LOCUS       sequence-211523-        9438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Worseck JM, Grossmann A, Weimann M, Hegele A, Stelzl U
  TITLE     A stringent yeast two-hybrid matrix screening approach for 
            protein-protein interaction discovery.
  JOURNAL   Methods Mol Biol. 2012;812:63-87. doi: 10.1007/978-1-61779-455-1_4.
  PUBMED    22218854
REFERENCE   2  (bases 1 to 9438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2012"; volume: "812:63-87. doi"; pages: " 
            10.1007/978-1-61779-455-1_4"
COMMENT     Sequence Label: pACT4-DM
FEATURES             Location/Qualifiers
     source          1..9438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      38..1380
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(1001..1048)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        1645..1749
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1750..1818,1819..2610)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1968..1987)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2781..3369
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3270..3289
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3523..3540
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3555..3695
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3621..3640)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3781..3803
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        3832..4233
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             4279..4299
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4315..4656
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     protein_bind    4663..4787
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4812..4817,4818..4835,4836..4842)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4896..5555
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4962..4981)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5897..6202
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6081..6100
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6243..6367)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      6757..6944
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6812..6836)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    complement(6965..6998)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(7499..8593)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(8594..8999)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
ORIGIN
        1 gcgtttccat cttgcacttc aatagcatat ctttgttaac gaagcatctg tgcttcattt
       61 tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat
      121 ttttacagaa cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc
      181 atttttgtaa aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc
      241 tgcattttta cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat
      301 acttcttttt tgttctacaa aaatgcatcc cgagagcgct atttttctaa caaagcatct
      361 tagattactt tttttctcct ttgtgcgctc tataatgcag tctcttgata actttttgca
      421 ctgtaggtcc gttaaggtta gaagaaggct actttggtgt ctattttctc ttccataaaa
      481 aaagcctgac tccacttccc gcgtttactg attactagcg aagctgcggg tgcatttttt
      541 caagataaag gcatccccga ttatattcta taccgatgtg gattgcgcat actttgtgaa
      601 cagaaagtga tagcgttgat gattcttcat tggtcagaaa attatgaacg gtttcttcta
      661 ttttgtctct atatactacg tataggaaat gtttacattt tcgtattgtt ttcgattcac
      721 tctatgaata gttcttacta caattttttt gtctaaagag taatactaga gataaacata
      781 aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt
      841 atatagggat atagcacaga gatatatagc aaagagatac ttttgagcaa tgtttgtgga
      901 agcggtattc gcaatatttt agtagctcgt tacagtccgg tgcgtttttg gttttttgaa
      961 agtgcgtctt cagagcgctt ttggttttca aaagcgctct gaagttccta tactttctag
     1021 agaataggaa cttcggaata ggaacttcaa agcgtttccg aaaacgagcg cttccgaaaa
     1081 tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc gcacctatat ctgcgtgttg
     1141 cctgtatata tatatacatg agaagaacgg catagtgcgt gtttatgctt aaatgcgtac
     1201 ttatatgcgt ctatttatgt aggatgaaag gtagtctagt acctcctgtg atattatccc
     1261 attccatgcg gggtatcgta tgcttccttc agcactaccc tttagctgtt ctatatgctg
     1321 ccactcctca attggattag tctcatcctt caatgctatc atttcctttg atattggatc
     1381 atatgcatag taccgagaaa ctagtgcgaa gtagtgatca ggtattgctg ttatctgatg
     1441 agtatacgtt gtcctggcca cggcagaagc acgcttatcg ctccaatttc ccacaacatt
     1501 agtcaactcc gttaggccct tcattgaaag aaatgaggtc atcaaatgtc ttccaatgtg
     1561 agattttggg ccatttttta tagcaaagat tgaataaggc gcatttttct tcaaagctca
     1621 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     1681 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     1741 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     1801 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     1861 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     1921 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     1981 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     2041 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     2101 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     2161 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     2221 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     2281 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     2341 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     2401 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     2461 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     2521 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     2581 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     2641 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     2701 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     2761 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2821 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2881 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     2941 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3001 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3061 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3121 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3181 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3241 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3301 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3361 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3421 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3481 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     3541 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     3601 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     3661 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     3721 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     3781 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagcaac
     3841 ttcttttctt tttttttctt ttctctctcc cccgttgttg tctcaccata tccgcaatga
     3901 caaaaaaatg atggaagaca ctaaaggaaa aaattaacga caaagacagc accaacagat
     3961 gtcgttgttc cagagctgat gaggggtatc tcgaagcaca cgaaactttt tccttccttc
     4021 attcacgcac actactctct aatgagcaac ggtatacggc cttccttcca gttacttgaa
     4081 tttgaaataa aaaaaagttt gctgtcttgc tatcaagtat aaatagacct gcaattatta
     4141 atcttttgtt tcctcgtcat tgttctcgtt ccctttcttc cttgtttctt tttctgcaca
     4201 atatttcaag ctataccaag catacaatca actccaagct ttgcaaagat ggataaagcg
     4261 gaattaattc ccgagcctcc aaaaaagaag agaaaggtcg aattgggtac cgccgccaat
     4321 tttaatcaaa gtgggaatat tgctgatagc tcattgtcct tcactttcac taacagtagc
     4381 aacggtccga acctcataac aactcaaaca aattctcaag cgctttcaca accaattgcc
     4441 tcctctaacg ttcatgataa cttcatgaat aatgaaatca cggctagtaa aattgatgat
     4501 ggtaataatt caaaaccact gtcacctggt tggacggacc aaactgcgta taacgcgttt
     4561 ggaatcacta cagggatgtt taataccact acaatggatg atgtatataa ctatctattc
     4621 gatgatgaag ataccccacc aaacccaaaa aaagagatct ccacaagttt gtacaaaaaa
     4681 gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa
     4741 aaacagacta cataatactg taaaacacaa catatccagt cactatggcg gccgcattag
     4801 gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg
     4861 atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata
     4921 ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
     4981 ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
     5041 agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
     5101 ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
     5161 acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
     5221 accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg
     5281 aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc
     5341 cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc
     5401 ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga
     5461 ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac
     5521 aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg tggatccggc ttactaaaag
     5581 ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata
     5641 tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt
     5701 tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta
     5761 agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat
     5821 caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag
     5881 aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg
     5941 tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc
     6001 cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca
     6061 tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt
     6121 tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa
     6181 cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg
     6241 accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
     6301 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca
     6361 aagtggtaag taactaagat ctatgaatcg tagatactga aaaaccccgc aagttcactt
     6421 caactgtgca tcgtgcacca tctcaatttc tttcatttat acatcgtttt gccttctttt
     6481 atgtaactat actcctctaa gtttcaatct tggccatgta acctctgatc tatagaattt
     6541 tttaaatgac tagaattaat gcccatcttt tttttggacc taaattcttc atgaaaatat
     6601 attacgaggg cttattcaga agctttggac ttcttcgcca gaggtttggt caagtctcca
     6661 atcaaggttg tcggcttgtc taccttgcca gaaatttacg aaaagatgga aaagggtcaa
     6721 atcgttggta gatacgttgt tgacacttct aaataagcga atttcttatg atttatgatt
     6781 tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag
     6841 gttttaaaac gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct
     6901 caggtatagc atgaggtcgc tcttattgac cacacctcta ccggcatgcc ggtcgaggga
     6961 cctaataact tcgtatagca tacattatac gaagttatat taagggttcc ggatcgcggc
     7021 ctcgactacg tcgttaaggc cgtttctgac agagtaaaat tcttgaggga actttcacca
     7081 ttatgggaaa tggttcaaga aggtattgac ttaaactcca tcaaatggtc aggtcattga
     7141 gtgtttttta tttgttgtat tttttttttt ttagagaaaa tcctccaata tataaattag
     7201 gaatcatagt ttcatgattt tctgttacac ctaacttttt gtgtggtgcc ctcctccttg
     7261 tcaatattaa tgttaaagtg caattctttt tccttatcac gttgagccat tagtatcaat
     7321 ttgcttacct gtattccttt acatcctcct ttttctcctt cttgataaat gtatgtagat
     7381 tgcgtatata gtttcgtcta ccctatgaac atattccatt ttgtaatttc gtgtcgtttc
     7441 tattatgaat ttcatttata aagtttatgt acaaatatca taaaaaaaga gaatcttttt
     7501 aagcaaggat tttcttaact tcttcggcga cagcatcacc gacttcggtg gtactgttgg
     7561 aaccacctaa atcaccagtt ctgatacctg catccaaaac ctttttaact gcatcttcaa
     7621 tggccttacc ttcttcaggc aagttcaatg acaatttcaa catcattgca gcagacaaga
     7681 tagtggcgat agggttgacc ttattctttg gcaaatctgg agcagaaccg tggcatggtt
     7741 cgtacaaacc aaatgcggtg ttcttgtctg gcaaagaggc caaggacgca gatggcaaca
     7801 aacccaagga acctgggata acggaggctt catcggagat gatatcacca aacatgttgc
     7861 tggtgattat aataccattt aggtgggttg ggttcttaac taggatcatg gcggcagaat
     7921 caatcaattg atgttgaacc ttcaatgtag gaaattcgtt cttgatggtt tcctccacag
     7981 tttttctcca taatcttgaa gaggccaaaa cattagcttt atccaaggac caaataggca
     8041 atggtggctc atgttgtagg gccatgaaag cggccattct tgtgattctt tgcacttctg
     8101 gaacggtgta ttgttcacta tcccaagcga caccatcacc atcgtcttcc tttctcttac
     8161 caaagtaaat acctcccact aattctctga caacaacgaa gtcagtacct ttagcaaatt
     8221 gtggcttgat tggagataag tctaaaagag agtcggatgc aaagttacat ggtcttaagt
     8281 tggcgtacaa ttgaagttct ttacggattt ttagtaaacc ttgttcaggt ctaacactac
     8341 ctgtacccca tttaggacca cccacagcac ctaacaaaac ggcatcagcc ttcttggagg
     8401 cttccagcgc ctcatctgga agtggaacac ctgtagcatc gatagcagca ccaccaatta
     8461 aatgattttc gaaatcgaac ttgacattgg aacgaacatc agaaatagct ttaagaacct
     8521 taatggcttc ggctgtgatt tcttgaccaa cgtggtcacc tggcaaaacg acgatcttct
     8581 taggggcaga cattagaatg gtatatcctt gaaatatata tatatattgc tgaaatgtaa
     8641 aaggtaagaa aagttagaaa gtaagacgat tgctaaccac ctattggaaa aaacaatagg
     8701 tccttaaata atattgtcaa cttcaagtat tgtgatgcaa gcatttagtc atgaacgctt
     8761 ctctattcta tatgaaaagc cggttccggc gctctcacct ttcctttttc tcccaatttt
     8821 tcagttgaaa aaggtatatg cgtcaggcga cctctgaaat taacaaaaaa tttccagtca
     8881 tcgaatttga ttctgtgcga tagcgcccct gtgtgttctc gttatgttga ggaaaaaaat
     8941 aatggttgct aagagattcg aactcttgca tcttacgata cctgagtatt cccacagttc
     9001 cttaccactc ttttgttact ctattgatcc agctcagcaa aggcagtgtg atctaagatt
     9061 ctatcttcgc gatgtagtaa aactagctag accgagaaag agactagaaa tgcaaaaggc
     9121 acttctacaa tggctgccat cattattatc cgatgtgacg ctgcagcttc tcaatgatat
     9181 tcgaatacgc tttgaggaga tacagcctaa tatccgacaa actgttttac agatttacga
     9241 tcgtacttgt tacccatcat tgaattttga acatccgaac ctgggagttt tccctgaaac
     9301 agatagtata tttgaacctg tataataata tatagtctag cgctttacgg aagacaatgt
     9361 atgtatttcg gttcctggag aaactattgc atctattgca taggtaatct tgcacgtcgc
     9421 atccccggtt cattttct
//
