaaa cggcagaggc gactcgcctg aaacgaaccg ctcggagaag gtatacacgt
     3121 cgcaagaacc gaatatgtta cttacaagaa atttttagca atgagatggc caaagttgac
     3181 gattctttct ttcaccgttt ggaagagtcc ttccttgtcg aagaggacaa gaaacatgaa
     3241 cggcacccca tctttggaaa catagtagat gaggtggcat atcatgaaaa gtacccaacg
     3301 atttatcacc tcagaaaaaa gctagttgac tcaactgata aagcggacct gaggttaatc
     3361 tacttggctc ttgcccatat gataaagttc cgtgggcact ttctcattga gggtgatcta
     3421 aatccggaca actcggatgt cgacaaactg ttcatccagt tagtacaaac ctataatcag
     3481 ttgtttgaag agaaccctat aaatgcaagt ggcgtggatg cgaaggctat tcttagcgcc
     3541 cgcctctcta aatcccgacg gctagaaaac ctgatcgcac aattacccgg agagaagaaa
     3601 aatgggttgt tcggtaacct tatagcgctc tcactaggcc tgacaccaaa ttttaagtcg
     3661 aacttcgact tagctgaaga tgccaaattg cagcttagta aggacacgta cgatgacgat
     3721 ctcgacaatc tactggcaca aattggagat cagtatgcgg acttattttt ggctgccaaa
     3781 aaccttagcg atgcaatcct cctatctgac atactgagag ttaatactga gattaccaag
     3841 gcgccgttat ccgcttcaat gatcaaaagg tacgatgaac atcaccaaga cttgacactt
     3901 ctcaaggccc tagtccgtca gcaactgcct gagaaatata aggaaatatt ctttgatcag
     3961 tcgaaaaacg ggtacgcagg ttatattgac ggcggagcga gtcaagagga attctacaag
     4021 tttatcaaac ccatattaga gaagatggat gggacggaag agttgcttgt aaaactcaat
     4081 cgcgaagatc tactgcgaaa gcagcggact ttcgacaacg gtagcattcc acatcaaatc
     4141 cacttaggcg aattgcatgc tatacttaga aggcaggagg atttttatcc gttcctcaaa
     4201 gacaatcgtg aaaagattga gaaaatccta acctttcgca taccttacta tgtgggaccc
     4261 ctggcccgag ggaactctcg gttcgcatgg atgacaagaa agtccgaaga aacgattact
     4321 ccatggaatt ttgaggaagt tgtcgataaa ggtgcgtcag ctcaatcgtt catcgagagg
     4381 atgaccaact ttgacaagaa tttaccgaac gaaaaagtat tgcctaagca cagtttactt
     4441 tacgagtatt tcacagtgta caatgaactc acgaaagtta agtatgtcac tgagggcatg
     4501 cgtaaacccg cctttctaag cggagaacag aagaaagcaa tagtagatct gttattcaag
     4561 accaaccgca aagtgacagt taagcaattg aaagaggact actttaagaa aattgaatgc
     4621 ttcgattctg tcgagatctc cggggtagaa gatcgattta atgcgtcact tggtacgtat
     4681 catgacctcc taaagataat taaagataag gacttcctgg ataacgaaga gaatgaagat
     4741 atcttagaag atatagtgtt gactcttacc ctctttgaag atcgggaaat gattgaggaa
     4801 agactaaaaa catacgctca cctgttcgac gataaggtta tgaaacagtt aaagaggcgt
     4861 cgctatacgg gctggggacg attgtcgcgg aaacttatca acgggataag agacaagcaa
     4921 agtggtaaaa ctattctcga ttttctaaag agcgacggct tcgccaatag gaactttatg
     4981 cagctgatcc atgatgactc tttaaccttc aaagaggata tacaaaaggc acaggtttcc
     5041 ggacaagggg actcattgca cgaacatatt gcgaatcttg ctggttcgcc agccatcaaa
     5101 aagggcatac tccagacagt caaagtagtg gatgagctag ttaaggtcat gggacgtcac
     5161 aaaccggaaa acattgtaat cgagatggca cgcgaaaatc aaacgactca gaaggggcaa
     5221 aaaaacagtc gagagcggat gaagagaata gaagagggta ttaaagaact gggcagccag
     5281 atcttaaagg agcatcctgt ggaaaatacc caattgcaga acgagaaact ttacctctat
     5341 tacctacaaa atggaaggga catgtatgtt gatcaggaac tggacataaa ccgtttatct
     5401 gattacgacg tcgatcacat tgtaccccaa tcctttttga aggacgattc aatcgacaat
     5461 aaagtgctta cacgctcgga taagaaccga gggaaaagtg acaatgttcc aagcgaggaa
     5521 gtcgtaaaga aaatgaagaa ctattggcgg cagctcctaa atgcgaaact gataacgcaa
     5581 agaaagttcg ataacttaac taaagctgag aggggtggct tgtctgaact tgacaaggcc
     5641 ggatttatta aacgtcagct cgtggaaacc cgccaaatca caaagcatgt tgcacagata
     5701 ctagattccc gaatgaatac gaaatacgac gagaacgata agctgattcg ggaagtcaaa
     5761 gtaatcactt taaagtcaaa attggtgtcg gacttcagaa aggattttca attctataaa
     5821 gttagggaga taaataacta ccaccatgcg cacgacgctt atcttaatgc cgtcgtaggg
     5881 accgcactca ttaagaaata cccgaagcta gaaagtgagt ttgtgtatgg tgattacaaa
     5941 gtttatgacg tccgtaagat gatcgcgaaa agcgaacagg agataggcaa ggctacagcc
     6001 aaatacttct tttattctaa cattatgaat ttctttaaga cggaaatcac tctggcaaac
     6061 ggagagatac gcaaacgacc tttaattgaa accaatgggg agacaggtga aatcgtatgg
     6121 gataagggcc gggacttcgc gacggtgaga aaagttttgt ccatgcccca agtcaacata
     6181 gtaaagaaaa ctgaggtgca gaccggaggg ttttcaaagg aatcgattct tccaaaaagg
     6241 aatagtgata agctcatcgc tcgtaaaaag gactgggacc cgaaaaagta cggtggcttc
     6301 gatagcccta cagttgccta ttctgtccta gtagtggcaa aagttgagaa gggaaaatcc
     6361 aagaaactga agtcagtcaa agaattattg gggataacga ttatggagcg ctcgtctttt
     6421 gaaaagaacc ccatcgactt ccttgaggcg aaaggttaca aggaagtaaa aaaggatctc
     6481 ataattaaac taccaaagta tagtctgttt gagttagaaa atggccgaaa acggatgttg
     6541 gctagcgccg gagagcttca aaaggggaac gaactcgcac taccgtctaa atacgtgaat
     6601 ttcctgtatt tagcgtccca ttacgagaag ttgaaaggtt cacctgaaga taacgaacag
     6661 aagcaacttt ttgttgagca gcacaaacat tatctcgacg aaatcataga gcaaatttcg
     6721 gaattcagta agagagtcat cctagctgat gccaatctgg acaaagtatt aagcgcatac
     6781 aacaagcaca gggataaacc catacgtgag caggcggaaa atattatcca tttgtttact
     6841 cttaccaacc tcggcgctcc agccgcattc aagtattttg acacaacgat agatcgcaaa
     6901 cgatacactt ctaccaagga ggtgctagac gcgacactga ttcaccaatc catcacggga
     6961 ttatatgaaa ctcggataga tttgtcacag cttgggggtg actctggtgg ttctactaat
     7021 ctgtcagata ttattgaaaa ggagaccggt aagcaactgg ttatccagga atccatcctc
     7081 atgctcccag aggaggtgga agaagtcatt gggaacaagc cggaaagcga tatactcgtg
     7141 cacaccgcct acgacgagag caccgacgag aatgtcatgc ttctgactag cgacgcccct
     7201 gaatacaagc cttgggctct ggtcatacag gatagcaacg gtgagaacaa gattaagatg
     7261 ctctctggtg gttctcccaa gaagaagagg aaagtcatgc atggcagcgg cgccaccaac
     7321 ttcagcctgc tgaagcaggc cggcgacgtg gaggagaacc ccggccccat ggtgagcaag
     7381 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     7441 ggccacaagt tcagcgtgtc tggcgagggc gagggcgatg ccacctacgg caagctgacc
     7501 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     7561 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     7621 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     7681 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     7741 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     7801 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggcg
     7861 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     7921 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     7981 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     8041 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagc tagcctaccg
     8101 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     8161 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     8221 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     8281 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     8341 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     8401 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     8461 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     8521 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     8581 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg
     8641 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     8701 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     8761 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     8821 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     8881 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     8941 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     9001 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     9061 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     9121 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     9181 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgat tctagaaagt cgacaatcaa
     9241 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     9301 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     9361 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     9421 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     9481 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     9541 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     9601 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     9661 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     9721 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     9781 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     9841 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     9901 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     9961 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
    10021 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
    10081 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
    10141 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
    10201 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
    10261 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
    10321 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
    10381 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
    10441 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
    10501 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
    10561 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
    10621 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
    10681 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
    10741 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
    10801 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
    10861 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
    10921 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
    10981 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
    11041 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
    11101 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
    11161 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
    11221 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
    11281 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
    11341 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
    11401 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
    11461 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
    11521 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
    11581 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
    11641 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
    11701 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
    11761 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
    11821 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
    11881 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    11941 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    12001 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    12061 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    12121 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    12181 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    12241 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    12301 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    12361 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    12421 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    12481 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    12541 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    12601 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    12661 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    12721 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    12781 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    12841 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    12901 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    12961 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    13021 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    13081 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    13141 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    13201 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    13261 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
    13321 attacgccaa gcgcgcaatt aaccctcact aaaggga
//
