LOCUS       sequence-211493-        7157 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for 3' FLAG tag of human ZNF511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7157)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 7157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: pFETCh_ZNF511
FEATURES             Location/Qualifiers
     source          1..7157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    85..106
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(121..126,127..144,145..151)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    159..175
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     164..186
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     183..199
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     183..199
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        376..578
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     529..549
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     575..599
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             675..1805
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     CDS             2934..2957
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2961..2984
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2988..3011
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3027..3083
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3096..3890
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3150..3169)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3760..3779
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4753..4769)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4762..4784)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(4988..5803)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNILQFHLMLDEFF"
     primer_bind     5711..5730
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5795..5814
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6183..6771
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6672..6691
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6925..6942
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
       61 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
      121 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
      181 cacaggaaac agctatgacc atgattacgc caagcttgca tgcctgcagg tcgactctag
      241 aggatccccg ggtaccgagc tcgaattagc ttgcatgcct gcaggtcaat tccctggcat
      301 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      361 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      421 gactcacggg gatttcaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      481 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      541 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
      601 cctggagacg ccatccacgc tgttttgacc tccataggag acaccgggac cgatccagcc
      661 tggggatccc gaccatggct tcgtacccct gccatcaaca cgcgtctgcg ttcgaccagg
      721 ctgcgcgttc tcgcggccat agcaaccgac gtacggcgtt gcgccctcgc cggcagcaag
      781 aagccacgga agtccgcctg gagcagaaaa tgcccacgct actgcgggtt tatatagacg
      841 gtcctcacgg gatggggaaa accaccacca cgcaactgct ggtggccctg ggttcgcgcg
      901 acgatatcgt ctacgtaccc gagccgatga cttactggca ggtgctgggg gcttccgaga
      961 caatcgcgaa catctacacc acacaacacc gcctcgacca gggtgagata tcggccgggg
     1021 acgcggcggt ggtaatgaca agcgcccaga taacaatggg catgccttat gccgtgaccg
     1081 acgccgttct ggctcctcat atcggggggg aggctgggag ctcacatgcc ccgcccccgg
     1141 ccctcaccct catcttcgac cgccatccca tcgccgccct cctgtgctac ccggccgcgc
     1201 gataccttat gggcagcatg accccccagg ccgtgctggc gttcgtggcc ctcatcccgc
     1261 cgaccttgcc cggcacaaac atcgtgttgg gggcccttcc ggaggacaga cacatcgacc
     1321 gcctggccaa acgccagcgc cccggcgagc ggcttgacct ggctatgctg gccgcgattc
     1381 gccgcgttta cgggctgctt gccaatacgg tgcggtatct gcagggcggc gggtcgtggc
     1441 gggaggattg gggacagctt tcggggacgg ccgtgccgcc ccagggtgcc gagccccaga
     1501 gcaacgcggg cccacgaccc catatcgggg acacgttatt taccctgttt cgggcccccg
     1561 agttgctggc ccccaacggc gacctgtaca acgtgtttgc ctgggccttg gacgtcttgg
     1621 ccaaacgcct ccgtcccatg cacgtcttta tcctggatta cgaccaatcg cccgccggct
     1681 gccgggacgc cctgctgcaa cttacctccg ggatggtcca gacccacgtc accacccccg
     1741 gctccatacc gacgatctgc gacctggcgc gcacgtttgc ccgggagatg ggggaggcta
     1801 actgaggatc ccacgtcact attgtatact ctatattata ctctatgtta tactctgtaa
     1861 tcctactcaa taaacgtgtc acgcctgtga aaccgtacta agtctcccgt gtattcttat
     1921 caccatcagg tgacatcctc gcccaggctg tcaatcatgc cggtatcgat tccagtagca
     1981 ccggccccac gctgacaacc cactcttgca gcgttagcag cgcccctctt aacaagccga
     2041 cccccaccag cgtcgcggtt actaacactc ctctccccga cctgcagccc agctgaatgc
     2101 caagcaccac tcacttttcc tcattgtctg agtgggatgg aaagagtctc acttgcagtt
     2161 gcttcagtca caacccaggc gtctgcctta atagcatcac ctgtgccgtc tgcgtttcta
     2221 gcgtcacctg tgccgtctgc gtttctagcg tcacctgtgc cgtctgcctt cctagcatga
     2281 cctgtgccgt ctgcctttct agcatgacct gtactgtctg cctttctagc gtcacctgtg
     2341 ccgtctgcct ttctagttaa agttgatttt aaattaattt gggaaaaacg gtgaacaagt
     2401 ggggaaaaac atcaccaaag actccaaaca ggtggaacga tgaacttaat gttccctgca
     2461 ccctccttac agatgcctgg actttggcct gtgaatgagc cccggggggc tgggacagta
     2521 ggggttttca tggaatggac atggggtagc aggaagtctc agccccagag ggcttggcag
     2581 gagccgccct ctctgcggag ttcttcccag cgggtgtgcg ttggtggctg gcggggaccc
     2641 cccacaggaa ctaggtctca ggctgtggaa agggagctgc cccagggccc aggagggccg
     2701 gctctgcatt ccgcatgttc ccagagtgaa agagaatgag aaggagacac acaagtgctg
     2761 ttatgacctg ggttgttggt tggcaaatac agcatctaat agaaactttc ttccatccca
     2821 gaataccctc taccatctgc tttggtcagg gtgccgctcg aggatttaaa agcaacaaga
     2881 agaaaaccaa acaatgcggg agcggaggag gttccggagg aggtggttct ggagattaca
     2941 aggatgacga cgataagggc gattacaagg atgacgacga taagggagat tacaaggatg
     3001 acgacgataa ggtttcagga agcggagcta ctaacttcag cctgctgaag caggctggag
     3061 acgtggagga gaaccctgga cctggatcgt ttcgcatgat tgaacaagat ggattgcacg
     3121 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     3181 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     3241 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     3301 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     3361 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     3421 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     3481 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     3541 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     3601 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     3661 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     3721 gtggccggct gggtgtggcc gaccgctatc aggacatagc gttggctacc cgtgatattg
     3781 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     3841 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga ttcgaacatc
     3901 atggggggca gtcaccactg ctgggcgtgg catccgcctt gggcctttct ccgaccttct
     3961 ggtgtggccg ccctgggggc caggccctcg ccatcctccc catccttgtt cctcagcaaa
     4021 tggcattagc agctgtcatc agaagctgtg agggacccac agattttgga aacgacctgg
     4081 acacactatt gggaaggaga tgtggacggc ctgtctcctc ctgcagggcc caccctaaga
     4141 atgtattttt aaacacatga aataagtatt tttcactgat ggtcttgagt cttcatttct
     4201 gtgttgagtc aggtgcggag tggttattgt tacccatggg tattctgttt tccaccctca
     4261 gttttcagga agatttataa agtcctggca cccctcagag gtgggtgatg gggtaggata
     4321 cagcactcca cctcgtcccg gacctcctgg ctttgggagc cacacccagt tgtccttgcc
     4381 acaccactta gggagtagac ctggtggggg taggatctga tgcccacagg gaggggcact
     4441 ggctctgtgg gcatggggtg cttgggggtg tgtgggatgc tgggggagtg gggggtgctg
     4501 ccccagaggg tgcaggatcc atcactagtg ccagcgggga ccacagagcc tgcatcagga
     4561 aatgcaggcc aggagattgg ggctggcatt ggattgcaga gatacactgg gggcagtgtg
     4621 tcctggagaa atcactctgt tttgataaaa ttagggcgct cagagactcc cgaccacgtc
     4681 ttcctccagc ggccctgttc cttggaaagt cctctccact gactgtagcc tccaattcac
     4741 tggccgtcgt tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     4801 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     4861 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgcg atttattcaa
     4921 caaagccgcc gtcccgtcaa gtcagcgtaa tgctctgcca gtgttacaac caattaacca
     4981 attctgatta gaaaaactca tcgagcatca aatgaaactg caatatattc atatcaggat
     5041 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
     5101 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa
     5161 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
     5221 tgacgactga atccggtgag aatggcaaaa gcttatgcat ttctttccag acttgttcaa
     5281 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc
     5341 gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag
     5401 gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat
     5461 caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc
     5521 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca
     5581 gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt
     5641 tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt
     5701 gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta
     5761 atcgcggctt cgagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac
     5821 tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt
     5881 aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact tttgctgagt
     5941 tgaaggatca gatcacgcat cttcccgaca acgcagaccg ttccgtggca aagcaaaagt
     6001 tcaaaatcac caactggtcc acctacaaca aagctctcat caaccgtggc tccctcactt
     6061 tctggctgga tgatggggcg attcaggcct ggtatgagtc agcaacacct tcttcacgag
     6121 gcagacctct cgacggagtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6181 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6241 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6301 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     6361 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6421 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6481 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6541 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6601 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6661 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6721 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6781 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     6841 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     6901 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agaagctcgc
     6961 acattcagca gcgtttttca gcgcgttttc gatcaacgtt tcaatgttgg tatcaacacc
     7021 aggtttaact ttgaacttat cggcactgac ggttactgat tttgaacttt tgctttgcca
     7081 cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca gcaaaagttc
     7141 ccaatacgca aaccgcc
//
