LOCUS       sequence-211482-       17142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17142)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 17142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU33.2-2.2
FEATURES             Location/Qualifiers
     source          1..17142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1169..1468
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        3495..3715
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             3716..4519
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     terminator      9860..10047
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(9915..9939)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(12768..12788)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12768..12786)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(13125..13713)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13205..13224)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13884..14675,14676..14744))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14507..14526
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14745..14849)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      14876..16218
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(15839..15886)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     16259..16277
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(16619..16635)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16619..16635)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 aatgttttca agtctcttgt tgctcaacct attgcctctg gccatcgcgg ctccagccaa
       61 gcgagctgag ccggcaccac tccttgtccc acgaggtgac actatccccg acaagtatat
      121 tgtcaagtac aaagagacat ttgatatgtc agctgccgac atcaccatca aggaatacca
      181 tgccaaggcg gagaaaacgt actctcacgt cttcaacgga ttcgctggcg cgttgaacgc
      241 gacgtccatt gagacgcttc gtaatcatcc tgctgttgat tttattgaaa acgatgccac
      301 tgtcaagatt agcgcatttg tcgaacagcc gggtgcccct tggggtctgt ctcgcatttc
      361 gcaccgacag cgcggagcaa gtagctacgc ctatgatgac agtgccggtg aaggcacatg
      421 cgcgtatgtt atcgatactg gagtcgaggc ttcgcatccg aaattcgaag gtcgagccga
      481 gtttactagg tcctttgttg ccggagagac ttctgatcgc aacggtcatg gcacccacgt
      541 ggccggaacc attggcagta aagactttgg cgtggctaag aaaaccaaaa tccgtggaat
      601 caaggttctg agtgaccaag gcagcggcga ttactccggc attcttgccg gtatggactt
      661 tgctatccaa gactctcgag aacgaccctg ccccaagggc gtcgtcgcca acatgagctt
      721 gggaggcggc tattcagctg ccatcaatca ggctgctgca aagatgatcc agtctggtgt
      781 attccttgct gttgcggcag gaaacgacgc gaaggatgcc tcccaaacgt cccctgcctc
      841 tgagcctagt gtatgcactg taggagcaac ggattcttct gatagacttt cctccttttc
      901 aaacttcggg cctgctgttg acattcttgc ccctggaagc gatatcctgt caacttggat
      961 aggtggcgct actaaatcta tctctggtac ttccatggcc acgccacata ttgttggtct
     1021 tggtgcctac ttggcttctt tggaaggctt ccccggtgca caggctcttt gcgaaagaat
     1081 tcgctccctg gctacaaaaa atactattag cggtgtccca ggaggaactg tcaatttgct
     1141 tgctttcaac gggaatccat ctggataagc ggatctctta tgtctttacg atttatagtt
     1201 ttcattatca agtatgccta tattagtata tagcatcttt agatgacagt gttcgaagtt
     1261 tcacgaataa aagataatat tctacttttt gctcccaccg cgtttgctag cacgagtgaa
     1321 caccatccct cgcctgtgag ttgtacccat tcctctaaac tgtagacatg gtagcttcag
     1381 cagtgttcgt tatgtacggc atcctccaac aaacagtcgg ttatagtttg tcctgctcct
     1441 ctgaatcgtc tccctcgata tttctcattt tccttcgcat gccagcattg aaatgatcga
     1501 agttcaatga tgaaacggta attcttctgt catttactca tctcatctca tcaagttata
     1561 taattctata cggatgtaat ttttcacttt tcgtcttgac gtccacccta taatttcaat
     1621 tattgaaccc tcacgatcca gttctccagt gacacagcct ttatctggtc aaacctttct
     1681 ttctaatcac ctatgctgat gcttaattaa gggatttttg tctccatcaa cggcatgcgc
     1741 ccaaaaatga cgtttttttt aacccataga cacgaaacta cccattttcc accggcctga
     1801 cctaccaccg gaacaacggc catctccaac ttgcaagttg gggaaattaa gagcatcgca
     1861 ggtttaatgg aagaaaaaaa aaaggtacag cacagcgcaa atggagttag ttcccttatg
     1921 tcacacactc acacacagtc ggtcagatca agcatactgg gtgcgtataa atagagtggc
     1981 cattgccacc ctgtttatct caaaatctgt cttgttagtg gtcttctccc tttttcaggt
     2041 tacaattctc ttgtttctac ttagtatata agtatatcaa gctatattaa gcatactatc
     2101 aactgtcaac tctatcctca aaatacaata caaaatgtgg cgcatcccaa cggacacccc
     2161 tcaaacttcc actcccaacg gacgcaccaa gctccaggaa gtcaccccag aaaccccctt
     2221 ggacgaaatc ttccaaaagt ggggagaaga tggcgccctt gtcatcaaag gcctcctcac
     2281 ccctggtcaa gtagccgaat tcacctctga aatcgaacaa gtcattgaaa aaactcggcg
     2341 tggcgctctg ctggaccacc cgctccttca agcctttcac ggccgccgaa cgaagcgcgc
     2401 cgccggtctc gccaaccata gcaaggtgtt tcgcgagaac cttctcgaga acgacctagt
     2461 tcatgccata tgccaccgca actacactac gggaggcaac gccggcgatt actggctgtc
     2521 cacggggtca actttgtttg ccggcggtcc gcaggacccg caggagctgc atcgagactt
     2581 gaacaactac cctcctgccg tcttgcttgg cccagacttt gacgatgtgc aaatgaactt
     2641 tctgattgcc ctcagcccgt tcagggagga caacggcgcg acccgggtca tcccagggag
     2701 tcagaagtgg ccctttgacc agcgcggcaa ccaggaaatg acgatcccgg ccatcatgga
     2761 cccgggcgac tgcctccttt tgagtggcaa ggtcgtgcac ggcatgggtg cgaatacaac
     2821 cacggaggag cgaaaggcat tgcagctgtc tgtttgcgca ggctatctga cgccatccga
     2881 ggcgcatccg agcattgtca ccctagagac tgccaagaag ctgtctccaa gggcgcagcg
     2941 atttttgggt ttccggtccc agtaccctag ggggtcacct ggattgtgga ccaaggatta
     3001 cactgagctg gctttgcatt tggaactgga tgatcttgat ggcctgatgg tttatcttcg
     3061 tgaccttgcg aaaagtcatc cagttgaaga cccggttggc aaattgtttt agacaaatcg
     3121 ctcttaaata tatacctaaa gaacattaaa gctatattat aagcaaagat acgtaaattt
     3181 tgcttatatt attatacaca tatcatattt ctatattttt aagatttggt tatataatgt
     3241 acgtaatgca aaggaaataa attttataca ttattgaaca gcgtccaagt aactacatta
     3301 tgtgcactaa tagtttagcg tcgtgaagac tttattgtgt cgcgaaaagt aaaaatttta
     3361 aaaattagag caccttgaac ttgcgaaaaa ggttctcatc aactgtttaa aaggaggata
     3421 tcaggtccta tttctgacaa acaatataca aatttagttt caaagatgaa tcagtgcgcg
     3481 aaggacataa ctcattcaat tcaattcatc attttttttt tattcttttt tttgatttcg
     3541 gtttccttga aatttttttg attcggtaat ctccgaacag aaggaagaac gaaggaagga
     3601 gcacagactt agattggtat atatacgcat atgtagtgtt gaagaaacat gaaattgccc
     3661 agtattctta acccaactgc acagaacaaa aacctgcagg aaacgaagat aaatcatgtc
     3721 gaaagctaca tataaggaac gtgctgctac tcatcctagt cctgttgctg ccaagctatt
     3781 taatatcatg cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa
     3841 ggaattactg gagttagttg aagcattagg tcccaaaatt tgtttactaa aaacacatgt
     3901 ggatatcttg actgattttt ccatggaggg cacagttaag ccgctaaagg cattatccgc
     3961 caagtacaat tttttactct tcgaagacag aaaatttgct gacattggta atacagtcaa
     4021 attgcagtac tctgcgggtg tatacagaat agcagaatgg gcagacatta cgaatgcaca
     4081 cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag gcggcagaag aagtaacaaa
     4141 ggaacctaga ggccttttga tgttagcaga attgtcatgc aagggctccc tatctactgg
     4201 agaatatact aagggtactg ttgacattgc gaagagcgac aaagattttg ttatcggctt
     4261 tattgctcaa agagacatgg gtggaagaga tgaaggttac gattggttga ttatgacacc
     4321 cggtgtgggt ttagatgaca agggagacgc attgggtcaa cagtatagaa ccgtggatga
     4381 tgtggtctct acaggatctg acattattat tgttggaaga ggactatttg caaagggaag
     4441 ggatgctaag gtagagggtg aacgttacag aaaagcaggc tgggaagcat atttgagaag
     4501 atgcggccag caaaactaaa aaactgtatt ataagtaaat gcatgtatac taaactcaca
     4561 aattagagct tcaatttaat tatatcagtt attaccctat gcggtgtgaa ataccgcaca
     4621 gatgcgtaag gagaaaatac cgcatcagga taggaaaaaa ccgagcttcc tttcatccgg
     4681 cgcggctgtg ttctacatat cactgaagct ccgggtattt taagttatac aagggaaaga
     4741 tgccggctag actagcaagt tttaggctgc ttaacattat ggataggcgg ataaagggcc
     4801 caaacaggat tgtaaagctt agacgcttct ggttggacaa tggtacgttt gtgtattaag
     4861 taaggcttgg ctggggatag caacattggg cagagtatag aagaccacaa aaaaaaggta
     4921 tataagggca gagaagtctt tgtaatgtgt gtaacttctc ttccatgtgt aatcagtatt
     4981 tctacttact tcttaaatat acagaagtaa gacagataac caacagcctt tcccagatat
     5041 acatatatat ctttatttca gcttaaacaa taattatatt tgtttaactc aaaaataaaa
     5101 aaaaaaaacc aaactcacgc aactaattat tccataataa aataacaaca tggccaaatc
     5161 ctatcatttg tccttggata ttgctaaagg catccctaag actctccttc tcaaccgaca
     5221 accctcaatc tctcacctga gagcaaaaga aggacgtgtg gatcactcga tgatgaagcg
     5281 acccgaggat tttcgcggaa aggcaagttt tctgctctgc ggggataccg tctttgttac
     5341 cggggggtct gggggattgg gcaaagccat atcccagcag ttagcagccc gaggggccca
     5401 tatcacaatt ttctcccgca ggccagacgc acttgccagg gccagagagg agatactttc
     5461 caagtgtttg actggggatc aggaggtcaa taccgtcgcc atcgacatgg cggatgcttc
     5521 aaaggttgac gaagcattta gaaaacagcc tcggattgct gacgtcttgt actgtgttgc
     5581 cggcggtaac catgctgaga acggatttct cgtagatatt aaggcacagg ctcttgaaag
     5641 ttgcatgcgg aacaactact ttaccgccgt gtacgcagcc aagtctctgc tcgacatatg
     5701 gacagaggat gatttgaaag gacccattca tccccgccct gaccccagga tacgtcagat
     5761 tgtctttgtc acaagcgccg ccgcgttttt gggttctccg ggctccattt cttacactcc
     5821 ggccaagtgt gcaacccgcg cctttgcgga tactctgcgg ttggaagtgc tgcggtactg
     5881 ctgccccgag tcgacctact cgatccactg cgcctttcca ggggactttg tatcccctgg
     5941 atttgtcttc gaacagaaga ccaagacgaa tctcacaaag cgcattcagg gtctcgacgg
     6001 ctacaccatg tcggagctcg aagctcggtt cccatcttcg gacaagatcg cttcactcat
     6061 cacttccgcc gtggacaggg gcgatttcat catctgtgac ggctcgctgg ctggctcatt
     6121 gctcttcacc aacatgattg ggccgtcacc aaagagggga ttgggcattg tggattcttt
     6181 gctttctgta tttaccgggt gtcttctctg gccatatctc aggtggaaat gggagtccat
     6241 gacccgcaag gacggcgaag agcacagaag agctaggtga agtgctttta actaagaatt
     6301 attagtcttt tctgcttatt ttttcatcat agtttagaac actttatatt aacgaatagt
     6361 ttatgaatct atttaggttt aaaaattgat acagttttat aagttacttt ttcaaagact
     6421 cgtgctgtct attgcataat gcactggaag gagaaaaaaa aggtgcacac gcgtggcttt
     6481 ttcttgaatt tgcagtttga aaaataacta catggatgat aagaaaacat ggagtacagt
     6541 cactttgaga accttcaatc agctggtaac gtcttcgtta attggatact caaaaaagat
     6601 ggatagcatg aatcacaaga tggaaggaaa tgcgggccac gaccacagtg atatgcatat
     6661 gggagatgga gatgatacct ccattgggcc gatgaagtta gtcgacggat agaagcggtt
     6721 gtcccctttc ccggcgagcc ggcagtcggg ccgaggttcg gataaatttt gtattgtgtt
     6781 ttgattctgt catgagtatt acttatgttc tctttaggta accccaggtt aatcaatcac
     6841 agtttcatac cggctagtat tcaaattatg acttttcttc tgcagtgtca gccttacgac
     6901 gattatctat gagctttgaa tatagtttgc cgtgattcgt atctttaatt ggataataaa
     6961 atgcgaagga tcgatgaccc ttattattat ttttctacac tggctaccga tttaactcat
     7021 cttcttgaaa gtatataagt aacagtaaaa tataccgtac ttctgctaat gttatttgtc
     7081 ccttattttt cttttcttgt cttatgctat agtacctaag aataacgact attgttttga
     7141 actaaacaaa gtagtaaaag cacataaaag aattaagaaa atggatttcc tggcggattt
     7201 tatcaaggct cagaagatca aggggcccca gatgaaagat gccccaacct tctaccgcaa
     7261 cctcgaggag gctttggacc tccggcgatc cgatcatggc atgttcacca acggtatgaa
     7321 gagtccctgg aagtcaggca aggccgtcga cttttgctcc aacgatcttc tgtccctggg
     7381 aaagacggga ctgcttcgca aggagttcct caacgagctg ggccgtaact ccgacttccc
     7441 catctatgca ggaggttcgc gcctggtgga cgggaactac gattacattg aacaagttga
     7501 gcaggaaatt gccgccttcc acggcgctga gatgtcactc attgtcggct ccggtttcga
     7561 ggctaacaat gccatctact cggcaatccc acgccccggc gacgccatag tctacgacga
     7621 gctcgtccac gccagcgtcc atgacggcat gacgcactcg ctggccttga ccaaggttgc
     7681 cttccgccat aatgatgtcg acgccttgag agatgcgcta gtagaacttg tcgactcaca
     7741 gcccttgatc aaggacggct cgcgcacggt tcttatatcc gtcgagtccg tgtacagcat
     7801 ggatggtgac gtttgccctc tacgcgagat gcttgagatt tcccgcgaag tgtgccccaa
     7861 gatgaatttt gcttttattg tcgacgaagc ccatgctacg gggataattg gccccaaggg
     7921 cgtaggactt gtcagtctct taggtctcga gaaggagatt gctattcgac tccatacata
     7981 tggaaaggct ttggcctcga caggagccgc cattttgagc aacggaacgg ttcgaacggc
     8041 tctgctcaat tttgcccgct ccgtcatcta caccactggc ccttcctttc cttcggttgc
     8101 atgtattcga gcagcctaca acttgatgcg caacggagaa acgacaaagt tacaaaacag
     8161 catccagcat ctcgtgaagc actttttcga aaccatcacg tcgaaccctg tgtgggaaaa
     8221 agccagcgag atggggattc tggccatccc tctggtcgac gattgggaag gacgcgagtt
     8281 ccagacgcac attgtacccc tctggacacg gcagcggtat aactggtggc tcgtattcca
     8341 tctgcagttg gcgggaattg cggccgtccc cattgactac cccacggttc caaagggcca
     8401 gagcaggatt cggatcatgg ttcatagcgc caacacggag gccgaggtcg attttcttgc
     8461 cagcaccata tgtgaatggg cacaagagat gattggcatt gaggagtcgg gggaggtcaa
     8521 gggaaagacg cccaaagccg ctcagaaaat ctacgccttg atggctgctg ctagcgcttg
     8581 aatttaactc cttaagttac tttaatgatt tagtttttat tattaataat tcatgctcat
     8641 gacatctcat atacacgttt ataaaactta aatagattga aaatgtatta aagattcctc
     8701 agggattcga tttttttgga agtttttgtt tttttttcct tgagatgctg tagtatttgg
     8761 gaacaattat acaatcgaaa gatatatgct tacattcgac cgttttagcc gtgatcatta
     8821 tcctatagta acataacctg aagcataact gacactacta tcatcaatac ttgtcacatg
     8881 agaactctgt gaataattag gccactgaaa tttgatgcct gaaggaccgg catcacggat
     8941 tttcgataaa gcacttagta tcacactaat tggcttttcg ctagtcttat ctaaaaattg
     9001 cctttatagt ccgtctctcc agtcacggcc tgtgtaactg attaatcctg cctttctaat
     9061 caccattcta ctgtttaatt aagggatttt gtcttcatca acggcttccg cccaaaaaaa
     9121 agtatgacgt tttgcccgca ggcgtgaagc tgcccatctt cacgggcctg acctcctctg
     9181 ccggaacacc ggccatctcc aactcataaa ttggagaaat aagagaattt cagattttca
     9241 gaggatgaaa aaaaaaaggt agagagcata aaaatggggt tcactttttg gcaaagttac
     9301 agtatgctta ttacatataa atagagtgcc gataatggct ttttttcatc ttcgaaatac
     9361 gcttgctact gctcttccag cgtttttatt acttctttct tgtttctcct tagtatataa
     9421 aatatcaagc tacaacaagc atacaatcaa ctgtcaactg tcaattatat tataatacac
     9481 tatgctcggc tttgccaaaa cccttcttct tgctacccag ctggcatatt ttgccagtgc
     9541 gtcgcccacc ctcaacaata acaataagct tacgagcaaa tggcccgtgt gtatccctcc
     9601 cccaacacaa gtctcaacca agcgaacgcc gactgagaag cgagacgcca gcgttacgac
     9661 tccgttgccc acccagccag gcatggttgg caactgcaac aagttctgct ggatagagaa
     9721 tggcgacatc tgcgggcaga ttgctgagag caatgacatt tccactaagc aattcatcca
     9781 atggaacaaa ggcgctggtc cggattgcct cactctctgg gcgaaaacat atggttgcgt
     9841 gggcgttacg gactcgtaag cgaatttctt atgatttatg atttttatta ttaaataagt
     9901 tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt
     9961 cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat agcatgaggt
    10021 cgctcttatt gaccacacct ctaccggcat gcctctcaaa tcttttagcg ccaaggactc
    10081 caactaattg tatcttgaat ttgcctttac gatccgtttg tccagtcacg gcatgtatat
    10141 cttattaatc ctgcctttct aatcacgtat tctaatgttc aattaaggga ttttatcttc
    10201 atcaacggct cccacgcaaa aaatgacgtt ttgcacacag acacgaaata caccttccac
    10261 cggaacaacg gccatctcca acttataagt tggggaaata agacaatttc agacttcaga
    10321 gaatgaaaaa aaaaaaaggt acatcacaga tggggttcag gtttgctaca attgcaggga
    10381 gcctgtcaca tataaataga cctccagtga tgatatcttt cagtcttcaa acgtctcttg
    10441 tcacagttct ggtcgttcta tatcacatct ctcttggttc tacttattgt ctataatatc
    10501 aagctacagc aagcatacaa tcaactatct accataccat aatacacaat ggatcaacaa
    10561 ccagaaccgg accacggaca aaatatcagg cgaaggcgta gtcggcagtc gtctgcggcg
    10621 ccgtccgtcc agctagaagc gaatgcagct cctgctttac ctcaacgggc aggacggccg
    10681 ctttcctttt ggctcgcctt ttccggtcta tgtatcaaca cgtttctctt cgctctcgac
    10741 tcgtccactc tctccgtggc tcttccagcc attgcgcacg agttgcatgg cacaactttc
    10801 gagtcgttct gggccggaat cgcgtacctt ctttgcgtgg tcatcaccca gccattgtac
    10861 acctcactgt ccgacgtctt cggtcgcaag cctctgctct atgccgctct cctgctcttt
    10921 gccatcggct cgctagtctt tggtcttgcc aagaacatga cgtatatcat tgtcggccga
    10981 gcgtttcagg gctttggggg cgggggtatg gatctgctaa gcgagatcat cataacggat
    11041 ataacgacgc tgcaggagcg tgccatgttt ctcgggctgc tggctattcc catcgccatt
    11101 gggaccatca tcggtccaac tatcggcgcc gtcttcagcg aattcatctc ttggcgatgg
    11161 atcggctacg caaacttgcc ctttgtcgga gtctcgttcc ccatcacatt ctttttcctt
    11221 cgtctccgta cggtcaacga ctccctcatg gacgatgtga agaggctgga ttggggtggc
    11281 atggctctct ccttcaccgg aatgattctt ttcgtgctac ctcttagttg gggcggcgcc
    11341 ttgtactcgt gggcctcatg gcagacactt gtcccgttga ttatgggaat ccttattctg
    11401 gttggttact ttgcctacga gtcactaccc gccgctccga tcatgcccta tcgattgcta
    11461 cgatctacaa cagccaaggc gacactgctg gggaacttta tgcaaggctt cgttctcttc
    11521 ccgctgcaac agtacttgtt gctcttgttc caggcagtct acctactgag ccaaatcgac
    11581 acggctgtgg agctgctccc taccagcatc acatgcgtct tggccgccag tggtgtggtc
    11641 atcaccatag gtctactcaa atctggatat ctatggagta tcagactgtt ctgggttgtc
    11701 ctcacagtgg gcaccggcct tgtcgccctg ttagatgaag gctccccccg tgctatgcga
    11761 atggccatcc ccatcatctg gggcgcgggt ataggcgcat tgttgcgtct cctccacctt
    11821 ccaatgcaag caagtgtcga aaacgtggat gacacggggc tggccgtcgg gctcgtcctt
    11881 tggtttcgcc tggtgggagg cctcctcgga ctcgccgtct catcctcaat atttatcagt
    11941 gtctttgcca agtccattgc cggcgttgac aacctccccg agtctcttgc tcccctgaga
    12001 gaagcgagcc gggccattag ctttatacca acgctgaggg atctgggtct ttcccgggac
    12061 gtgttgggcc tagtcttggg agcttaccac aaagctttcc agacagtatt ttacgcaatg
    12121 actgcctttg gcggtgttgg tttcgtaagc tcactattta ccaaggagct cactctgcag
    12181 aaaagcgacc ttggccggca ggcgtttgag ggacagcaat tattacccta aggagattga
    12241 taagactttt ctagttgcat atcttttata tttaaatctt atctattagt taattttttg
    12301 taatttatcc ttatatatag tctggttatt ctaaaatatc atttcagtat ctaaaaattc
    12361 ccctcttttt tcagttatat cttaacaggc gacagtccaa atgttgattt atcccagtcc
    12421 gattcatcag ggttgtgaag cattttgtca atggtcgaaa tcacatcagt aatagtgcct
    12481 cttacttgcc tcatagaatt tctttctctt aacgtcaccg tttggtcttt tatagtttcg
    12541 aaatctatgg tgataccaaa tggtgttccc aattcatcgt tacgggcgta ttttttacca
    12601 attgaagtat tggaatcgtc aattttaaag tatatctctc ttttacgtaa agcctgcgag
    12661 atcctcttaa gtatagcggg gaagccatcg ttattcgata ttgtcgtaac aaatactttg
    12721 atcggcgcta tgcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg
    12781 ttaattgcgc gcttggcgag actcaaacaa gatatatttt gcccgcctta cagacgaaac
    12841 tagttggagg ttatggagca tactatcacg tgggcggcca ctggtgagtt actacacccc
    12901 aggggcaacg ttgatgctcc taaaaaactc tggctggacg caagccgtaa cacccgtgtc
    12961 acttcataat cgtttgcaat tcagggcttg atctacacat taccctgtta tccctattaa
    13021 ttaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    13081 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    13141 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    13201 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    13261 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    13321 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    13381 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    13441 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    13501 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    13561 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    13621 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    13681 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    13741 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    13801 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    13861 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    13921 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    13981 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    14041 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    14101 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    14161 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    14221 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    14281 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    14341 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    14401 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    14461 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    14521 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    14581 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    14641 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    14701 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    14761 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    14821 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgaacga
    14881 agcatctgtg cttcattttg tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac
    14941 aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc tattttacca
    15001 acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc gctaattttt
    15061 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt
    15121 taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat
    15181 ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc
    15241 tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct
    15301 attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa
    15361 gctgcgggtg cattttttca agataaaggc atccccgatt atattctata ccgatgtgga
    15421 ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat
    15481 tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc
    15541 gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta
    15601 atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga
    15661 aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt
    15721 ttgagcaatg tttgtggaag cggtattcgc aatattttag tagctcgtta cagtccggtg
    15781 cgtttttggt tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa agcgctctga
    15841 agttcctata ctttctagag aataggaact tcggaatagg aacttcaaag cgtttccgaa
    15901 aacgagcgct tccgaaaatg caacgcgagc tgcgcacata cagctcactg ttcacgtcgc
    15961 acctatatct gcgtgttgcc tgtatatata tatacatgag aagaacggca tagtgcgtgt
    16021 ttatgcttaa atgcgtactt atatgcgtct atttatgtag gatgaaaggt agtctagtac
    16081 ctcctgtgat attatcccat tccatgcggg gtatcgtatg cttccttcag cactaccctt
    16141 tagctgttct atatgctgcc actcctcaat tggattagtc tcatccttca atgctatcat
    16201 ttcctttgat attggatcat actaagaaac cattattatc atgacattaa cctataaaaa
    16261 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg agcgcgtttc
    16321 ggtgatgacg gtgaaaacct cggcgcgcca ttaccctgtt atccctaata tacaacgcaa
    16381 agaagctgga aaattggcga gggaatcctg tttctgtcta tccaagaatg ggcatgaggt
    16441 ggcaaccgtc gtgctagcgt acagggtgca ctttgtaacc atttgggaca ccggacactc
    16501 gctgttttcg aaattaccct ttaagcgcgg gtattgaacc aggcttatgc ccaagatcgt
    16561 agcaagcatt taaatgacgg tatcgataag cttgatatcg aattcctgca gcccggggga
    16621 tccactagtt ctagattaat taatatctaa aaattgcctt atgatccgtc tctccggtta
    16681 cagcctgtgt aactgattaa tcctgccttt ctaatcacca ttctaatgtt ttaattaagg
    16741 gattttgtct tcattaacgg ctttcgctca taaaaatgtt atgacgtttt gcccgcaggc
    16801 gggaaaccat ccacttcacg agactgatct cctctgccgg aacaccgggc atctccaact
    16861 tataagttgg agaaataaga gaatttcaga ttgagagaat gaaaaaaaaa aaaaaaaaaa
    16921 aggcagagga gagcatagaa atggggttca ctttttggta aagctatagc atgcctatca
    16981 catataaata gagtgccagt agcgactttt ttcacactcg aaatactctt actactgctc
    17041 tcttgttgtt tttatcactt cttgtttctt cttggtaaat agaatatcaa gctacaaaaa
    17101 gcatacaatc aactatcaac tattaactat atcgtaatac ac
//
