LOCUS       sequence-211479-       11497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  puro-selectable lentivirus vector for inducible expression of a 
            UBAP2L-mCherry fusion protein lacking the UBA domain of UBAP2L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11497)
  AUTHORS   Markmiller S, Soltanieh S, Server KL, Mak R, Jin W, Fang MY, Luo EC,
            Krach F, Yang D, Sen A, Fulzele A, Wozniak JM, Gonzalez DJ, Kankel 
            MW, Gao FB, Bennett EJ, Lecuyer E, Yeo GW
  TITLE     Context-Dependent and Disease-Specific Diversity in Protein 
            Interactions within Stress Granules.
  JOURNAL   Cell. 2018 Jan 25;172(3):590-604.e13. doi: 
            10.1016/j.cell.2017.12.032.
  PUBMED    29373831
REFERENCE   2  (bases 1 to 11497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.12"; journalName: "Cell"; date: "2018-01-25- 
            25"; volume: "172"; issue: "3"; pages: "590-604.e13"
COMMENT     Sequence Label: pLIX403_deltaUBA_UBAP2L_mCherry
FEATURES             Location/Qualifiers
     source          1..11497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    547..571
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3604..4314
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3749..3767)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4017..4036
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(4316..4340)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4351..4371
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             4372..4395
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4412..4429
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        4439..4949
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4868..4886
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4959..5555
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4959..4978)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5455..5475
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5556..5609
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5610..6227,6228..6233,6234..6269,6270..6272,6273..6308,
                     6309..6311,6312..6347,6348..6356)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6396..6416)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6814..6825)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7030..7263
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7335..7456
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7372..7391)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7426..7445
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7496..7631
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7631..7702
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7703..7771,7772..8563)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7921..7940)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8734..9322
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9223..9242
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9476..9493
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9610..9631
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9646..9651,9652..9669,9670..9676)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9684..9700
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9689..9711
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9708..9724
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9708..9724
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9743..9763
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9745..9763
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9791..10017
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10018..10198
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10245..10370
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10863..11096
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11281..11325
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcatcaacaa gtttgtacaa aaaagcaggc ttcaccatga tgacatcggt gggcactaac
      601 cgagcccggg gaaactggga acaacctcaa aacaaggatg gtggccagac ggaatccaat
      661 gaggaaggca aagaaaatcg agaccgggac agagactata gtcggcgacg tggtgggcca
      721 ccaagacggg ggagaggtgc cagccgtgga cgagagtttc gaggtcagga aaatggattg
      781 gatggcacca agagtggagg gccttctgga agaggaacag aaagaggcag aaggggccgt
      841 ggccgaggca gaggtggctc tggtaggcga ggaggaaggt tttctgctca aggaatggga
      901 acctttaacc cagctgatta tgcagagcca gccaatactg atgataacta tggcaatagc
      961 agcggcaata cgtggaacaa cactggccac tttgaaccag atgatgggac gagtgcatgg
     1021 aggactgcaa cagaggagtg ggggactgaa gattggaatg aagatctttc tgagaccaag
     1081 atcttcactg cctctaatgt gtcttcagtg cctctgcctg cggagaatgt gacaatcact
     1141 gctggtcaga gaattgacct tgctgttctg ctggggaaga caccatctac aatggagaat
     1201 gattcatcta atctggatcc gtctcaggct ccttctctgg cccagcctct ggtgttcagt
     1261 aattcgaagc agactgccat atcacagcct gcttcaggga acacattttc tcatcacagt
     1321 atggtgagca tgttagggaa aggatttggt gatgtcggtg aagctaaagg cggcagtact
     1381 acaggctccc agttcttgga gcaattcaag actgcccaag ccctggctca gttggcagct
     1441 cagcattctc agtctggaag caccaccacc tcctcttggg acatgggctc gacgacacaa
     1501 tccccatcac tggtgcagta tgatttgaag aacccaagtg attcagcagt gcacagcccc
     1561 tttacaaagc gccaggcttt taccccatct tcaaccatga tggaggtgtt ccttcaggag
     1621 aagtcacctg cagtggctac ctccacagct gcacctccac ctccgtcttc tcctctgcca
     1681 agcaaatcca catcggctcc acagatgtcg cctggatctt cagacaacca gtcctctagc
     1741 cctcagccgg ctcaccagaa actgaaacag cagaagaaaa aagcctcctt gacttctaag
     1801 attcctgctc tggctgtgga gatgcctggc tcagcagata tctcagggct aaacctgcag
     1861 tttggggcat tgcagtttgg gtcagagcct gtcctttctg attatgagtc cacccccacc
     1921 acgagcgcct cttcaagcca ggctccaagt agcctgtata ccagcacggc cagtgaatca
     1981 tcctctacaa tttcatctaa ccagagtcag gagtctggtt atcagagcgg cccaattcag
     2041 tcgacaacct atacctccca aaataatgct cagggccctc tttatgaaca gagatccaca
     2101 cagactcggc ggtaccccag ctccatctct tcatcacccc aaaaggacct gactcaggca
     2161 aagaatggct tcagttctgt gcaggccacg cagttacaga ccacacaatc tgttgaaggt
     2221 gctacaggct ctgcagtgaa atctgattca ccttccactt ctagcatccc ccctctcaat
     2281 gaaacggtat ctgcagcttc cttactgacg acaaccaatc agcattcatc ctccttgggt
     2341 ggcttgagcc acagtgagga gattccaaat actaccacca cacaacacag cagcacgtta
     2401 tctacgcagc agaataccct ttcatcatca acatcttctg ggcgcacttc gacatccact
     2461 cttttgcaca caagtgtgga gagtgaggcg aatctccatt cttcctccag cactttttcc
     2521 accacatcca gcacagtctc tgcacctccc ccagtggtca gtgtctcctc cagtctcaat
     2581 agtggcagta gcctgggcct cagcctaggc agcaactcca ctgtcacagc ctcgactcga
     2641 agctcagttg ctacgacttc aggaaaagct cctcccaacc tccctcctgg ggtcccgccg
     2701 ttgttgccta atccgtatat tatggctcca gggctgttac atgcctaccc gccacaagta
     2761 tatggttatg atgacttgca gatgcttcag acaagatttc cattggatta ctacagcatc
     2821 ccatttccca cacccactac tccgctgact gggagggatg gtagcctggc cagcaaccct
     2881 tattctggtg acctcacaaa gttcggccgt ggggatgcct cctccccagc cccggccaca
     2941 accttggccc aaccccaaca gaaccagacg cagactcacc ataccacgca gcagacattc
     3001 ctgaacccgg cgctgcctcc tggctacagt tacaccagcc tgccatacta tacaggggtc
     3061 ccgggcctcc ccagcacctt ccagtatggg cctgctgtgt tccctgtggc tcctacctct
     3121 tccaagcagc atggtgtgaa tgtcagtgtg aatgcatcgg ccaccccttt ccaacagccg
     3181 agtggatatg ggtctcatgg atacaacact ggtgtttcag tcacctccag taacacgggc
     3241 gtgccagata tctcgggttc tgtgtactcc aaaacccagc agtcctttga gaaacaaggt
     3301 tttcattccg gtactcctgc tgcttccttc aacttgcctt cagccctagg aagtgggggc
     3361 cccatcaatc cggccacagc tgctgcctac ccacctgccc cctttatgca cattctgacc
     3421 ccccatcagc agccgcattc tcagatcctt caccatcacc tgcagcagga tggccagacg
     3481 ggcagcgggc aacgtagcca gaccagctcc atcccgcaga agccccagac caacaagtct
     3541 gcctacaaca gctacagctg gggggccaac accggtgtgg acaccggcaa cggttccgct
     3601 agcatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     3661 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     3721 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     3781 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     3841 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     3901 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     3961 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     4021 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     4081 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     4141 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     4201 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     4261 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctctacaa gtaaaaccca
     4321 gctttcttgt acaaagtggt tgcaacgcgt gaaaacctgt attttcagtc cgattacaag
     4381 gacgacgatg acaagtaggc atagaccggt ccaccaccac caccaccact aaggatccgg
     4441 ggttggggtt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg
     4501 ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg
     4561 ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg
     4621 ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg
     4681 aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc
     4741 gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg
     4801 gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc
     4861 ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg
     4921 ttgaccgaat caccgacctc tctccccagc aattcaccat gaccgagtac aagcccacgg
     4981 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     5041 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     5101 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     5161 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     5221 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     5281 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     5341 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     5401 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     5461 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     5521 cgcgcacctg gtgcatgacc cgcaagcccg gtgccgaagg tagaggttct ctcctcactt
     5581 gtggtgatgt tgaagaaaac cctggtccaa tgtctagact ggacaagagc aaagtcataa
     5641 acggagctct ggaattactc aatggtgtcg gtatcgaagg cctgacgaca aggaaactcg
     5701 ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc
     5761 tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccacttc tgccccctgg
     5821 aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcataccgc tgtgctctcc
     5881 tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg
     5941 aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac
     6001 tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc
     6061 atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga
     6121 gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg
     6181 aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccgaccgacg
     6241 cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg
     6301 atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtaactaa
     6361 gtaaggatcg atccaagata tcgtattctt aactatgttg ctccttttac gctatgtgga
     6421 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     6481 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     6541 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     6601 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     6661 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     6721 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     6781 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     6841 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     6901 agtcggatct ccctttgggc cgcctccccg cctgtttcgc ctcggcgtcc ggactagagg
     6961 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     7021 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     7081 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7141 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7201 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7261 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     7321 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7381 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7441 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     7501 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7561 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     7621 ttttggaggc ctttcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     7681 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     7741 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     7801 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     7861 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     7921 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     7981 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     8041 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     8101 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     8161 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     8221 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     8281 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     8341 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     8401 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     8461 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     8521 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     8581 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     8641 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     8701 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     8761 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     8821 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     8881 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     8941 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     9001 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     9061 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     9121 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     9181 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     9241 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     9301 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     9361 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     9421 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     9481 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     9541 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     9601 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     9661 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     9721 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
     9781 gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
     9841 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
     9901 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
     9961 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
    10021 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
    10081 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
    10141 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
    10201 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
    10261 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
    10321 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
    10381 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
    10441 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
    10501 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
    10561 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
    10621 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
    10681 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
    10741 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
    10801 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
    10861 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
    10921 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
    10981 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
    11041 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
    11101 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
    11161 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
    11221 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
    11281 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
    11341 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
    11401 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
    11461 ttatcgtttc agacccacct cccaaccccg aggggac
//
