LOCUS       sequence-211531-        4399 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid serves as a backbone for heterologous expression in the 
            model archaeal organism Methanococcus maripaludis..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Lyu Z, Jain R, Smith P, Fetchko T, Yan Y, Whitman WB
  TITLE     Engineering the Autotroph Methanococcus maripaludis for Geraniol 
            Production.
  JOURNAL   ACS Synth Biol. 2016 Jul 15;5(7):577-81. doi: 
            10.1021/acssynbio.5b00267. Epub 2016 Feb 17.
  PUBMED    26886063
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00267"; journalName: "ACS Synth Biol"; date: 
            "2016-07-15- 15"; volume: "5"; issue: "7"; pages: "577-81"
COMMENT     Sequence Label: pMEV4
FEATURES             Location/Qualifiers
     source          1..4399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     34..53
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    1328..1348
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     rep_origin      complement(2463..3051)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2543..2562)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3222..4013,4014..4082))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3845..3864
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4083..4187)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4255..4273
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4311..4333)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
       61 tgcaccaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaccccc
      121 agtgaattaa aatatataaa aaaaggaaac catattaggt ttcctttagt tttcttttac
      181 tattttaccc aatctaataa taaattaata ataaattagt aattaactta gattttagca
      241 ggtaagatag ggtctctttc accagcagct tcgaattaat tcccgatccg ctcctgggca
      301 ccgaactgcg ccgcgtgttc agcagggtcg gcgtgttcgg tgtgtccccc gcggtgggcc
      361 tcgggggcgg gtgcggggtc ggcggggccg ccccgggtgg cttcggtcgg agccatggtt
      421 atgctcctgg ttttcttgtc atacaccatg ttcttggtcc ttctggaact tcaacatctg
      481 ctgtaactgt aaatccaagt ctttcataaa atggaagatt tcttggtgct gatgtttcta
      541 aaaatgctgg aactcctgct ctttcagctg cttcaacacc aggaagtaca acagcagaac
      601 ctaaaccttt tccctggtga tctggtgaaa ctccaactgt tgctaaaaac catgctggtt
      661 cttttggtct gtgtggtgca agtaatcctt ccatctgttg ctgagctgct aatcttgatc
      721 ctgataattc agccattctt ggtccaattt ctgcaaaaac agcaccagct tcaactgatt
      781 ctggtgttgt ccaaactgca actgctgctc catcatctgc aacccatact ttacctatat
      841 caagaccaac tcttgttaaa aataattctt gtaattctgt aactctttca atgtgtctat
      901 ctggatcaac tgtgtgtctt gttgctggat aatctgcaaa agctgctgca agagttctta
      961 ctgccctagg tacgtcatct cttgttgcta atctaactgt tggtttatat tctgtcatgg
     1021 aaggtcgtct cctctagttt tttttgatat atacatcata acattactct atgtatatat
     1081 attcactttt tcattaacat taaatagaaa agtttatata taagatgtta ataacacaat
     1141 aatttgaatt tgaatactca aaaaatgggc tttaatatat aaaattaaga tgaaaataga
     1201 tgatttttta aaaaaatgtt attattatat ctcaatatct aaatattaga ttaatattaa
     1261 ttattaccca aatatttcaa tgaatattta gttttgaata gtatattacg aatagggcgt
     1321 tttttatctg cagcggccgc tactagtatt agttatctat aaaattataa tatcaatagc
     1381 ttattagcta aattttaaca aatttcataa gccaatattt tacaattgtt tatataaaat
     1441 atgcctttaa attatttaaa cctatgttat ttatgttata ttttgatcga tcagctgaat
     1501 tatctagtaa ttcttatctc gtctagaacc gggcgcgaat tcatctagta attcttatct
     1561 cgtaacttaa aaaaccttgg atttttgaag tatgcaggaa agaaggaagg atattaccag
     1621 ataacaaaga aaggaaagga caaaataaag gaaatgaaaa aagcatacag ttatacaagt
     1681 ttaagcatga aagaaagaag agcatatcaa gatacatatt aaactaacat tttaataata
     1741 atgtttgttt aactttttcg gacacttttt ttaaatcttt tttatttttt acaatatgta
     1801 aaatatgttc gattttttac aagaaatcaa aaaaagtaat aataaatatt attcaataat
     1861 caatacaagg ttaaatataa tataatatat aaaaaaatat cgaacatatc gaacatatta
     1921 tatatctata ttataataga ttattttttt tatatctctt caaattgtct ttcaaaatgt
     1981 tcgatttttt tatcgaacat actcgcacac atcgaacatt ataaaaaatg ttcgaaaaat
     2041 gttcgatttt tttatcgaac atttttgggg tatatcccgc aggtcttggc cctgtttaaa
     2101 ccctataaat agactttttc aaaatgttcg atttttcccc gctgggtgta tgtttttttt
     2161 tcaaatattt ttcaggtatt tttccagtaa tatttctgaa aattaaaaat attattttca
     2221 tctgaaggag actacacgat atcgtgtatt tcgctccagg acaaacgcac ttctaaaact
     2281 gcaataaaaa aaaactaaat tatagatacg atatataaat taaaatctgt tttcattctc
     2341 tgttttcttc tgaaaggggg tttatacgtt gtggtgataa aaaaaaacta aataatgggt
     2401 accatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2461 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2521 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2581 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2641 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2701 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2761 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     2821 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     2881 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     2941 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     3001 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     3061 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     3121 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     3181 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     3241 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     3301 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     3361 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     3421 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     3481 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     3541 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     3601 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     3661 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     3721 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     3781 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     3841 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     3901 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     3961 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     4021 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     4081 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     4141 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     4201 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     4261 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     4321 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     4381 cgtcagggcg cgtcagcgg
//
