LOCUS       sequence-211514-        8859 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-regulated miR-E (miR-30 variant)-based RNAi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8859)
  AUTHORS   Fellmann C, Hoffmann T, Sridhar V, Hopfgartner B, Muhar M, Roth M, 
            Lai DY, Barbosa IA, Kwon JS, Guan Y, Sinha N, Zuber J
  TITLE     An optimized microRNA backbone for effective single-copy RNAi.
  JOURNAL   Cell Rep. 2013 Dec 26;5(6):1704-13. doi: 
            10.1016/j.celrep.2013.11.020. Epub 2013 Dec 12.
  PUBMED    24332856
REFERENCE   2  (bases 1 to 8859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.11.020"; journalName: "Cell Rep"; date: 
            "2013-12-26- 26"; volume: "5"; issue: "6"; pages: "1704-13"
COMMENT     Sequence Label: LT3REV
FEATURES             Location/Qualifiers
     source          1..8859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     121..138
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    255..276
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(291..296,297..314,315..321)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    329..345
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     334..356
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     353..369
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     353..369
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     388..408
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        390..408
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        436..662
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             663..843
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    890..1015
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1508..1741
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1926..1970
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2237..2354
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2372..2750
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    2380..2398
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2416..2434
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2452..2470
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2488..2506
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2524..2542
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2560..2578
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2596..2614
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2659..2683
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2774..3451
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(2954..2974)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     3366..3389
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        3715..4214
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3750..3773)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4147..4166
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(4237..4239,4240..4242,4243..4956)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4282..4303)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4543..4562)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4890..4911
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5018..5606
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5071..5091)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5489..5500)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5813..6046
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6118..6239
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6155..6174)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6209..6228
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6279..6414
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6434..6453)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6435..6453)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6463..6480)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6463..6479)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6472..6494)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6621..7076
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6708..6727)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6918..6939
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7102..7206
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7207..7275,7276..8067)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7425..7444)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8238..8826
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8727..8746
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
       61 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
      121 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
      181 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
      241 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
      301 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
      361 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
      421 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
      481 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
      541 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
      601 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
      661 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
      721 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
      781 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
      841 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
      901 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
      961 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     1021 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     1081 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     1141 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     1201 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     1261 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     1321 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     1381 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     1441 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     1501 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     1561 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     1621 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     1681 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     1741 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     1801 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     1861 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     1921 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     1981 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     2041 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     2101 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     2161 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc
     2221 gacggtatcg gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     2281 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     2341 aaattcaaaa ttttatcgat cctctagatt cgagtttact ccctatcagt gatagagaac
     2401 gtatgaagag tttactccct atcagtgata gagaacgtat gcagacttta ctccctatca
     2461 gtgatagaga acgtataagg agtttactcc ctatcagtga tagagaacgt atgaccagtt
     2521 tactccctat cagtgataga gaacgtatct acagtttact ccctatcagt gatagagaac
     2581 gtatatccag tttactccct atcagtgata gagaacgtat aagctttagg cgtgtacggt
     2641 gggcgcctat aaaagcagag ctcgtttagt gaaccgtcag atcgcctgga gcaattccac
     2701 aacacttttg tcttatacca actttccgta ccacttccta ccctcgtaaa gtcgagcttg
     2761 cgttggatcc accatggcct cctccgagaa cgtcatcacc gagttcatgc gcttcaaggt
     2821 gcgcatggag ggcaccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     2881 cccctacgag ggccacaaca ccgtgaagct gaaggtgacc aagggcggcc ccctgccctt
     2941 cgcctgggac atcctgtccc cccagttcca gtacggctcc aaggtgtacg tgaagcaccc
     3001 cgccgacatc cccgactaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     3061 gatgaacttc gaggacggcg gcgtggcgac cgtgacccag gactcctccc tgcaggacgg
     3121 ctgcttcatc tacaaggtga agttcatcgg cgtgaacttc ccctccgacg gccccgtgat
     3181 gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt
     3241 gctgaagggc gagacccaca aggccctgaa gctgaaggac ggcggccact acctggtgga
     3301 gttcaagtcc atctacatgg ccaagaagcc cgtgcagctg cccggctact actacgtgga
     3361 cgccaagctg gacatcacct cccacaacga ggactacacc atcgtggagc agtacgagcg
     3421 caccgagggc cgccaccacc tgttcctgta gcggccgcct cgactaggga taacagggta
     3481 attgtttgaa tgaggcttca gtactttaca gaatcgttgc ctgcacatct tggaaacact
     3541 tgctgggatt acttcgactt cttaacccaa cagaaggctc gagcaaccag aattcgagca
     3601 attatcttgt ttactaaaac tgaatacctt gctatctctt tgatacattt ttacaaagct
     3661 gaattaaaat ggtataaatt aaatcacttt tttcaattga cgcgtaattc taccgggtag
     3721 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
     3781 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
     3841 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
     3901 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
     3961 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
     4021 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
     4081 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
     4141 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
     4201 ctccgggcct ttcgacctgc agcccaagct tccaccatgg tgagcaaggg cgaggagctg
     4261 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     4321 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagctgatc
     4381 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gggctacggc
     4441 ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     4501 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     4561 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     4621 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     4681 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc
     4741 cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc
     4801 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg
     4861 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     4921 gggatcactc tcggcatgga cgagctgtac aagtaagaat tgttcaattc ctgatcatcc
     4981 tgcaggatgc atgtcgactt cgaaacgcgt ctggaacaat caacctctgg attacaaaat
     5041 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     5101 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     5161 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     5221 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     5281 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     5341 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     5401 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     5461 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     5521 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     5581 gatctccctt tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa
     5641 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     5701 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     5761 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     5821 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     5881 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     5941 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     6001 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     6061 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     6121 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     6181 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     6241 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     6301 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     6361 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
     6421 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     6481 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     6541 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     6601 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     6661 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     6721 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     6781 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     6841 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     6901 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     6961 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     7021 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     7081 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     7141 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     7201 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     7261 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     7321 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     7381 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     7441 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     7501 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     7561 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     7621 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     7681 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     7741 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     7801 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     7861 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     7921 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     7981 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     8041 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     8101 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     8161 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     8221 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     8281 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     8341 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     8401 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     8461 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     8521 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     8581 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     8641 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     8701 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     8761 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     8821 tggaaaaacg ccagcaacgc ggccttttta cggttcctg
//
