LOCUS       sequence-211500-       15984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NRPS module containing CATR domains with C-terminal 6His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 15984)
  AUTHORS   Schneider BA, EP Balskus
  TITLE     Discovery of small molecule protease inhibitors by investigating a 
            widespread human gut bacterial biosynthetic pathway,
  JOURNAL   Tetrahedron
REFERENCE   2  (bases 1 to 15984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Tetrahedron"
COMMENT     Sequence Label: pET29b-RupA_C2-A2-T2-R-CHis
FEATURES             Location/Qualifiers
     source          1..15984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        631..934
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        935..1138
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1089..1109
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1177..1186
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1183..2610
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     polyA_signal    3554..3675
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3591..3610)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3645..3664
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3682..4137)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     terminator      complement(4174..4221)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     4217..4235
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(4288..4305)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(8584..8628)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     RBS             8639..8661
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    8676..8700
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(8700..8719)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8701..8719)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8807..8826)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        9032..9109
                     /gene="lacI"
                     /label=lacI promoter
     CDS             9110..10192
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(9129..9148)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             11001..11192
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(11186..11208)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11294..11433
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11349..11368
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11448..11465)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11619..12207)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12318..12337)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             12329..13144
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             12329..13144
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(12402..12421)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      complement(13236..13691)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        13718..13822
                     /gene="bla"
                     /label=AmpR promoter
     promoter        13824..14181
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        13824..14015
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(13847..13867)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      14032..14167
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             14216..15010
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             14216..15010
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(14270..14289)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     14880..14899
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(15198..15217)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    15242..15289
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
       61 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
      121 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
      181 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
      241 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
      301 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
      361 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
      421 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
      481 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
      541 tctgtggata accgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc
      601 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      661 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      721 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      781 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      841 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      901 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
      961 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     1021 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     1081 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg
     1141 tttagtgaac cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag
     1201 gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac
     1261 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     1321 ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc
     1381 atgtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg
     1441 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg
     1501 accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc
     1561 ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     1621 tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg
     1681 aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag
     1741 cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac
     1801 gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg
     1861 gggcatggca ccggcagcac cggcagcggc agctccggca ccgcctcctc cgaggacaac
     1921 aacatggccg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac
     1981 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     2041 gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc
     2101 cagttcatgt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag
     2161 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt
     2221 ctggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag
     2281 atgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg
     2341 gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag
     2401 gccctgaagc taaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc
     2461 aagaagcccg tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc
     2521 cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg
     2581 ttcctgtacg gcatggacga gctgtacaag tccggactca gatctggcgg tatgggtgag
     2641 ttcaacgaga agaagacaac atgtggcacc gtttgcctca agtacctgct gtttacctac
     2701 aattgctgct tctggctggc tggcctggct gtcatggcag tgggcatctg gacgctggcc
     2761 ctcaagagtg actacatcag cctgctggcc tcaggcacct acctggccac agcctacatc
     2821 ctggtggtgg cgggcactgt cgtcatggtg actggggtct tgggctgctg cgccaccttc
     2881 aaggagcgtc ggaacctgct gcgcctgtac ttcatcctgc tcctcatcat ctttctgctg
     2941 gagatcatcg ctggtatcct cgcctacgcc tactaccagc agctgaacac ggagctcaag
     3001 gagaacctga aggacaccat gaccaagcgc taccaccagc cgggccatga ggctgtgacc
     3061 agcgctgtgg accagctgca gcaggagttc cactgctgtg gcagcaacaa ctcacaggac
     3121 tggcgagaca gtgagtggat ccgctcacag gaggccggtg gccgtgtggt cccagacagc
     3181 tgctgcaaga cggtggtggc tctttgtgga cagcgagacc atgcctccaa catctacaag
     3241 gtggagggcg gctgcatcac caagttggag accttcatcc aggagcacct gagggtcatt
     3301 ggggctgtgg ggatcggcat tgcctgtgtg caggtctttg gcatgatctt cacgtgctgc
     3361 ctgtacagga gtctcaagct ggagcactac tgagaattct gcagtcgacg gtaccgcggg
     3421 cccgggatcc accggatcta gataactgat cataatcagc cataccacat ttgtagaggt
     3481 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     3541 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     3601 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     3661 catcaatgta tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     3721 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     3781 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     3841 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     3901 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     3961 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     4021 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     4081 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     4141 attcgccaat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga cccgtttaga
     4201 ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact cagcttcctt
     4261 tcgggctttg ttagcagccg gatctcagtg gtggtggtgg tggtgctcga gatcgaagaa
     4321 accaagacct cttaatgcgt tgatgaatcg tttcatatag tcaagcgagg taacgggcca
     4381 cacaaatccc attctgtaaa gaacctgcat tgtatatgtg ttgctcttgc cgactgtaaa
     4441 tgtcttttga ccgtgagcca tgttctgata tgcaatcata ctcgaaagaa tctttgcttt
     4501 ttcgggatcc tgctcagctc tgtcaagtgc aatcatatat tcctcatact ctgcaccctg
     4561 tgaatgaagt ccgattttgt tcatctcggt gtagaggtcg cccataatga gagtatggtt
     4621 gttgaacggg tggaacacgg tgcaatcctt cggagcctgt gcaagcagaa gaattgcctt
     4681 tgccacaaaa tcaatcggag aaagctcaaa cggcatttcc attgcctcat acggatattt
     4741 gccgataagg agtgtagatt tgagtctgcc cataaatgtg ttggtggtga aattaatctg
     4801 atattcaccg tcgctgtttc ttgcggcaag tgtgcctaca cgcataatct ttgcgttaaa
     4861 gccctttgaa acctcttcaa gaatcgccct ttccgcaagg aactttgaca gggtgtactt
     4921 tgaaccctca tgctgaccaa agtaaagttg attttctttg agcttgtcaa cagaaccttg
     4981 ttcgcccaca aacataccgc ctacgctcat tgttgacaca tgaacaagcc ttgcattagc
     5041 ctttttgcag aagtcaagca cattgagagt accgtaaagg tttacatctt caatgtctgt
     5101 accctttgaa aagtgcttaa catttgcggc acagttgatg accgtgttaa tgtccttgtc
     5161 aataaattta tcgaatgatt cacggtttgt aacatcaccg ctgataacgg taactctgtc
     5221 gggatatctt tctttcagac tttcttcaaa gtagtaatag tagattgagt tcattctgtt
     5281 ttcggcagga ttattgtttt tgtcacgaat catgcagtaa accttgccgt tatacttgtg
     5341 aagaagttcg tagagaatgt gtactccgag gaagccggca gagcctgtca gaagaacatt
     5401 gccgagtttt tgaagctcac cgtttttaaa tgtatcaata ttattctttt caagaacttt
     5461 attgatattt gtataatcgt agtccgaaag attttcaagg tcgtcttcgg ctgaatcatc
     5521 cttgctcaca aattttgcaa gagtctgcgg agtaggattt gcaaaaatgt cgctgtatgc
     5581 aacctcaata tttttctttg atgcggagat tacaattctt gttgctgtaa gcgatgttcc
     5641 gccgaggtca aagaagttat cgtctgcaaa aacattgtca agttcaagaa tttctgcaaa
     5701 gatgtcgcag aatgtttttt caagttcatt cttcggcaac cgacctcttt cggatttgtt
     5761 gcttttaggt tcgggaagaa cctttgtgtt gaccttgccg tttggtgtga gaggcatttc
     5821 gtcaagctga acatatgcgc tcggaaccat ataatccgtg agagcttttg caagctcaga
     5881 cttaatttca tttggctcaa tctgtctgtc ggctgtatag taaacgcaga ttgcgtcagc
     5941 cttgccgacc tttcttatga gagcaattcc gctcttgatt ccgtcaactg cggtcagaca
     6001 cttttcaatt tcaccgagtt caattcttaa tcctctcagc ttaacctgat tgtcggttct
     6061 gccgagaata ataacatcgc cgtctgttgt ccattttgcg tagtcgcccg agcggtaggt
     6121 gcgttcaccg ttaaattcga tgaatgcctt tgcagtctgt tcgggaagtt tgttgtagcc
     6181 gagtgccaca ccgcatccct gaatgagaag ctcgcccaca actccgattg gaaggagatt
     6241 gttgtctgag tcaacgatat attccgtgta gttgcgtaac ggcctgccga ttgaaatttc
     6301 gtctgcattc gtaagatttt ttgcgtttga agaaaccgtg atttcagtcg gaccgtaggt
     6361 gttgattatt gttgcgttcg tcttttcacg cagtatcctt aatagcttgt cgggatattt
     6421 ttcaccgccc gagagaataa ccttgcagtt tgccattgct tctgcaaatt cgtcaagctc
     6481 catatattgc aaaagtcttg acggtgtaga gttgaacgca tcaacattgt tttctttaaa
     6541 gaattctgca agtgacatcg ggtttaccgt ctgttcttca cttgcaaaaa cgagtgtaag
     6601 accgttgcag agcgacagca tggtttcctt tagcgacata tcaaagctga ttgtagttac
     6661 agagccgtat gcgtgacagt tgtcaacaac ctctttaacc tgaatgtttg aatcgctgta
     6721 tgcaaggtag tttgcaatgc cgatgtggcg tagcataacg ccctttggct tacctgttga
     6781 acctgaggtg tagataaggt acgcaaggtc gtcaggctgt acatcaacat tcggattttc
     6841 ttcattattg cctgaaagaa gttttgaaac atcaaccgtg cggtcatatt tattgagcag
     6901 ttcgccgttc gtaattacaa agtctgcgtc actgtcctca ataatactgt cgattcgctc
     6961 cttcggatag tcgggacatg acggaataaa cgcaccgccg gccttgagaa taccgaaaag
     7021 tgatatgaag aattttgagg ttctttcaag cagaataaga acctttccgc ctttttcaag
     7081 acctttttca atgagtgagt ttgccgtgat atttgcaaga cggtcaagct ctttataggt
     7141 tagtttaccg tcacaagccg aaagagcaat tctgtcggga gtttttgcaa cctgttcttc
     7201 aaacattttg tgaagaagtt ttgtcttaac gggagcaatt tctgttgact taaagctttc
     7261 aagctgagca attgcggcgt tgtcaaggag tgaaaccttt ctgattttgc cgtttggatt
     7321 ttcgataata tgttcaacag cgcagagaac ggattttgca agcgttctca taagctcacc
     7381 gctgtaaagt gcgtcattgt actgaaccac aacgctcggc ttgcccttgt aatcctcaat
     7441 gtgaacggct gttttaaatt ttgcagtatt catttcaagg taatcacgga caacggcttt
     7501 gccgtcaatt tcaaggttat caacaacacc aagctgatat tcgtacataa tgttcggcgc
     7561 atagccgtat tttgcacaaa gctgtgcata gggataaatt tcgttttcaa ttgaatccgt
     7621 gaaaacagct ttgctttttt caacaaattc aagtgaggtg atgtcctcaa tttcaatgcc
     7681 gagcgcaaga gtttttacaa acataccaaa gcagtttgta agccgcatat cgcttctgcc
     7741 gttgctgatt gtgctgatgt aaacatttct gctgttcaca aatcttgaaa cagcgtagaa
     7801 tgtgccggca aggaaaaggt gggcgggagt gattccgtgc tgtgaacaga agttttcaac
     7861 ccttgccata tcaacgggga ctgcctgaga tgcaagagca ccgtcctctg catgacctct
     7921 gaggtcagcc gtgatttcgc ttgcagattc aaaacttttc atcatattgt cgaagaactt
     7981 ttcagcattt ctaaattcat cactgctttc agccttaatt tcattctctg cataatcaaa
     8041 gtaggtgtag ctttcaggct caatattacc cccttcgtac agcgttttaa gctgttcaaa
     8101 aaaaagtgca acggacgcac cgtcaaagat aaggtggtgg aaatctgaaa gcagatatac
     8161 cgccttttcg gtttctgcaa ccgagattct gaacagcggt gatttcataa gattgtgcgg
     8221 ttttacaaaa tcctttttgt aggattcaag ctgttcttcc gtaagctttt caacggggac
     8281 attcggtttt gcagagtcaa catatgcctg ctcaatatca tcgttttcaa gtgaaaggtg
     8341 agtcataata tatgggtgag ccttgattac ctttacaacg cagtcggcaa gcttttgtgc
     8401 atcgactgat tttgaaaatt tgagaattga cggaatattg taaagtgtgc tgtacggatt
     8461 tttcatacag tcaatataca cgccgagctg agtctttgaa agaggataga gagccttatg
     8521 ttctttcttt tcttcttttt tgacagtttt ctgatttgcc gtaccgctga acattagatc
     8581 tgggctgtcc atgtgctggc gttcgaattt agcagcagcg gtttctttca tatgtatatc
     8641 tccttcttaa agttaaacaa aattatttct agaggggaat tgttatccgc tcacaattcc
     8701 cctatagtga gtcgtattaa tttcgcggga tcgagatcga tctcgatcct ctacgccgga
     8761 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
     8821 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
     8881 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
     8941 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
     9001 gagtcgcata agggagagcg tcgagatccc ggacaccatc gaatggcgca aaacctttcg
     9061 cggtatggca tgatagcgcc cggaagagag tcaattcagg gtggtgaatg tgaaaccagt
     9121 aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt
     9181 gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga
     9241 gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat
     9301 tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa
     9361 atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt
     9421 cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat
     9481 taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc
     9541 ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga
     9601 agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct
     9661 gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata
     9721 tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc
     9781 cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt
     9841 tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt
     9901 tggtgcggac atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc
     9961 gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt
    10021 gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt
    10081 gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    10141 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    10201 caattaatgt aagttagctc actcattagg caccgggatc tcgaccgatg cccttgagag
    10261 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
    10321 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt
    10381 tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg
    10441 gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg
    10501 agaagcaggc cattatcgcc ggcatggcgg ccccacgggt gcgcatgatc gtgctcctgt
    10561 cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga
    10621 tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat
    10681 gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca
    10741 ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat
    10801 ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc
    10861 ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa
    10921 cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatc
    10981 ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac atggcccgct
    11041 ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac
    11101 aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc agctgcctcg
    11161 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
    11221 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
    11281 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
    11341 taactatgcg gcatcagagc agattgtact gagagtgcac cattgcggtg tgaaataccg
    11401 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
    11461 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
    11521 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
    11581 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
    11641 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
    11701 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
    11761 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
    11821 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    11881 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    11941 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    12001 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
    12061 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    12121 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    12181 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    12241 gctcagtgga acgaaaactc acgttaaggg attttggtca tgaacaataa aactgtctgc
    12301 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc
    12361 taggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
    12421 taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga
    12481 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
    12541 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
    12601 tgatgatgca tggttactca ccactgcgat ccccgggaaa acagcattcc aggtattaga
    12661 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
    12721 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
    12781 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
    12841 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga
    12901 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
    12961 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
    13021 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
    13081 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
    13141 ctaagaatta attcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    13201 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
    13261 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
    13321 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
    13381 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
    13441 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
    13501 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
    13561 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
    13621 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
    13681 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
    13741 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
    13801 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
    13861 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
    13921 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
    13981 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
    14041 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
    14101 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
    14161 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat
    14221 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
    14281 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
    14341 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
    14401 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
    14461 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
    14521 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
    14581 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
    14641 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
    14701 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
    14761 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
    14821 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
    14881 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
    14941 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
    15001 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
    15061 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
    15121 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
    15181 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
    15241 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
    15301 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
    15361 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
    15421 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag
    15481 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    15541 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    15601 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    15661 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    15721 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
    15781 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    15841 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    15901 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    15961 agcttggagc gaacgaccta cacc
//
