LOCUS       sequence-211528-       13311 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To insert a single copy of a construct at a site on Chr II, 
            corresponding to mKate expressed specifically in the epidermis; can 
            be used for N- or C-terminal fusion..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13311)
  TITLE     Ewbank lab plasmids
REFERENCE   2  (bases 1 to 13311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pNP172
FEATURES             Location/Qualifiers
     source          1..13311
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     145..162
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     146..162
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2857..2937
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     terminator      2946..3269
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     4559..4581
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4619..4637)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4705..4809
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4810..4878,4879..5670)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5028..5047)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5841..6429
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6330..6349
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(7917..7950)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             complement(7950..7961)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(9383..9706)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    11624..11648
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             11651..12676
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             11651..12676
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(12677..12701)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(13054..13087)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
ORIGIN
        1 aaaaaaaaat gtttagattt ggattgaatt aattttttag aaagcaagaa aaaaaacaca
       61 aagagaataa ataaataata ggaaaaaaaa caaatattca gtatttaaaa tttgaaaatt
      121 caacgacgtt ggcgtcgatc atcctgtaaa acgacggcca gtgctgcatt ttcagtattt
      181 gctattgaca aataaaattt ccaattatta ttcacttaaa cttctcgttt acacaaaatc
      241 aaaagcgatg taattgttcc tgattagttc actcaaaact tttttttcac ctactgttta
      301 tctccttctg ttctttttct ttattccctc taattcatcc attctgtttt aattcacact
      361 gcgcgccgca acaccatgtc gctgtaatat ttagtcagtt tttgagaaaa taggtataga
      421 gacagagacg ctgacaagac aacactaggt ataaaagcgg aagtggtggc tttaaaatca
      481 cttggcttct aaagtccagt gacaggtaag gttctcgtta cttccgtctc gattactaag
      541 atttgattac tttttagaaa gatggtctcc gagctcatta aagaaaacat gcatatgaag
      601 ctctacatgg agggaaccgt caacaaccac cacttcaagt gcacctccga gggagaggga
      661 aagccatacg agggaaccca aaccatgcgt atcaaggccg tcgagggagg accactccca
      721 ttcgccttcg acatcctcgc cacctccttc atgtacggat ccaaggtaag tttaaacata
      781 tatatactaa ctaaccctga ttatttaaat tttcagacct tcatcaacca cacccaagga
      841 atcccagact tcttcaagca atccttccca gagggattca cctgggagcg tgtcaccacc
      901 tacgaggacg gaggagtcct caccgccacc caagacacct ccctccaaga cggatgcctc
      961 atctacaacg tcaaggtaag tttaaacagt tcggtactaa ctaaccatac atatttaaat
     1021 tttcagatcc gtggagtcaa cttcccatcc aacggaccag tcatgcaaaa gaagaccctc
     1081 ggatgggagg cctccaccga gaccctctac ccagccgacg gaggacttga gggacgtgcc
     1141 gacatggccc tcaagctcgt cggaggagga cacctcatct gcaacctcaa ggtaagttta
     1201 aacatgattt tactaactaa ctaatctgat ttaaattttc agaccaccta ccgttccaag
     1261 aagccagcca agaacctcaa gatgccagga gtctactacg tcgaccgtcg tcttgagcgt
     1321 atcaaggagg ccgacaagga gacctacgtc gagcaacacg aggtcgccgt cgcccgttac
     1381 tgcgacctcc catccaagct cggacaccgt ggtggaggag gatctggagg aggaggatcc
     1441 atggtttccg agttgatcaa ggagaatatg cacatgaaac tctacatgga aggaaccgtc
     1501 aacaatcatc atttcaaatg tacttcagaa ggagaaggaa aaccatacga gggaactcag
     1561 accatgagaa tcaaggctgt tgagggaggt aagtttaaaa aatacgtgtt cataacttga
     1621 tacgaatata aattttcagg accacttcca ttcgcgttcg acattcttgc cacttcgttc
     1681 atgtatggaa gcaagacttt cattaatcac acacaaggga tcccggattt cttcaagcaa
     1741 tctttcccag aaggattcac atgggagaga gttaccactt acgaggatgg aggagttctt
     1801 accgccaccc aagacacatc cctccaaggt aagtttaaaa gattatagca atgttcgtct
     1861 aaatataata aattttcaga tggatgtctg atctacaacg tcaagatcag aggagtcaat
     1921 ttcccatcaa atggaccagt tatgcagaag aagactttgg gctgggaggc ttccaccgag
     1981 accctttacc cagcagatgg aggtctcgag ggccgcgctg atatggctct caaactcgtc
     2041 ggaggaggac acttgatctg caatttaaaa accacctacc ggtcgaaggt aagtttaaaa
     2101 tcaattttta atagcttata taataataca aattttcaga agccagccaa gaatctcaaa
     2161 atgccaggag tttactatgt tgaccgtcgt cttgagcgca ttaaagaggc cgataaggaa
     2221 acgtacgttg aacaacatga agttgccgtt gctcgatatt gtgatctccc atccaagctc
     2281 ggacaccgtg ccggccacgg actccaagac gacccagacc tccaagccct cctcaaggga
     2341 tcccaactcc tcaaggtcaa gtcctcctcc tggcgtcgtg agcgtttcta caagctccaa
     2401 gaggactgca aggtaagttt aaacatatat atactaacta accctgatta tttaaatttt
     2461 cagaccatct ggcaagagtc ccgtaaggta agtttaaaca gttcggtact aactaaccat
     2521 acatatttaa attttcaggt catgcgttcc ccagagtccc aactcttctc catcgaggac
     2581 atccaagagg tccgtatggg acaccgtacc gagggactcg agaagttcgc ccgtgacatc
     2641 ccagaggacc gttgcttctc catcgtcttc aaggtaagtt taaacatgat tttactaact
     2701 aactaatctg atttaaattt tcaggaccaa cgtaacaccc tcgacctcat cgccccatcc
     2761 ccagccgacg cccaacactg ggtccaagga ctccgtaaga tcatccacca ctccggatcc
     2821 atggaccaac gtcaaaagtc aggagctagc ggagcctacc cttacgacgt gccagattac
     2881 gcttacccct acgacgtacc agactacgcc tacccatacg acgtcccaga ctacgcttaa
     2941 gataaatgca aaatcctttc aagcattccc ttcttctcta tcactcttct ttctttttgt
     3001 caaaaaattc tctcgctaat ttatttgctt ttttaatgtt attattttat gactttttat
     3061 agtcactgaa aagtttgcat ctgagtgaag tgaatgctat caaaatgtga ttctgtctga
     3121 tgtactttca caatctctct tcaattccat tttgaagtgc tttaaacccg aaaggttgag
     3181 aaaaatgcga gcgctcaaat atttgtattg tgttcgttga gtgacccaac aaaaagagga
     3241 aactttattg tgccgccaag aaaaaagtct cacgtacggt gcgcgcgatg ccgtatcaca
     3301 actgagaaaa aagacaaaac ttattgaaca gtgttccttt caaaaaatta ttttgtaaaa
     3361 aatcgagttt ttctccaaaa taacgcacgc aatcaaaaac aagaaaacta acgtgttctc
     3421 ctgggcgtgg aactccttat caagattacc attattgtct gtcagaattg atatttcaat
     3481 tatttcatat ctagacaaac ccgggtgtct tgcatttatt agtactgtga gtttacatac
     3541 aaacgaggct tactcagata acatagagtc acctctaatt agcaaaaacc catttctaat
     3601 tagctatttc gaccagttct aaagtcaccg taattagaaa tggtttttgt agcttttcta
     3661 attagaaatg accccgagat aagtgaaata tggcattttc tgggttttct agtaagaaat
     3721 gatcccgcac gtagtgattc cgcgttacaa gccaaattca aattaaaaaa gtatattaca
     3781 caattcaagt tacattattt tcaggaaaca atggcgaaga gcaaagcaga gattcaaaag
     3841 gaggaaagag acaacagata tgaacgaaga cttcttgaaa agagaaagag acatgaggtg
     3901 agtaaccatg ataataaata atcaagtaag ggctatagat ttataaattc tggagttaac
     3961 agtttcgaaa aaaagtagtt ttcatatgtg cgaaggaata tcaaaaatag gggaatagcc
     4021 taaacgagtg agaaaattaa atgtttgtta ttttttcaaa aatgtctgca gggccgttat
     4081 cctttcaaat gcactctatc aaacatagat catatccatc atatcttatc cagaatttat
     4141 atcatatcca agattgagag cgtttcaagg aaaatctcaa acaaccatac aattttgtag
     4201 atcgacaaca aggcctccac ttcttccacg tgacgtcgtg actgggaaaa ccctggcgtt
     4261 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     4321 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
     4381 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     4441 gcaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     4501 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     4561 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     4621 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     4681 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4741 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     4801 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     4861 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     4921 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     4981 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5041 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     5101 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5161 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5221 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5281 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5341 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     5401 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     5461 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     5521 cgtgggtctc gcggtatcat tgcagcactg gggtcagatg gtaagccctc ccgtatcgta
     5581 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     5641 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     5701 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5761 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5821 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5881 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5941 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     6001 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6061 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6121 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6181 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6241 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6301 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6361 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6421 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggtcttttg ctggcctttt
     6481 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     6541 gagtgagctg ataccagcgg ataacaattt cacagggccc tgtcggatta tgggctcttc
     6601 ttttatcctc tactttcttt ctagaaaatg caaatgttga gtcagaaatt gctgaatgga
     6661 gtacaactgc aatgggttta gcttatgttt ctggaacaat tactggtgga attgtgattg
     6721 aaaagtaagg ttatatttca tttcaataaa tgttaccaca gtataacaaa aacaagttac
     6781 attgttcaga gtatgtctga atgtattatt attattgtat ttgtgcatca gtactgtagt
     6841 tgtactgaag aagggtttaa aataatctca acgtttttaa gacgagttag tgagaatacg
     6901 gtcgcagtta ggatgtttgg acatagtttt attagataat cgtgaactaa ctcactataa
     6961 aaacccgcta actcattgaa taagatagtt ttaaacgttc aaaatttgaa gacaaaattg
     7021 tatgaataag tttacagagt tggtcgacgc aagcttctcc ttctcttcac atttctcaat
     7081 aatttagctc taattgcatt cgtattcttc gcaaaaatac gtattctgat tgacccgatg
     7141 aaatacggat gcctagttgc attgatcatc tacggttata cttatgggtg aggtttttca
     7201 aatatatttt gatttaatcg aataatgaaa tttcagaact ggagttggtc caatttcgtg
     7261 gtttatatcc tctgaactgg tacctcaaaa acatcgtagt attgctcaat ctgtggcata
     7321 ttcaatgaat actgtaatgg tggttatcag tacatttaca gtattacctc tatattccgt
     7381 tattggtagt tatgctttcc ttattctata ttcaattcct tcatttataa gtatgcttat
     7441 attatttcgc tacttaccgg aaaccaaagg acgagagatt catgatattg tgaatgaact
     7501 taaaaaaaaa tgaaaacctt tgaaaacgtt catacggccg aaattttact tgatcgagcc
     7561 aattgaactt ttgttaagac ttacagaaaa gtgagagatt ttgttacaaa aaagtgagaa
     7621 aaaagcgaaa atgaacagga aaaagaagaa aaatacaccc gaagtatcat ataaaaatta
     7681 aaaagattgt ttgacctggc ggaacttttt tgcaaaaact ctgaatatcc atggcacatg
     7741 cttcgtgcaa aacaggatcc aaatgaatat caacaagaga ttcttgaaga cgacgagcca
     7801 cttgctcaga gcattgctta tctcgaatga gacccttgtt aaaatcattt ttcaaacatt
     7861 atatgacatt gtcgaaatgt cctcctgatt ccatgatggt agcaaactca cttcgtataa
     7921 cttcgtatag tataccttat acgaagttat ctgccctcga ttgtctatct tccattctca
     7981 ccaagtttca acaactttat tcagtcattc ttctctggtt catttcccaa ccaatcttcc
     8041 cgaataaagc ccatctgtcc tttcggccac attcctcacc tacatccgta ctccttatct
     8101 cccgttctta tccagtcttt tatcgtctct gcgtctcgca cactgtctcg tcggtaatgt
     8161 aaaaaaaaaa caaaaaaaac ttgaaaactt gcggaaagat ttcctaataa aaggagacga
     8221 atccggtagg aaatcgacaa gtctctgctt ctcgaagact aggtgagtta catttctttg
     8281 gccgaattat tttgaaatcc taaatttgat aatttcagag atgtctgtaa aacttgcgtg
     8341 ttatgtgacg gcttcggtca ctgtcgccac tcttatggta tgcttcatga ccatgtcaac
     8401 catctactcg gaggttgatg gattcagaga gaagcttgat accgagatga atgtgttcag
     8461 agtgagttaa attatatttt gaattttaaa taatttttaa ttttctagca atctaccaat
     8521 ggattgtgga aggacatagt tgtcatcgga agatctagca agcgtgtccg ttgtcaatat
     8581 gaagagacca acgctacccc aactccacat gctgatggat ccccatctgc tccaccaggt
     8641 caaccaccag cagttccacc agtcttcaac cagccaaaga ctccaaatgg agccaatgga
     8701 aatggaccaa cctgcaactg caatgctgat aacaagtgcc cagctggacc atccggacca
     8761 aagggagttc caggagttcc aggactcgac ggagttccag gacttgacgg tgttccagga
     8821 gttggagctg atgatatcgc tccacaacgc gagtctgtcg gatgcttcac ttgcccacaa
     8881 ggaccagttg gaccaccagg agctcttgga agaccaggac cacgtggact tccaggacca
     8941 agaggacaaa atggaaaccc aggaagagat ggacaaccag gacatccagg agagcaagga
     9001 tcatccggtc aaatcggaaa gatcggagag ccaggaccac caggagagaa gggacgcgac
     9061 gccgagcatc caatcggaag accaggacca aagggaccaa gaggagatca aggaccaaca
     9121 ggaccagctg gacagaacgg tcttcacgga ccaccaggag agccaggaac cgttggacca
     9181 gaaggaccat ctggaaagca aggacgtcaa ggaccagacg gaacccaggg agagactgga
     9241 ccagacggaa gaccaggaaa ggatgccgag tactgccagt gcccagacaa gtctccacca
     9301 tcagaggctg tcaacgccaa ccgtggatac agaaatatct aaattgttgg tgttttctaa
     9361 taaaatattt gagattcagt gagacttttt tcttggcggc acaataaagt ttcctctttt
     9421 tgttgggtca ctcaacgaac acaatacaaa tatttgagcg ctcgcatttt tctcaacctt
     9481 tcgggtttaa agcacttcaa aatggaattg aagagagatt gtgaaagtac atcagacaga
     9541 atcacatttt gatagcattc acttcactca gatgcaaact tttcagtgac tataaaaagt
     9601 cataaaataa taacattaaa aaagcaaata aattagcgag agaatttttt gacaaaaaga
     9661 aagaatagtg atagagaaga agggaatgct tgaaaggatt ttgcatttat cttaatcacc
     9721 gtcttcgaga agacgaacca tggcgccagt ttcagaatcc aagtttctaa tatagttcat
     9781 cacaatgttg acgttggtcc aacctccggc ttgcataatc tctgggatgg aaactccagc
     9841 gcgagccatg tcacgggcag ctccgacacg ggcggaatgt ccagaccaag ccaagtaacg
     9901 ttgtccggaa tcatccttgg ctccgtagat gagacggtgg gtagcttcga agattcctga
     9961 aaatttattt tcagcttatt cttagctaat atgttgttta aacttacctt caagagctcg
    10021 agtcgagagt tgtgatgttg cactcggggc agcaactccg ttcttgcgaa cacgacagaa
    10081 caggtagttg tttggatcat cggcgactcc ggaaaccgaa atccagcgct caacgagctt
    10141 ggtaactcca agggagagcg ctttttcgac tccggcagtc gagaccagcg tttttgtacg
    10201 tccgatgtgg atgagcattc ttcctccgtc tgtgcgacta atatccttga cgcgaatgcg
    10261 ggcaatttcg gcgatacgga gaagtgtgtt gtaagcaatt ccgaggaagg cgaggtttcg
    10321 gatgtcctgg caacgatcgg aattttccat aagagaacgg acctgatcga agtcggttct
    10381 gaaaatttcg tcaagtctta ggattgaata cattaatatt taaacttacc tttcgaaggc
    10441 gagggcttgc ttggctctct ctccagcatc aacattttcc ttgcggatac gtctcattac
    10501 gagagatacg gcattagaat ctgatgggcg tggaagtcca gatcggcgat ggagcatatt
    10561 gagctgtccg agatgttgtt ggatcgtctt caccgccaat ccacgagctt ggaggtagag
    10621 caagtagtca cgaacgtctt ctggctcggc tgggaaccac ttgcggttgt taagcttgca
    10681 ccaggcagcc caagaacggc acactgagag aagcatcttc caggtgtgct cggagaaggc
    10741 ttgtctgtca cggaacatat ccatgaggtt cttgcgaact tcatcacttg tcgcgtcaac
    10801 tggaagtgct ggaagatttt gatgaacagt gagaagattt gacatatttt cgaagttttt
    10861 tagatgcact agaacaaagc gtgttggctt cctctgagcc cgctttcctt atatacccgc
    10921 attctgcagc cttacagaat gttctagaag gtcctagatg cattcgtttg aaaatactcc
    10981 cggtgggtgc aaagagacgc agacggaaaa tgtatctggg tctctttatt gtgtacacta
    11041 cttttccatg taccgaatgt gagtcgccct ccttttgcaa caagcagctc gaatgttcta
    11101 gaaaaaggtg gaaaatagta taaataccgt tgaaaataaa taccgaacaa catttgctct
    11161 aattgtgaaa ttagaaatct tcaaactata atcatgtcac tttaccacta tttccgtcca
    11221 gctcaacgtt ccgtttttgg tgatcatttt tgctttcgtc gtaaatctac acacgcgtct
    11281 cttccgtgcg agagtccaag ccagcagcca aattcgttga ctgagtattc aacgtttata
    11341 cgttgtcggc aacgagaaat aggaaaatgc atcgggaaat gttctttttt cgattttttc
    11401 caaggttttg acaaatttta ccacgaattt tgctatgttt tcaattaaaa aatatgttat
    11461 tcaactgttt ctatgaggaa aataaggctt tgcatgtaat tttcttattc agcataattt
    11521 ttaattaatt tgaattttct gtcctaacgt ttattttgtt ttcttggtta tgactgatct
    11581 gaaattaatt tttgaatttt aaggtaatat gtcaggcggt gccgcaagtt tgtacaaaaa
    11641 agcaggctcc atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
    11701 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
    11761 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
    11821 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
    11881 gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg
    11941 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga
    12001 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
    12061 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
    12121 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
    12181 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
    12241 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
    12301 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
    12361 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
    12421 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
    12481 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
    12541 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
    12601 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
    12661 gagggcaaag gaatagaccc agctttcttg tacaaagtgg gtccaattac tcttcaacat
    12721 ccctacatgc tctttctccc tgtgctccca ccccctattt ttgttattat caaaaaactt
    12781 ctcttaattt ctttgttttt tagcttcttt taagtcacct ctaacaatga aattgtgtag
    12841 attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca
    12901 ttaataataa ttctatccca aaatctacac aatgttctgt gtacacttct tatgtttttt
    12961 acttctgata aatttttttg aaacatcata gaaaaaaccg cacacaaaat accttatcat
    13021 atgttacgtt tcagtttatg accgcaattt ttaataactt cgtatagtat accttatacg
    13081 aagttatact agtatatatt tttatgtttt cttttaaacc ttcctccata tttctgaatt
    13141 gaatgttgtt gcatattgtg tgtccttatt tcatttccat catccttttg tgtgtttccc
    13201 ccttccacca attcccccca cgtcctaatg aaatgaaagc gagaagataa ataaacttgc
    13261 acattgtgtt cagatgagag gagcggaata cgaattggga gacggaaaga g
//
