LOCUS       sequence-211485-       13141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13141)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 13141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU70.1-2.2
FEATURES             Location/Qualifiers
     source          1..13141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             7..30
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(7068..7088)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7068..7086)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(7425..8013)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7505..7524)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8184..8975,8976..9044))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8807..8826
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9045..9149)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9176..10518
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10139..10186)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     10559..10577
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10872..11279
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             11280..12374
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(12621..12637)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12621..12637)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 acaatggatt ataaggatga tgatgataag actagtatgg atgattcagt ccagccaaac
       61 ccctccatgc ctcctatact agtagggtcc acgcaagctt cgtgccaact attcctcttt
      121 ggggatctaa ccacatcttt cgagcaagac cttcgtcgac ttcttcatgt caaagataat
      181 gctaccttac attccttctt cgagaaagtc agtgctgcct ttcgtacgga atttgggaaa
      241 ctccctgctc gtcagcaagc cttgcttcct cgctttacca ccattaccga tcttcttgca
      301 aatttgggtg agacagaagg aaagccagcc ttgagtttcg ctctccactg tctctgtgag
      361 attggtcaat tcattagata ttttggagaa ggatccagac cttatcctag cgatgccaac
      421 acctatcttg tggcactgtg cactggctcg tttgctgccg ctgcaatcag tacctctcga
      481 acactttcag agttgattcc cgcaggaatc gaagccgttc ttgcagcctt caggaccggt
      541 ctgtgctcgc tggagcttcg aaatgatata gagcggccga gcagtcgaat acctcgatct
      601 tggtccgcga ttgtgagtgc ttcagaagca caggccgctg aacttctgca gagtttcaac
      661 tcaaaaaaca ctctatctgg aagctccagg ctttttctaa gcgccgtttc gccaaacagt
      721 ataacaatca gtgggcctcc aagcattcta cacaactttc tggcttcagt ttctttgaac
      781 tcgtattgcc taccgaacga gtcgccgttc catgctcctt ccctgtttgg gtcttatgag
      841 gttaatgaga taattagcta cttccctgat gaattgcttg gttcatacaa gccgcgaatt
      901 cctctgttat ctgctgcttc cggcaaacct atcgtcgctg aggacttcaa gaccctgctg
      961 catacggttg tgtcagacac tctgtgcgag caggttcgat gggacaacat cctccggtcg
     1021 ctcgtggaag atgtcttcca gcagaagtcc ttcaaagaat gcacaatatc acccgtattg
     1081 tctaatgctg ctcacctcct ctcttcagca ataacgcgcg ccacaaaaat caatgtcaca
     1141 acgaccaatt tgttgaatct cggcgtctcg gactcccagc ctgccaggcc tactggtaaa
     1201 ttcgagcagt caaagatagc catcactggg ttttcaggtc gtttcccgga agctgcatcg
     1261 aatgaggaat tttgggagct gctaagtgca ggacgagacg tccatcgcac aattcctgaa
     1321 gatcgcttca actggaaaac tcacttcgat gcagcaggta aggtaaagaa cacaagcagg
     1381 gttcagtatg gatgcttcat caatgagcca ggattatttg atgctcgatt tttcaacatg
     1441 tcccccagag aatctcaaaa cacagatcca gctcagaggt tagccatcac gacaacatac
     1501 gaggctatag aaatggcagg attggtccct aacaggacac cttccacaca acaagaccgc
     1561 attggtgtct tttttggggt gactagtgat gattggcgag aggtcaatag cggccaaaac
     1621 atcgacacat attttatccc tggcggaaac cgtgcatttg tccccggacg cataagctac
     1681 ttctttagat tttctggtcc aagcctgagt atcgatacag catgttcctc aagcttcgct
     1741 gcaatccaaa cggcgtgtaa ctatttatgg agaggagaat gtgatactgc agttgccgga
     1801 ggaaccaatg tactcaccaa tcctgacaac tttgctggcc ttgatcgtgg acacttccta
     1861 tcgaccacag gaaactgtaa tgcctttgat gacggtgcta gcggctactg cagagcagac
     1921 gccgtggggt ccgtcattct caagaggctc gaagatgcgc aagcggacaa cgatccaatc
     1981 tttggggtca tcgatggagc ttttacaaat cattgtggtc aaacagaatc catcacaaga
     2041 cctcacgagg gcgaccaagc ttccgtcttt aaccggatca tgaggaattc caacgtcaat
     2101 ccactggatg ttagctacat agaaatgcat ggaactggaa cccaagcagg agatgctact
     2161 gagatgaatt cagttctatc agttttcgtg cctggtcgcg agcgaatgcc acaccacccg
     2221 ctatatcttg ggtccgcgaa agccaacgtt ggtcacgcag aggcagccag tggtgtttct
     2281 gctttgatca aggtcctgat gatgatgaag aacaaccaga taccaccgca ttgcggaatc
     2341 aaaactaaga tcaaccgtaa ctatcctctt gatctcaaag agcgcaatgt caacattgcg
     2401 ttgaaaactg tcccttggca tcgaactgat tgcgtcggtg ggaagcgcac atgttttctg
     2461 aacaatttca gcgccgctgg aggtaacaca gccgtgctcc tagaagacgc tccgttacct
     2521 caatcagaaa acacagcaat agagactcgc tcagtacatc ccgtcatagt gactgcaaag
     2581 agtgtacagt ctctgaaaca aaatatcgag tccttgatca cctttcttga gaagaacccg
     2641 agcacctcgc ttccatcatt atcatacact actacggcac gacgaattca ccacaattac
     2701 cgcaccatct gctcagggtc agacatcgaa tccattcggg acgctctcag gcagaaagcc
     2761 agcagttccg acatcaagcc tattccaaat gtaaccaagc ttccgaaggt ggccttcgtc
     2821 ttcacaggtc aaggctgtct gtatgctggt gttggcaaac agctattcga gactgtctcg
     2881 catttcagag ctgatattct tcggttcgac aacatagctc agcggcaagg tttcccaagc
     2941 tttttgcctg tggtcgatgg tagcgtcgcc aatgttggtg agctagagcc agtggtttcc
     3001 catttggcag tgacatgcgt gcaaatggcg ctttctcgcc tctggatctc ttggggtgtg
     3061 gttccgagtt tgacaactgg ccacagcctg ggagaatacg ccgccctcta tgcggctggg
     3121 gtcgtgacag ccagcgacgc cattcacctt gttggtacca gagcacagtt gctatcgaag
     3181 cattgcaata aaggaacaca tactatgctg gccatcaaag catctctcga ctccatcaaa
     3241 ccccgtctaa gcgcctccgt ctgcgaagtc gcttgtttca accagcctgc cagcaatgtg
     3301 atcagcggac ccagcgaaga aatcagccgc ttaatgaggg aatatcaatc agacggtttc
     3361 gaatgtatcg agctagacat accgttcgct tttcattcag cacaagttga gcctatcttg
     3421 gagctattcg agagggcagc caacggcgtc cagttccatg ccccttcaat tccctacatg
     3481 tcgcctttgc tgggccgcat tatctcagat ggtgatgtgt taggaccatc ataccttgct
     3541 cgagcatgcc gcaatgcagt aaacttccaa ggtgctcttc aggctgcgaa agcttcatct
     3601 gttgtcaacg agaagtcagt ctggttggaa attggatcgc atccagcttg ttctggcatg
     3661 atcaagggaa cgctgggcac acatgccttg acaataccgt ctcttaaaaa agctacagac
     3721 acgtggaaag tgctagccag cgggctcgaa tcactttatc tcagcggcat tgagatcgat
     3781 tggaatgaat atcaccgaga ctttgagagc tcgcataagg ttctagagct tccaaggtac
     3841 tgttgggatt tgaagaatta ttggatccag tacaggaatg acttctgcct caccaaaggg
     3901 gacgatccac caccacaaaa agaaattggt gctgctcctg ctgttgagca gaagcctgtg
     3961 cctctctata tttcaccctc tgttcaacgt attcttgagg agcacaatgc agctgatgtg
     4021 tcaactcttc tcgccgaatc agacatacac gatccgcgac ttgctcccat cttccaaggc
     4081 cacaacgtga atggtacagc tctatgtcct tcatctctgt atgccgacat cgccctgacg
     4141 gtcgttgatt acatgctgaa gacaaacgat atgcaaaatg atactactgg ccttgacgtg
     4201 ggcgacgtga aggtcgacaa atcgcttata gctgattcta acgctgtgac acaactactt
     4261 cgtatttcgg cgtccgcaaa ttggtcaacc aaagtcgttt cacttgccct ctacagcatt
     4321 aacgcacagg gcagaaagtt agccgatcac gcgaaatgcc aggtccggat cactgaaaag
     4381 cagtcatggt ttcaagagtg gaagcgcagc tcgtatctca tcaagagcag agtcacatca
     4441 ctttacaaag gagtcgatgg aggcgattcg caccagatca agcgtggcat ggtctacaag
     4501 ctgttctctt ccattgtgga ctatgatgca agatatcaag ccatgcaaga ggtcgtactc
     4561 gatagcaacg agctcgaagc taccgcacag gtgtcattcc aggttgatga tgaaggattc
     4621 aacttcaacc cttgttggat tgacggcctt gggcatatcg ctgggttcat catgaatgga
     4681 aatgacaatg ttcactcgaa ggaccaggtg tttatcaatc atggctggga taccatgcga
     4741 tgcgcgacga agttcaggaa gggcaagact taccgaagct acaacaaaat gcagcttgtg
     4801 agtggatcat tatacgcggg cgatacgtat attcttgagg acgacaacgt tgttgctgtc
     4861 attgaaggtg tcaagttcgc tggaataccg cgccaagtcc tgaaccaggt attgcctcca
     4921 aagtctggag cagcaggacc cgcagtgact aaatcgcgtt cttcaacgca atcaaaaccg
     4981 gtgccgatgg ccacagccaa tccgcttcca aaatcaactc gcacagccac ggctactccg
     5041 ctccccaaac aagcaatatc accaccaaac tccaatacaa agcataccaa agcgtcaccg
     5101 acgaatagca ttgtgcccct agtattggca atcattgcag aggaggctgg cctcgacgcg
     5161 gccgagcttg gaccaagcag cgaatttgcc gattatggca ttgactcatt gttgtccctc
     5221 accataactg ggagaataca agaagagctc ggactcgatc taccctcttc actctttgtg
     5281 gaccagccta ctgtgaagga atttacagtg ttccttgata gtagcaacaa caggcccttg
     5341 ttgtcaagtc cttccggcaa ctcgacgcca gtctcaaatc cagagcaaga aaccctttcg
     5401 gaagctacgc cggaagacta cgaaacagac gcaacatcag ttacagacgg ccaaagcgtg
     5461 atggaaatca tccgcgccac gatcgctgca gagactggtg tctctcttga ggacttgaca
     5521 ccttcgacga gtttctcaga gcttggtgta gattctctgc tagcgctcac gatcatggga
     5581 aagcttggtg aagtcttaga aacggatctt ccgcaaacac ttcttgccga taacgacaat
     5641 ctgtccgaga tcgaaaaggc attgagcctg aagctgaagg gaccagacct caaccgaaat
     5701 cctgtggcta cttctccaag agcggacccg ttcgaccccg agatgcctcc tcacgccaca
     5761 tcagtcaagc tccaagggaa tcccaagaat gccacaaaga caatattcct ctttccagat
     5821 ggttctggat cagctacctc gtacgcttca attcctaaac tctcgcccga gatcgtggtg
     5881 tatggcttga actgtccctg gatgaagaca ccacaggata tgaaatgctc cttggaacac
     5941 ctgacaagca aatatattat cgagatccgc cgtcggcagc caaaaggccc ttattacttt
     6001 ggtggttggt cggcgggagg aatatgcgca tttgaagctg cgcaacagct ggccagccag
     6061 ggtgaagaga ctagacgact gatattgatt gattcaccaa atccagtcgg cttggagaat
     6121 ccgcctcagc gcatgtacga cttctttgaa agcttggact ttttcggcat gaatggcaag
     6181 gcggctccaa gctggctacg gccccatttc aatgccttca tcagcacgct ggatcattac
     6241 aaggtgaaac ctttttcagg tccacggctt gagactcacc taatctacgc tcgtgatgga
     6301 atttgcaaac atcccagtga tcctcgaccg gagattcgcc ccgatgatcc tcgggagatg
     6361 ctctggcttc tcaacaatcg cactgatttc agtggcaggg gctgggatga cttggttggg
     6421 aaggagaacc tgaagatcca agtgttggac gaggtcaatc atttttcgat ggtggcgcct
     6481 ggtccgaaaa ttcaagagct cggtgctttc atcgcacgtg caatggagtg aggagattga
     6541 taagactttt ctagttgcat atcttttata tttaaatctt atctattagt taattttttg
     6601 taatttatcc ttatatatag tctggttatt ctaaaatatc atttcagtat ctaaaaattc
     6661 ccctcttttt tcagttatat cttaacaggc gacagtccaa atgttgattt atcccagtcc
     6721 gattcatcag ggttgtgaag cattttgtca atggtcgaaa tcacatcagt aatagtgcct
     6781 cttacttgcc tcatagaatt tctttctctt aacgtcaccg tttggtcttt tatagtttcg
     6841 aaatctatgg tgataccaaa tggtgttccc aattcatcgt tacgggcgta ttttttacca
     6901 attgaagtat tggaatcgtc aattttaaag tatatctctc ttttacgtaa agcctgcgag
     6961 atcctcttaa gtatagcggg gaagccatcg ttattcgata ttgtcgtaac aaatactttg
     7021 atcggcgcta tgcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg
     7081 ttaattgcgc gcttggcgag actcaaacaa gatatatttt gcccgcctta cagacgaaac
     7141 tagttggagg ttatggagca tactatcacg tgggcggcca ctggtgagtt actacacccc
     7201 aggggcaacg ttgatgctcc taaaaaactc tggctggacg caagccgtaa cacccgtgtc
     7261 acttcataat cgtttgcaat tcagggcttg atctacacat taccctgtta tccctattaa
     7321 ttaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7381 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7441 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7501 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7561 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7621 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7681 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7741 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7801 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7861 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7921 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7981 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     8041 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     8101 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     8161 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     8221 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     8281 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     8341 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     8401 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     8461 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     8521 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     8581 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8641 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8701 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8761 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8821 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8881 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8941 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     9001 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     9061 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     9121 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgaacga
     9181 agcatctgtg cttcattttg tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac
     9241 aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc tattttacca
     9301 acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc gctaattttt
     9361 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt
     9421 taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat
     9481 ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc
     9541 tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct
     9601 attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa
     9661 gctgcgggtg cattttttca agataaaggc atccccgatt atattctata ccgatgtgga
     9721 ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat
     9781 tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc
     9841 gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta
     9901 atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga
     9961 aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt
    10021 ttgagcaatg tttgtggaag cggtattcgc aatattttag tagctcgtta cagtccggtg
    10081 cgtttttggt tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa agcgctctga
    10141 agttcctata ctttctagag aataggaact tcggaatagg aacttcaaag cgtttccgaa
    10201 aacgagcgct tccgaaaatg caacgcgagc tgcgcacata cagctcactg ttcacgtcgc
    10261 acctatatct gcgtgttgcc tgtatatata tatacatgag aagaacggca tagtgcgtgt
    10321 ttatgcttaa atgcgtactt atatgcgtct atttatgtag gatgaaaggt agtctagtac
    10381 ctcctgtgat attatcccat tccatgcggg gtatcgtatg cttccttcag cactaccctt
    10441 tagctgttct atatgctgcc actcctcaat tggattagtc tcatccttca atgctatcat
    10501 ttcctttgat attggatcat actaagaaac cattattatc atgacattaa cctataaaaa
    10561 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg agcgcgtttc
    10621 ggtgatgacg gtgaaaacct cggcgcgcca ttaccctgtt atccctaata tacaacgcaa
    10681 agaagctgga aaattggcga gggaatcctg tttctgtcta tccaagaatg ggcatgaggt
    10741 ggcaaccgtc gtgctagcgt acagggtgca ctttgtaacc atttgggaca ccggacactc
    10801 gctgttttcg aaattaccct ttaagcgcgg gtattgaacc aggcttatgc ccaagatcgt
    10861 agcaagcatt taactgtggg aatactcagg tatcgtaaga tgcaagagtt cgaatctctt
    10921 agcaaccatt atttttttcc tcaacataac gagaacacac aggggcgcta tcgcacagaa
    10981 tcaaattcga tgactggaaa ttttttgtta atttcagagg tcgcctgacg catatacctt
    11041 tttcaactga aaaattggga gaaaaaggaa aggtgagagc gccggaaccg gcttttcata
    11101 tagaatagag aagcgttcat gactaaatgc ttgcatcaca atacttgaag ttgacaatat
    11161 tatttaagga cctattgttt tttccaatag gtggttagca atcgtcttac tttctaactt
    11221 ttcttacctt ttacatttca gcaatatata tatatatatt tcaaggatat accattctaa
    11281 tgtctgcccc taagaagatc gtcgttttgc caggtgacca cgttggtcaa gaaatcacag
    11341 ccgaagccat taaggttctt aaagctattt ctgatgttcg ttccaatgtc aagttcgatt
    11401 tcgaaaatca tttaattggt ggtgctgcta tcgatgctac aggtgttcca cttccagatg
    11461 aggcgctgga agcctccaag aaggctgatg ccgttttgtt aggtgctgtg ggtggtccta
    11521 aatggggtac cggtagtgtt agacctgaac aaggtttact aaaaatccgt aaagaacttc
    11581 aattgtacgc caacttaaga ccatgtaact ttgcatccga ctctctttta gacttatctc
    11641 caatcaagcc acaatttgct aaaggtactg acttcgttgt tgtcagagaa ttagtgggag
    11701 gtatttactt tggtaagaga aaggaagacg atggtgatgg tgtcgcttgg gatagtgaac
    11761 aatacaccgt tccagaagtg caaagaatca caagaatggc cgctttcatg gccctacaac
    11821 atgagccacc attgcctatt tggtccttgg ataaagctaa tgttttggcc tcttcaagat
    11881 tatggagaaa aactgtggag gaaaccatca agaacgaatt ccctacattg aaggttcaac
    11941 atcaattgat tgattctgcc gccatgatcc tagttaagaa cccaacccac ctaaatggta
    12001 ttataatcac cagcaacatg tttggtgata tcatctccga tgaagcctcc gttatcccag
    12061 gttccttggg tttgttgcca tctgcgtcct tggcctcttt gccagacaag aacaccgcat
    12121 ttggtttgta cgaaccatgc cacggttctg ctccagattt gccaaagaat aaggtcaacc
    12181 ctatcgccac tatcttgtct gctgcaatga tgttgaaatt gtcattgaac ttgcctgaag
    12241 aaggtaaggc cattgaagat gcagttaaaa aggttttgga tgcaggtatc agaactggtg
    12301 atttaggtgg ttccaacagt accaccgaag tcggtgatgc tgtcgccgaa gaagttaaga
    12361 aaatccttgc ttaaaaagat tctctttttt tatgatattt gtacataaac tttataaatg
    12421 aaattcataa tagaaacgac acgaaattac aaaatggaat atgttcatag ggtagacgaa
    12481 actatatacg caatctacat acatttatca agaaggagaa aaaggaggat gtaaaggaat
    12541 acaggtaagc aaattgatac taatggctca acgaaatgac ggtatcgata agcttgatat
    12601 cgaattcctg cagcccgggg gatccactag ttctagatta attaatatct aaaaattgcc
    12661 ttatgatccg tctctccggt tacagcctgt gtaactgatt aatcctgcct ttctaatcac
    12721 cattctaatg ttttaattaa gggattttgt cttcattaac ggctttcgct cataaaaatg
    12781 ttatgacgtt ttgcccgcag gcgggaaacc atccacttca cgagactgat ctcctctgcc
    12841 ggaacaccgg gcatctccaa cttataagtt ggagaaataa gagaatttca gattgagaga
    12901 atgaaaaaaa aaaaaaaaaa aaggcagagg agagcataga aatggggttc actttttggt
    12961 aaagctatag catgcctatc acatataaat agagtgccag tagcgacttt tttcacactc
    13021 gaaatactct tactactgct ctcttgttgt ttttatcact tcttgtttct tcttggtaaa
    13081 tagaatatca agctacaaaa agcatacaat caactatcaa ctattaacta tatcgtaata
    13141 c
//
