LOCUS       sequence-211529-        8820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-regulated miR-E (miR-30 variant)-based RNAi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8820)
  AUTHORS   Fellmann C, Hoffmann T, Sridhar V, Hopfgartner B, Muhar M, Roth M, 
            Lai DY, Barbosa IA, Kwon JS, Guan Y, Sinha N, Zuber J
  TITLE     An optimized microRNA backbone for effective single-copy RNAi.
  JOURNAL   Cell Rep. 2013 Dec 26;5(6):1704-13. doi: 
            10.1016/j.celrep.2013.11.020. Epub 2013 Dec 12.
  PUBMED    24332856
REFERENCE   2  (bases 1 to 8820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.11.020"; journalName: "Cell Rep"; date: 
            "2013-12-26- 26"; volume: "5"; issue: "6"; pages: "1704-13"
COMMENT     Sequence Label: LT3GEP
FEATURES             Location/Qualifiers
     source          1..8820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    282..515
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             700..744
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1011..1128
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1144..1522
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    1152..1170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1188..1206
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1224..1242
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1260..1278
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1296..1314
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1332..1350
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1368..1386
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1431..1455
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(1546..1548,1549..1551,1552..2265)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1591..1612)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1852..1871)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2199..2220
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        2539..3038
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2574..2597)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2971..2990
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3059..3658
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3059..3078)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3555..3575
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3753..4341
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3806..3826)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4224..4235)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4548..4781
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4853..4974
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4890..4909)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4944..4963
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5014..5149
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5169..5188)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5170..5188)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5198..5215)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5198..5214)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5207..5229)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5356..5811
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5443..5462)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5653..5674
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5837..5941
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5942..6010,6011..6802)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6160..6179)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6973..7561
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7462..7481
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7715..7732
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7849..7870
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7885..7890,7891..7908,7909..7915)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7923..7939
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7928..7950
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7947..7963
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7947..7963
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7982..8002
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7984..8002
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8030..8256
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8257..8437
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8484..8609
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
       61 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      121 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      181 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
      241 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
      301 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
      361 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
      421 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
      481 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
      541 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
      601 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
      661 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
      721 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
      781 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
      841 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
      901 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
      961 agagacagat ccattcgatt agtgaacgga tctcgacggt atcggttaac ttttaaaaga
     1021 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     1081 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttat cgattctaga
     1141 ttcgagttta ctccctatca gtgatagaga acgtatgaag agtttactcc ctatcagtga
     1201 tagagaacgt atgcagactt tactccctat cagtgataga gaacgtataa ggagtttact
     1261 ccctatcagt gatagagaac gtatgaccag tttactccct atcagtgata gagaacgtat
     1321 ctacagttta ctccctatca gtgatagaga acgtatatcc agtttactcc ctatcagtga
     1381 tagagaacgt ataagcttta ggcgtgtacg gtgggcgcct ataaaagcag agctcgttta
     1441 gtgaaccgtc agatcgcctg gagcaattcc acaacacttt tgtcttatac caactttccg
     1501 taccacttcc taccctcgta aagtcgagct tgcgttggat ccaccatggt gagcaagggc
     1561 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     1621 cacaagttca gcgtgtctgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     1681 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     1741 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     1801 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     1861 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1921 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1981 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggcgaac
     2041 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     2101 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     2161 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     2221 accgccgccg ggatcactct cggcatggac gagctgtaca agtaatgacg tacggcggcc
     2281 gcctcgacta gggataacag ggtaattgtt tgaatgaggc ttcagtactt tacagaatcg
     2341 ttgcctgcac atcttggaaa cacttgctgg gattacttcg acttcttaac ccaacagaag
     2401 gctcgagcaa ccagaattcg agcaattatc ttgtttacta aaactgaata ccttgctatc
     2461 tctttgatac atttttacaa agctgaatta aaatggtata aattaaatca cttttttcaa
     2521 ttgacgcgta attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     2581 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     2641 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     2701 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     2761 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     2821 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     2881 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     2941 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     3001 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     3061 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     3121 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     3181 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     3241 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     3301 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     3361 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     3421 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     3481 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     3541 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     3601 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg
     3661 cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg catgtcgacc
     3721 tgcagccaag cttatcgaaa cgcgtctgga acaatcaacc tctggattac aaaatttgtg
     3781 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     3841 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     3901 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     3961 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     4021 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     4081 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     4141 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     4201 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     4261 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     4321 ccctttgggc cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg
     4381 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     4441 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     4501 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     4561 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     4621 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     4681 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     4741 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     4801 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     4861 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     4921 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     4981 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     5041 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     5101 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
     5161 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     5221 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     5281 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     5341 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     5401 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     5461 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     5521 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     5581 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     5641 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     5701 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     5761 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     5821 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     5881 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     5941 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     6001 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     6061 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     6121 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     6181 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     6241 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     6301 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     6361 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     6421 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     6481 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     6541 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     6601 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     6661 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     6721 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     6781 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     6841 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     6901 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     6961 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7021 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7081 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     7141 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7201 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7261 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     7321 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     7381 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     7441 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     7501 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     7561 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     7621 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     7681 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     7741 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     7801 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     7861 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     7921 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     7981 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
     8041 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
     8101 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
     8161 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
     8221 gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc
     8281 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     8341 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     8401 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     8461 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     8521 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     8581 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     8641 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     8701 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     8761 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
//
