LOCUS       sequence-211502-         222 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP from jellyfish Aquorea sp. under strong synthetic constitutive 
            promoter pFAB4026; kanamycin resistant..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 222)
  AUTHORS   Siddall A, Williams AA, Sanders J, Denton JA, Madden D, Schollar J, 
            Bryk J
  TITLE     UNIGEMS: plasmids and parts to facilitate teaching on assembly, gene
            expression control and logic in E. coli
  JOURNAL   bioRxiv 2021.06.20.449138
REFERENCE   2  (bases 1 to 222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.06.20.449138"
COMMENT     Sequence Label: p006-GFP-strong
FEATURES             Location/Qualifiers
     source          1..222
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             105..113
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tgcttattat nntccggctc gtataatgtg tggagggccc aagttcactt aaaaaggaga
       61 tcaacaatga aagcaatttt cgtactgaaa catcttaatc atgctaagga ggttttctaa
      121 tgagtaaagg agaagaactt ttcactagag ttgtcccaat tcttgttgaa ttagacggtg
      181 atgttaatgg gcacaaattt tctgtcagtg gagagggaga aa
//
