LOCUS       sequence-211530-        9554 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Limb connective tissue specific inducible Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9554)
  TITLE     Cre-inducible plasmids
REFERENCE   2  (bases 1 to 9554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Prrx1:TFPnls-T2a-Cre-Ert
FEATURES             Location/Qualifiers
     source          1..9554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(37..465)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(147..168)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     359..378
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     592..614
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     606..623
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     607..623
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     656..675
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        656..674
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(689..700,701..888)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     intron          3462..3594
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3577..3596
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     3691..3710
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3758..3767
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3764..4471
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             4496..4516
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4520..4540
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4544..4564
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4586..4639
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4649..5671
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="NLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLS
                     VCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPR
                     PSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLG
                     IAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERW
                     ISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRY
                     LAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDG
                     D"
     CDS             5684..6619
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             5732..6625
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     primer_bind     complement(6709..6728)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6774..6829
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6828..6847)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     misc_feature    join(7174..7311,7312..7323)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7350..7370)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7350..7368)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7412..7428)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7412..7428)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7425..7447)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7436..7452
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7460..7466,7467..7484,7485..7490))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7505..7526
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7643..7660)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7814..8402)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7894..7913)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8573..9364,9365..9433))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9196..9215
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9434..9538)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
       61 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
      121 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
      181 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
      241 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
      301 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
      361 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
      421 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca
      481 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
      541 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
      601 gacgttgtaa aacgacggcc agtgagcgcg tagggataac agggtaatgc gcgcgtaata
      661 cgactcacta tagggcgaat tgggtaccca gaggtgtaaa gtacttgagt aattttactt
      721 gattactgta cttaagtatt atttttgggg atttttactt tacttgagta caattaaaaa
      781 tcaatacttt tacttttact taattacatt tttttagaaa aaaaagtact ttttactcct
      841 tacaatttta tttacagtca aaaagtactt attttttgga gatcacttgg gcccggctcg
      901 agcatgcacg cgtgaattca ataggagcct gtaattacgt tctagaaacc tgtagcaaga
      961 attcaatagg agcctgtaat tacgttctag aaacctgtag caagaattct tgctacaggt
     1021 ttctagaaca atgggggagg ggaagtcagc aagtggcagt agttggtttg cctctgacct
     1081 ttaggaaaat accagacatt tgcattttct atatatgcta gatgctatat ttgttattat
     1141 ctaaacaaat ttgctacaca gatgttagaa atgtatctaa atgttcccat agaaaccatt
     1201 aatacccagg ttgatactgt agcaattggt tgataggaac atgggtaacc agctgcggtt
     1261 tttcaaaatg tgcctgcttc ctggagaaaa ggcggaatgc aaggaaaggc acttaatgat
     1321 atttctgggg atggtctctg atctcttttc cttgacagtc ttctggagta tcaaccatac
     1381 tggctgcaaa cagaaacaag gatacttctt ggaggcaata tctagggtgc tagaagtcct
     1441 gatcttttac tcaggatttg gtcacaaatg gcaatcctca cgtctgaaag aaaaaggaat
     1501 ccatctgtgt aagtaaagat ggagcgcatg agcctccagt ctgagaagat atgggagaga
     1561 ctgatgctgt ctggccttag gaagagagaa agggtgtctc ctttccccta agggagcaga
     1621 gagttccaga gacaccggtg gaaatatgcc actttaacct gaccatcgag tcgcttccac
     1681 tccaggcact ttaggctcta ggagtctcac cgttggcatc tccgaaagca cctcaggata
     1741 ctcaagtccc cactctgtct ggcccaccca cgggcaagtc cccagcgcaa gaaccagctc
     1801 ttctggctta gatgccctcc ggcagacaaa ccttcgcggc tcagagcctg agctgccgag
     1861 caggggcgac cagagcactt tggcggggct gggggctgcc ggtcgggggc acggcacgag
     1921 tggctggagc gaggcgaggg gtggaaaaag gagggggcgg cgggtggact aatgatgaaa
     1981 aagtctcctc catcctggct ccttaattaa atgcatggaa agaaccgagg cgcgcacatc
     2041 tggtttcaat ctacagccct ttgatggcat caaatgttct tttcccagat cagggctgga
     2101 agttctgggc taactatggc cgtttggagc acagaaacca tttatacaca ctgggaccct
     2161 tccttctttt caattcaatc tcatagctac aggagaaggg gaaatctagc aatcagggga
     2221 gcgaggggaa acgtatgtat gtgtgtgttt aaagaacata aaacgccaca aataagcaca
     2281 tactatttta cttactggcc atatagtaac tcattcctaa tgagactatt aaagctgtta
     2341 ccagattcca acaggtcatg aaaacaccgt ccagcaccgc tctgccctct gtcaagcctg
     2401 gtcatagact tcgaccttcc tcagtctctg tgtttgccag ggcctcaccg aactggatac
     2461 agattagcac agagcccacc ccacccccag ctcctcctgc tccccccaac ttcttcagcc
     2521 ccagtgttcc tgtctgctct gatctggggc agcctcggct acgagcaaga aattagaaca
     2581 ttggcaaagg taggggaatt atcaggacgg actttcttct ggtttaatga atatgtaaat
     2641 tttctactgt ctgaagcctg gggatttctg gtggcagcga aagtctcgaa tccattcgaa
     2701 gctgaagact tctggatctg cccaagccgt ctttagatcg tagagagcct cccgttatta
     2761 tcgctcttgt tattttatta cattatttaa taggctgtct ctacagagcc aaacgagctt
     2821 taataggctt gctctttttt gccgttcagt ctcagactct ccctgcgctc tccgccaaaa
     2881 caaagctgca ggaaaagtta ctggggggcg gtgcagccgc agatttgact gttatagatg
     2941 tgcatctttt ccctgttcct ttttcttttt tcttttccta acaagtttgc cctcagctgt
     3001 tatcaaaaca tgcaaatgag atattccaag aggatctcaa acaaatccgg aggagttaat
     3061 gctgaaacaa aataatcagc cccgttttaa agtgactgcg ggtttcgggc acttttctct
     3121 ctgtgatact actgcaaggg aatgataatt agacctttat tttaaaaatc caaaggggct
     3181 ctctccttag cttccctccc ttttccctcc ctccctcctt cctccctccc tcctcctctc
     3241 ttgctccctc cctctccctt tctctctggc tctgatgttg gcaaaggggt tttcttaatc
     3301 agactgtttt ttcggtccga ggaaaaggag gtaggagatt gtgatggaga aagggggtct
     3361 gtaaaacgtc aggcgccgca caaaggcttt gccacgtaat tacaggctcc tattgaattc
     3421 gaattttaag ctgcagaagt tggtcgtgag gcactgggca ggtaagtatc aaggttacaa
     3481 gacaggttta aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt
     3541 tctgataggc acctattggt cttactgaca tccactttgc ctttctctcc acaggtgtcc
     3601 actcccagtt caattacagc tcttaaaatt ccgccactag agcctctgct aaccatgttc
     3661 atgccttctt ctctttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc
     3721 attttggcaa agaattattc cgctagcgct accggtcgcc accatggtga gcaagggcga
     3781 ggagaccaca atgggcgtaa tcaagcccga catgaagatc aagctgaaga tggagggcaa
     3841 cgtgaatggc cacgccttcg tgatcgaggg cgagggcgag ggcaagccct acgacggcac
     3901 caacaccatc aacctggagg tgaaggaggg agcccccctg cccttctcct acgacattct
     3961 gaccaccgcg ttcgcctacg gcaacagggc cttcaccaag taccccgacg acatccccaa
     4021 ctacttcaag cagtccttcc ccgagggcta ctcttgggag cgcaccatga ccttcgagga
     4081 caagggcatc gtgaaggtga agtccgacat ctccatggag gaggactcct tcatctacga
     4141 gatacacctc aagggcgaga acttcccccc caacggcccc gtgatgcaga agaagaccac
     4201 cggctgggac gcctccaccg agaggatgta cgtgcgcgac ggcgtgctga agggcgacgt
     4261 caagcacaag ctgctgctgg agggcggcgg ccaccaccgc gttgacttca agaccatcta
     4321 cagggccaag aaggcggtga agctgcccga ctatcacttt gtggaccacc gcatcgagat
     4381 cctgaaccac gacaaggact acaacaaggt gaccgtttac gagagcgccg tggcccgcaa
     4441 ttccaccgac ggcatggacg agctgtacaa gtccggactc agatctcgag ctgatccaaa
     4501 aaagaagaga aaggtagatc caaaaaagaa gagaaaggta gatccaaaaa agaagagaaa
     4561 ggtaggatcc accggatcta gacccgaggg cagaggaagt cttctaacat gcggtgacgt
     4621 ggaggagaat cccggcccac tagtcgacaa tttactgacc gtacaccaaa atttgcctgc
     4681 attaccggtc gatgcaacga gtgatgaggt tcgcaagaac ctgatggaca tgttcaggga
     4741 tcgccaggcg ttttctgagc atacctggaa aatgcttctg tccgtttgcc ggtcgtgggc
     4801 ggcatggtgc aagttgaata accggaaatg gtttcccgca gaacctgaag atgttcgcga
     4861 ttatcttcta tatcttcagg cgcgcggtct ggcagtaaaa actatccagc aacatttggg
     4921 ccagctaaac atgcttcatc gtcggtccgg gctgccacga ccaagtgaca gcaatgctgt
     4981 ttcactggtt atgcggcgga tccgaaaaga aaacgttgat gccggtgaac gtgcaaaaca
     5041 ggctctagcg ttcgaacgca ctgatttcga ccaggttcgt tcactcatgg aaaatagcga
     5101 tcgctgccag gatatacgta atctggcatt tctggggatt gcttataaca ccctgttacg
     5161 tatagccgaa attgccagga tcagggttaa agatatctca cgtactgacg gtgggagaat
     5221 gttaatccat attggcagaa cgaaaacgct ggttagcacc gcaggtgtag agaaggcact
     5281 tagcctgggg gtaactaaac tggtcgagcg atggatttcc gtctctggtg tagctgatga
     5341 tccgaataac tacctgtttt gccgggtcag aaaaaatggt gttgccgcgc catctgccac
     5401 cagccagcta tcaactcgcg ccctggaagg gatttttgaa gcaactcatc gattgattta
     5461 cggcgctaag gatgactccg gtcagagata cctggcctgg tctggacaca gtgcccgtgt
     5521 cggagccgcg cgagatatgg cccgcgctgg agtttcaata ccggagatca tgcaagctgg
     5581 tggctggacc aatgtaaata ttgtcatgaa ctatatccgt aacctggata gtgaaacagg
     5641 ggcaatggtg cgcctgctgg aagatggcga tctcgagcca tctgctggag acatgagagc
     5701 tgccaacctt tggccaagcc cgctcatgat caaacgctct aagaagaaca gcctggcctt
     5761 gtccctgacg gccgaccaga tggtcagtgc cttgttggat gctgagcccc ccatactcta
     5821 ttccgagtat gatcctacca gacccttcag tgaagcttcg atgatgggct tactgaccaa
     5881 cctggcagac agggagctgg ttcacatgat caactgggcg aagagggtgc caggctttgt
     5941 ggatttgacc ctccatgatc aggtccacct tctagaatgt gcctggctag agatcctgat
     6001 gattggtctc gtctggcgct ccatggagca cccagtgaag ctactgtttg ctcctaactt
     6061 gctcttggac aggaaccagg gaaaatgtgt agagggcatg gtggagatct tcgacatgct
     6121 gctggctaca tcatctcggt tccgcatgat gaatctgcag ggagaggagt ttgtgtgcct
     6181 caaatctatt attttgctta attctggagt gtacacattt ctgtccagca ccctgaagtc
     6241 tctggaagag aaggaccata tccaccgagt cctggacaag atcacagaca ctttgatcca
     6301 cctgatggcc aaggcaggcc tgaccctgca gcagcagcac cagcggctgg cccagctcct
     6361 cctcatcctc tcccacatca ggcacatgag taacaaaggc atggagcatc tgtacagcat
     6421 gaagtgcaag aacgtggtgc ccctctatga cctgctgctg gaggcggcgg acgcccaccg
     6481 cctacatgcg cccactagcc gtggaggggc atccgtggag gagacggacc aaagccactt
     6541 ggccactgcg ggctctactt catcgcattc cttgcaaaag tattacatca cgggggaggc
     6601 agagggtttc cctgccacag cttaagatat cactcctcag gtgcaggctg cctatcagaa
     6661 ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc
     6721 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
     6781 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     6841 atatgggagg gcaaatcatt taaaacatca gaatgagcat ttggtttaga gtttggcaac
     6901 atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat
     6961 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat
     7021 tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca
     7081 tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct
     7141 tctcttatga agatccctcg accagcggcc gctaatactc aagtacaatt ttaatggagt
     7201 acttttttac ttttactcaa gtaagattct agccagatac ttttactttt aattgagtaa
     7261 aattttccct aagtacttgt actttcactt gagtaaaatt tttgagtact ttttacacct
     7321 ctgccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcattacc
     7381 ctgttatccc tacgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7441 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7501 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7561 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7621 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7681 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7741 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7801 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7861 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7921 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7981 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8041 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8101 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8161 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8221 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     8281 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8341 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8401 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8461 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8521 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8581 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8641 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8701 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8761 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8821 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8881 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8941 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9001 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9061 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9121 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9181 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9241 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9301 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9361 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9421 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9481 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9541 catttccccg aaaa
//
