LOCUS       sequence-211498-       11203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of xCas9(3.7)RA in 
            mammalian cells (codon optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11203)
  AUTHORS   Zafra MP, Schatoff EM, Katti A, Foronda M, Breinig M, Schweitzer AY,
            Simon A, Han T, Goswami S, Montgomery E, Thibado J, Kastenhuber ER, 
            Sanchez-Rivera FJ, Shi J, Vakoc CR, Lowe SW, Tschaharganeh DF, Dow 
            LE
  TITLE     Optimized base editors enable efficient editing in cells, organoids 
            and mice.
  JOURNAL   Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
  PUBMED    29969439
REFERENCE   2  (bases 1 to 11203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194."
COMMENT     Sequence Label: pLenti-xCas9RA-P2A-Puro
FEATURES             Location/Qualifiers
     source          1..11203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..239
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             240..420
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    467..592
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1085..1318
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1503..1547
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1731..1848
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1917..2128
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2148..2157
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2157..2180
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2187..2207
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2232..6332
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDTKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKLYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGIIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEKVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGDQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFIQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGVLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6333..6380
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6396..6452
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6453..7052
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6453..6472)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6949..6969
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7068..7656
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7121..7141)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7539..7550)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7728..7961
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8039..8160
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8076..8095)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8130..8149
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8200..8335
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8355..8374)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8356..8374)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8384..8401)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8384..8400)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8393..8415)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8542..8997
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8629..8648)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8839..8860
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9023..9127
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9128..9196,9197..9988)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9346..9365)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10159..10747
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10648..10667
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10901..10918
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11035..11056
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11071..11076,11077..11094,11095..11101)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11109..11125
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11114..11136
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11133..11149
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11133..11149
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11168..11188
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11170..11188
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
       61 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
      121 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
      181 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
      241 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
      301 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
      361 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
      421 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
      481 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
      541 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
      601 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
      661 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
      721 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
      781 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
      841 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
      901 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
      961 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     1021 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     1081 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     1141 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     1201 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     1261 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     1321 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     1381 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     1441 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     1501 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     1561 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     1621 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     1681 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cagaccacaa ttttaaaaga
     1741 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     1801 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     1861 agggacagca gagatccact ttggcgccgg ctcgagtggc tccggtgccc gtcagtgggc
     1921 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
     1981 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     2041 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     2101 tcgcaacggg tttgccgcca gaacacaggt gtcgtgacgc gggatccgcc accatggatt
     2161 acaaagacga tgacgataag atggccccaa agaagaagcg gaaggtcggt atccacggag
     2221 tcccagcagc cgacaagaag tacagcatcg gcctggacat cggcaccaac tctgtgggct
     2281 gggccgtgat caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca
     2341 ccgaccggca cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa
     2401 cagccgaggc cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc
     2461 ggatctgcta tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct
     2521 tccacagact ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca
     2581 tcttcggcaa catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc
     2641 tgagaaagaa actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc
     2701 tggcccacat gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca
     2761 acagcgacgt ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg
     2821 aaaaccccat caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca
     2881 agagcagacg gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt
     2941 tcggaaacct gattgccctg agcctgggcc tgacccccaa cttcaagagc aacttcgacc
     3001 tggccgagga taccaaactg cagctgagca aggacaccta cgacgacgac ctggacaacc
     3061 tgctggccca gatcggcgac cagtacgccg acctgtttct ggccgccaag aacctgtccg
     3121 acgccatcct gctgagcgac atcctgagag tgaacaccga gatcaccaag gcccccctga
     3181 gcgcctctat gatcaagctg tacgacgagc accaccagga cctgaccctg ctgaaagctc
     3241 tcgtgcggca gcagctgcct gagaagtaca aagagatttt cttcgaccag agcaagaacg
     3301 gctacgccgg ctacattgac ggcggagcca gccaggaaga gttctacaag ttcatcaagc
     3361 ccatcctgga aaagatggac ggcaccgagg aactgctcgt gaagctgaac agagaggacc
     3421 tgctgcggaa gcagcggacc ttcgacaacg gcatcatccc ccaccagatc cacctgggag
     3481 agctgcacgc cattctgcgg cggcaggaag atttttaccc attcctgaag gacaaccggg
     3541 aaaagatcga gaagatcctg accttccgca tcccctacta cgtgggccct ctggccaggg
     3601 gaaacagcag attcgcctgg atgaccagaa agagcgagga aaccatcacc ccctggaact
     3661 tcgagaaggt ggtggacaag ggcgcttccg cccagagctt catcgagcgg atgaccaact
     3721 tcgataagaa cctgcccaac gagaaggtgc tgcccaagca cagcctgctg tacgagtact
     3781 tcaccgtgta taacgagctg accaaagtga aatacgtgac cgagggaatg agaaagcccg
     3841 ccttcctgag cggcgaccag aaaaaggcca tcgtggacct gctgttcaag accaaccgga
     3901 aagtgaccgt gaagcagctg aaagaggact acttcaagaa aatcgagtgc ttcgactccg
     3961 tggaaatctc cggcgtggaa gatcggttca acgcctccct gggcacatac cacgatctgc
     4021 tgaaaattat caaggacaag gacttcctgg acaatgagga aaacgaggac attctggaag
     4081 atatcgtgct gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa
     4141 cctatgccca cctgttcgac gacaaagtga tgaagcagct gaagcggcgg agatacaccg
     4201 gctggggcag gctgagccgg aagctgatca acggcatccg ggacaagcag tccggcaaga
     4261 caatcctgga tttcctgaag tccgacggct tcgccaacag aaacttcatc cagctgatcc
     4321 acgacgacag cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg
     4381 atagcctgca cgagcacatt gccaatctgg ccggcagccc cgccattaag aagggcatcc
     4441 tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat gggccggcac aagcccgaga
     4501 acatcgtgat cgaaatggcc agagagaacc agaccaccca gaagggacag aagaacagcc
     4561 gcgagagaat gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag
     4621 aacaccccgt ggaaaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga
     4681 atgggcggga tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc gactacgatg
     4741 tggaccatat cgtgcctcag agctttctga aggacgactc catcgacaac aaggtgctga
     4801 ccagaagcga caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga
     4861 agatgaagaa ctactggcgg cagctgctga acgccaagct gattacccag agaaagttcg
     4921 acaatctgac caaggccgag agaggcggcc tgagcgaact ggataaggcc ggcttcatca
     4981 agagacagct ggtggaaacc cggcagatca caaagcacgt ggcacagatc ctggactccc
     5041 ggatgaacac taagtacgac gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc
     5101 tgaagtccaa gctggtgtcc gatttccgga aggatttcca gttttacaaa gtgcgcgaga
     5161 tcaacaacta ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga accgccctga
     5221 tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg
     5281 tgcggaagat gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc aagtacttct
     5341 tctacagcaa catcatgaac tttttcaaga ccgagattac cctggccaac ggcgagatcc
     5401 ggaagcggcc tctgatcgag acaaacggcg aaaccgggga gatcgtgtgg gataagggcc
     5461 gggattttgc caccgtgcgg aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga
     5521 ccgaggtgca gacaggcggc ttcagcaaag agtctatcct gcccaagagg aacagcgata
     5581 agctgatcgc cagaaagaag gactgggacc ctaagaagta cggcggcttc gacagcccca
     5641 ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc aagaaactga
     5701 agagtgtgaa agagctgctg gggatcacca tcatggaaag aagcagcttc gagaagaatc
     5761 ccatcgactt tctggaagcc aagggctaca aagaagtgaa aaaggacctg atcatcaagc
     5821 tgcctaagta ctccctgttc gagctggaaa acggccggaa gagaatgctg gcctctgccg
     5881 gcgtgctgca gaagggaaac gaactggccc tgccctccaa atatgtgaac ttcctgtacc
     5941 tggccagcca ctatgagaag ctgaagggct cccccgagga taatgagcag aaacagctgt
     6001 ttgtggaaca gcacaagcac tacctggacg agatcatcga gcagatcagc gagttctcca
     6061 agagagtgat cctggccgac gctaatctgg acaaagtgct gtccgcctac aacaagcacc
     6121 gggataagcc catcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc
     6181 tgggagcccc tgccgccttc aagtactttg acaccaccat cgaccggaag aggtacacca
     6241 gcaccaaaga ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga
     6301 cacggatcga cctgtctcag ctgggaggcg acaagcgtcc tgctgctact aagaaagctg
     6361 gtcaagctaa gaaaaagaaa gctagcggca gcggcgccac caacttcagc ctgctgaagc
     6421 aggccggcga cgtggaggag aaccccggcc ccatgaccga gtacaagccc acggtgcgcc
     6481 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     6541 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     6601 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     6661 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     6721 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     6781 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggag
     6841 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     6901 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     6961 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     7021 cctggtgcat gacccgcaag cccggtgcct gaacgcgtta agtcgacaat caacctctgg
     7081 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     7141 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     7201 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     7261 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     7321 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     7381 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     7441 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     7501 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     7561 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     7621 agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta
     7681 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     7741 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     7801 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7861 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7921 atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata gtagttcatg
     7981 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     8041 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     8101 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     8161 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     8221 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     8281 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     8341 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     8401 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     8461 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     8521 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     8581 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     8641 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     8701 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     8761 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     8821 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     8881 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     8941 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     9001 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     9061 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     9121 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     9181 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     9241 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     9301 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     9361 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     9421 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     9481 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     9541 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     9601 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     9661 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     9721 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     9781 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     9841 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     9901 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     9961 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
    10021 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
    10081 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
    10141 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
    10201 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
    10261 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
    10321 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
    10381 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
    10441 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
    10501 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
    10561 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
    10621 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
    10681 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
    10741 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
    10801 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
    10861 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
    10921 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
    10981 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
    11041 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
    11101 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
    11161 caagcgcgca attaaccctc actaaaggga acaaaagctg gag
//
