LOCUS       sequence-211524-       12210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  the overproduction of the universal building blocks to make 
            isoprenoids (i.e. IPP and DMAPP)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12210)
  AUTHORS   Alonso-Gutierrez J, Chan R, Batth TS, Adams PD, Keasling JD, Petzold
            CJ, Lee TS
  TITLE     Metabolic engineering of Escherichia coli for limonene and perillyl 
            alcohol production.
  JOURNAL   Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004.
  PUBMED    23727191
REFERENCE   2  (bases 1 to 12210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2013-05-29"; volume: "19C:33-41. doi"; pages: " 
            10.1016/j.ymben.2013.05.004"
COMMENT     Sequence Label: pBbA5c-MevT-MBI (JBEI-3085)
FEATURES             Location/Qualifiers
     source          1..12210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2393..2404
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             6011..6022
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      7535..7606
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7622..7649
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(7677..7694)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7811..8356)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(7891..7910)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(8470..8564)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(8585..9244)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     9159..9178
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(9245..9347)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             complement(9419..10501)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     10463..10482
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(10502..10579)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     10785..10804
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(10890..10895,10896..10913,10914..10920)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    10928..10944
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             complement(11679..11690)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tttgttggtg tggtattaaa ggaagactta ggccgcaaaa gcaacaacaa ttacacaata
       61 caaacttgca aatgactgaa ctaaaaaaac aaaagaccgc tgaacaaaaa accagacctc
      121 aaaatgtcgg tattaaaggt atccaaattt acatcccaac tcaatgtgtc aaccaatctg
      181 agctagagaa atttgatggc gtttctcaag gtaaatacac aattggtctg ggccaaacca
      241 acatgtcttt tgtcaatgac agagaagata tctactcgat gtccctaact gttttgtcta
      301 agttgatcaa gagttacaac atcgacacca acaaaattgg tagattagaa gtcggtactg
      361 aaactctgat tgacaagtcc aagtctgtca agtctgtctt gatgcaattg tttggtgaaa
      421 acactgacgt cgaaggtatt gacacgctta atgcctgtta cggtggtacc aacgcgttgt
      481 tcaactcttt gaactggatt gaatctaacg catgggatgg tagagacgcc attgtagttt
      541 gcggtgatat tgccatctac gataagggtg ccgcaagacc aaccggtggt gccggtactg
      601 ttgctatgtg gatcggtcct gatgctccaa ttgtatttga ctctgtaaga gcttcttaca
      661 tggaacacgc ctacgatttt tacaagccag atttcaccag cgaatatcct tacgtcgatg
      721 gtcatttttc attaacttgt tacgtcaagg ctcttgatca agtttacaag agttattcca
      781 agaaggctat ttctaaaggg ttggttagcg atcccgctgg ttcggatgct ttgaacgttt
      841 tgaaatattt cgactacaac gttttccatg ttccaacctg taaattggtc acaaaatcat
      901 acggtagatt actatataac gatttcagag ccaatcctca attgttccca gaagttgacg
      961 ccgaattagc tactcgcgat tatgacgaat ctttaaccga taagaacatt gaaaaaactt
     1021 ttgttaatgt tgctaagcca ttccacaaag agagagttgc ccaatctttg attgttccaa
     1081 caaacacagg taacatgtac accgcatctg tttatgccgc ctttgcatct ctattaaact
     1141 atgttggatc tgacgactta caaggcaagc gtgttggttt attttcttac ggttccggtt
     1201 tagctgcatc tctatattct tgcaaaattg ttggtgacgt ccaacatatt atcaaggaat
     1261 tagatattac taacaaatta gccaagagaa tcaccgaaac tccaaaggat tacgaagctg
     1321 ccatcgaatt gagagaaaat gcccatttga agaagaactt caaacctcaa ggttccattg
     1381 agcatttgca aagtggtgtt tactacttga ccaacatcga tgacaaattt agaagatcct
     1441 acgatgttaa aaaatgagga tctaaaataa ggaggattac actatggttt taaccaataa
     1501 aacagtcatt tctggatcga aagtcaaaag tttatcatct gcgcaatcga gctcatcagg
     1561 accttcatca tctagtgagg aagatgattc ccgcgatatt gaaagcttgg ataagaaaat
     1621 acgtccttta gaagaattag aagcattatt aagtagtgga aatacaaaac aattgaagaa
     1681 caaagaggtc gctgccttgg ttattcacgg taagttacct ttgtacgctt tggagaaaaa
     1741 attaggtgat actacgagag cggttgcggt acgtaggaag gctctttcaa ttttggcaga
     1801 agctcctgta ttagcatctg atcgtttacc atataaaaat tatgactacg accgcgtatt
     1861 tggcgcttgt tgtgaaaatg ttataggtta catgcctttg cccgttggtg ttataggccc
     1921 cttggttatc gatggtacat cttatcatat accaatggca actacagagg gttgtttggt
     1981 agcttctgcc atgcgtggct gtaaggcaat caatgctggc ggtggtgcaa caactgtttt
     2041 aactaaggat ggtatgacaa gaggcccagt agtccgtttc ccaactttga aaagatccgg
     2101 tgcctgtaag atatggttag actcagaaga gggacaaaac gcaattaaaa aagcttttaa
     2161 ctctacatca agatttgcac gtctgcaaca tattcaaact tgtctagcag gagatttact
     2221 cttcatgaga tttagaacaa ctactggtga cgcaatgggt atgaatatga tttctaaagg
     2281 tgtcgaatac tcattaaagc aaatggtaga agagtatggc tgggaagata tggaggttgt
     2341 ctccgtttct ggtaactact gtaccgacaa aaaaccagct gccatcaact ggatcgaagg
     2401 tcgtggtaag agtgtcgtcg cagaagctac tattcctggt gatgttgtca gaaaagtgtt
     2461 aaaaagtgat gtttccgcat tggttgagtt gaacattgct aagaatttgg ttggatctgc
     2521 aatggctggg tctgttggtg gatttaacgc acatgcagct aatttagtga cagctgtttt
     2581 cttggcatta ggacaagatc ctgcacaaaa tgttgaaagt tccaactgta taacattgat
     2641 gaaagaagtg gacggtgatt tgagaatttc cgtatccatg ccatccatcg aagtaggtac
     2701 catcggtggt ggtactgttc tagaaccaca aggtgccatg ttggacttat taggtgtaag
     2761 aggcccgcat gctaccgctc ctggtaccaa cgcacgtcaa ttagcaagaa tagttgcctg
     2821 tgccgtcttg gcaggtgaat tatccttatg tgctgcccta gcagccggcc atttggttca
     2881 aagtcatatg acccacaaca ggaaacctgc tgaaccaaca aaacctaaca atttggacgc
     2941 cactgatata aatcgtttga aagatgggtc cgtcacctgc attaaatcct gaggatctag
     3001 gaggttaatt ggatgtcatt accgttctta acttctgcac cgggaaaggt tattattttt
     3061 ggtgaacact ctgctgtgta caacaagcct gccgtcgctg ctagtgtgtc tgcgttgaga
     3121 acctacctgc taataagcga gtcatctgca ccagatacta ttgaattgga cttcccggac
     3181 attagcttta atcataagtg gtccatcaat gatttcaatg ccatcaccga ggatcaagta
     3241 aactcccaaa aattggccaa ggctcaacaa gccaccgatg gcttgtctca ggaactcgtt
     3301 agtcttttgg acccgttgtt agctcaacta tccgaatcct tccactacca tgcagcgttt
     3361 tgtttcctgt atatgtttgt ttgcctatgc ccccatgcca agaatattaa gttttcttta
     3421 aagtctactt tacccatcgg tgctgggttg ggctcaagcg cctctatttc tgtatcactg
     3481 gccttagcta tggcctactt gggggggtta ataggatcta atgacttgga aaagctgtca
     3541 gaaaacgata agcatatagt gaatcaatgg gccttcatag gtgaaaagtg tattcacggt
     3601 accccttcag gaatagataa cgctgtggcc acttatggta atgccctgct atttgaaaaa
     3661 gactcacata atggaacaat aaacacaaac aattttaagt tcttagatga tttcccagcc
     3721 attccaatga tcctaaccta tactaggatt ccaaggtcta caaaagacct tgttgctcgc
     3781 gttcgtgtgt tggtcaccga gaaatttcct gaagttatga agccaattct agatgccatg
     3841 ggtgaatgtg ccctacaagg cttagagatc atgactaagt taagtaaatg taaaggcacc
     3901 gatgacgagg ctgtagaaac taataatgaa ctgtatgaac aactattgga attgataaga
     3961 ataaatcatg gactgcttgt ctcaatcggt gtttctcatc ctggattaga acttattaaa
     4021 aatctgagcg atgatttgag aattggctcc acaaaactta ccggtgctgg tggcggcggt
     4081 tgctctttga ctttgttacg aagagacatt actcaagagc aaattgacag cttcaaaaag
     4141 aaattgcaag atgattttag ttacgagaca tttgaaacag acttgggtgg gactggctgc
     4201 tgtttgttaa gcgcaaaaaa tttgaataaa gaccttaaaa tcaaatccct agtattccaa
     4261 ttatttgaaa ataaaactac cacaaagcaa caaattgacg atctattatt gccaggaaac
     4321 acgaatttac catggacttc atagggatct taaggaggat accctatgtc agagttgaga
     4381 gccttcagtg ccccagggaa agcgttacta gctggtggat atttagtttt agatacaaaa
     4441 tatgaagcat ttgtagtcgg attatcggca agaatgcatg ctgtagccca tccttacggt
     4501 tcattgcaag ggtctgataa gtttgaagtg cgtgtgaaaa gtaaacaatt taaagatggg
     4561 gagtggctgt accatataag tcctaaaagt ggcttcattc ctgtttcgat aggcggatct
     4621 aagaaccctt tcattgaaaa agttatcgct aacgtattta gctactttaa acctaacatg
     4681 gacgactact gcaatagaaa cttgttcgtt attgatattt tctctgatga tgcctaccat
     4741 tctcaggagg atagcgttac cgaacatcgt ggcaacagaa gattgagttt tcattcgcac
     4801 agaattgaag aagttcccaa aacagggctg ggctcctcgg caggtttagt cacagtttta
     4861 actacagctt tggcctcctt ttttgtatcg gacctggaaa ataatgtaga caaatataga
     4921 gaagttattc ataatttagc acaagttgct cattgtcaag ctcagggtaa aattggaagc
     4981 gggtttgatg tagcggcggc agcatatgga tctatcagat atagaagatt cccacccgca
     5041 ttaatctcta atttgccaga tattggaagt gctacttacg gcagtaaact ggcgcatttg
     5101 gttgatgaag aagactggaa tattacgatt aaaagtaacc atttaccttc gggattaact
     5161 ttatggatgg gcgatattaa gaatggttca gaaacagtaa aactggtcca gaaggtaaaa
     5221 aattggtatg attcgcatat gccagaaagc ttgaaaatat atacagaact cgatcatgca
     5281 aattctagat ttatggatgg actatctaaa ctagatcgct tacacgagac tcatgacgat
     5341 tacagcgatc agatatttga gtctcttgag aggaatgact gtacctgtca aaagtatcct
     5401 gaaatcacag aagttagaga tgcagttgcc acaattagac gttcctttag aaaaataact
     5461 aaagaatctg gtgccgatat cgaacctccc gtacaaacta gcttattgga tgattgccag
     5521 accttaaaag gagttcttac ttgcttaata cctggtgctg gtggttatga cgccattgca
     5581 gtgattacta agcaagatgt tgatcttagg gctcaaaccg ctaatgacaa aagattttct
     5641 aaggttcaat ggctggatgt aactcaggct gactggggtg ttaggaaaga aaaagatccg
     5701 gaaacttatc ttgataaata gggatctagg aggattatga gatgaccgtt tacacagcat
     5761 ccgttaccgc acccgtcaac atcgcaaccc ttaagtattg ggggaaaagg gacacgaagt
     5821 tgaatctgcc caccaattcg tccatatcag tgactttatc gcaagatgac ctcagaacgt
     5881 tgacctctgc ggctactgca cctgagtttg aacgcgacac tttgtggtta aatggagaac
     5941 cacacagcat cgacaatgaa agaactcaaa attgtctgcg cgacctacgc caattaagaa
     6001 aggaaatgga atcgaaggac gcctcattgc ccacattatc tcaatggaaa ctccacattg
     6061 tctccgaaaa taactttcct acagcagctg gtttagcttc ctccgctgct ggctttgctg
     6121 cattggtctc tgcaattgct aagttatacc aattaccaca gtcaacttca gaaatatcta
     6181 gaatagcaag aaaggggtct ggttcagctt gtagatcgtt gtttggcgga tacgtggcct
     6241 gggaaatggg aaaagctgaa gatggtcatg attccatggc agtacaaatc gcagacagct
     6301 ctgactggcc tcagatgaaa gcttgtgtcc tagttgtcag cgatattaaa aaggatgtga
     6361 gttccactca gggtatgcaa ttgaccgtgg caacctccga actatttaaa gaaagaattg
     6421 aacatgtcgt accaaagaga tttgaagtca tgcgtaaagc cattgttgaa aaagatttcg
     6481 ccacctttgc aaaggaaaca atgatggatt ccaactcttt ccatgccaca tgtttggact
     6541 ctttccctcc aatattctac atgaatgaca cttccaagcg tatcatcagt tggtgccaca
     6601 ccattaatca gttttacgga gaaacaatcg ttgcatacac gtttgatgca ggtccaaatg
     6661 ctgtgttgta ctacttagct gaaaatgagt cgaaactctt tgcatttatc tataaattgt
     6721 ttggctctgt tcctggatgg gacaagaaat ttactactga gcagcttgag gctttcaacc
     6781 atcaatttga atcatctaac tttactgcac gtgaattgga tcttgagttg caaaaggatg
     6841 ttgccagagt gattttaact caagtcggtt caggcccaca agaaacaaac gaatctttga
     6901 ttgacgcaaa gactggtcta ccaaaggaat aaggatctag gaggtaatga taatgcaaac
     6961 ggaacacgtc attttattga atgcacaggg agttcccacg ggtacgctgg aaaagtatgc
     7021 cgcacacacg gcagacaccc gcttacatct cgcgttctcc agttggctgt ttaatgccaa
     7081 aggacaatta ttagttaccc gccgcgcact gagcaaaaaa gcatggcctg gcgtgtggac
     7141 taactcggtt tgtgggcacc cacaactggg agaaagcaac gaagacgcag tgatccgccg
     7201 ttgccgttat gagcttggcg tggaaattac gcctcctgaa tctatctatc ctgactttcg
     7261 ctaccgcgcc accgatccga gtggcattgt ggaaaatgaa gtgtgtccgg tatttgccgc
     7321 acgcaccact agtgcgttac agatcaatga tgatgaagtg atggattatc aatggtgtga
     7381 tttagcagat gtattacacg gtattgatgc cacgccgtgg gcgttcagtc cgtggatggt
     7441 gatgcaggcg acaaatcgcg aagccagaaa acgattatct gcatttaccc agcttaaata
     7501 aggatccaag cctcgagtaa ggatctccag gcatcaaata aaacgaaagg ctcagtcgaa
     7561 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     7621 tggctcacct tcgggtgggc ctttctgcgt ttatacctag ggatatattc cgcttcctcg
     7681 ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg
     7741 ggcggagatt tcctggaaga tgccaggaag atacttaaca gggaagtgag agggccgcgg
     7801 caaagccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa
     7861 atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggcggct
     7921 ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc
     7981 cgcgtttgtc tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg
     8041 gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt
     8101 cttgagtcca acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga
     8161 tttagaggag ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt
     8221 tggtgactgc gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac
     8281 cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca gagcaagaga ttacgcgcag
     8341 accaaaacga tctcaagaag atcatcttat taatcagata aaatatttct agatttcagt
     8401 gcaatttatc tcttcaaatg tagcacctga agtcagcccc atacgatata agttgttact
     8461 agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
     8521 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat
     8581 caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
     8641 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
     8701 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     8761 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
     8821 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     8881 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     8941 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     9001 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa
     9061 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     9121 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     9181 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     9241 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
     9301 atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattttc
     9361 gccagatatc gacgtcggtg cctaatgagt gagctaactt acattaattg cgttgcgctc
     9421 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     9481 cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga
     9541 cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca
     9601 cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac
     9661 atgagctgtc ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc
     9721 cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg
     9781 cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac
     9841 tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc
     9901 agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt
     9961 gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga
    10021 aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag
    10081 tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc
    10141 cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc
    10201 gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg
    10261 cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg
    10321 actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg
    10381 ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg
    10441 aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca
    10501 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
    10561 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
    10621 aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc
    10681 aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa
    10741 caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
    10801 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
    10861 aggatcgaga tcgtttaggc accccaggct ttacacttta tgcttccggc tcgtataatg
    10921 tgtggaattg tgagcggata acaatttcag aattcaaaag atcttaggag gaatataaaa
    10981 tgaaaaattg tgtcatcgtc agtgcggtac gtactgctat cggtagtttt aacggttcac
    11041 tcgcttccac cagcgccatc gacctggggg cgacagtaat taaagccgcc attgaacgtg
    11101 caaaaatcga ttcacaacac gttgatgaag tgattatggg taacgtgtta caagccgggc
    11161 tggggcaaaa tccggcgcgt caggcactgt taaaaagcgg gctggcagaa acggtgtgcg
    11221 gattcacggt caataaagta tgtggttcgg gtcttaaaag tgtggcgctt gccgcccagg
    11281 ccattcaggc aggtcaggcg cagagcattg tggcgggggg tatggaaaat atgagtttag
    11341 ccccctactt actcgatgca aaagcacgct ctggttatcg tcttggagac ggacaggttt
    11401 atgacgtaat cctgcgcgat ggcctgatgt gcgccaccca tggttatcat atggggatta
    11461 ccgccgaaaa cgtggctaaa gagtacggaa ttacccgtga aatgcaggat gaactggcgc
    11521 tacattcaca gcgtaaagcg gcagccgcaa ttgagtccgg tgcttttaca gccgaaatcg
    11581 tcccggtaaa tgttgtcact cgaaagaaaa ccttcgtctt cagtcaagac gagttcccga
    11641 aagcgaactc aacggctgaa gcgttaggtg cattgcgccc ggccttcgat aaagcaggaa
    11701 cagtcaccgc tgggaacgcg tctggtatta acgacggtgc tgccgctctg gtgattatgg
    11761 aagaatctgc ggcgctggca gcaggcctta cccccctggc tcgcattaaa agttatgcca
    11821 gcggtggcgt gccccccgca ttgatgggta tggggccagt acctgccacg caaaaagcgt
    11881 tacaactggc ggggctgcaa ctggcggata ttgatctcat tgaggctaat gaagcatttg
    11941 ctgcacagtt ccttgccgtt gggaaaaacc tgggctttga ttctgagaaa gtgaatgtca
    12001 acggcggggc catcgcgctc gggcatccta tcggtgccag tggtgctcgt attctggtca
    12061 cactattaca tgccatgcag gcacgcgata aaacgctggg gctggcaaca ctgtgcattg
    12121 gcggcggtca gggaattgcg atggtgattg aacggttgaa ttgaggatct tgaattaagg
    12181 aggacagcta aatgaaactc tcaactaaac
//
